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Effect of bentonite incorporated in a feed ration with tilmicosin in the prevention of induced Mycoplasma gallisepticum airsacculitis in broiler chickens.

A factorial arrangement of tilmicosin and bentonite was evaluated for efficacy in broiler chickens infected with Mycoplasma gallisepticum and correlated to tilmicosin recovery in a feed assay method. Tilmicosin at 300-500 g/ton prevented development of airsacculitis. The addition of 2% bentonite to the ration caused tilmicosin at 300 g/ton to be ineffective in controlling air-sac lesions, whereas 400 and 500 g/ton were moderately effective. Six percent bentonite rendered tilmicosin completely ineffective at all dose levels. There was a direct correlation between the percentage of bentonite in the feed rations, the percentage of the tilmicosin recovered in the assay procedure, and the increased incidence of air-sac lesions.

Air Sacs↗

Relating pharmacology to clinical practice: the pharmacologic basis of rational polypharmacy.

Many patients with epilepsy can be successfully treated with a single antiepileptic drug (monotherapy). However, in a substantial number of patients, monotherapy does not provide satisfactory seizure control, and the use of two or more drugs, or polypharmacy, is required. Selection of rational combination therapy requires consideration of the clinical antiepileptic or anticonvulsant effects, adverse effects, drug interactions, and relationship between effective and toxic drug levels. Mechanisms of action of each agent must also be studied. Optimal combination therapy often is achieved with drugs that have different mechanisms of action, relatively few adverse effects, high therapeutic indexes, and limited or no drug interactions. The goal of rational polypharmacy is to produce improved seizure control with minimal or no adverse effects.

Anticonvulsants↗

[Historical development of vaccines. Introduction: Hazards and rationality in the vaccinal approach].

The aim of this paper is to introduce the one hundred years of vaccination that has passed since Louis Pasteur first coined this generic term. According to the late Jonas Salk, vaccinology is a science encompassing all aspects of vaccine from its conception in the laboratory to its production by companies and its application and distribution in the field. In this historical survey I explore how vaccination never consisted of a simple and uniform application of a rational model, but rather diverged along various pathways, several of which were discarded in retrospect as being hazardous, and I analyse the ongoing interplay between rational and inventive thinking.

AIDS Vaccines↗

Combinatorial screening and rational optimization for hybridization to folded hepatitis C virus RNA of oligonucleotides with biological antisense activity.

We describe our initial application of a biochemical strategy, comprising combinatorial screening and rational optimization, which directly identifies oligonucleotides with maximum affinity (per unit length), specificity, and rates of hybridization to structurally preferred sites on folded RNA, to the problem of design of antisense oligonucleotides active against the hepatitis C virus (HCV). A fully randomized sequence DNA oligonucleotide (10-mer) library was equilibrated with each of two folded RNA fragments (200 and 370 nucleotides (nt)), together spanning the 5' 440 nt of an HCV transcript (by overlapping 130 nt), which were varied over a range of concentrations. The equilibrations were performed in solution under conditions determined to preserve RNA structure and to limit all RNA-DNA library oligonucleotide interactions to 1:1 stoichiometry. Subsequent Escherichia coli RNase H (endoribonuclease H: EC 3.1.26.4) cleavage analysis identified two preferred sites of highest affinity heteroduplex hybridization. The lengths and sequences of different substitute chemistry oligonucleotides complementary to these sites were rationally optimized using an iterative and quantitative analysis of binding affinity and specificity. Thus, DNA oligonucleotides that hybridized with the same affinity to the preferred sites in the folded RNA fragments found by screening as to short (< or = 25 nt) RNA complements were identified but were found to vary in length (10-18 nt) from site to site. Phosphorothioate (P=S) and 2'-fluoro (2'-F) uniformly substituted oligonucleotides also were found, which hybridized optimally to these sites, supporting the design of short (10-15-nt) and maximally specific oligonucleotides that are more nuclease-resistant (via P=S) and have higher affinity (via 2'-F) than DNA. Finally, the affinities of DNA and uniform 2'-F-, P=S-substituted 10-20-mer oligonucleotide complements for the best hybridization site, from HCV nt 355 to nt 364-374, closely corresponded to antisense mechanism inhibition activities in an in vitro translation assay and in a human cell-based HCV core protein expression assay, respectively. These results validate our strategy for the selection of hybridization-optimized and biologically active antisense oligonucleotides targeting HCV RNA and support the potential for utility in further applications.

Gene Expression Regulation, Viral↗

[Impact of a program of rational use of medicines on pharmaceutical prescription in a health area].

OBJECTIVES: To evaluate the impact of the introduction of a programme for rational use of medicines (PRUM) on the pharmacotherapeutic prescription profile of the primary care teams in the Ciudad Real health area. DESIGN: A longitudinal and prospective intervention study. The Area's total general medical prescription (January, May and August, 1994 and 1995) was used. SETTING: Ciudad Real Primary Care Area of INSALUD. PARTICIPANTS: 166 general practitioners, all the prescribing doctors in the area. INTERVENTION: Introduction of a programme of rational use of medicines. MEASUREMENTS AND RESULTS: Expenditure/inhabitant, prescriptions/inhabitant, expenditure/prescription, units of drugs of low therapeutic value (LTV), cost of calcitonins/pensioner and units of hypolipaemiants/100 pensioners were measured, in function of seven variables of the prescriber and six variables of the population group. Breaking down the prescriber's variables, least expenditure/inhabitant per month corresponded to doctors over 50, followed by temporary doctors, those not in paediatrics and those with their post in an urban centre. There was a positive correlation between expenditure/inhabitant per month and the number of prescriptions issued. CONCLUSIONS: 1. After the initial phase of PRUM, an increase in expenditure and improvement in the qualitative profile of prescription were noted. 2. Information taken from the study has proved to be an adequate planning and management tool.

Catchment Area, Health↗

[Rational support of combined operations in locally disseminated stomach cancer].

An analysis of results of 315 combined operations for gastric cancer with the T3 and T4 degrees of invasion has shown that the rational multicomponent surgical, anesthesiological and therapeutic maintenance of surgical interventions for gastric cancer is the main factor responsible for the outcome of the treatment. The proposed scheme of the multicomponent rational maintenance of the combined surgical interventions allowed to reduce frequency of cardiopulmonary complications to 18.2%, pyoseptic complications to 7% and postoperative lethality to 13.2%.

Adenocarcinoma↗

[Study on observation indexes of rational dosage of atropine in treatment of acute organophosphorus insecticides poisoning].

In order to obtain nursing observation indexes in treatment of acute organophosphorus insecticides poisoning (AOIP) with rational atropine dosage, three nursing observation indexes: abnormal secretion, body temperature and pulse were summarized with the theory obtained from a earlier study in our hospital. The rational atropine dosage for treatment of AOIP should be enough to antagonize the accumulation of acetylcholine but should not cause too much body feedback regulation. Regulate atropine dose by changes of these three indexes, 171 patients with AOIP (Group A) were treated and observed, and compared with 184 patients (Group B) treated with traditional observating method (atropinization index). The results showed that the atropine dose used in Group A was markedly less than that in Group B (P < 0.001), the medication duration was shortened (P < 0.001), the complication and fatality rate were decreased (both P < 0.05). It indicated that these observation indexes are scientific and reliable, practical and of spreading value.

Adult↗

Rational approach to combined carotid and ischaemic heart disease.

BACKGROUND: The management of patients with concomitant coronary and carotid artery disease remains a controversial subject. The aim of this review was to develop a rational plan for the management of such patients based on a review of the literature. METHOD AND RESULTS: A retrospective review was carried out of relevant papers derived from the Medline database from 1964 to 1996. CONCLUSION: The management of patients with concomitant coronary and carotid artery disease has not yet been put to the test in a properly designed and randomized multicentre trial. It is suggested that, until the results of such a trial are available, the rational approach to combined symptomatic disease is combined carotid endarterectomy and coronary artery bypass grafting (CABG). Combined surgery is also appropriate for patients with symptomatic carotid artery disease and significant but asymptomatic cardiac disease. At present there is inadequate evidence to promote carotid endarterectomy for asymptomatic disease in combination with CABG.

Carotid Artery Diseases↗

[Hygienic evaluation of food rations in the experiments with long-term isolation].

Hygienic evaluation of formulated food rations has been performed during 2 long-term studies (135 and 90 days) with participation of 6 male volunteers. The food rations provided for the energy and plastic demands of crewmembers, promoted the maintenance of working capacity and allowed to perform successfully the program of studies.

Adult↗

[Physiologic and hygienic bases of the rationalization of work schedules of several groups of miners in Arctic regions].

The article presents some data describing functional state in tunnelers and mining operators. That information should be considered in setting the rational schemes of work and rest during shifts in Transpolar conditions. The authors justified that heart rate is an expedient criterion of functional strain and useful for calculating the necessary rest time. Suggested by the authors, schemes of work and rest as well as facts and calculations are helpful to justify rational schemes of work and rest for other occupations.

Adult↗

[Assessment of the protein quota in the ration of patients with the nephrotic syndrome according to balance studies].

From data derived from equilibrium investigations in patients with the nephrotic syndrome a negative nitrous balance which indicates the presence of a chronic protein deficiency developing in the organism of these patients was revealed. Observations showed that an increased quota of protein in the ration of patients with the nephrotic syndrome is quite justifiable. The quantity of protein comprising 1.5--2 g per kg of the patient's body weight per day, of which 3/4 is protein of animal origin, is optimal for this category of patients. Possible improvement of the protein supply in patients with the nephrotic syndrome is largely determined also by the qualitative composition of the protein in the ration.

Dietary Proteins↗

[Rationalization of the use of preoperative thoracic radiography in obstetrics and gynecology].

INTRODUCTION: Rationalizing preoperative chest radiography remains a problem in our Country. Therefore, we tried to use preoperative chest films rationally in obstetrics and gynecology to assess their impact on anesthesia planning and patient management and their use in early postoperative complications. MATERIAL AND METHODS: We examined two groups of patients: group A consisted of 570 women (mean age: 31 years) scheduled to be submitted to cesarean section but with no preoperative chest radiography; group B consisted of 471 patients (homogeneous in age to group A patients) submitted to nononcologic gynecologic surgery and with a single-projection preoperative chest radiograph. Anesthesiologic assessment, preoperative biochemical tests and EKG were performed in all patients. All patients underwent abdominal surgery under general anesthesia. The first 24 postoperative hours were monitored for possible anesthesia-related complications. The anesthesiologist need of chest radiography based on clinical findings was investigated in group A patients, as well as the importance of chest film findings in possible anesthesia-related complications. RESULTS: Group A and group B were homogeneous by mean patient age and anesthesia duration; clinical findings never suggested the need of chest radiography in group A patients. Three cardiorespiratory complications occurred (two respiratory arrests in group A and a gas embolism in group B), but the (un)availability of chest film findings made no difference in treatment. DISCUSSION: The availability of the preoperative chest radiographs of a group of healthy women of 31 years mean age does not make any difference in anesthesia planning and type. In our series, the most severe cardiorespiratory complications were homogeneous in the two groups, which confirms their random character, and the (un)availability of preoperative chest film findings made no real difference, even though the lack of radiographic evidence made patient management more demanding for anesthesiologists.

Abdomen↗

Child health in America: toward a rational public policy.

Analysis of currently available data on mortality and morbidity indicates that the major organic illnesses of childhood, and their developmental consequences, are susceptible in part to the technical interventions of American medical science. Environmental forces, however, exert a powerful impact on the health of children in the United states, manifested both in the disproportionate toll of most organic diseases on poor and nonwhite populations and in such increasingly important symptoms of familial, social, and behavioral distress as child abuse, accidents, and childhood suicide. Review of the nature, quality, and distribution of child health services demonstrates a systemic inability to reach and treat the children most in need of them. A rational basis for child health policy includes: appropriate concepts of health, disease, and preventive and therapeutic intervention; a capacity to acknowledge, to measure, and to act on the familial and environmental, as well as the medical, sources of illness; an orientation to the developmental and social implications of good and poor child health; and a commitment to enable all children to receive health services. The data and this policy framework lead to these program recommendations: the channeling of resources into a more rational system which guarantees equity and access; a planning and program implementation mechanism which addresses the health needs of diverse local populations and which makes real the advocacy concept; a screening, evaluation, and surveillance methodology; a delivery system which both applies preventive and curative health technology and addresses basic life needs of children; and a coherent program for the training, assignment, and supervision of the several kinds of manpower which such a system would require.

Adolescent↗

Fish meal supplementation of chick starting rations containing cottonseed cake.

Between the second and the fifth week chicks were fed starting rations on the basis of corn/wheat with cottonseed cake up to 30% as sole plant protein and with 5 to 15% fish meal and 5 to 15% cottonseed cake supplemented at a ratio of 1:1. The crude protein levels tested in this way ranged between 11.7% (corn/wheat only) and 26.7% (15% fish feal, 15% cottonseed cake). The content of ME ranged between 2266 and 2920 kcal/kg, the total gossypol content between 0.051 and 0.307%. The most favourable results (considering increase and feed consumption) were obtained with a ration of 23.4% crude protein supplenented by 10% fish meal and 10% cottonseed cake. Higher contents of crude protein required a higher feed consumption, lower contents of crude proteins yielded less increase and higher feed consumption. Cottonseed cake additions only yielded medium increases but a high feed consumption.

Animal Feed↗

Rational-emotive behavior therapy and the formation of stimulus equivalence classes.

Stimulus equivalence is a behavioral approach to analyzing the "meaning" of stimulus sets and has an implication for clinical psychology. The formation of three-member (A --> B --> C) stimulus equivalence classes was used to investigate the effects of three different sets of sample and comparison stimuli on emergent behavior. The three stimulus sets were composed of Rational-Emotive Behavior Therapy (REBT)-related words, non-REBT emotionally charged words, and a third category of neutral words composed of flower labels. Sixty-two women and men participated in a modified matching-to-sample experiment. Using a mixed cross-over design, and controlling for serial order effects, participants received conditional training and emergent relationship training in the three stimulus set conditions. Results revealed a significant interaction between the formation of stimulus equivalence classes and stimulus meaning, indicating consistently biased responding in favor of reaching criterion responding more slowly for REBT-related and non-REBT emotionally charged words. Results were examined in the context of an analysis of the importance of stimulus meaning on behavior and the relation of stimulus meaning to behavioral and cognitive theories, with special appraisal given to the influence of fear-related discriminative stimuli on behavior.

Adult↗

Effects of rational-emotive therapy on psychophysiological and reported measures of test anxiety arousal.

Developed audio taped lectures, taped therapy session models, and homework assignments designed to reduce irrational beliefs associated with test anxiety within Ellis' rational-emotive therapy (RET) approach. The initial sample consisted of 148 university students. Comparisons with an attention placebo counseling program, which was established to be equally credible by a post-experiment inquiry and a no-treatment group found the RET Ss to show significantly lower skin conductance responses to a test anxiety visualization and lower reported anxiety on a questionnaire. However, skin conductance responses to an alternative test anxiety visualization did not show treatment effects.

Anxiety↗

The measurement of denial and rationalization in male alcoholics.

Two hundred male alcoholics were given 94 true-false items constructed to characterize alcoholic defensiveness, and a factor analysis revealed two strong factors. The first factor (Denial) was correlated with items that asserted an ability to control one's drinking, denied being an alcoholic or needing treatment, or minimized the consequences of alcohol abuse. Items that loaded the other factor (Rationalization) gave reasons, justifications, and excuses for drinking. Two derived scales were examined in a replication sample of 66 male alcoholics, and alpha coefficients (.84 and .85) from the first sample did not show undue shrinkage in the second (.86 and .77).

Adult↗