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At least 973 records · Page 54Linked to original sources
PubMed1999

Development of an agar lift-DNA/DNA hybridization technique for use in visualization of the spatial distribution of Eubacteria on soil surfaces.

While microbial growth is well-understood in pure culture systems, less is known about growth in intact soil systems. The objective of this work was to develop a technique to allow visualization of the two-dimensional spatial distribution of bacterial growth on a homogenous soil surface. This technique is a two-step process wherein an agar lift is taken and analyzed using a universal gene probe. An agar lift is comprised of a thin layer of soil that is removed from a soil surface using an agar slab. The agar is incubated to allow for microbial growth, after which, colonies are transferred to a membrane for conventional bacterial colony DNA/DNA hybridization analysis. In this study, a eubacterial specific probe was used to demonstrate that growing bacterial populations on soil surfaces could be visualized. Results show that microbial growth and distribution was nonuniform across the soil surface. Spot supplementation of the soil with benzoate or glucose resulted in a localized microbial growth response. Since only growing colonies are detected, this technique should facilitate a greater understanding of the microbial distribution and its response to substrate addition in more heterogenous soil systems.

Agar↗
PubMed1997

Box tilt and knee motions in manual lifting: two differential factors in expert and novice workers.

OBJECTIVE: It was the objective of this study to investigate the kinematic and kinetic effects of two specific handling factors that differentiate expert and novice workers, namely the level of knee flexion and box tilt. DESIGN: Seven inexperienced subjects were required to lift a 12-kg box in the sagittal plane using three different strategies: (1) reduced knee flexion and a backward box tilt (more typical of experts); (2) large knee flexion and a backward box tilt; (3) large knee flexion and no box tilt (more typical of novices). BACKGROUND: The lifting techniques of highly skilled workers differ substantially from those of novices but only limited information is available to compare their biomechanical differences. METHODS: The methods included dynamic segmental analyses to calculate the net moments at all body joints and a planar single-muscle equivalent to estimate compression loadings at L5/S1; total work and joint work distribution were calculated using the integration of joint power. The 3-D kinematic data were acquired with three video cameras and force data were obtained with one AMTI force platform which were synchronized with the film data. RESULTS: Box tilt reduced the load trajectory and loadings on the lower back and shoulders; a reduced knee flexion affected body posture and reduced mechanical work and loadings on all body joints. The combination of these two factors, box tilt and reduced knee flexion, showed added effects for almost all variables. CONCLUSIONS: These two factors proper to experts were, in this context, biomechanically more advantageous.

Journal Article↗
PubMed1996

Endoscopic forehead lift: technique and case presentations.

PURPOSE: The advent of the endoscopic forehead lift has provided an alternative to the conventional open approach. This article describes the basic technique, with some modifications, and reports three clinical cases. RESULTS: The subperiosteal forehead technique rejuvenates the upper third of the face with no scalp resection, minimal risks of hypesthesia, limited risk of alopecia, reduced tissue trauma, small camouflaged scars, less bleeding and edema, improved postoperative comfort and faster recovery compared with the standard open techniques. CONCLUSIONS: The endoscopic subperiosteal forehead lift is a useful technique for providing rejuvenation of the upper third of the face. It reduces or eliminates forehead rhytids by eliminating the reflex contracture of the frontalis and contributes to softening of the vertical glabellar rhytids. Longitudinal studies will be required to assess the effectiveness of this technique compared with open techniques.

Adult↗
PubMed1999

Periosteal and galeal adhesiveness after a forehead lift in a rodent model.

PURPOSE: The aim of this study was to determine the strength of periosteal and galeal adhesiveness at particular intervals after subperiosteal and subperiosteal-subgaleal forehead lifting in a rodent model. MATERIALS AND METHODS: Sixty mice underwent a subperiosteal or subperiosteal-subgaleal coronal forehead lift procedure. Necropic and histologic examination of the periosteal, galeal, and bone interface was performed on postoperative days 2, 4, 6, 8, and 10, and the strength of the elevated flap reattachment to underlying tissues was measured. RESULTS: Tension analysis in the early postoperative period showed a higher avulsive force was required to re-elevate subgaleal-subperiosteal flaps than subperiosteal flaps. However, subperiosteal flap adhesiveness appeared to increase rapidly between postoperative days 4 and 8. By day 10, the force required to re-elevate subperiosteal flaps was significantly higher than that required for subgaleal-subperiosteal flaps (P < or = .0001). Histologic analysis showed an inflammatory response at the periosteum-bone interface, which maximized at postoperative day 6. CONCLUSIONS: In the early postoperative period, subgaleal-subperiosteal flaps had higher adhesiveness than subperiosteal flaps. However, by postoperative day 10, subperiosteal forehead flaps were substantially more adherent in this rodent model.

Adhesiveness↗
PubMed1998

Jaw lift--a simple and effective method to open the airway in children.

Assessment of breathing during resuscitation of children is important. Misjudgement due to failure to open the airway may lead to mouth-to-mouth ventilation in unconscious children who have retained spontaneous breathing efforts, and might lead to completely ineffective ventilation with gastric distension. The efficiency of the standard head tilt-chin lift manoeuvre (HT-CL) and the jaw lift manoeuvre (JL) for opening of the airway in children was investigated. Fifty children between 1 and 9 years of age breathing spontaneously during deep anaesthesia were studied. Both manoeuvres were randomly performed in all children by nurse anaesthetists. The time for opening and the quality of the airway was determined by a blind folded anaesthesiologist listening to the breathing sounds. The standard HT-CL manoeuvre was insufficient in 12% of the children. JL was more effective than HT-CL in opening the airway in unconscious children who had retained attempts at spontaneous breathing. The JL manoeuvre may, therefore, be recommended in situations when the HT-CL manoeuvre is insufficient.

Age Factors↗
PubMed2000

Lift-off patterning of thin Au films on Si surfaces with atomic force microscopy

We studied a new lift-off process of thin Au film on silicon surfaces in nanometer-scale, combining anodic oxidation patterning with AFM, deposition of Au thin film on the patterned substrate and chemical etching processes of the Si oxide underneath the Au film. For Au films of thickness of 2-5 nm, the Au films on the Si oxide patterns were left unbroken and bent down to stick to Si surface after the removal of the oxide by the chemical etching. For an Au film of 1 nm in thickness, it was possible to lift-off the Au film on oxide patterns of the lines and dots in nanometer-scale using Si oxide as a sacrificial mask.

Journal Article↗
PubMedDate not supplied

Accidental hypothermia: a potential life-threatening hazard of automobile platform lifts.

The wheelchair/scooter of a patient with a relapsing-remitting form of multiple sclerosis was immobilized after inadvertent slippage of the rear wheels of his scooter off an automobile platform lift during travel. While waiting 45 min for the rescue squad, he developed mild hypothermia that responded to passive external rewarming techniques. This potentially life-threatening injury could have been prevented by using a platform lift equipped with full inboard and outboard roll-stop barriers on the platform.

Automobiles↗
PubMed2002

Total video endoscopic thyroidectomy via the anterior chest approach using the cervical region-lifting method.

Endoscopic surgery offers superior cosmetic results compared to open procedures and is strongly preferred by many patients, especially women. We performed total endoscopic thyroidectomy via the anterior chest approach using a neck skin-lifting technique in which the skin is lifted by a large number of hooks to create the working space. This method is quite flexible and can be modified based on the size of the space needed. The fine hooks leave no scar on the anterior neck, the skin incisions are small, and the scars are completely covered by patients' undergarments. Endoscopic thyroidectomy is suitable for benign thyroid nodules, but some malignant foci diagnosed by frozen section usually can be managed without conversion to an open procedure. Women under 45 years of age with nodules <2 cm who have no evidence of lymphatic spread or local invasion are ideal candidates for this procedure.

Adolescent↗
PubMed2002

Anterior chest wall approach for video-assisted thyroidectomy using a modified neck skin lifting method.

Neck endoscopic surgery is likely to prove less invasive and more cosmetically advantageous than conventional methods. The surgical procedure used for 42 consecutive patients undergoing video-assisted thyroidectomy by the anterior chest wall approach is described herein. A 3-4 cm incision was made at the anterior chest wall 2 cm below the clavicle on the tumor side. Two Kirshner wires connected to a winching device were horizontally inserted beneath the skin in order to lift the neck skin. Specially made retractors were passed through the chest wall wound to the lateral neck bilaterally. Strap muscle on the tumor side was retracted laterally and the thyroid tumor was retracted medially. A suitable working space was thus created using this modified neck lifting method without gas insufflation. All patients underwent video-assisted thyroidectomy utilizing an anterior chest wall approach without conversion to open thyroidectomy. Mean operative time from incision to removal of the tumor was 145 min. Mean tumor size was 4.1 cm (range: 2.0-7.5 cm). The advantage of our approach is that the scar below the clavicle is invisible under normal clothing. In addition, conventional instruments for open thyroidectomy can be used. In our method, the thyroid tumor and trachea are palpable and some parts of the operation can be conducted under direct visualization if the assistant retracts the anterior chest wall wound upward. Video-assisted thyroidectomy utilizing an anterior chest wall approach is cosmetically less invasive, particularly for women who are frequently nervous about operative scars on the neck.

Female↗
PubMed2003

Corneal wound healing after laser in situ keratomileusis flap lift and epithelial abrasion.

We report the clinical and confocal microscopic findings of wound-healing reactions after epithelium debridement and flap lift for the treatment of folds in a thin laser in situ keratomileusis (LASIK) flap. A 30-year-old woman had a flap lift with deepithelialization because of persistent striae after LASIK. The patient developed stage 2 diffuse lamellar keratitis (DLK) and dense haze. Confocal microscopy showed a loss of the most anterior keratocytes 6 days after flap relifting and deepithelialization. Subsequently, stromal repopulation with the appearance of spindle-shaped fibroblasts and activated keratocytes was detectable. Two months postoperatively, a maximum of haze tissue was found at the interface level and a myopic shift of -2.00 diopters was observed. Under treatment with topical steroids, the haze resolved and the spherical equivalent returned to baseline within 12 months.

Adult↗
PubMed1998

Peripheral radial incisions to treat inferior contact lens edge lift after penetrating keratoplasty for keratoconus.

A patient with marked corneal astigmatism and inferior contact edge lift after penetrating keratoplasty for keratoconus had corneal relaxing incisions (CRIs) on the donor button and radial incisions on the host cornea. Results were evaluated by computerized videokeratography and by refitting the contact lens. Two pairs of CRIs decreased astigmatism from 11.00 to 4.25 diopters but did not alleviate the contact lens edge lift, which was caused by excessive steepness in the keratoconic host cornea. After 6 radial incisions were made to flatten the inferior host cornea, the peripheral cornea flattened and the patient was successfully refitted with a contact lens.

Astigmatism↗
PubMedDate not supplied

Adaptive force generation for precision-grip lifting by a spectral timing model of the cerebellum.

We modeled adaptive generation of precision grip forces during object lifting. The model presented adjusts reactive and anticipatory grip forces to a level just above that needed to stabilize lifted objects in the hand. The model obeys principles of cerebellar structure and function by using slip sensations as error signals to adapt phasic motor commands to tonic force generators associated with output synergies controlling grip aperture. The learned phasic commands are weight- and texture-dependent. Simulations of the new circuit model reproduce key aspects of experimental observations of force application. Over learning trials, the onset of grip force buildup comes to lead the load force buildup, and the rate-of-rise of grip force, but not load force, scales inversely with the friction of the object.

Adaptation, Physiological↗
PubMed1997

The use of fibrin adhesive in sinus lift procedures.

Sinus lift bone grafting has expanded the use of dental implants in reconstructions of the atrophic maxilla. Potential problems include sinus membrane tear, which can lead to graft infection and early failure. Attempts at managing sinus membrane perforations are often limited by difficulty of access, as well as by the friability of the soft tissue lining the sinus. Various techniques have been proposed for managing such membrane tears. The use of fibrin adhesive, however, may present a potential solution in such situations. This article reports our experience with the use of fibrin adhesive in sinus lift procedures, as well as on its autologous preparation.

Bone Transplantation↗
PubMed2000

Detrimental neural control of precision grip lifts in children with ADHD.

The aim was to investigate the performance of children with attention-deficit-hyperactivity disorder (ADHD) in tasks involving motor-memory representations. A special grip object recorded forces generated by the fingertips during a precision grip-lift task. Common objects were lifted from a linear scale. Twenty-five boys with ADHD were evaluated and grouped according to the presence (ADHD+) or absence (ADHD) of movement dysfunction using the Movement Assessment Battery for Children (Henderson and Sugden 1992). Mean group ages were 11.4 years (range 9.0 to 11.0 years) and 11.7 years (9.0 to 15.6 years), respectively. They were compared to a control group of 25 age-matched boys, mean group age 11.8 years (range 9.0 to 13.0 years). Variability of motor performance was predominant in the ADHD+ group. Several of these participants presented a higher grip-force output during the gripping movement. They also had difficulties in adapting the motor output to target different weights, suggesting deficient anticipatory parameter control based on memory representations. The results suggest that in some children motor problems are due to detrimental neural control functions rather than core symptoms of ADHD.

Adolescent↗
PubMed1992

Dual-field and flow-programmed lift hyperlayer field-flow fractionation.

Field and flow programming and their combination, dual programming, are shown to extend the particle size range to which a single flow/hyperlayer field-flow fractionation (FFF) run is applicable to approximately 1-50 microns. The rationale for programming flow/hyperlayer FFF (or other forms of lift hyperlayer FFF) is to expand the diameter range of micron size particles that can be resolved in a single run. By contrast, the reason for programming normal-mode FFF, the only kind of programming previously realized in FFF, is to reduce the analysis time of submicron particle samples of considerable size variability. These differences are explained in detail in relationship to the basic mechanisms governing retention in normal, steric, and lift hyperlayer FFF. Experiments are described in which field, flow, and dual programming are used to expand the accessible diameter range of flow/hyperlayer FFF. An example is shown in which 11 sizes of latex microspheres in the 2-48-microns diameter range are separated by dual programming in 11 min.

Chemical Fractionation↗
PubMed2002

Screening for disulfide bonds in proteins by MALDI in-source decay and LIFT-TOF/TOF-MS.

An automated screening method is presented that uses MALDI in-source decay (MALDI-ISD) of disulfide bonds for identification of disulfide-linked peptides in MALDI mass spectra. Peptides released by ISD of a disulfide bond can be detected at an m/z ratio that corresponds to the singly protonated peptide with a reduced cysteine residue. Therefore, screening of peak lists for signal patterns that fulfill the equation, m/z (peak A) + m/z (peak B) - m/z (H2 + H+) = m/z (peak C), facilitated identification of putative ISD fragments of disulfide-linked peptides (peaks A and B) and their precursors (peak C). Signals (peak C) from putatively disulfide-linked peptides were subjected to LIFT-TOF/TOF-MS to confirm the existence of a disulfide bond. Using this method, we identified all 4 disulfide bonds in RNAseA and 8 two-disulfide clusters comprising 16 out of the 17 disulfide bonds in BSA. The presented screening method accelerated the identification of disulfide bonds in RNAseA and BSA, because the number of MS/MS spectra to be acquired was reduced by 1 order of magnitude. Less than 5% of the signals selected for LIFT-TOF/TOF-MS did not correspond to disulfide-linked peptides. Furthermore, the number of possible assignments for disulfide-linked peptides was reduced by 2-3 orders of magnitude, because knowledge of the mechanism of disulfide bond fragmentation by ISD permitted use of stricter rules for the interpretation of mass spectra. Therefore, interpretation of MS/ MS spectra of disulfide-linked peptides was considerably simplified in comparison to conventional approaches.

Algorithms↗
PubMedDate not supplied

Production of human alkaline phosphatase, a secreted, glycosylated protein, from a baculovirus expression system and the attachment-dependent cell line Trichoplusia ni BTI-Tn 5B1-4 using a split-flow, air-lift bioreactor.

A split-flow, air-lift bioreactor for the cultivation of insect cells to produce recombinant protein is described. It can be used advantageously with attached cell systems. This bioreactor incorporates two sections: a rise and a downcomer. Trichoplusia ni BTI-Tn 5B1-4 cells are grown on a support material of glass beads or microcarriers placed in the downcomer. This cell line is more productive than other commonly used insect cell lines, but it has the disadvantage of being difficult to use at large volumes since it is not easily adaptable to suspension culture. Adequate oxygen demand is supplied by sparging without direct exposure of cells to air bubbles. Nutrients are supplied convectively to the attached cells on support material as the fluid flows through the downcomer. The split-flow, air-lift bioreactor appears to be suitable for insect cell culture and is potentially scalable. It can provide a high surface-to-volume ratio and can be operated in batch or continuous mode. A lab-scale prototype bioreactor has been constructed and tested for the production of a secreted, glycosylated recombinant protein (human alkaline phosphatase) or seAP using an Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) vector. With a ratio of riser cross-sectional area to downcomer cross-sectional area of 1, an aspect ratio of 4.4, an air-flow rate of 54 mL/min in the riser, and a bed of 2400 3-min nonporous glass beads, 10.7 micrograms/mL of seAP was produced using an MOI (multiplicity of infection or the ratio of plaque-forming units to cells) of 10.(ABSTRACT TRUNCATED AT 250 WORDS)

Alkaline Phosphatase↗
PubMedDate not supplied

Sucrose conversion by immobilized invertase in a multiple air-lift loop bioreactor.

A new bioreactor series within one vessel, the multiple air-lift loop reactor (MAL), is introduced. In the MAL, a series of air-lift loop reactors is incorporated into one vessel. From residence time distribution studies, it was shown that the three-compartment MAL behaves as a series of three ideal mixers. A continuously operated MAL, containing immobilized invertase as a model biocatalyst, was evaluated. The advantage of approaching plug flow by using a bioreactor cascade could be shown by comparing substrate conversion in the three-compartment MAL to that in a single vessel at the same overall dilution rate. This was done for two sets of experimental conditions, which were chosen by using a previously developed model. Intrinsic kinetic parameters of the immobilized enzyme, needed for the model calculations, were determined experimentally. Model calculations gave good approximations of the results. The model incorporates external mass-transfer resistance and diffusion and reaction in the biocatalyst beads.

Biotechnology↗