Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “SWINE”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 973 records · Page 54Linked to original sources

[The eradication of African swine fever in Brazil, 1978-1984].

The African swine fever episode in Brazil was due to trade and tourism between Spain, Portugal and Brazil, at a time when outbreaks were on the rise in Europe. The eradication of the disease, the slaughter of pigs, the elimination of the carcasses and the isolation of affected farms were given wide media coverage, and had a major socio-economic impact. It was forbidden to raise pigs in garbage dumps or to give them feed considered hazardous. Analyses performed in Brazil as well as national and international investigations by researchers from reference laboratories concluded that the disease had spread from Rio de Janeiro to other states, as is stated in official reports. Following emergency measures, a control programme was implemented, leading to enhanced quality in the pig farming sector. The authors describe epidemiological surveillance of African swine fever, classical swine fever and related diseases, biosafety in swine farming, and the emergency action plan comprising animal health training for veterinarians and social workers. The results of the eradication programme were excellent, despite the controversy over compulsory sacrifice in a country with serious social problems. In 2004, Brazil was the fourth largest pork producer and exporter, with an output of 2.679 million tons and exports of 508,000 tons to international markets with very high standards.

African Swine Fever↗

[Quantitative study on copy numbers of porcine endogenous retroviruses in genome of Banna miniature swine inbred line].

OBJECTIVE: To investigate the copy numbers of porcine endogenous retroviruses in genome of Banna miniature swines for screening of donors in xenotransplantation with porcine transplants. METHODS: The genomic DNA of peripheral blood mononuclear cells(PBMCs) of 50 Banna miniature swines in 4 inbred sublines and 5 European Large White Pigs was extracted for the detection to pol, envA, envB, envC and envA/C recombinant in PERVs with real-time quantitative/semi-quantitative PCR and normal PCR. RESULTS: PCR products of pol, envA and envB of PERV could be obtained from all the Banna samples, and the mean amount of these products was 24.2+/-9.4,13.1+/-8.4,16.4+/-9.8 respectively and was significantly different among the inbred herds (P<0.01). The envC and envA/C were not amplified. CONCLUSION: The copy numbers of pol, envA and envB genes of PERV in porcine genome were significantly different among the inbred herds of Banna miniature swines, and envC and envA/C were absent in genome of Banna Miniature Swines.

Animals↗

Outbreaks of swine vesicular disease in japan: virus isolation and epizootiological survey.

Outbreaks of a vesicular disease occurred among pigs in Kanagawa and Ibaraki Prefectures in Japan in November, 1973. Another outbreak was observed in Aichi Prefecture in December. The clinical signs of the disease observed included fever and vesicular lesions on the coronary bands, bulbs of the heel and in the interdigital spaces. In some pigs, vesicular lesions were observed on the snout, tongue and skin overlying the legs and abdomen. All the vesicular samples produced cytopathic changes on cultures of primary swine kidney cells of PK-15 cells. Three isolates of cytopathic agents tested were identified as swine vesicular disease virus from their physicochemical properties and antigenicity. The virus strains isolated from vesicular epithelial samples obtained from Ibaraki, Kanagawa and Aichi Prefectures were designated as Japan/Ibaraki/1/73, Japan/Kanagawa/1/73 and Japan/Aichi/1/73 strain, respectively. An outbreak of the disease among pigs due to swine vesicular disease virus was confirmed by the serum neutralization test with serum samples collected from pigs on affected farms. Approximately 80% of the pigs housed in affected shed showed high levels of neutralizing antibody titers. This is the first to report an occurrence of swine vesicular disease among pigs in Japan.

Animals↗

A foot-and-mouth disease vaccine for swine.

An inactivated vaccine containing purified foot-and-mouth disease virus type O1, strain Brugge, emulsified with incomplete Freund's adjuvant was studied in swine. The antigen mass ranged from 0.02 to 416 mug in 0.25 ml of vaccine. At 90 days postinoculation (DPI) 33 to 100% of the swine which had been inoculated with 0.72% mug or larger amounts of antigen were protected against challenge. There was little protection at 182 DPI although the neutralizing titers obtained with 2.9, 34.6 and 416 mug doses of antigen were similar to those observed at 90 DPI. The 50% protective dose for swine was approximately 2.3 mug of antigen whether used in a freshly prepared state or after storage at 4 degrees C for 105 or 259 days. Significant protection was afforded when small volumes (0.1 and 0.5 ml) of vaccine were applied with a jet injector gun to the ear or neck of swine. Initial tissue reactions at the site of inoculation were minimal with these small doses of vaccine and generally disappeared ny 90 DPI.

Animals↗

Use of embryonating eggs for isolation of Campylobacter species from intestines of swine with proliferative enteritis.

Intestinal tissues from swine affected with proliferative enteritis were ground, filtered through a 0.65-micron pore membrane filter, diluted, and injected into 7-day-old embryonated hens' eggs via the yolk sac. At 2, 4, and 7 days later, yolk sac swab specimens taken from live embryos were cultured for Campylobacter species. Campylobacter hyointestinalis was recovered from eggs injected with tissues of swine with acute hemorrhagic proliferative enteritis at dilutions up to 10(-4). Campylobacter mucosalis was recovered from eggs injected with tissues of swine with chronic proliferative enteritis at dilutions up to 10(-6). Campylobacter coli was recovered from several specimens without lesions of proliferative enteritis and also from some specimens with lesions of proliferative enteritis. Two previously undescribed hemolytic Campylobacter species designed as hemolytic number 1 and hemolytic number 2 were recovered from normal and experimentally inoculated swine tissues. Few contaminating organisms grow in eggs and these were usually recovered at dilutions of 10(-2) or less. Recovery of Campylobacter species by use of these techniques was seldom successful in tissues stored at -70 C for more than 6 months.

Animals↗

Protection of cattle and swine against foot-and-mouth disease, using biosynthetic peptide vaccines.

A single dose of foot-and-mouth disease (FMD) virus protein 1 (VP1) peptide, expressed in Escherichia coli as a fusion protein with 190 amino acids (AA) of the LE' protein of the tryptophan operon of E coli, elicited an immune response in steers sufficient to withstand the challenge of exposure to animals with acute FMD. The 58-micrograms dose of viral peptide, composed of a segment of the VP1 from the A12 strain (A12) of FMD virus (FMDV; A12-32dimer) in a tandem repeat configuration of AA137 through 168 and emulsified with oil adjuvant, elicited a serologic response in cattle equivalent to that obtained using conventional whole virus vaccines. Two groups of swine were vaccinated, 1 with the A12-32dimer as used in cattle and 1 with AA131 through 157 from VP1 of the A24 strain (A24) of FMDV (A24-peptide), expressed in the same system as A12-32dimer, but as a single copy per molecule. In swine, the 58-micrograms dose of the A12-32dimer repeated at 28 days was an effective immunogen; all swine were protected against A12 and, in addition, the vaccine protected 50% of the swine against A24. The 29-micrograms dose of A24-peptide, administered according to the same schedule, elicited protection against A24 in 50% of the vaccinates and, in addition, protected 25% of those vaccinates against A12. The serologic response elicited by A12-32dimer against A24 virus was considerably greater than the response elicited by A24-peptide against A12 virus. The evidence of multiple immunogenic epitopes between AA131 and AA168 was evaluated.

Amino Acid Sequence↗

Economic analysis of an epizootic of pseudorabies and subsequent production following the institution of a vaccination program in a Pennsylvania swine herd.

The economic impact of pseudorabies was examined in a commercial swine herd. At the onset of clinical signs, a modified-live virus vaccine was administered to the sow herd and repeated at 3-month intervals. According to production data from the 320-sow farrow-to-feeder unit, preweaning mortality increased twofold, and subsequently, the number of pigs weaned per litter decreased by 19% (P less than 0.005) during the 5-week epizootic. Also, the number of pigs born alive decreased by 6% during the epizootic (P less than 0.05). No significant differences in production were observed between the 6-month periods before and after the epizootic. Actual cash flow analysis for the farm under isomarket conditions revealed a decreased net return of $2.40/inventoried sow/week, which was attributed to the disease during the epizootic, and a $0.46 decrease in net return/inventoried sow/week in the postepizootic period. Most seropositive herds have clinical signs less severe than those described in this herd, and the cost of eradication of the virus from a swine herd can be in excess of $200/inventoried sow. Thus, we believe that sufficient financial incentives are not available to all swine producers to ensure their enthusiastic cooperation in the effort to eradicate pseudorabies from the US swine population.

Animals↗

The postulated role of feeder swine in the perpetuation of the transmissible gastroenteritis virus.

Clinical, immunofluorescence and histopathological observations were found to be an efficient approach for the confirmation of the diagnosis of transmissible gastroenteritis in feeder swine. Two cases are reported to exemplify how feeder swine exposed to points of concentration such as holding areas, sales barns and auctions can play an important role in the epizootiology of transmissible gastroenteritis. A third field case is reported as an example of an outbreak of transmissible gastroenteritis beginning in feeder swine and then spreading to baby pigs on the farm. All baby pigs died that were born during the acute phase of the outbreak in the feeder swine. Baby pigs born shortly after the clinical signs had abated in the herd, and from sows that had been exposed orally to virulent transmissible gastroenteritis virus and vaccinated with a commercial transmissible gastroenteritis vaccine ten days before farrowing, survived. This was explained by a combination of a decrease in the amount of virus shed in the environment and the immunity induced in the sows. These observations of field outbreaks of transmissible gastroenteritis combined with recently reported experimental studies lend strong support to the hypothesis of a reservoir for transmissible gastroenteritis virus in feeder pigs. This reservoir would be based principally on the transmission of the virus on a continuous basis from the feces of recently infected pigs to susceptible pigs. Clinical signs of transmissible gastroenteritis in such pigs are difficult to recognize or absent and this contributes to the importance of the reservoir in the field.

Animal Husbandry↗

Experimental swine vesicular disease, pathology and immunofluorescence studies.

Two day old piglets were inoculated intravenously with 1 ml of swine vesicular disease virus UK-G 27-72 isolate. Using infectivity tests, immunofluorescent staining and gross and histopathological examination, pathogenesis of the infection was studied in tissue specimens collected daily from one through seven days postinoculation. Swine vesicular disease virus had a strong affinity for the epithelia of the tongue, snout, coronary band and lips, the myocardium and the lymphoid elements of the tonsil and the brain stem. The virus had the greatest affinity for the epithelium of the tongue. However, there was no evidence that the tongue was the initial replication site for swine vesicular disease virus. Prickle cells in the stratum spinosum appear to be the primary targets for the virus. The necrotic foci in the stratum spinosum appeared first, followed the next day by reticular degeneration and multilocular intraepidermal vesicular formation. In the digestive tract and most of the other visceral organs the short duration and sudden drop of the virus titres and the negative fluorescence and pathological findings suggest that these are not important sites for the replication of swine vesicular disease virus in this experiment. The virus was recovered from most of the central nervous tissue specimens. Although the piglets had significant central nervous system lesions, signs of impaired central nervous system function were not detected. However, subtle nervous signs could have been obscured by difficulties in locomotion resulting from severe lesions of the feet.

Animals↗

Epizootics of respiratory tract disease in swine in Belgium due to H3N2 influenza virus and experimental reproduction of disease.

In Belgium, influenza virus was isolated from swine in 22 epizootics of respiratory tract disease in swine during 1984. In 8 of the epizootics, H3N2 influenza virus, related to the A/Port Chalmers/1/73 strain, was isolated. Intratracheal inoculation of the isolates induced clinical signs. It was concluded that the A/Port Chalmers/1/73 strain was established in the Belgian swine population and was responsible for the epizootics of respiratory tract disease in swine.

Animals↗

[Preparation of Mycoplasma antigens and appropriate swine antisera].

An account is given in this paper of results obtained from development of methods for preparation of mycoplasma antigens and appropriate antisera from swine, as compared to normal swine sera. The exercise had been undertaken with the view to diagnosing mycoplasmosis in swine, on the basis of long-time complement fixation in microvolume. Tests were applied to 5 patterns of vaccination of swine, using antigens from mycoplasma. Benefits and drawbacks are discussed in some detail. Also described are methods for preparation, preservation, and storage of mycoplasma diagnostics which retain their suitability for the above diagnostic approach on the basis of 2-4 years of shelf life.

Animals↗

Experiments on the preparations and testing of associated vaccine against foot and mouth disease and vesicular disease in swine.

Tests for associated immunization of swine against Foot and Mouth Disease (FMD) and Vesicular Disease (SVD) of swine were carried out. As a result of this investigation, it was established that the prepared and tested inactivated oil vaccine is harmless and immunogenic in sensitive animals. In investigating the course of immunity, the presence of antibody against both antigens was demonstrated in vaccinated animals. All once-vaccinated animals were defended against the virus of SVD during challenge, and 75% of them were defended against FMD. After revaccination, all immunized swine were defended against infection with both viruses. The question of the quality of the associated vaccine and the possibilities of its massive use in industrial swine rearing was discussed.

Adjuvants, Immunologic↗

[Immunobiologic properties of swine rotavirus].

Immunobiological properties of swine rotaviruses were studied. A field isolate of this virus was markedly virulent for newborn colostrum-less piglets. An attenuated strain of swine rotavirus was obtained by serial passages in continuous cell cultures (MA-104 and SPEV). The virus of the 60th passage was nonvirulent for colostrum-less piglets although produced marked seroconversion. Naso-oral immunization of piglets with the attenuated strain prevented the manifestation of clinical disease upon challenge with the virulent swine rotavirus. Besides, the swine rotavirus of the 60th passage showed cross-protective activity against virulent human rotavirus in modelling human rotavirus infection in colostrum-less piglets.

Animals↗

Pancreatectomized swine as a model of diabetes mellitus.

The development of a model of diabetes mellitus using swine offers the potential for new investigations in the study of human diabetic complications. In particular, animal models for the study of accelerated atherosclerosis associated with diabetes are important and presently lacking. Swine were selected because they have a natural susceptibility to atherosclerosis and have plasma lipoprotein patterns which are close to those of humans. Diabetes mellitus was induced in nine miniature swine by total pancreatectomy. Following surgery, they were maintained on porcine derived insulin at doses predicated on blood glucose levels. Pancreatic enzymes were replaced by dietary supplementation. Eight of the nine pigs were pancreatectomized successfully and stabilized with insulin. After initial weight loss, the pancreatectomized pigs maintained growth rates comparable to controls. Hypoglycemia and bacterial infections were the major problems experienced. Post-operative survival ranged from 50 days to 455 days. Our study shows that swine can be pancreatectomized successfully and maintained as insulin dependent animals, presenting a realistic model for research on the complications of diabetes.

Animals↗

Comparative prevalence of four enterotoxin genes among Escherichia coli isolated from swine.

Presence of Escherichia coli enterotoxin genes LT (heat-labile enterotoxin), STaP (heat-stable enterotoxin a, porcine genotype), STaH (heat-stable enterotoxin a, human genotype), and STb (heat-stable enterotoxin b) among 874 swine isolates of E coli was determined, using DNA probes and the DNA colony hybridization technique. Of the 874 isolates evaluated, 45% hybridized with at least one of the enterotoxin gene probes and were designated as enterotoxigenic E coli (ETEC). Eighty-five percent of the ETEC were from pigs with enteric colibacillosis. The remaining 15% were from pigs with edema disease or various other diseases, and from healthy swine. Seventy-four percent of the ETEC hybridized with the STb probe, 52% with STaP, and 31% with LT; ETEC did not hybridize with the STaH probe. Most of the ETEC hybridized with more than one enterotoxin gene probe. Isolates that hybridized with the LT probe also hybridized with STb. The most prevalent gene combination was LT-STb. However, 35% of the ETEC from neonatal (less than or equal to 1 week old) swine with enteric colibacillosis were of the STaP-only genotype, and 33% of the ETEC from older swine with enteric colibacillosis were of the STb-only genotype.

Animals↗

[Eperythrozoonosis in swine--an overlooked disease?].

In the present work a review is given on the aetiology, clinical symptoms, prevalence and significance of the disease eperythrozoonosis in swine. Clinical outbreaks are characterized by fever, anemia and icterus. Clinical signs are most likely to be observed in young suckling piglets and in sows at the time of farrowing. In most cases infection with Eperythrozoon suis leads to a carrier state of symptomless, subclinical infection which, however, may be activated under conditions of stress. Eperythrozoonosis in swine has been reported from the USA, England, Federal Republic of Germany, Belgium and a few other countries. Due to the latent nature and the non-specific clinical symptoms of the disease it seems likely that the prevalence of E. suis in swine populations may be considerably greater than the number of clinical cases might lead one to suppose. So far, E. suis has not been diagnosed in Scandinavia, but it should not be excluded that eperythrozoonosis may exist as a neglected disease in Nordic swine herds as well.

Anaplasmataceae Infections↗

[Evaluation of the agglutination test (AT), complement fixation test (CFT) and the antiglobulin test (AGT) in the diagnosis of swine brucellosis. III. Basic studies].

In the examinations of the swine sera obtained from swines immunized s.c. with adjuvant Br.abortus S19 vaccine or Br.suis 1417 vaccine, it was found that agglutinins were present after 3 weeks, and C.F. antibodies or incomplete agglutinins normally after injections. Probably, in the first period of Brucella infection negative results of C.F.T. or AGT or both will be obtained. In the swine sera from Brucella free herds, agglutinins reacting with the Brucellognost antigen were present. The performance of mercaptoethanol test or C.F.T. lead in most cases to suitable diagnosis. In our conditions we have not obtained results which permit to classify AGT as a supplement test in serodiagnosis of swine brucellosis.

Agglutination Tests↗

Cross protection of mice and swine inoculated with culture filtrate of attenuated Erysipelothrix rhusiopathiae and challenge exposed to strains of various serovars.

Mice and swine inoculated subcutaneously with culture filtrate vaccine prepared from acriflavine-fast attenuated Erysipelothrix rhusiopathiae strain Koganei 65-0.15 (serovar 2), were challenge exposed to 20 pathogenic strains of E rhusiopathiae of 18 serovars and type N. Vaccinated mice survived after challenge exposure to serovars 1b, 2, 8 (strain Goda), and type N, but mortality occurred in vaccinated mice challenge exposed to other strains: 20% to 30% mortality in mice challenge exposed to serovars 1a, 11, 12, 15, 16, or 21; 40% to 50% mortality in mice challenge exposed to serovars 4, 5, 6, 7, or 8 (strain 911); and 60% to 80% mortality in mice challenge exposed to serovars 9, 10, 18, or 19. All vaccinated mice died after challenge exposure with strain 2553 (serovar 20). Non-vaccinated control mice died after challenge exposure to all strains. Of 2 vaccinated swine challenge exposed to strain 2553, 1 developed a local urticarial lesion at the site of intradermal exposure. Vaccinated swine challenge exposed to serovars 1a, 1b, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 16, 18, 19, 21, or type N did not have clinical signs of acute erysipelas. Nonvaccinated control swine developed acute generalized erysipelas or localized urticarial lesions at the site of intradermal exposure.

Agglutination Tests↗