Hydrolytic activity of the perilymph in otosclerosis. A preliminary report.
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Data are reported from 323 otosclerotic ears in which primary surgery was carried out by using a large fenestra stapedectomy technique with fascial sealing of the window. In 129 ears the footplate was perforated with an argon laser. In the whole series, one ear became deaf (0.3%) as a result of granulomatous reaction in the operated area. Results in the argon laser group were slightly better than those in the group with mechanical footplate perforation and reconstruction with wire and fascia. Use of argon laser facilitates stapes surgery and, when used judiciously, it is a very helpful tool in all types of ear surgery.
Immunoglobulins G (IgG) and A (IgA) were found in plasma cells, osteocytes, chondrocytes and in connective tissue of active otosclerotic (= otospongiotic) lesions. The application of antibodies against paramyxovirus and rubella virus antigens reproducibly determined the expression of these antigens at different sites in the otosclerotic stapes, but also in the epithelial cells of the overlying middle ear mucosa. By using specific T-lymphocyte and B-lymphocyte markers, about 80% of the lymphocytes present in the otosclerotic footplate were revealed to be T-lymphocytes. There was neither deposition of immunoglobulins nor any expression of viral antigens in non-otosclerotic footplates which were investigated as controls.
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Acoustic resistance, reactance and phase angle were assessed for the two probe tone frequencies (220 and 660 Hz) on 29 otosclerotic middle-ear systems under accurately defined experimental conditions. Only otosclerotics with normal tympanic membranes were accepted in this statistical survey. The main purpose of these measurements was to provide numerical data for middle-ear modelling. If nevertheless a diagnostic parameter is sought from these data, the immittance values can be compared with data obtained on 30 normals under identical experimental conditions. The phase angle at 660 Hz seems the best choice, but the discrimination power is still poor.
A formula has been elaborated to estimate the resonance frequency of the middle ear from non-simultaneously recorded susceptance and conductance tympanograms at 220 and 660 Hz. The formula is tested on 30 normal and 29 otosclerotic ears. From these experimental results one can conclude that the middle-ear model (proposed by Lutman), from which the formula was deduced, is too crude to assess the resonance frequency. Nevertheless a ratio of phase angles functions at 220 and 660 Hz, introduced in that formula, is always qualitatively linked to the resonance frequency. Experimental data proved that this ratio can be used to distinguish tympanograms from otosclerotic ears with normal tympanic membrane from those obtained from normals. A statistical treatment is proposed to estimate the probability of false-positive or false-negative cases as a function of the value of that ratio.
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At the end of stapedioplasty, performed under local anesthesia, the surgeon usually tests the hearing function making questions to the patient turning his voice from a soft whisper to a loud tone. At the ENT Department, University of Parma Italy, a more precise method is employed in order to measure the air conduction threshold of the patient at the beginning and at the end of surgery. From April 1996 to October 1996, intraoperative pure-tone audiometry was performed in 36 patients who underwent stapedioplasty. A portable audiometer "Amplaid 161/C Amplifon" was used in the operating room. Air conduction thresholds were measured at 125-8000 Hz. Intraoperative pure-tone audiometry allows an instantaneous and a more accurate evaluation of the surgical functional results.
Otoesclerotic patients usually have neurosensorial hearing toss in addition to a predominantly conductive impairment, but there is no universally accepted explanation for this abnormality. The bone conduction threshold in tonal audiometry is accepted as a test of cochlear function. An early evaluation of damage to the cochlea arid acoustic nerve after surgery was made by studying 99 ears with BERA and latency/intensity curves to test cochlear function. We concluded that early damage was present. Postoperative cochlear damage was found in spite of successful surgery, but it was no greater than the damage present before surgery.
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