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[Epizootiological research on salmonellosis in swine].

The epizootiology of Salmonella infections in swine was studied in 1970-1980 in 7 districts of this country on a number of farms and industrial complexes in order to elucidate some aspects of the epizootiologic process. A total of 26,957 samples taken from swine and the environment were studied bacteriologically. An industrial complex in each district served as a test one to investigate samples from various groups of swine in the technologic process. It was found that salmonelloses did exist, and Salmonella organisms were isolated from pigs on all studied farms and complexes, however, no diseases and organisms were recorded with both animals and the environment in Swine Nucleus Bases. On the complexes Salmonellae were isolated from pigs of all technologic groups, and, in most cases, the Salmonella species coincided by sows and pigs. This explained the stationary character of the disease on some complexes where a sui generis epizootic process ran its course, exhibiting particular aspects and correlations. Salmonella organisms were isolated in 0.95 per cent of the investigated samples from the test groups on the test complexes as well as in 4.13 per cent of the groups out of the experiment. In samples taken from other complexes, farms of AIC, IAC, auxilliary farms, etc., there were positive findings in 5.32 per cent of the cases. Enzootics on the complexes were most common with pigs aged 40-50 days as against farms with traditional types of technology where 2-4-month-old pigs were involved.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Therapeutic effect of lincomycin and spectinomycin water medication on swine dysentery.

The therapeutic effects of various water medications on swine dysentery were determined in 223 pigs under controlled conditions. Carrier pigs were mixed with test animals until the disease was established. Lincomycin (22 mg/liter), spectinomycin (44 mg/liter) alone and lincomycin and spectinomycin in combination (66 mg/liter) and sodium arsanilate (161 mg/liter) in drinking water for seven days were the drugs evaluated. Negative and positive controls were also included. The experiment was terminated 41 to 43 days after initial medication. Mortality, mean value for stool consistency, incidence of dysenteric days and gross lesions of swine dysentery were the parameters measured for each treatment group.The lincomycin-spectinomycin water medication was effective for the treatment of swine dysentery. Pigs treated with lincomycin-spectinomycin had a higher survival rate, a lower incidence of dysenteric days and fewer gross lesions of swine dysentery than pigs treated with sodium arsanilate, lincomycin or spectinomycin alone or the infected controls (P < 0.05).

Animals↗

Swine dysentery: studies of gnotobiotic pigs inoculated with Treponema hyodysenteriae, Bacteroides vulgatus, and Fusobacterium necrophorum.

Transmission experiments were carried out in gnotobiotic pigs to determine whether lesions typical of swine dysentery could be produced by oral inoculation of Treponema hyodysenteriae in combination with Bacteroides vulgatus or Fusobacterium necrophorum, or both. Each of the organisms had been isolated from swine with early lesions of the disease. Lesions were not found in 6 pigs inoculated with T hyodysenteriae alone, in 4 pigs given F necrophorum and T hyodysenteriae, or in 4 pigs given B vulgatus and F necrophorum. Lesions typical of swine dysentery developed in 8 pigs given B vulgatus, F necrophorum, and T hyodysenteriae as well as in 3 of 4 pigs given B vulgatus and T hyodysenteriae. In both of these groups, the inoculated bacteria were recovered from the colon, and T hyodysenteriae was demonstrated in the colonic crypts, epithelium, and lamina propria. The pathogenicity of the T hyodysenteriae was shown by the development of characteristic signs and lesions of swine dysentery in 12 of 14 naturally farrowed pigs inoculated with T hyodysenteriae alone.

Animals↗

Immunity to Lancefield's group E. streptococcus: flank inoculation of susceptible and immune swine.

Healthy swine flank inoculated with Lancefield's group E Streptococcus sp developed characteristic signs of streptococcal lymphadenitis of swine; however, abscesses were found in prefemoral lymph nodes rather than in cervical lymph nodes. After 6 months, swine (recovered from the cervical form of streptococcal lymphadenitis of swine caused by oral exposure to group E Streptococcus) were flank inoculated with group E Streptococcus sp. Only transitory signs of diseases developed and abscesses did not develop in the prefemoral lymph nodes.

Abscess↗

Cross protection in mice and swine immunized with live erysipelas vaccine to challenge exposure with strains of Erysipelothrix rhusiopathiae of various serotypes.

Mice and swine immunized subcutaneously with live vaccine prepared from acriflavine-fast attenuated Erysipelothrix rhusiopathiae, strain Koganei (serotype 2), were challenge exposed to virulent strains of E rhusiopathiae of various serotypes. Vaccinated mice did not die after challenge exposure to serotypes 1a, 1b, 2, 3, 5, 6, 7, 8, 9, 11, 12, 15, 16, 18, 19, 21, or N, but 20% to 30% mortality occurred in vaccinated mice challenge exposed to serotypes 10, 14, 20, or 22. Nonvaccinated control mice died after challenge exposure to all serotypes tested. Vaccinated swine challenge exposed to strain 14B (serotype 9) or strain 2179 (serotype 10) developed localized urticarial lesions at the site of intradermal exposure. Vaccinated swine challenge exposed to serotypes 1a, 1b, 2, 5, 8, 11, 12, 18, 19, or 21 did not have clinical signs of acute erysipelas. Nonvaccinated control swine developed acute generalized erysipelas or localized lesions at the site of intradermal exposure.

Animals↗

Transmission of swine dysentery by carrier pigs.

Swine dysentery (SD) was transmitted to healthy pigs by contact with experimentally-induced carrier pigs. Carrier pigs were produced by exposure of specific pathogen-free (SPF) swine to swine acutely affected with SD. When carrier pigs became acutely affected with SD, they were allowed to recover naturally or were treated with dimetridazole or ronidazole. Recovery was based on disappearance of clinical signs of SD. At a given time after recovery, normal SPF swine were housed with the carriers in a disinfected isolation unit to determine the ability of carriers to transmit SD. In each of 3 experiments, carriers that had recovered and remained asymptomatic for 11 to 25 days transmitted SD to contacts in 14 to 51 days. In 2 experiments, pathogenic Treponema hyodysenteriae was isolated from carriers 12 days before clinical SD was observed in contacts. Carriers which had recovered and remained asymptomatic for 70 and 90 days transmitted SD to contacts in 1 of 3 experiments. Treponema hyodysenteriae was isolated only from contacts with clinical signs of SD. In 4 experiments, carriers that had been treated with nitroimidazole compounds and subsequently recovered for 19 to 44 days failed to transmit SD. Culture of fecal samples on trypticase soy agar with 5% bovine blood and 400 microgram of spectinomycin/ml was helpful in predicting the carrier state, but phase contact contact microscopy of wet fecal smears was not.

Animals↗

Frequency of jowl abscesses in feeder and market swine exposed to group E streptococci as nursing pigs.

Weaning and isolation were evaluated as management methods in controlling streptococcic lymphadenitis of swine. Nursing pigs in 3 experiments were exposed to group E streptococcus (GES) by contact with their GES-inoculated dams or other GES-inoculated pen mates. The exposed pigs were either weaned and isolated when 5, 6, or 8 weeks old or left in contact with the inoculated swine until 14 to 32 weeks old when all were necropsied. Abscesses were not recognized in 13 weaned or 7 unweaned pigs necropsied at 14 to 15 weeks of age, but GES was isolated from mandibular lymph nodes of 4 of the 7 unweaned pigs. Abscesses were recognized in 1 of 49 unweaned and 4 of 53 weaned swine when examined at market weight (24 or 32 weeks of age). In the 3rd experiment, 10 of 11 inoculated sows, one of 33 unweaned, and 11 of 25 weaned swine had serum titers greater than 4 to GES in a microtitration agglutination test.

Abscess↗

Cerebral, coronary, and renal blood flows during hemorrhagic hypotension in anesthetized miniature swine.

The purpose of these studies was to measure cerebral, coronary, and renal blood flow in miniature swine during hemorrhagic hypotension. Blood flows (BF) were measured in 16 anesthetized, female, miniature swine (35-50 kg) with the radiolabeled microsphere technique using 15-microns spheres. The animals were exposed to a standard, stepwise hemorrhagic shock protocol which set mean aortic pressure at 65, 50, 35, and 20 mm Hg for 10-15 min at each stage. Heart rate and aortic and central venous pressures were measured throughout these studies. Arterial pCO2, pO2 pH, and Hct were measured at the time of BF measurements. BFs were measured under baseline conditions and during three of the four stages of hypotension in each animal. BF was measured in the following tissues: brain (13 samples), heart (120 samples), kidney (left and right), spleen, liver, muscle, and skin. Coronary BF was consistently decreased during hypotension. Endo/Epi flow ratios were also decreased; however, they remained greater than or equal to 1.0. Renal BF and BF to most other tissues showed graded decreases with hypotension. Cerebral BF did not change significantly at any level of hypotension. The maintenance of cerebral BF in swine at such low arterial pressures (22 mm Hg) may be related to the decrease in central venous pressure (-8 mm Hg), which results in a delta P of 30 mm Hg, and/or to the swine's carotid rete mirabile.

Animals↗

Brucellosis in wild swine: a serologic and bacteriologic survey in the southeastern United States and Hawaii.

Sera from wild swine from 20 populations in 11 southeastern states and Hawaii were tested for brucellosis by the card, tube, plate, buffered plate antigen, complement-fixation, and rivanol tests. Twenty-one of 352 (6.0%) swine were considered seropositive, and these animals originated from 7 populations in 5 states: Arkansas, Florida, Georgia, Louisiana, and South Carolina. Cultural examinations for Brucella suis also were made from subsamples of the swine tested serologically. Brucella suis biotype 1 was isolated from lymph nodes or seminal vesicles from wild swine in 2 areas in Florida and 1 in Louisiana.

Animals↗

Effects of aflatoxin consumption on the clinical course of swine dysentery.

Specific-pathogen-free pigs were examined for susceptibility to swine dysentery after daily treatment with aflatoxin B1 (0.07 to 0.14 mg/kg). In the pigs (group II) given both aflatoxin and Treponema hyodysenteriae, the incubation period before the onset of swine dysentery was shorter than that in pigs (group III) given T hyodysenteriae alone. Also, the number of days in which infected pigs showed signs of diarrhea and dysentery was greater in the group given aflatoxin and T hyodysenteriae than in the group given T hyodysenteriae alone. Four of 8 pigs given aflatoxin and T hyodysenteriae died during the experiment, whereas only 1 of 8 infected pigs in the other group died. Pigs in both groups, convalescent from swine dysentery, were immune to rechallenge exposure. Subclinical effects of aflatoxin consumption were evident at necropsy, but clinical signs and lesions of swine dysentery were not observed in group III pigs (given aflatoxin only).

Aflatoxins↗

Isolation of Mycoplasma flocculare from swine in the United States.

During 1977 to 1980, Mycoplasma flocculare was isolated from the lungs of 7 swine in the United States. Two of the swine were from a single Alabama herd, 1 was from Minnesota, 2 were from different Illinois herds, and 2 were from different Indiana herds. Three of the swine were from specific-pathogen-free herds and 4 were from conventional herds. The lungs from the 7 swine had gross lesions typical of mycoplasmal pneumonia. Two lungs yielded a mixture of M flocculare and M hyopneumoniae; the remaining 5 yielded M flocculare alone.

Animals↗

Prevention of gastric ulcers in swine by feeding of sodium polyacrylate.

Five experiments involving 169 swine were conducted to determine the effects of 0.1% to 0.5% sodium polyacrylate (PANa) in rations of growing swine on weight gain, feed conversion, and occurrence of esophagogastric lesions. Seemingly, PANa prevented swine from developing esophagogastric ulcers. Esophagogastric ulcers or erosions occurred in 42.9% of the swine fed a PANa-free diet, but occurred in only 9.8% of those fed a diet containing PANa. Addition of PANa (0.1% to 0.5%) to the growing ration increased weight gain and feed conversion. The anti-ulcer effect of PANa was discussed.

Acrylic Resins↗

Sources and reservoirs of Toxoplasma gondii infection on 47 swine farms in Illinois.

Field studies were conducted on 47 swine farms in Illinois during 1992 and 1993 to identify sources and reservoirs of Toxoplasma gondii infection. Blood samples were obtained from swine and from trapped wildlife. Serum antibodies to T. gondii were determined using the modified agglutination test, incorporating mercaptoethanol. Antibodies to T. gondii (titer > or = 25) were found in 97 of 4,252 (2.3%) finishing pigs, 395 of 2,617 (15.1%) sows, 267 of 391 (68.3%) cats, 126 of 188 (67.0%) raccoons, 7 of 18 (38.9%) skunks, 29 of 128 opossums (22.7%), 6 of 95 (6.3%) rats, 3 of 61 (4.9%) white-footed mice (Peromyscus sp.), and 26 of 1,243 (2.1%) house mice (Mus musculus). Brains and hearts of rodents trapped on the farm were bioassayed in mice for the presence of T. gondii. Toxoplasma gondii was recovered from tissues of 7 of 1,502 (0.5%) house mice, 2 of 67 (3.0%) white-footed mice, and 1 of 107 (0.9%) rats. Feces of 274 cats trapped on the farm and samples of feed, water, and soil were bioassayed in mice for the presence of T. gondii oocysts. Toxoplasma gondii was isolated from 2 of 491 (0.4%) feed samples, 1 of 79 (1.3%) soil samples, and 5 of 274 (1.8%) samples of cat feces. All mammalian species examined were reservoirs of T. gondii infection. All farms had evidence of T. gondii infection either by detection of antibodies in swine or other mammalian species, or by detection of oocysts, or by recovery from rodents by bioassay. The possibility of transmission of T. gondii to swine via consumption of rodents, feed, and soil was confirmed.

Animal Feed↗

Risk factors for transmission of Toxoplasma gondii on swine farms in Illinois.

Two epidemiologic studies of risk factors for transmission of Toxoplasma gondii to swine were conducted for farms in Illinois. The first study was a cross-sectional survey of swine farms from the state of Illinois pseudorabies testing program, in which farm owners or managers were interviewed by telephone regarding presence of risk factors for transmission of T. gondii on the farm. There were 123 farms surveyed that provided blood samples for at least 30 sows. The mean sow seroprevalence was 19.5% (median = 10.0%). Multiple regression analysis of the association of sow seroprevalence with outdoor housing of sows, cat access to sow areas, number of sows, open feed storage and water delivery, delayed removal of carcasses, and presence of rodents on the farm indicated that higher sow seroprevalence was associated with cat access to sows (P = 0.009) and fewer sows in the herd (P = 0.05). The second study was a field investigation of 47 swine farms (37 from the cross-sectional study). Data collection included obtaining blood samples from swine, cats, and rodents, and fecal samples from cats, heart and brain tissue from rodents, and feed, water, and soil samples for T. gondii examination. The risk of T. gondii transmission from cats and rodents to sows and finishing pigs was evaluated, taking into account housing conditions and herd size. Multiple regression analysis indicated that T. gondii seroprevalence in finishing pigs increased with more seropositive juvenile cats on the farm (P < 0.0001) and higher seroprevalence in house mice (P = 0.0023).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Effect of lincomycin on prevalence, duration, and quantity of Salmonella typhimurium excreted by swine.

Thirty-one swine (7.5 kg live wt) were fed diets which contained 0 or 110 mg of lincomycin/kg. These pigs were then inoculated with a nalidixic acid-resistant strain of Salmonella typhimurium and monitored for 56 days thereafter to determine (1) the quantity of S typhimurium shed in the feces, (2) the length of time the organism was shed, and (3) the number of swine which shed the organism for the 56-day period after exposure. Addition of lincomycin to diets did not alter these 3 criteria from those obtained in S typhimurium-exposed nontreated swine when results for nontreated and treated swine were compared. In addition, the sensitivity of the S typhimurium strain to 8 antibiotics, 1 nitrofuran, and 1 sulfonamide was not affected by in vivo exposure to lincomycin for 39 days. Lincomycin did not produce adverse effects in either inoculated or noninoculated pigs.

Animals↗

Influence of antibiotic-supplemented feed on occurrence and persistence of Salmonella typhimurium in experimentally infected swine.

The effect of chlortetracycline given at a concentration of 220.5 g/metric ton of feed and of a combination product which supplies chlortetracycline (110.2 g/metric ton), sulfamethazine (110.2 g/metric ton), and penicilin G (55.1 g/metric ton) on the occurrence and persistence of Salmonella typhimurium in experimentally infected swine was studied. Weanling pigs (av weight, 8.2 kg) were inoculated via the feed with 10(11) colony-forming units of S typhimurium 298-1NA. An equal number of nonexposed swine given identical treatment were used as controls. Infected pigs had increased temperatures (maximal av, 41 C) for the first 4 days after infection and severe diarrhea during the first 21 days. The use of chlortetracycline and a combination product at subtherapeutic concentrations in feed did not increase the Salmonella pool or prolong the carrier state in swine. A decrease in number of Salmonella shed from swine given chlortetracycline at the concentration of 220.5 g/metric ton was observed. Significant differences did not occur in Salmonella-related deaths or in emergence of multiple antibiotic-resistant Salmonella by antibiotic selection or R factor transfer. Zoonotic transmission of the infecting Salmonella to animal caretakers was not detected.

Administration, Oral↗

Characterization of the swine adipocyte A1 adenosine receptor using an optimized assay system.

The radioligand binding assay of A1 adenosine receptors in adipocyte crude plasma membrane from Yucatan miniature swine was optimized by evaluating 17 factors involved in the assay. Significant effects of CHAPS, adenosine deaminase, EDTA, pre-rinsing glass fiber filters and pH were found for the binding measurements. Using the optimized procedure, [3H]8-cyclopentyl-1,3-dipropylxanthine, ([3H]-DPCPX) binding to A1 adenosine receptors in swine subcutaneous adipocyte crude plasma membrane was measured; Bmax and Kd values were 479 +/- 77 fmol/mg protein and 0.87 +/- 0.10 nM, respectively. Values for mesenteric adipose tissue from sedentary swine and subcutaneous adipose tissue from exercise-trained swine were also measured.

Adenosine Deaminase↗

Relation between body temperature and dexmedetomidine-induced minimum alveolar concentration and respiratory changes in isoflurane-anesthetized miniature swine.

Dexmedetomidine (DEX), an alpha 2-receptor agonist, is the pharmacologically active d-isomer of medetomidine, a compound used as a sedative in veterinary medicine. Isoflurane anesthetic requirement (minimum alveolar concentration; MAC), rectal temperature, and cardiorespiratory variables were studied in chronically instrumented Yucatan miniature swine during DEX (20 micrograms/kg of body weight)-induced changes in body temperature. All studies were performed at room temperature of 22 C. The DEX was given as a 2-minute infusion into the left atrium. Each pig was studied twice. For protocol 1, the core temperature of the pigs was maintained at (mean +/- SD) 38.2 +/- 0.5 C by use of a thermostatically controlled water blanket and a heating lamp. For protocol 2, the core temperature was not externally manipulated and it decreased from 38.2 +/- 0.4 C to 32.2 +/- 1.2 C during the more than 3 hours of the protocol. Control isoflurane MAC was 1.66 +/- 0.2% and was 1.74 +/- 0.3% for protocols 1 and 2, respectively; DEX decreased MAC by 34 and 44%, respectively. For protocol 1, reduction in MAC after DEX administration returned by 50 and 80% at 84 and 138 minutes, respectively. If rectal temperature was not maintained (eg, allowed to decrease), MAC was reduced by 57% at the same time as the return to 80% in the swine with maintained body temperature. Respiratory rate and minute ventilation were significantly higher in swine with maintained temperature. The PaCO2 was lower and, accordingly, pH was higher in these swine. Blood pressure and heart rate were not affected by temperature changes.

Adrenergic alpha-Agonists↗