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Deposition of radiocesium to the soil by stemflow, throughfall and leaf-fall from beech trees.

The amount of Chernobyl-derived 137Cs transferred to the soil by stemflow, throughfall (precipitation under the tree crown), and leaf-fall from three beeches was investigated as a function of time in the growing seasons of 1991 and 1992. Up to 70 Bq/week was deposited with the stemflow, mainly in dissolved form (< 0.45 micron) rather than in particulate form (> 0.45 micron). The ratio of dissolved radiocesium to particulate radiocesium was about 10 in the stemflow. It varied considerably with time, but since these variations followed the same pattern for all three trees, they indicated a common cause to be responsible for the fractionation of radiocesium (e.g. meterological conditions for bark weathering). A significant correlation was observed for the amount of dissolved 137Cs (in Bq) and the amount of stemflow (in liters). The 137Cs concentration in the stemflow (in Bq/1), however, decreased with increasing stemflow intensity (in Bq/week). For particulate radiocesium such correlations were not detected. Up to 5 Bq/m2 per week was deposited with the throughfall from the canopy, mainly in particulate form (ratio dissolved radiocesium to particulate radiocesium = 0.34). The mean total annual amounts of 137Cs deposited to the ground (dissolved+particulate) for the three trees were: stemflow: 1991 600 Bq; 1992 460 Bq; throughfall: 1991 and 1992 approximately 100 Bq/m2 each; leaffall: 1992 approximately 10 Bq/m2. The data indicate that at present a substantial amount of the radiocesium in the leaves derives already from root uptake.

Accidents↗

Brain cholinesterase activities of birds from forests sprayed with trichlorfon (Dylox) and carbaryl (Sevin-4-oil).

Brain cholinesterase activities were determined in birds from forests sprayed with Dylox(2) at 1.13 kg/hectare (1 lb/acre - active ingredient [a.i.]) or Sevin-4-oil(2) at 1.13 kg/hectare (1 lb/acre - a.i.) for up to 5 days postspray. Of ten bird species evaluated from the Dylox spray area, four species represented by six individuals had values which were depressed more than 2 standard deviations below the mean. Three of these activities (two species) were about 20% less than the mean. Of 12 species evaluated from the Sevin-4-oil spraying, three individuals representing three species had depressed values. One value was depressed greater than 20% below the mean. Half of the depressed activities were in canopy-dwelling birds collected on the day of spray.

Animals↗

Oxygen cost of breathing for assisted spontaneous breathing modes: investigation into three states of pulmonary function.

OBJECTIVE: We investigated the effects of continuous positive airway pressure (CPAP) and pressure support ventilation (PSV) on the oxygen cost of breathing (VO2resp) for different states of pulmonary function. Additionally VO2resp was measured during spontaneous breathing. DESIGN: This was done in a controlled and prospective study. Ventilatory modes were applied randomly. SETTING: Measurements were performed in a quiet room on volunteers (VOL) and inpatients treated for chronic obstructive pulmonary disease (COPD). Post-operative patients after aortocoronary bypass surgery (ACB) were studied on the cardio-thoracic intensive care unit just before and after extubation. PATIENTS: Healthy volunteers (n = 14), postoperative patients after aorto-coronary bypass surgery (n = 15) and patients with COPD (n = 9, xFEV1 47.7%) were the objects of study. INTERVENTIONS: Demand flow CPAP (5 mbar) and PSV (7 mbar, PEEP 5 mbar), using the Hamilton Veolar ventilator, were investigated in comparison to spontaneous breathing. MEASUREMENTS AND RESULTS: VO2 was measured by a Datex Deltatrac metabolic monitor. VO2resp was calculated by subtraction of total oxygen uptake (VO2tot) in controlled mode ventilation (CMV) from that in the respective spontaneous breathing mode. For VOL and COPD patients who were not intubated, a CPAP facemask connected to a short 7.5 mm tube was used as connection to the ventilator. Breathing spontaneously under a canopy system VOL showed a VO2resp of 4.5 +/- 4.0% compared to 9.2 +/- 3.5% for ACB and 15.4 +/- 7.7% for COPD. CPAP changed the VO2resp to 7.8 +/- 3.9%, 12.0 +/- 4.0% and 9.1 +/- 3.6% respectively. PSV reduced the VO2resp to 7.9 +/- 3.8% in ACB and 7.7 +/- 5.5% in COPD. CONCLUSIONS: This investigation confirms findings that postoperative patients have a mild increase in VO2resp. COPD exhibit the highest increase in VO2resp. Tracheal tubes, masks and CPAP on a demand flow apparatus increases VO2resp in volunteers and postoperative patients after cardiac surgery. The same amount of CPAP in contrary reduces VO2resp in patients with COPD. Pressure support ventilation can offset the additional VO2resp induced by CPAP but at the same level does not further reduce VO2resp in COPD patients.

Adult↗

Assessing habitat utilization by neotropical primates: a new approach.

This study aims to ascertain habitat utilization, in relation to forest structural variation, by a multi-species group of primates in tropical lowland rainforest in Southeast Peru during dry season. A new approach to assessing habitat utilization was used. Habitat variation was described by structural and indicator variables collected in quadrats along transects through a study area within Terra Firme and Floodplain forest. Variables were grouped into 'factors' accounting for most of the variation by means of a Principal Components Analysis (PCA). Presence or absence of the primates within the quadrats, assessed by repeat transect surveys, was taken to indicate habitat preferences. Discrimination between the habitat and forest structure in areas of primate presence as opposed to absence was carried out by means of Discriminant Function Analysis (DFA). This highlighted patterns in most utilized habitat. Vertical utilization of the forest was also assessed along with presence in bamboo and general activity on encounter. Suggestions of habitat preference and utilization are made for each of the six sympatric species studied, based on significantly discriminating habitat factors, vertical stratification on encounter and relationships with bamboo. Saguinus fuscicollis and Cebus moloch appeared as habitat generalists. Cebus apella, Saimiri sciureus, and Aotus spp., exhibited varying degrees of preference for habitat factors suggesting disturbed forest, Cebus albifrons was more generalistic but with a possible association with primary, naturally disturbed forest. C. apella was encountered in Terra Firme forest significantly more than in Floodplain. For S. sciureus, C. moloch, and C. apella, upper understory was the most utilized forest layer, for C. albifrons, middle canopy and for S. sciureus and Aotus spp., lower understory. Both positive and negative relationships with bamboo were highlighted. Significant positive relationships between Aotus spp., and bamboo suggest dense bamboo stands provide important daytime resting sites for this nocturnal species. C. moloch was also shown to utilize bamboo whereas S. fuscicollis appeared to actively avoid it. High levels of conformity with past studies in terms of habitat utilization patterns described for the species studied supports the conclusion that the methods used in this study provide an effective means of assessing primate habitat utilization within complex habitat.

Animals↗

Utilization of potatoes for life support systems in space. IV. Effect of CO2 enrichment.

Potatoes (Solanum tuberosum L.) cvs. Norland and Russet Burbank were grown in solid stands in separate controlled environment rooms at two CO2 levels, 365 micromoles mol-1 (ppm) and 1000 micromoles mol-1. Rooms were maintained under continuous fluorescent light (450 micromoles s-1 m-2 PPF), 16 C and 70% relative humidity. Norland plants were grown for 110 days and Russet Burbank plants for 126 days. CO2 assimilation rates (net photosynthetic rates) of exposed, upper canopy leaves were measured at weekly intervals beginning at 21-days-age for Norland and 28-days-age for Russet Burbank. Elevation of CO2 increased CO2 assimilation rates of Norland leaves by approximately 24%, but decreased rates of Russet Burbank leaves by approximately 12%. Assimilation rates of Norland leaves under the high CO2 decreased as plants matured so that their rates were similar to rates under the low CO2 levels after 70-days-age. Assimilation rates of Russet Burbank leaves under high CO2 remained depressed in comparison to low CO2 plants throughout the period of measurements. Yield data showed only marginal benefits from CO2 enrichment: tuber dry weight increased 2% for Norland and 12% for Russet Burbank, total plant dry weight was increased 6% for Norland and 4% for Russet Burbank. The best productivity obtained in this study (21.9 g tuber dry wt m-2 day-1 from Norland at 1000 micromoles mol-1 of CO2) indicates that the dietary energy needs of one human in space could be supplied from 34 m2 of potatoes.

Carbon Dioxide↗

Environmental fate and bioavailability of Agent Orange and its associated dioxin during the Vietnam War.

BACKGROUND: In 1996, the Committee on the Assessment of Wartime Exposure to Herbicides in Vietnam of the National Academy of Sciences' Institute of Medicine (IOM) issued a report on an exposure model for use in epidemiological studies of Vietnam veterans. This exposure model would consider troop locations based on military records; aerial spray mission data; estimated ground spraying activity; estimated exposure opportunity factors; military indications for herbicide use; and considerations of the composition and environmental fate of herbicides, including changes in the TCDD content of the herbicides over time, the persistence of TCDD and herbicides in the environment, and the degree of likely penetration of the herbicides into the ground. When the final report of the IOM Committee was released in October 2003, several components of the exposure model envisioned by the Committee were not addressed. These components included the environmental fate of the herbicides, including changes in the TCDD content over time, the persistence of TCDD and herbicides in the environment, and the degree of likely penetration of herbicides into the ground. This paper is intended to help investigators understand better the fate and transport of herbicides and TCDD from spray missions, particularly in performing epidemiological studies. METHODS: This paper reviews the published scientific literature related to the environmental fate of Agent Orange and the contaminant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and discusses how this affected the potential exposure to TCDD of ground troops in Vietnam. Specifically, the mechanisms of dissipation and degradation as they relate to environmental distribution and bioavailability are addressed. RESULTS: The evaluation of the spray systems used to disseminate herbicides in Vietnam showed that they were capable of highly precise applications both in terms of concentrations sprayed and area treated. Research on tropical forest canopies with leaf area indices (a measure of foliage density) from 2 to 5 indicated that the amount of herbicide and associated TCDD reaching the forest floor would have been between 1 and 6% of the total aerial spray. Studies of the properties of plant surface waxes of the cuticle layer suggested that Agent Orange, including the TCDD, would have dried (i.e., be absorbed into the wax layer of the plant cuticle) upon spraying within minutes and could not be physically dislodged. Studies of Agent Orange and the associated TCDD on both leaf and soil surface have demonstrated that photolysis by sunlight would have rapidly decreased the concentration of TCDD, and this process continued in shade. Studies of 'dislodgeable foliar residues' (DFR, the fraction of a substance that is available for cutaneous uptake from the plant leaves) showed that only 8% of the DFR was present 1 hr after application. This dropped to 1% of the total 24 hrs after application. Studies with human volunteers confirmed that after 2 hrs of saturated contact with bare skin, only 0.15-0.46% of 2,4,5-T, one of the phenoxy acetic acid compounds that was an active ingredient of Agent Orange, entered the body and was eliminated in the urine. CONCLUSIONS: The prospect of exposure to TCDD from Agent Orange in ground troops in Vietnam seems unlikely in light of the environmental dissipation of TCDD, little bioavailability, and the properties of the herbicides and circumstances of application that occurred. Photochemical degradation of TCDD and limited bioavailability of any residual TCDD present in soil or on vegetation suggest that dioxin concentrations in ground troops who served in Vietnam would have been small and indistinguishable from background levels even if they had been in recently treated areas. Laboratory and field data reported in the literature provide compelling evidence on the fate and dislodgeability of herbicide and TCDD in the environment. This evidence of the environmental fate and poor bioavailability of TCDD from Agent Orange is consistent with the observation of little or no exposure in the veterans who served in Vietnam. Appreciable accumulation of TCDD in veterans would have required repeated long-term direct skin contact of the type experienced by United States (US) Air Force RANCH HAND and US Army Chemical Corps personnel who handled or otherwise had direct contact with liquid herbicide, not from incidental exposure under field conditions where Agent Orange had been sprayed.

2,4,5-Trichlorophenoxyacetic Acid↗

Investigations of nitrogen fluxes and pools on a limestone site in the Alps.

In the North Tyrolean Limestone Alps a site was investigated over a four-year period (1998-2001) in order to assess the nitrogen saturation status, the nitrogen budget (quantification of the net uptake of nitrogen by the canopy and of the nitrogen mineralization, nitrogen uptake from roots and N2O emission rates, proof of the origin of nitrate in the soil water with stable isotope analyses), and the effects of the actual nitrogen input on ground water quality. The main goals were to quantify the nitrogen input rate, the nitrogen pools in above-ground and below-ground compartments, nitrogen turnover processes in the soil as well as the output into the groundwater and into the atmosphere. The findings are based on continuous and discontinuous field measurements as well as on model results. While nitrogen input exceeded the Critical Loads of the WHO (1995), nitrogen deficiency and nutrient imbalances were verified by needle analyses. The atmospheric input of inorganic nitrogen was higher than the nitrogen output in 50 cm soil depth. A tracer experiment with 15N helped to prove that not more than half of the applied nitrate could be discharged. This allows the conclusion that nitrogen is stored in the system and that the site cannot yet be said to be saturated with nitrogen. The same result was also obtained by modelling. In addition, it was proved that the nitrogen discharge did not stem from deposition but from processes within the system.

Altitude↗

Epidural anaesthesia and analgesia do not affect energy expenditure after major abdominal surgery.

Our objective was to determine the effect of perioperative epidural anaesthesia and analgesia on the increase in energy expenditure which accompanies major elective abdominal surgery in a prospective, randomized study. Eight patients undergoing elective resections of the colon and/or rectum received general anaesthesia alone (nitrous oxide, oxygen, and isoflurane, supplemented with intravenous fentanyl to a maximum of 10 micrograms.kg-1), and 12 patients received perioperative epidural anaesthesia and analgesia using lidocaine (carbonated lidocaine 2% with epinephrine 1:200,000, 20 ml over 30 min) and morphine (preservative-free morphine 0.10 mg.kg-1 after catheter insertion and 0.05 to 0.10 mg.kg-1 every 12 hr as needed until the morning following surgery) via a lower lumbar catheter in addition to general anaesthesia. Respiratory gas exchange was measured using a metabolic cart and canopy system early on the morning of surgery, six hours postoperatively, and on the first and second postoperative mornings. Parenteral analgesic administration (P < 0.001) and visual analogue pain scores (P < 0.05) were lower in the patients receiving epidural anaesthesia and time to first parenteral analgesia was longer (P < 0.005). Oxygen consumption, carbon dioxide production, and energy expenditure increased after surgery (all P < 0.001) but were very similar in the two groups (all P > or = 0.8) before and after surgery. Despite substantial effects on postoperative pain, we conclude that oxygen consumption and energy expenditure following major abdominal surgery are not diminished by perioperative epidural anaesthesia and analgesia.

Aged↗

Oral clonidine premedication decreases energy expenditure in human volunteers.

PURPOSE: Clonidine not only stops postoperative shivering and decreases oxygen consumption, but also decreases energy expenditure with or without a reduction in shivering during recovery from anaesthesia. It is important to see if clonidine decreases energy expenditure at rest since this may contribute to a postoperative decrease in energy expenditure. The authors tested the hypothesis that oral clonidine decreases energy expenditure at rest. METHODS: Twenty healthy male volunteers were randomly assigned to one of two groups. Ten volunteers received oral clonidine approximately 5 micrograms.kg-1 (clonidine group), while the remaining 10 volunteers received placebo (control group). Blood pressure, heart rate, body temperature at the tympanic membrane, sedation score graded from 1 (alert) to 5 (sleeping and difficult to be aroused by tactile stimulation) were measured before and at 30-min intervals for three hours after administration of clonidine or placebo. Measurements of energy expenditure and respiratory quotient were made with a head canopy system at one-minute intervals and averaged over 15 min before, and at 30, 60, 90, 120, and 180 min after administration of clonidine or placebo. RESULTS: Sedation score increased from 1 to 3 (median) after clonidine administration. Energy expenditure decreased from 1452 +/- 225 kcal.24hr-1 (mean +/- SD) at baseline to 1258 +/- 175 kcal.24hr-1 at 180 min after clonidine administration (P < 0.05). CONCLUSION: This study suggests that oral clonidine at a dose of 5 micrograms.kg-1 decreases energy expenditure at rest.

Administration, Oral↗

[Effect of capnoperitoneum on postoperative carbon dioxide homeostasis].

After laparoscopic cholecystectomy, carbon dioxide (CO2) must be exhaled after resorption from the abdominal cavity. There is controversy about the amount and relevance of postoperative CO2 resorption. Without continuous postoperative monitoring, after laparoscopic cholecystectomy a certain risk may consist in unnoticed hypercapnia due to CO2 resorption. Studies exist on the course of end-expiratory CO2 (Pe-CO2) alone over a longer postoperative period of time in extubated patients during spontaneous breathing. The goal of this prospective study was to investigate the amount of CO2 resorbed from the abdominal cavity in the postoperative period by means of CO2 metabolism. METHODS. After giving informed consent to the study, which was approved by the local ethics committee, 20 patients underwent laparoscopic cholecystectomy. All patients received general endotracheal anaesthesia. After induction, total IV anaesthesia was maintained using fentanyl, propofol, and atracurium. Patients were ventilated with oxygen in air (FiO2 0.4). The intra-abdominal pressure during the surgical procedure ranged from 12 to 14 mm Hg. Thirty minutes after releasing the capnoperitoneum (KP), CO2 elimination (VCO2), oxygen uptake (VO2), and respiratory quotient (RQ) were measured every minute for 1 h by indirect calorimetry using the metabolic monitor Deltatrac according to the principle of Canopy. Assuming an unchanged metabolism, the CO2 resorption (delta VCO2) at any given time (t) can be calculated from delta VCO2 (t) = VCO2 (t)-RQ(preop) VO2 (t). It was thus necessary to define the patient's metabolism on the day of operation. The first data were collected before surgery and after introduction of the arterial and venous cannulae for a 15-min period. Measuring point 0 was determined after exsufflation of the KP and emptying of the remaining CO2 via manual compression by the surgeon at the end of surgery. Patient's tracheas were extubated and metabolic monitoring started 30 min after release of the KP for 60 min. Simultaneously, a nasal side-stream capnometry probe was placed and the PeCO2 and respiratory frequency (RF) were obtained by the Capnomac Ultima (Datex) and registered every minute as well. Values were averaged over four periods of 15 min each. An arterial blood gas sample was drawn at the end of every 15-min period. Postoperative pain was scored by a visual analog scale and completed by a subjective index questionnaire on general well-being. All data were analysed by the Friedman or Wilcoxon test; P < 0.05 was considered significant. RESULTS. The findings do not indicate CO2 resorption in the postoperative period after laparoscopic cholecystectomy (Tables 2 and 3, Fig. 1). Arterial CO2 as well as PeCO2 were elevated postoperatively (45 mm Hg vs. 36 mm Hg intraoperatively), while VCO2 and VO2 were unchanged when compared to the preoperative measuring period. The postoperative RF was comparable to preoperative values. Calculated delta CO2 was lower than 10 ml/min and within accuracy of measurements. The post-operative pain index ranged between 3 and 4, and 3.75-15 mg piritramid was administered. All patients felt tired immediately after the operation, but scores improved slightly at the end of the 60-min period of metabolic monitoring. CONCLUSIONS. There is no significant resorption of CO2 from the abdominal cavity later than 30 min after releasing the KP. Up to this time, any CO2 remaining in the abdominal cavity after careful emptying by the surgeon has been resorbed and exhaled. An increased PeCO2 as late as 30 to 90 min postoperatively should rather be considered a consequence of residual anaesthetics and narcotics than of CO2 resorption.

Adult↗

Hot-blooded singers: endothermy facilitates crepuscular signaling in African platypleurine cicadas (Hemiptera: Cicadidae: Platypleura spp.).

The cicada genus Platypleura has a wide distribution across Africa and southern Asia. We describe endothermic thermoregulation in four South African species that show crepuscular signaling behavior. This is the first evidence of thermoregulation in platypleurine cicadas. Field measurements of body temperature ( T(b)) show that these animals regulate T(b) through endogenous heat production. Maximum T(b) measured was 22.1 degrees C above ambient temperature during calling activity at dusk. The mean T(b) during dusk activity did not differ from the mean T(b) during diurnal activity. A unique behavior for cicadas, a temperature-dependent telescoping pulsation of the abdomen, was observed in the laboratory during endogenous warm-up. This behavior is part of a unique method of heat generation in endothermic cicadas. Males generally call from trunks and branches within the canopy and appear to use endothermy even when the sun is available to elevate T(b). Endothermy may provide the cicadas with the advantage of decreasing predation and acoustic competition by permitting calling from perches that most complement their cryptic coloration patterns and that ectotherms cannot use due to thermal constraints. In addition, endothermy may permit calling activity during crepuscular hours when atmospheric conditions are optimal for acoustic communication and predation risks are minimal.

Abdomen↗

Ant-plant-herbivore interactions in the neotropical cerrado savanna.

The Brazilian cerrado savanna covers nearly 2 million km2 and has a high incidence on foliage of various liquid food sources such as extrafloral nectar and insect exudates. These liquid rewards generate intense ant activity on cerrado foliage, making ant-plant-herbivore interactions especially prevalent in this biome. We present data on the distribution and abundance of extrafloral nectaries in the woody flora of cerrado communities and in the flora of other habitats worldwide, and stress the relevance of liquid food sources (including hemipteran honeydew) for the ant fauna. Consumption by ants of plant and insect exudates significantly affects the activity of the associated herbivores of cerrado plant species, with varying impacts on the reproductive output of the plants. Experiments with an ant-plant-butterfly system unequivocally demonstrate that the behavior of both immature and adult lepidopterans is closely related to the use of a risky host plant, where intensive visitation by ants can have a severe impact on caterpillar survival. We discuss recent evidence suggesting that the occurrence of liquid rewards on leaves plays a key role in mediating the foraging ecology of foliage-dwelling ants, and that facultative ant-plant mutualisms are important in structuring the community of canopy arthropods. Ant-mediated effects on cerrado herbivore communities can be revealed by experiments performed on wide spatial scales, including many environmental factors such as soil fertility and vegetation structure. We also present some research questions that could be rewarding to investigate in this major neotropical savanna.

Animals↗

Ant patchiness: a spatially quantitative test in coffee agroecosystems.

Arboreal ants form patchy spatial patterns in tropical agroforest canopies. Such patchy distributions more likely occur in disturbed habitats associated with lower ant diversity and resource availability than in forests. Yet, few studies have quantitatively examined these patchy patterns to statistically test if ants are non-randomly distributed or at what scale. Coffee agroecosystems form a gradient of management intensification along which vegetative complexity and ant diversity decline. Using field studies and a spatially explicit randomization model, I investigated ant patchiness in coffee agroecosystems in Chiapas, Mexico varying in management intensity to examine if: (1) coffee intensification affects occurrence of numerically dominant ants, (2) numerical dominants form statistically distinguishable single-species patches in coffee plants, (3) shade trees play a role in patch location, and (4) patch formation or size varies with management intensity. Coffee intensification correlated with lower occurrence frequency of numerically dominant species generally and of one of four taxa examined. All dominant ant species formed patches but only Azteca instabilis was patchy around shade trees. Ant patchiness did vary somewhat with spatial scale and with strata (within the coffee layer vs around shade trees). Patchiness, however, did not vary with management intensity. These results provide quantitative evidence that numerically dominant ants are patchy within the coffee layer at different scales and that shade tree location, but not coffee management intensity, may play a role in the formation of patchy distributions.

Animals↗

Genetic analysis for drought resistance of rice at reproductive stage in field with different types of soil.

Drought resistance of rice is a complex trait and is mainly determined by mechanisms of drought avoidance and drought tolerance. The present study was conducted to characterize the genetic basis of drought resistance at reproductive stage in field by analyzing the QTLs for drought response index (DRI, normalized by potential yield and flowering time), relative yield, relative spikelet fertility, and four traits of plant water status and their relationships with root traits using a recombinant inbred population derived from a cross between an indica rice and upland rice. A total of 39 QTLs for these traits were detected with individual QTL explained 5.1-32.1% of phenotypic variation. Only two QTLs for plant water status were commonly detected in two environments, suggesting different mechanisms might exist in two types of soil conditions. DRI has no correlation with potential yield and flowering time under control, suggesting that it can be used as a good drought resistance index in field conditions. The co-location of QTLs for canopy temperature and delaying in flowering time suggested a usefulness of these two traits as indexes in drought resistance screening. Correlation and QTL congruence between root traits and putative drought tolerance traits revealed that drought avoidance (via thick and deep root traits) was the main genetic basis of drought resistance in sandy soil condition, while drought tolerance may play more role in the genetic basis of drought resistance in paddy soil condition. Therefore, both drought mechanisms and soil textures must be considered in the improvement of drought resistance at reproductive stage in rice.

Adaptation, Physiological↗

Pesticide deposition on coveralls during vineyard applications.

Deposition of pesticide on the clothing of the applicator was studied in a commercial vineyard using two different application technologies. A typical air-assisted sprayer with centrifugal fans delivered a concentrated spray. A tunnel or hooded sprayer was used at two carrier rates-high volume, low concentration versus low volume, high concentration-to apply Dithane M-45, an agricultural fungicide, at 3,375 g/ha on a light to medium density canopy. Deposition of pesticide was on the coveralls worn by the operator for all applications with a deposition range of 0.43 to 0.63 ng/cm2. The deposition on the clothing was higher for the air-assist sprayer than for the hooded sprayer. However, reducing the volume of water in the non-air assist hooded sprayer offered no advantage in terms of decreasing operator exposure. While the deposition of pesticide on the coveralls of the applicator was fairly uniformly distributed on the garment surface, the neck, shoulder, and upper right arm of our right-handed operator had the highest amount of pesticide deposit and the lower left quadrant of the garment had the lowest deposition. Results of this study indicate that vineyard applicator exposure can be reduced by use of the hooded non-air assisted sprayer and that extra protection is needed in the region of the neck, shoulder, and arm, and attention to the habits of the tractor driver is required.http://link.springer-ny. com/link/service/journals/00244/bibs/37n2p273.html

Agriculture↗

Influence of drying-rewetting frequency on soil bacterial community structure.

Soil drying and rewetting represents a common physiological stress for the microbial communities residing in surface soils. A drying-rewetting cycle may induce lysis in a significant proportion of the microbial biomass and, for a number of reasons, may directly or indirectly influence microbial community composition. Few studies have explicitly examined the role of drying-rewetting frequency in shaping soil microbial community structure. In this experiment, we manipulated soil water stress in the laboratory by exposing two different soil types to 0, 1, 2, 4, 6, 9, or 15 drying-rewetting cycles over a 2-month period. The two soils used for the experiment were both collected from the Sedgwick Ranch Natural Reserve in Santa Ynez, CA, one from an annual grassland, the other from underneath an oak canopy. The average soil moisture content over the course of the incubation was the same for all samples, compensating for the number of drying-rewetting cycles. At the end of the 2-month incubation we extracted DNA from soil samples and characterized the soil bacterial communities using the terminal restriction fragment length polymorphism (T-RFLP) method. We found that drying-rewetting regimes can influence bacterial community composition in oak but not in grass soils. The two soils have inherently different bacterial communities; only the bacteria residing in the oak soil, which are less frequently exposed to moisture stress in their natural environment, were significantly affected by drying-rewetting cycles. The community indices of taxonomic diversity and richness were relatively insensitive to drying-rewetting frequency. We hypothesize that drying-rewetting induced shifts in bacterial community composition may partly explain the changes in C mineralization rates that are commonly observed following exposure to numerous drying-rewetting cycles. Microbial community composition may influence soil processes, particularly in soils exposed to a significant level of environmental stress.

Classification↗

Detritus processing and microbial dynamics of an aquatic macrophyte and terrestrial leaf in a thermally constant, spring-fed stream.

Past studies of organic matter processing in stream ecosystems have focused on the fate of allochthonous terrestrial leaf detritus. In streams with a reduced canopy, submerged macrophytes may provide a significant source of organic matter to the microbial community and higher trophic levels. We compared mass loss patterns and microbial dynamics between a submerged macrophyte, Sagittaria platyphylla, and a deciduous leaf, Populus deltoides. Mass loss rates were higher for the submerged macrophyte, though exponential decay values indicated that both are "fast" decomposers. Bacterial abundance was not significantly different between plant types, but bacterial productivity was significantly higher in Sagittaria. Although fungal biomass was higher overall for Populus, it was not significantly different from that of Sagittaria until day 30. Relative to fungi, bacteria made up 4% and 7% of the peak microbial biomass on Populus and Sagittaria, respectively. Aquatic hyphomycete sporulation was detected only on Populus. These results suggest that in systems where submerged macrophytes are abundant, they can provide a carbon source quantitatively comparable to that of riparian leaf detritus, but that qualitative differences in leaf structural composition cause a shift toward a more significant role for bacterial decomposers.

Bacteria↗