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At least 991 records · Page 55Linked to original sources
PubMed1989

[Persistence of serologic and allergic reactions in ewes after vaccination with the H.38 or Rev.1 vaccines in a brucellosis (Brucella melitensis) enzootic area].

In a flock of 2,000 ewes located in an endemic area of Brucella melitensis infection, subjected to systematic vaccination of lambs either with killed and adjuvanted B. melitensis H.38 vaccine from 1973 to 1978, or with living B. melitensis Rev.1 vaccine since 1979, reactions to allergic intrapalpebral test, complement fixation test and Rose Bengal plate test were recorded in small groups of animals vaccinated four, three, two or one years ago. Four years after H.38 vaccination, 39 ewes out of 40 (97%) remained positive at least to one of the three tests. After vaccination with strain Rev.1, 16% out of 153 ewes vaccinated one to four years before were positive. These results were discussed according to two aspects: 1) infectious or vaccinal origins of these positive reactions; 2) consequences on implement of test and slaughter procedures in a vaccinated flock: allergic or complement fixation tests would have led to eliminate 9% out of ewes vaccinated with the Rev.1 vaccine, but only 1% with the Rose Bengal plate test.

Animals↗
PubMed1998

Value of different serological tests in the diagnosis of bovine brucellosis in the Amazonian region.

The high prevalence of brucellosis is one of the most serious animal health problems affecting cattle and buffalo herds of the Amazonian region. Modern diagnostic methods are not used or not even known, and thus test results are not available in that region. Therefore, in this study a total of 878 selected sera were subjected to comparative examination by five different serological tests (buffered plate agglutination test, tube agglutination test, complement fixation test, indirect ELISA, competitive ELISA). Indirect ELISA gave the highest number of positive results, except in samples derived from the Marajó island, for which the competitive ELISA proved to be the most sensitive. The sensitivity of the classical tests (agglutination, complement fixation) was markedly lower than that of the two ELISAs. After vaccination of 2-month-old heifer calves with the B19 vaccine, all tests showed a 50-60% seropositivity, which disappeared within four months.

Agglutination Tests↗
PubMed1989

[Properties of phase I purified cellular antigen of Coxiella burnetii].

An attempt was made to purify phase I cell suspension of Coxiella burnetii used as an antigen in diagnostic serological tests. Homogenised suspension of chick embryos infected with phase I Henzerling and "Z" strains, after preliminary purification from host cell contaminants of chick embryos was subjected to consecutive centrifugation in sucrose/uropoline gradient and to continuous 20-45% uropoline gradient. The fractions obtained from uropoline gradient centrifugation were applied as phase I antigen C. burnetii in the following tests: complement fixation and microagglutination. Only fractions containing protein were serologically active. They proved to be of similar specificity and sensitivity as the antigens obtained by standard method. Moreover, it was found that after formalin treatment of C. burnetii cells no soluble antigens are liberated which could be detected by complement fixation test.

Agglutination Tests↗