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Positive identity as a positive youth development construct: conceptual bases and implications for curriculum development.

According to the theories of Erikson, Marcia, and Higgins, adolescents who can cultivate a clear and positive identity after their developmental struggles in adolescence often advance more smoothly into adulthood. Identity is organized, learned, and dynamic, and subjective evaluation of an individual's identity has emotional consequences for that individual. This paper discusses literature on the nature and importance of identity and presents strategies such as self-esteem enhancement, management of exploration and commitment, as well as reduction of self-discrepancies to help adolescents develop functional identity. Significant determinants of a person's identity development, namely gender and cultural issues, are also addressed as they are important in designing the "Positive Identity Development" package of Positive Adolescent Training through Holistic Social Programmes (P.A.T.H.S.), supported by the Hong Kong Jockey Club Charities Trust, to help adolescents develop a positive identity.

Adolescent↗

A father's abdication: Lear's retreat from 'aesthetic conflict'.

The author explores the potential contribution of Shakespeare's 'King Lear' to psychoanalytic thinking, linking a reading of the play focused on the emotional tensions inherent in the parental function of endowing ('heriting') the next generation with the developmental struggle characterised by Donald Meltzer as the 'aesthetic conflict'. Following Meltzer's definition of passion as the 'consortium' of Bion's emotional links, love, hate and the urge to know (L, H and K), the author develops an understanding of 'aesthetic conflict' linked with the tension inherent in that constellation. It is suggested that L and H split off from each other and from K become attempts to possess and control, while K split off from L and H becomes an attack on dreaded emotional links, oscillating between attempting to ignore them and attempting to overcome them. The author suggests an affinity between Bion's K link and what in 'King Lear' is pictured as a capacity to 'see feelingly' in the context of the struggle to give the object its freedom. This way of characterising 'aesthetic conflict' is linked with a fresh look at weaning as a lifelong developmental process, which in turn leads to a reconsideration of the psychoanalytic models of the dynamics of mourning.

Conflict, Psychological↗

"Speak to me as to thy thinkings" commentary on 'Interpersonal psychoanalysis' radical façade" by Irwin Hirsch.

This article has two purposes. First, a rebuttal to those writers, including Irwin Hirsch, who criticize the current emphasis by relational analysts on the value of affective openness and affective honesty, particularly with regard to their use of clinical vignettes that vividly portray the analyst's use of self-revelation--as if these illustrations were revealing an endorsement of a naive and mindless invasion of the patient's psyche. The second, and perhaps more important purpose, is to illuminate something I feel is obscured by Hirsch's framing the topic of the analyst's "spontaneity" in the context of analytic politics--that an analyst's self-revelation in language is increasingly understood to be not simply "allowable," but a necessary part of the clinical process. Language does not make it less spontaneous nor part of what Hirsch calls a "standardized technique." Its most powerful therapeutic contribution is in facilitating linguistic symbolization of dissociated, enacted, subsymbolic experience that is immune to self-reflective cognition, immune to internal conflict, and thereby unavailable to interpretation until it becomes relationally accessible to language and thought. Both neuroscience and cognitive research support the need for a revised theory of therapeutic action consistent with the growing recognition of the human mind as a nonlinear, self-organizing dynamic system-a system in which normal maturation as well as therapeutic repair depends, developmentally, on an ineffable coming together of two minds in an unpredictable way. From this vantage point, the analyst's self-revelation contributes to the coconstruction of an alive intersubjective space through an ongoing process of engagement between two subjectivities, making the analyst's subjective openness as potentially negotiable as any other aspect of the patient/analyst relationship, rather than an unrepairable "intrusion" into a self-contained psyche.

Affect↗

A developmental study of the perception of onset spectra for stop consonants in different vowel environments.

The importance of different acoustic properties for the perception of place of articulation in prevocalic stop consonants was investigated from a developmental perspective. Eight adults and eight children in each of the age groups, 5, 6, 7, 9, and 11 years, listened to synthesized syllables comprised of all combinations of [b d g] and [i a]. The synthesis parameters were adapted from Blumstein and Stevens [J. Acoust. Soc. Am. 67, 648-662 (1980)], and included manipulations of the following stimulus variables: formant transitions (moving or straight), noise burst (present or absent), and voicing duration (10 or 46 ms). Identification performance was high for all age groups across most stimulus types. Formant transition motion generally was not necessary for accurate identification, and there was no difference between age groups in terms of the perceptual weight placed on this cue. Furthermore, the results did not support the salience of duration as a developmental cue to place of articulation. The presence of a burst improved identification for the velar and alveolar places of articulation for all age groups, but was particularly important for the 11-year-olds and adults. These findings indicate that children, by age 5, do not rely on dynamic formant motion any more than adults do, and that the ability to integrate acoustic cues across regions of spectral change shows developmental patterns.

Adult↗

A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1.

ARF-like proteins (ARLs) are distinct group of members of the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was more abundant in liver than in other adult tissues tested. mARL5, similar to mARL4, was developmentally regulated and localized to nuclei. hARL5 interacted with importin-alpha through its C-terminal bipartite nuclear localization signal. When expressed in COS-7 cells, mutant hARL5(T35N), which is predicted to be GDP bound, was concentrated in nucleoli. The N-terminus of hARL5, like that of ARF, was myristoylated. Yeast two-hybrid screening and in vitro protein-interaction assays showed that hARL5(Q80L), predicted to be GTP bound, interacted with heterochromatin protein 1alpha (HP1alpha), which is known to be associated with telomeres as well as with heterochromatin, and acted as a transcriptional suppressor in mammalian cells. The interaction was reproduced in COS cells, where hARL5(Q80L) was co-immunoprecipitated with HP1alpha. hARL5 interaction with HP1alpha was dependent on the nucleotide bound, and required the MIR-like motif. Moreover, hARL5(Q80L), but not hARL5 lacking the MIR-like motif, was partly co-localized with overexpressed HP1alpha. Our findings suggest that developmentally regulated ARL5, with its distinctive nuclear/nucleolar localization and interaction with HP1alpha, may play a role(s) in nuclear dynamics and/or signaling cascades during embryonic development.

ADP-Ribosylation Factors↗

The origin of hematopoietic cell type diversity.

The hematopoietic system remains robust with regards to extrinsic perturbations, in sharp contrast with the stochastic behavior of hematopoeitic stem cells (HSCs) at the single cell level, suggesting that stability may be achieved within a stem cell system that undergoes constant self-renewal, commitment to differentiation and generates cell type diversification. Converging evidence at the interface of cellular, molecular and numerical studies suggests that diversity is generated by the chaotic dynamics of transcription factor networks within a cell and of the combination of growth factors and cytokines in the environment, both involving cooperation and competition. Current evidence indicates that HSCs are primed for multilineage gene expression. A subtle shift in transcription factor dosage is sufficient to perturb this equilibrium and to drive lineage commitment that involves a resolution of complexity at the molecular level and a transition towards less chaotic behavior. This dynamical instability establishes a state of responsiveness to extrinsic signals. Evolutionary conserved environmental cues that drive pattern formation or migratory behavior during embryonic development operate in the adult to influence the decision between self-renewal and differentiation in HSCs, as exemplified by the role of Notch1, Wnt proteins, BMPs and VEGF. In contrast, a network of cytokines uniquely present in mammalians influences later developmental stages, from progenitors with more restricted potentials (tri-, bi- or unipotent) to mature functional cells. These cytokines have co-opted the ancient Jak-STAT pathway but also appear to trigger lineage-affiliated transcription factors, thus linking environmental signaling to cell fate decisions.

Acute Disease↗

Dictyostelium discoideum: cellular self-organization in an excitable biological medium.

The dynamics which govern the establishment of pattern and form in multicellular organisms remain a key problem of developmental biology. We study this question in the case of morphogenesis during aggregation of the slime mould Dictyostelium discoideum. Here detailed experimental information allows the formulation of a mechanistic model in which the central element is the coupling of the previously much-studied intracellular cyclic AMP signalling with the chemotactic cell response in cyclic AMP gradients. Numerical simulations of the model show quantitatively how signal relay, chemotactic movement and adaptation orchestrate the collective modes of cell signalling and migration in the aggregating cell layer. The interaction of chemotaxis with the cyclic AMP excitation waves causes the initially homogeneous cell layer to become unstable towards the formation of a branching cell stream pattern with close cell-cell contacts as observed in situ. The evolving cell morphology in turn leads to a pattern of non-homogeneous excitability of the medium and thus feeds back into the cAMP dynamics. This feedback can explain the decrease in signalling period and propagation speed with time, as well as observations on the structure of the spiral wave core in this self-organized excitable medium.

Animals↗

Dynamic synaptic modification threshold: computational model of experience-dependent plasticity in adult rat barrel cortex.

Previous electrophysiological experiments have documented the response of neurons in the adult rat somatic sensory ("barrel") cortex to whisker movement after normal experience and after periods of experience with all but two whiskers trimmed close to the face (whisker "pairing"). To better understand how the barrel cortex adapts to changes in the flow of sensory activity, we have developed a computational model of a single representative barrel cell based on the Bienenstock, Cooper, and Munro (BCM) theory of synaptic plasticity. The hallmark of the BCM theory is the dynamic synaptic modification threshold, theta M, which dictates whether a neuron's activity at any given instant will lead to strengthening or weakening of the synapses impinging on it. The threshold theta M is proportional to the neuron's activity averaged over some recent past. Whisker pairing was simulated by setting input activities of the cell to the noise level, except for two inputs that represented untrimmed whiskers. Initially low levels of cell activity, resulting from whisker trimming, led to low values for theta M. As certain synaptic weights potentiated, due to the activity of the paired inputs, the values of theta M increased and after some time their mean reached an asymptotic value. This saturation of theta M led to the depression of some inputs that were originally potentiated. The changes in cell response generated by the model replicated those observed in in vivo experiments. Previously, the BCM theory has explained salient features of developmental experience-dependent plasticity in kitten visual cortex. Our results suggest that the idea of a dynamic synaptic modification threshold, theta M, is general enough to explain plasticity in different species, in different sensory systems, and at different stages of brain maturity.

Animals↗

Income poverty, poverty co-factors, and the adjustment of children in elementary school.

Since 1990, there have been great advances in how developmental researchers construct poverty. These advances are important because they may help inform social policy at many levels and help frame how American culture constructs poverty for children, both symbolically and in the opportunities children and families get to escape from poverty. Historically, developmental perspectives have embodied social address and main effects models, snapshot views of poverty effects at single points in time, and a rather narrow focus on income as the symbolic marker of the ecology of disadvantage. More recent views, in contrast, emphasize the diverse circumstances of disadvantaged families and diverse outcomes of disadvantaged children, the multiple sources of risk and the multiple determinants of poor outcomes for these children, dynamic aspects of that ecology, and change as well as continuity in outcome trajectories. The advances also consist of more powerful frames for understanding the ecology of disadvantage and the risk it poses for child outcomes. Most developmental researchers still tend to frame causal variables ultimately in terms of the dichotomy between social causation and social selection views, with a primary emphasis on the former. In part, this framing has reflected limitations of sample size and design, because the theoretical and empirical power of reciprocal selection models is clear (Kim et al., 2003). The conceptual advances that prompt such models include widespread acknowledgement of third variable problems in interpreting effects, of the clear need for multivariate approaches, and the need to pursue mechanisms and moderators of the relations between causal candidates and child outcomes. In the context of these advances, one of the core goals of our research program has been to construct robust representations of environmental adversity for disadvantaged families. Most of our research focuses on contextual co-factors at a family level (e.g., maternal relationship instability), which either have not been described by many researchers or have been described in a way that does not fit the ecology of disadvantage (e.g., marital status). We found that income poverty, key contextual co-factors, and endogenous child attributes tend to show independent and selective associations with child academic competence and externalizing behavior, and that co-factor effects tend to be direct rather than mediated by harsh parenting, tend to have effects that are episodic and concurrent, and are easily- and well-represented by multiple risk indexes that bear powerful relations to child problem behaviors. A second core goal has been to better understand the developmental construction of poor outcomes for disadvantaged children, which requires consideration of dynamic aspects of the ecology and the potential importance of the timing of risk experiences. We found that family instability and change in environmental circumstances predict increases in problem behaviors, and that dose of adversity seems to matter for some variables if it is recent, and not for other variables. Through person-centered research, we also are beginning to understand some factors that seem to underlie the convergence of adjustment problems over grade in school. Many of our co-factor findings and many of our developmental findings seem both complex and double-edged. One edge is that they encourage a certain pessimism in showing how environmental adversity progressively constructs poor outcomes for disadvantaged children in school. Overall, for instance, we saw more problems and more multi-dimensional problems in fifth grade than in first grade, and the impact of environmental change was mostly negative. The other edge, however, is more positive in reflecting the possibility of discontinuity in child adjustment problems associated with positive changes in family circumstances. Findings for minimal persistence and for the strength of recent and concurrent effects argue that the possibility of self-righting and emergent competence in school is robust through the fifth grade even for the most problematic disadvantaged children.

Achievement↗

Effects of developmental and functional interactions on mouse cranial variability through late ontogeny.

The mammalian skull performs a variety of functions and its growth and development mirrors this complexity. Cranial growth and development have been actively studied for many years. Despite this interest, the variation in the patterns and processes of skull growth has attracted little attention. An important and unanswered question is the extent to which patterns of cranial covariation and variation are dynamically reworked throughout postnatal growth. To address this question, we examine patterns of variability in random-bred mouse skulls aged 35, 90, and 150 days. Using a battery of both Procrustes coordinate and Euclidean distance-based methods, we measure mean shape, canalization, developmental stability, and morphological integration in these skulls. We predict that the patterns of variability are dynamic, particularly between the youngest and the two oldest age groups due to the influence of functional effects such as postweaning mastication. We also hypothesize that patterns of variability are structured by the same functional and developmental factors that have been shown to influence cranial growth in primates. Our results indicate that contrary to our predictions, patterns of canalization, developmental stability, and morphological integration are stabilized before 35 days. The mean shape, however, changed significantly with growth. We found that only the facial region showed significant integration as predicted by the functional matrix model used in other studies of integration. These results indicate that phenotypic integration in these mice does not closely match those found for primate species, suggesting that comparisons between species should be made with care.

Age Factors↗

Dynamic changes in ovarian c-kit and Steel expression during the estrous reproductive cycle.

W and Steel mutant mice exhibit similar developmental defects in melanogenesis, haematopoiesis, and gametogenesis. Consistent with the cell autonomous and microenvironmental nature of W and Sl mutations, respectively, W encodes the c-kit receptor tyrosine kinase while Steel encodes the Kit ligand. Both c-kit and Steel are expressed in various cells in which no corresponding mutant phenotype has yet been demonstrated. In the adult ovary, certain stromal-derived cells (theca and interstitial), as well as oocytes, express c-kit, while granulosa cells express Steel. We show here that the cessation of oocyte growth, at the transition of the follicle to the antral stage, is associated with the cessation of Steel expression in the cumulus granulosa cells in the vicinity of the oocyte. These observations suggest a role for the Kit signaling pathway in oocyte growth or in meiotic arrest. In addition, the cyclic secretion of luteinizing hormone immediately and dramatically results in elevated Steel expression in mural granulosa cells and decreased levels of c-kit transcripts in stromal-derived cells. This influence of the estrous reproductive cycle on c-kit/Steel expression suggests that the Kit signaling pathway, in addition to its previously described role in primordial germ cell development, is involved in follicular development in the adult female.

Animals↗

Dynamic EGFR-Ras signalling in Drosophila leg development.

In Drosophila, as in many other animals, EGFR-Ras signalling has multiple developmental roles from oogenesis to differentiation. In leg development, in particular, it has been described to be responsible for the establishment of distal leg fates in a graded manner. Here, we investigate the patterns of expression of activators of EGFR-Ras signalling, as well as some of the effectors, in order to better understand the patterning of the distal leg, and to investigate further roles of this signalling pathway. These patterns, together with genetic data obtained by different mutant conditions for EGFR-Ras members and transgene expression, suggest two rounds of signalling in leg development. Early, the EGFR ligand Vein is the main player in distal leg patterning, possibly supported later by another ligand activated by Rhomboid. Later, in a second wave of signalling when all the proximal-distal leg fates have been specified, domains of EGFR/Ras activation appear inside each leg segment to regulate Notch-mediated joint development, and also some organs such as tendons and sensory organs. This second wave relies on a ligand activated by Rhomboid.

Animals↗

[Effect of the cultivation temperature on the dynamics of the ATP and ADP levels and on the growth of P. nigricans Thom. Strains in the presence of various carbon sources].

The effect of the medium high temperature (28--28.6 degrees C) on the yield of the dry mycelium and the levels of ATP and ADP in the cells of the highly and low productive strains of P. nigricans Thom, 117 and B at different developmental periods (2, 5, 9 and 13 days) on the mineral medium using glucose, succinate or acetate as the carbon sources was studied. It was shown that the effect of the high temperature depended on the strain and the carbon source. The growth stimulating effect of this factor observed in strain 117 grown on the media with the above carbon sources and strain B grown on the media with glucose and acetate as the carbon sources was evident at different periods and later replaced by suppression. The growth of strain 177 in the presence of succinate was not inhibited, while that of strain B was suppressed during the whole developmental cycle. Such an effect of high temperature on the culture growth was the result of different supply of energy in the form of ATP to the strains which was confirmed by the study of the dynamics of the levels of ATP and ADP and their ratio in the strains during the developmental process.

Adenosine Diphosphate↗

The dynamics of dendritic structure in developing hippocampal slices.

Time-lapse fluorescence confocal microscopy was used to directly visualize the formation and dynamics of postsynaptic target structures (i.e., dendritic branches and spines) on pyramidal neurons within developing tissue slices. Within a 2 week period of time, pyramidal neurons in cultured slices derived from early postnatal rat (postnatal days 2-7) developed complex dendritic arbors bearing numerous postsynaptic spines. At early stages (1-2 d in vitro), many fine filopodial protrusions on dendrite shafts rapidly extended (maximum rate approximately 2.5 microM/minute) and retracted (median filopodial lifetime, 10 min), but some filopodia transformed into growth cones and nascent dendrite branches. As dendritic arbors matured, the population of fleeting lateral filopodia was replaced by spine-like structures having a low rate of turnover. This developmental progression involved a transitional stage in which dendrites were dominated by persistent (up to 22 hr) but dynamic spiny protrusions (i.e., protospines) that showed substantial changes in length and shape on a timescale of minutes. These observations reveal a highly dynamic state of postsynaptic target structures that may actively contribute to the formation and plasticity of synaptic connections during CNS development.

Animals↗

Characterization of antirrhinum petal development and identification of target genes of the class B MADS box gene DEFICIENS.

The class B MADS box transcription factors DEFICIENS (DEF) and GLOBOSA (GLO) of Antirrhinum majus together control the organogenesis of petals and stamens. Toward an understanding of how the downstream molecular mechanisms controlled by DEF contribute to petal organogenesis, we conducted expression profiling experiments using macroarrays comprising >11,600 annotated Antirrhinum unigenes. First, four late petal developmental stages were compared with sepals. More than 500 ESTs were identified that comprise a large number of stage-specifically regulated genes and reveal a highly dynamic transcriptional regulation. For identification of DEF target genes that might be directly controlled by DEF, we took advantage of the temperature-sensitive def-101 mutant. To enhance the sensitivity of the profiling experiments, one petal developmental stage was selected, characterized by increased transcriptome changes that reflect the onset of cell elongation processes replacing cell division processes. Upon reduction of the DEF function, 49 upregulated and 52 downregulated petal target genes were recovered. Eight target genes were further characterized in detail by RT-PCR and in situ studies. Expression of genes responding rapidly toward an altered DEF activity is confined to different petal tissues, demonstrating the complexity of the DEF function regulating diverse basic processes throughout petal morphogenesis.

Antirrhinum↗

Transcriptional profiling of wheat caryopsis development using cDNA microarrays.

The expression of 7,835 genes in developing wheat caryopses was analyzed using cDNA arrays. Using a mixed model analysis of variance (ANOVA) method, 29% (2,237) of the genes on the array were identified to be differentially expressed at the 6 different time-points examined, which covers the developmental stages from coenocytic endosperm to physiological maturity. Comparison of genes differentially expressed between two time-points revealed a dynamic transcript accumulation profile with major re-programming events that occur at 3-7, 7-14 and 21-28 DPA. A k-means clustering algorithm grouped the differentially expressed genes into 10 clusters, revealing co-expression of genes involved in the same pathway such as carbohydrate and protein synthesis or preparation for desiccation. Functional annotation of genes that show peak expression at specific time-points correlated with the developmental events associated with the respective stages. Results provide information on the temporal expression during caryopsis development for a significant number of differentially expressed genes with unknown function.

DNA, Complementary↗

A C. elegans E/Daughterless bHLH protein marks neuronal but not striated muscle development.

The E proteins of mammals, and the related Daughterless (DA) protein of Drosophila, are ubiquitously expressed helix-loop-helix (HLH) transcription factors that play a role in many developmental processes. We report here the characterization of a related C. elegans protein, CeE/DA, which has a dynamic and restricted distribution during development. CeE/DA is present embryonically in neuronal precursors, some of which are marked by promoter activity of a newly described Achaete-scute-like gene hlh-3. In contrast, we have been unable to detect CeE/DA in CeMyoD-positive striated muscle cells. In vitro gel mobility shift analysis detects dimerization of CeE/DA with HLH-3 while efficient interaction of CeE/DA with CeMyoD is not seen. These studies suggest multiple roles for CeE/DA in C. elegans development and provide evidence that both common and alternative strategies have evolved for the use of related HLH proteins in controlling cell fates in different species.

Amino Acid Sequence↗