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At least 991 records · Page 55Linked to original sources
PubMed1995

Individual identification of flood victims by DNA polymorphisms and autopsy findings.

On September 23rd 1993 Genova was flooded by heavy rainstorms and 4 people disappeared, including an elderly couple. Four days later a partially skeletonized body was found floating near the coast. No visual identification was possible. Autopsy findings were consistent with the medical history of a possible victim. DNA was extracted from a muscle sample and compared to paraffin embedded prostatic gland fragment taken by surgery. A positive identification could be made. On October 11th the body of a decomposed and partially skeletonized female was found. The visual identification was also uncertain and no clinical records were available. A blood sample from the son was obtained for maternal identification by the polymerase chain reaction.

Aged↗
PubMed1998

Evaluation of six commercial systems for identification of medically important yeasts.

Six commercially available systems for the identification of yeasts were evaluated using 133 clinical isolates and four reference strains that had been previously identified by conventional methods and 19 recent clinical isolates that had been identified by the ID32C system (bioMérieux, France). The total identification rates (TIR) established for the total number of strains tested and the database identification rates (DBIR) established for the strains included in the respective manufacturer databases were both determined. After incubation for 4 h, the TIR and DBIR were 78% and 84%, respectively, for the RapID Yeast Plus system (Innovative Diagnostic Systems, USA). After incubation for 24 h, the TIR and DBIR were 32% and 32%, respectively, for the ID32C, 65% and 67% for the Auxacolor system (Sanofi Diagnostics Pasteur, France), 62% and 65% for the Fungichrom I system (International Microbio, France), 52% and 65% for the Fungifast I twin system (International Microbio), and 62% and 68% for the API Candida system (bioMérieux). The maximum TIR and DBIR (+/- 1%) obtained after incubation for 48 h were 86% and 88% for the Auxacolor, 85% and 89% for the Fungichrom I, 78% and 98% for the Fungifast I twin, and 82% and 91% for the API Candida. For the ID32C, the maximum TIR and DBIR were 98% and 98%, respectively, but these values were obtained only after 72 h of incubation. In addition, the six systems varied in their ease of use and readings. In conclusion, based on results obtained with 156 strains, the Auxacolor and Fungichrom systems seem the most appropriate for use in a clinical microbiology laboratory, due to their ease of use and reading, their rapidity, their cost per test, and their relatively high TIR results, which indicated acceptable performance with strains frequently isolated in our hospital. For a reference identification, the ID32C remains the sole system usable.

Evaluation Studies as Topic↗
PubMed1993

Evaluation of four commercial systems for identification of medically important yeasts.

Four commercially available systems for identification of yeasts were evaluated using 178 clinical isolates and seven reference strains previously identified by a conventional method. After 72 h of incubation, the rate of correct identification was 86.5% with API20C Aux, 86% with Auxacolor, 68% with Mycotube and 51.1% with Candifast. When considering only the reference strains included in the manufacturers' databases, the identification rate was 90%, 91%, 87% and 61.2% respectively. Although the results at 72 h obtained with API20C Aux and Auxacolor were similar, Auxacolor led to more rapid identification of the strains, 67.9% versus 14.6% being identified at 24 h and 80.9% versus 64% being identified at 48 h.

Evaluation Studies as Topic↗
PubMed1992

Comparison of the autoSCAN-W/A and Vitek Automicrobic systems for identification and susceptibility testing of bacteria.

The identification and susceptibility testing of bacteria by the autoSCAN-W/A rapid panels and Vitek Automicrobic systems were compared. A total of 291 clinical isolates, including 207 members of the Enterobacteriaceae, 41 nonfermentative gram-negative bacilli, 31 Staphylococcus aureus strains and 12 Enterococcus faecalis strains, were tested. autoSCAN-W/A and Vitek correctly identified 93% and 94% respectively of the Enterobacteriaceae, the identification in an additional 2% and 5% of strains respectively being reported as correct with a low probability. autoSCAN-W/A reported all identification results within 2 h after inoculation, whereas Vitek reported the identification results for the majority of gram-negative bacilli between 4 h and 6 h after inoculation. autoSCAN-W/A and Vitek reported 81% and 74% of MIC results respectively after 3-6 h incubation. The rates of very major susceptibility errors were 0.4% and 0.4% for autoSCAN-W/A and Vitek respectively, the rates of major errors 0.4% and 1% respectively, and the rates of minor errors 4% and 3% respectively. Six of 30 Pseudomonas aeruginosa strains failed to grow sufficiently for susceptibility testing in the autoSCAN-W/A system, and four Xanthomonas maltophilia strains had insufficient growth for susceptibility testing in both systems. The majority of results of susceptibility testing of nonfermentative gram-negative bacilli were reported more than 6 h after inoculation. Both systems were reliable and easy to operate, and gave accurate results for common clinical isolates.

Bacteria↗
PubMed1993

Proposed tests for the routine identification of Rochalimaea species.

A study was conducted to establish tests for the routine identification of Rochalimaea species. Strains used were reference strains of Rochalimaea vinsonii and Rochalimaea quintana, and a type strain and six human isolates of Rochalimaea henselae. Rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on MacConkey agar. Further testing of the organisms in a commercial identification system with the addition of hemin (100 micrograms/ml) to the medium revealed biochemical reactivity of the organisms not previously observed. The Voges-Proskauer reaction, tests for hydrolysis of hippurate and esculin, leucine arylamidase activity and the lactose test allowed identification and differentiation of the three species. Rochalimaea henselae was the only species with a positive lactose test and Rochalimaea quintana was the only species with a positive Voges-Proskauer reaction. Further studies are needed to confirm the validity of these tests for identification of Rochalimaea species.

Bacterial Typing Techniques↗
PubMed1983

ABAC identibiogramme: prototype of an automated system for identification of enterobacteria.

The ABAC Identibiogramme, an automated, computerized system for the identification and antimicrobial susceptibility testing of the Enterobacteriaceae has been developed recently. The biological basis of the system resides in lyophilized, highly discriminating media enclosed in wells of an automatically inoculated disposable cartridge. The introduction of a suitable specimen rehydrates and inoculates the media in the wells. After incubation for 16 to 18 h at 37 degrees C and the addition of only one reagent (Kovacs reagent), the media changes and the results obtained are interpreted by an automated optical system and a computer. The antibiogram is also used by the computer to confirm the biochemical identification of the organism. The identification accuracy of a prototype of the ABAC Identibiogramme system was compared with that of API 20 E and conventional methods using a total of 1,290 clinical isolates. The ABAC Identibiogramme correctly identified 96.8% of the organisms tested, misidentified 2.4% and failed to identify 0.8%. The results demonstrate the high reliability and good identification performance of the ABAC Identibiogramme in comparison to API 20 E and conventional methods.

Enterobacteriaceae↗
PubMed1986

Rapid identification of yeasts by semi-automated and conventional methods.

Five different methods for the identification of significant yeast from clinical specimens were compared for their reliability, rapidity and cost-effectiveness. Three commercial methods consisted of semi-automated Abbott's yeasts identification system using MS-2 (Abbott Laboratories, Diagnostic Division, Irving, Texas), API 20C (Analytab Products, Inc., Plainview, NY) and Uni-Yeast-Tek (Flow Labs, Inc., MacLean, VA). Two conventional methods included the modified dye-plur plate auxanographic and rapid tube assimilation method. The 242 coded clinical isolates used in this study included 20 species of Candida, Cryptococcus, Saccharomyces Geotrichum, Rhodotorula, Torulopsis and Trichosporon. The identification accuracies with all the systems ranges between 92.3% to 97.5%. Results were available with Abbott's MS-2 within 24 h, with rapid tube assimilation method in 6-48 h and in 72 h with other systems. Rapid tube assimilation and dye-pour-plate auxanographic methods were least expensive, with labour and material costing around $1.00 per identification, whereas the commercial system cost a little over $5.00.

Autoanalysis↗
PubMed1988

Nonlinear identification of stretch reflex dynamics.

The objective of this study was to use nonlinear identification techniques to study the dynamics of stretch reflexes in the human calf muscles (gastrocnemius-soleus). Stochastic perturbations of ankle position were applied while subjects maintained a constant, tonic contraction of gastrocnemius-soleus. Linear models of the relation between ankle velocity and the electromyographic (EMG) activity under these conditions typically accounted for less than 40% of the observed EMG variance. Nonlinear system identification techniques were then applied. The first- and second-order Wiener kernels were computed as the initial stage of this analysis. These did not provide an adequate description of system behavior; subsequent simulation studies showed that the major problem with the Wiener analysis was that the input spectrum was not adequately white. Nevertheless, the shape of the second-order Wiener kernel suggested that a Hammerstein structure consisting of a static nonlinearity followed by a dynamic linear system would be appropriate. Consequently, we used an iterative procedure for Hammerstein system identification to determine the form of the static nonlinearity and the associated linear dynamics. The resulting nonlinear model provided a much better description of the system's behavior than did the linear models (variance accounted for greater than 60%). Furthermore, they confirmed our previous empirical findings; the static nonlinearity closely resembled a half-wave rectifier while the dynamics were typified by a pure delay and a velocity filter. The application of nonlinear identification techniques thus produced a much improved, physically meaningful model of stretch reflex behavior.

Electromyography↗
PubMed1997

Identification and diagnostic evaluation of hearing impairments in early childhood in German-speaking infants.

The present study was devised to gather epidemiological information to aid in understanding the etiology, identification, and management of children with permanent hearing losses in Lower Saxony as a defined geographical area of Germany. All infants and preschool children were identified in the Department of Phoniatrics and Pediatric Audiology during a 1-year period (October 1994 until October 1995). Forty-four hearing-impaired children were identified, of whom 41 (93%) had sensorineural losses and 3 had pure conductive losses. Thirty-two children (73%) had bilateral hearing impairments. The causes of hearing loss were unknown in 17 children (39%). The median age of identification for the study group was 32 months, with a median age of 35.5 months when fitting hearing aids. Severity of hearing loss was inversely associated with age of identification. Prelingually deaf children had the worst comprehension levels of all children tested psychologically. Results were tabulated and special references to other studies were discussed. Our findings show again that the successful out-come of speech and language development depends on early identification and management of the hearing loss, and on continued assessment of the progress of habilitation. These require a basic assessment of the stage of language development (in perception and expression) as relevant rehabilitation measures. The next step is the treatment of existing linguistic deficits followed by retests at regular intervals.

Child, Preschool↗
PubMed1991

Practical identification of functional expansions of nonlinear systems submitted to non-Gaussian inputs.

Time-domain identification of nonlinear systems represented by functional expansions is considered. A general framework is defined for the analysis of three identification methods: the widely used cross-correlation method, Korenberg's method, and a suboptimal least-squares method based on a stochastic approximation algorithm. First, the major characteristics of the underlying estimation problem are pointed out. Then, the identification methods are interpreted as approximations to an optimal estimator, which helps gain insight into their internal functioning and to the investigation of their connections and differences. Examination of results previously published and of the simulations reported in this article indicate that stochastic approximation is an interesting alternative to other existing methods. Identification of a biological system stimulated by a non-Gaussian input confirms the practicality of this approach.

Binomial Distribution↗
PubMed1995

Evaluation of a PCR based method for identification of mycobacterial isolates.

In recent years an increasing number of mycobacterial species have been described as causing disease in humans. Identification of isolates to the species level is essential for evaluation of the significance of an isolate. Conventional methods for identification based on selective inhibitors of growth and biochemical reactions are slow. We have evaluated a novel method, PCR restriction enzyme pattern analysis (PRA), for identification of mycobacterial isolates. Fifty three cultures including a mixed culture of M. tuberculosis and M. avium and four mycobacterial cultures contaminated with rapidly growing organisms of other genera were studied. The method permits identification of most isolates within 1 to 2 days, including some contaminated cultures, and results were generally in accordance with those obtained by conventional methods.

Bacteriological Techniques↗
PubMed2004

Profile identification of disease-associated humoral antigens using AMIDA, a novel proteomics-based technology.

We describe AMIDA (autoantibody-mediated identification of antigens), a novel target identification technology based on the immunoprecipitation of disease-specific antigens by autologous serum antibodies followed by two-dimensional electrophoretic separation, and their identification via mass spectrometry. Twenty-seven potential carcinoma antigens were identified including proteins of hitherto unknown function. Validation of one of the identified antigens, cytokeratin 8, revealed its de novo expression in hyperplastic tissue, gradual overexpression with increasing malignancy, and ectopic localization on the cell surface. Furthermore, a strong prevalence of CK8-specific antibodies occurred in the serum of cancer patients already at early disease stages. In situ hybridization for one marker of unknown function, KIAA1273/TOB3, demonstrated its strong overexpression in head and neck carcinomas, thus making it a likely tumor antigen candidate. Eventually, AMIDA could foster significant improvements for the diagnosis and therapy of human diseases eliciting a humoral immune response, and allows for the rapid identification of new target molecules.

Allergy and Immunology↗
PubMed2000

[Intraoperative electromyographic identification of the recurrent laryngeal nerve].

BACKGROUND: Recurrent nerve palsy is the most important complication of thyroid surgery. Correct identification of the nerve during the surgical procedure is essential to avoid damage. METHOD: We evaluated the intraoperative electromyographic identification procedure of the recurrent laryngeal nerves in 77 consecutive patients. The operations performed were 63 subtotal resections, 6 hemithyroidectomies, 4 hemithyroidectomies in combination with subtotal resection of the opposite side and 4 thyroidectomies. RESULTS: Identification of the recurrent laryngeal nerve by electrophysiologic nerve stimulation was successful in 97.4%. Recurrent nerve palsy was not seen in postoperative laryngoscopic control. CONCLUSIONS: Intraoperative electromyography improves the identification rate of the recurrent laryngeal nerve and allows electrophysiologic verification of nerve function as a result of injury to the thyroid.

Adult↗
PubMed2004

Comparison of methods for the visual identification of prevalent vertebral fracture in osteoporosis.

The identification of vertebral fracture in osteoporosis is based mainly on the identification of abnormal variation in vertebral shape, but this can be misleading in the presence of a non-fracture deformity or normal variant of vertebral shape. Qualitative identification of vertebral fracture (Qual) is influenced by the subjectivity of the approach, and although more objective, the semiquantitative method (SQ) can be difficult to apply. In addition, there has been little independent evaluation of SQ in relation to other approaches. We aimed to evaluate a new algorithm-based approach for the qualitative identification of vertebral fracture (ABQ) and to compare it with SQ and Qual. Two radiologists reported spinal radiographs for 372 postmenopausal women using Qual (reader 1), and SQ and ABQ (reader 2). Non-fracture deformities and normal variants were also reported using Qual and ABQ. The prevalence of vertebral fracture by subjects was higher for SQ (24%) than for Qual (11%) and ABQ (7%). Agreement was poor between SQ and the other methods, and moderate between Qual and ABQ. Twenty-two women with vertebral fracture were agreed by all three methods, similar to the total identified by ABQ (25 women). Seventeen women diagnosed with fracture by Qual, had non-fracture deformity or normal variant (but no fracture) according to ABQ. Of the women with SQ fractures, 53% and 70% were identified negative for fracture but positive for non-fracture deformity or normal variant by ABQ and Qual. The main sources of discrepancy between SQ and the other methods were Scheuermann's disease, normal variation, and degenerative change accompanied by short anterior vertebral height. For all methods, bone mineral density (BMD) and BMD Z-scores were lower in women with vertebral fractures than in those with no fractures. Bone mineral density and BMD Z-scores were also lower at the lumbar spine and total body in women with vertebral fractures according to Qual and ABQ than they were for SQ, and were lower in women with SQ fractures agreed by Qual and ABQ, compared with those diagnosed negative for fracture by Qual and ABQ (p<0.01). We conclude that poor agreement between methods arises mainly from difficulties in differentiating true fracture from non-fracture deformity. Our new approach attempts to address this problem but requires further testing in a larger study population.

Aged↗
PubMed2003

Characterisation of quaternary mixtures by the apparent content curves method: identification of tocopherols in vegetable oils.

A procedure for identification of the compounds in quaternary mixtures has been developed. The proposed procedure is based on the apparent content curves method. From these curves and using the Q parameter, work conditions are selected and quaternary mixtures treated as "pseudoternary" mixtures.A simple strategy to test matrix effects at working wavelengths has been developed, identification limits established and following the identification table quaternary mixtures were easily characterised. The procedure has been applied to the identification of phenols in mixtures by UV-visible spectrophotometry and tocopherols in edible oils by fluorimetry. Results obtained for edible oils are in agreement with the composition obtained by use of the reference method.

Plant Oils↗
PubMed2004

Identification of the regulatory proteins in human pancreatic cancers treated with Trichostatin A by 2D-PAGE maps and multivariate statistical analysis.

In this paper, principal component analysis (PCA) is applied to a spot quantity dataset comprising 435 spots detected in 18 samples belonging to two different cell lines (Paca44 and T3M4) of control (untreated) and drug-treated pancreatic ductal carcinoma cells. The aim of the study was the identification of the differences occurring between the proteomic patterns of the two investigated cell lines and the evaluation of the effect of the drug Trichostatin A on the protein content of the cells. PCA turned out to be a successful tool for the identification of the classes of samples present in the dataset. Moreover, the loadings analysis allowed the identification of the differentially expressed spots, which characterise each group of samples. The treatment of both the cell lines with Trichostatin A therefore showed an appreciable effect on the proteomic pattern of the treated samples. Identification of some of the most relevant spots was also performed by mass spectrometry.

Analysis of Variance↗
PubMed1996

A new fast and fault-tolerant identification algorithm for spectral databases.

A new method for an automatic, computer and database driven identification of UV/VIS spectra is described. It is shown that an identification algorithm must consider the spectral differences as well as their common features. The described identification method allows identifications, even if the spectra are distorted or shifted.

Journal Article↗
PubMed2001

Penile lymphoscintigraphy for sentinel node identification.

Lymphoscintigraphy for sentinel node (SN) identification has been extensively validated in breast cancer and melanoma. The aim of this study was to evaluate the findings of lymphoscintigraphy for SN identification in carcinoma of the penis. Lymphoscintigraphy was performed in 74 consecutive patients (mean age 62.2 years, range 28-87 years) with clinically lymph node-negative squamous cell carcinoma of the penis (stage T2 or greater). Following local anaesthesia by xylocaine 10% spray, technetium-99m nanocolloid (mean dose 64.8 MBq, range 40-131 MBq) in a volume of 0.3-0.4 ml was injected intradermally around the tumour. Shortly after injection, a 20-min dynamic study was performed with a dual-head gamma camera; subsequently, static anterior and lateral images were obtained at 30 min and 2 h using simultaneous cobalt-57 flood source transmission scanning. 57Co-assisted skin marking defined SN location for gamma probe/blue dye-guided biopsy, which was performed the next day. The SN visualization rate was 97% (72/74). Lymphatic drainage was bilateral in 81% of the cases (58/72), exclusively to the left groin in 13% (9/72) and only to the right groin in 6%. Bilateral lymph node drainage was synchronous in 38% (22/58) and asynchronous in 62% (in 18 patients the initial route was the left groin, and in the other 18, the right groin). Visualization before 30 min occurred in 66 patients (93%), in 64 of them (88%) already during the dynamic study. A total of 173 SNs were visualized (85 in the right groin, 88 in the left groin). Pitfalls were caused by inguinal skin contamination during injection (four patients) and intracavernous administration (one patient). At surgery, a total of 161 SNs were identified and removed. Sixteen patients (22%) had a tumour-positive SN and underwent standard regional lymph node dissection subsequently. During follow-up (median 28 months, range 3-74 months), two patients with a negative SN developed lymph node metastases in the mapped basin. It is concluded that penile lymphoscintigraphy is a valid and well-tolerated method for lymphatic mapping and SN identification. Although bilateral early inguinal drainage is the most frequent pattern, late imaging is recommended principally in patients with initial unilateral drainage in order to exclude delayed lymph node filling in the contralateral groin. SN identification may lead to a more accurate staging and avoid extensive lymph node dissection in the majority of patients with penile carcinoma.

Adult↗