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Bounding the risk of crew loss following orbital debris penetration of the International Space Station at assembly stages 1J and 1E.

Orbital debris impacts on the International Space Station occur frequently. To date, none of the impacting particles has been large enough to penetrate manned pressurized volumes. We used the Manned Spacecraft Crew Survivability code to evaluate the risk to crew of penetrations of pressurized modules at two assembly stages: after Flight 1J, when the pressurized elements of Kibo, the Japanese Experiment Module, are present, and after Flight 1E, when the European Columbus Module is present. Our code is a Monte-Carlo simulation of impacts on the Station that considers several potential event types that could lead to crew loss. Among the statistics tabulated by the program is the probability of death of one or more crew members in the event of a penetration, expressed as the risk factor, R. This risk factor is dependent on details of crew operations during both ordinary circumstances and decompression emergencies, as well as on details of internal module configurations. We conducted trade studies considering these procedure and configuration details to determine the bounds on R at the 1J and 1E stages in the assembly sequence. Here we compare the R-factor bounds, and procedures could that reduce R at these stages.

Accident Prevention↗

The minimal cost of life in space.

The cost of keeping people alive in space is assessed from a theoretical viewpoint and using two actual designs for plant growth systems. While life support is theoretically not very demanding, our ability to implement life support is well below theoretical limits. A theoretical limit has been calculated from requirements and the state of the art for plant growth has been calculated using data from the BIO-Plex PDR and from the Cornell CEA prototype system. The very low efficiency of our current approaches results in a high mission impact, though we can still see how to get a significant reduction in cost of food when compared to supplying it from Earth. Seeing the distribution of costs should allow us to improve our current designs.

Agriculture↗

The FLUKA code for space applications: recent developments.

The FLUKA Monte Carlo transport code is widely used for fundamental research, radioprotection and dosimetry, hybrid nuclear energy system and cosmic ray calculations. The validity of its physical models has been benchmarked against a variety of experimental data over a wide range of energies, ranging from accelerator data to cosmic ray showers in the earth atmosphere. The code is presently undergoing several developments in order to better fit the needs of space applications. The generation of particle spectra according to up-to-date cosmic ray data as well as the effect of the solar and geomagnetic modulation have been implemented and already successfully applied to a variety of problems. The implementation of suitable models for heavy ion nuclear interactions has reached an operational stage. At medium/high energy FLUKA is using the DPMJET model. The major task of incorporating heavy ion interactions from a few GeV/n down to the threshold for inelastic collisions is also progressing and promising results have been obtained using a modified version of the RQMD-2.4 code. This interim solution is now fully operational, while waiting for the development of new models based on the FLUKA hadron-nucleus interaction code, a newly developed QMD code, and the implementation of the Boltzmann master equation theory for low energy ion interactions.

Computer Simulation↗

Microbial life in permafrost.

Hydrogenotrophic and acetoclastic methanogenesis was measured at temperatures between 5 and -16.5 degrees C with H14CO3- and 14CH3CO2- as substrates in Siberian permafrost soils. The rate of methane formation was reduced approximately 2-fold over the temperature range from 5 to -1.8 degrees C. For the most active sample "a" temperature dependence of CH4, production at negative temperatures was approximately a 100-fold reduction for a range of -1.8 to -16.5 degrees C for both substrates. According to the Arrhenius equation, the activation energy of methane generation from bicarbonate and acetate for the temperature interval -5 to -16.5 degrees C was reduced by a factor of 3 and 1.5, respectively, in comparison with the temperatures above zero. In the experiments we tested the geological time series, showing the ability of microorganisms to carry out redox reactions after thousands to millions years of existence in permafrost. From the Climate Change point of view, it is important that the recovered organisms are quickly involved anew in present-day ecological processes after instances of permafrost thawing, and may be vital in nutrient recycling and in the production and consumption of greenhouse gases over a large portion of the Earth's surface. From an exobiological point of view, the terrestrial permafrost, inhabited by cold adapted microbes and protecting the cells against unfavorable conditions, can be considered as an extraterrestrial model. The methanogenic bacteria and their metabolic end-products found in the Earth's permafrost provide a range of analogues that could be used in the search for possible ecosystems and potential inhabitants on extraterrestrial cryogenic bodies free of oxygen.

Acetates↗

Cellular parameters for track structure modeling of radiation hazard in space.

Based on irradiation with 45 MeV/u N and B ions and with Co-60 gamma rays, cellular parameters of Katz's track structure model have been fitted for the survival of V79-379A Chinese hamster lung fibroblasts. Cellular parameters representing neoplastic transformations in C3H10T/1/2 cells after their irradiation with heavy ion beams, taken from earlier work, were also used to model the radiation hazard in deep space, following the system for evaluating, summing and reporting occupational exposures proposed in 1967 by a subcommittee of NCRP. We have performed model calculations of the number of transformations in surviving cells, after a given fluence of heavy charged particles of initial energy 500 MeV/u, penetrating thick layers of cells. We take the product of cell transformation and survival probabilities, calculated along the path lengths of charged particles using cellular survival and transformation parameters, to represent a quantity proportional to the "radiation risk factor" discussed in the NCRP document. The "synergistic" effect of simultaneous charged particle transfers is accounted for by the "track overlap" mode inherent in the model of Katz.

Animals↗

Biological space experiments for the simulation of Martian conditions: UV radiation and Martian soil analogues.

The survivability of resistant terrestrial microbes, bacterial spores of Bacillus subtilis, was investigated in the BIOPAN facility of the European Space Agency onboard of Russian Earth-orbiting FOTON satellites (BIOPAN I -III missions). The spores were exposed to different subsets of the extreme environmental parameters in space (vacuum, extraterrestrial solar UV, shielding by protecting materials like artificial meteorites). The results of the three space experiments confirmed the deleterious effects of extraterrestrial solar UV radiation which, in contrast to the UV radiation reaching the surface of the Earth, also contains the very energy-rich, short wavelength UVB and UVC radiation. Thin layers of clay, rock or meteorite material were shown to be only successful in UV-shielding, if they are in direct contact with the spores. On Mars the UV radiation climate is similar to that of the early Earth before the development of a protective ozone layer in the atmosphere by the appearance of the first aerobic photosynthetic bacteria. The interference of Martian soil components and the intense and nearly unfiltered Martian solar UV radiation with spores of B. subtilis will be tested with a new BIOPAN experiment, MARSTOX. Different types of Mars soil analogues will be used to determine on one hand their potential toxicity alone or in combination with solar UV (phototoxicity) and on the other hand their UV protection capability. Two sets of samples will be placed under different cut-off filters used to simulate the UV radiation climate of Mars and Earth. After exposure in space the survival of and mutation induction in the spores will be analyzed at the DLR, together with parallel samples from the corresponding ground control experiment performed in the laboratory. This experiment will provide new insights into the principal limits of life and its adaptation to environmental extremes on Earth or other planets which and will also have implications for the potential for the evolution and distribution of life.

Bacillus subtilis↗

Radiation analysis for manned missions to the Jupiter system.

An analysis for manned missions targeted to the Jovian system has been performed in the framework of the NASA RASC (Revolutionary Aerospace Systems Concepts) program on Human Exploration beyond Mars. The missions were targeted to the Jupiter satellite Callisto. The mission analysis has been divided into three main phases, namely the interplanetary cruise, the Jupiter orbital insertion, and the surface landing and exploration phases. The interplanetary phase is based on departure from the Earth-Moon L1 point. Interplanetary trajectories based on the use of different propulsion systems have been considered, with resulting overall cruise phase duration varying between two and five years. The Jupiter-approach and the orbital insertion trajectories are considered in detail, with the spacecraft crossing the Jupiter radiation belts and staying around the landing target. In the surface exploration phase the stay on the Callisto surface is considered. The satellite surface composition has been modeled based on the most recent results from the GALILEO spacecraft. In the transport computations the surface backscattering has been duly taken into account. Particle transport has been performed with the HZETRN heavy ion code for hadrons and with an in-house developed transport code for electrons and bremsstrahlung photons. The obtained doses have been compared to dose exposure limits.

Cosmic Radiation↗

Uncertainties in estimates of the risks of late effects from space radiation.

Methods used to project risks in low-Earth orbit are of questionable merit for exploration missions because of the limited radiobiology data and knowledge of galactic cosmic ray (GCR) heavy ions, which causes estimates of the risk of late effects to be highly uncertain. Risk projections involve a product of many biological and physical factors, each of which has a differential range of uncertainty due to lack of data and knowledge. Using the linear-additivity model for radiation risks, we use Monte-Carlo sampling from subjective uncertainty distributions in each factor to obtain an estimate of the overall uncertainty in risk projections. The resulting methodology is applied to several human space exploration mission scenarios including a deep space outpost and Mars missions of duration of 360, 660, and 1000 days. The major results are the quantification of the uncertainties in current risk estimates, the identification of factors that dominate risk projection uncertainties, and the development of a method to quantify candidate approaches to reduce uncertainties or mitigate risks. The large uncertainties in GCR risk projections lead to probability distributions of risk that mask any potential risk reduction using the "optimization" of shielding materials or configurations. In contrast, the design of shielding optimization approaches for solar particle events and trapped protons can be made at this time and promising technologies can be shown to have merit using our approach. The methods used also make it possible to express risk management objectives in terms of quantitative metrics, e.g., the number of days in space without exceeding a given risk level within well-defined confidence limits.

Aluminum↗

DNA fragmentation induced by Fe ions in human cells: shielding influence on spatially correlated damage.

Outside the magnetic field of the Earth, high energy heavy ions constitute a relevant part of the biologically significant dose to astronauts during the very long travels through space. The typical pattern of energy deposition in the matter by heavy ions on the microscopic scale is believed to produce spatially correlated damage in the DNA which is critical for radiobiological effects. We have investigated the influence of a lucite shielding on the initial production of very small DNA fragments in human fibroblasts irradiated with 1 GeV/u iron (Fe) ions. We also used gamma rays as reference radiation. Our results show: (1) a lower effect per incident ion when the shielding is used; (2) an higher DNA Double Strand Breaks (DSB) induction by Fe ions than by gamma rays in the size range 1-23 kbp; (3) a non-random DNA DSB induction by Fe ions.

Cell Line↗

Creation of biological module for self-regulating ecological system by the way of polymerization of composite materials in free space.

The large-size frame of space ship and space station can be created with the use of the technology of the polymerization of fiber-filled composites and a liquid reactionable matrix applied in free space or on the other space body when the space ship or space station will be used during a long period of time. For the polymerization of the station frame the fabric impregnated with a long-life polymer matrix (prepreg) is prepared in terrestrial conditions and, after folding, can be shipped in a compact container to orbit and kept folded on board the station. In due time the prepreg is carried out into free space and unfolded. Then a reaction of matrix polymerization starts. After reaction of polymerization the durable frame is ready for exploitation. After that, the frame can be filled out with air, the apparatus and life support systems. The technology can be used for creation of biological frame as element of self regulating ecological system, and for creation of technological frame which can be used for a production of new materials on Earth orbit in microgravity conditions and on other space bodies (Mars, Moon, asteroids) for unique high price mineral extraction. Based on such technology a future space base on Earth orbit with volume of 10(6) m3 and a crew of 100 astronauts is considered.

Ecological Systems, Closed↗

The features of radiation dose variations onboard ISS and Mir space station: comparative study.

The dynamics of the ISS-measured radiation dose variations since August 2000 is studied. Use is made of the data obtained with the R-16 instrument, which consists of two ionization chambers behind different shielding thicknesses. The doses recorded during solar energetic particle (SEP) events are compared with the data obtained also by R-16 on Mir space station. The SEP events in the solar maximum of the current cycle make a much smaller contribution to the radiation dose compared with the October 1989 event recorded on Mir space station. In the latter event, the proton intensity was peaking during a strong magnetic storm. The storm-time effect of solar proton geomagnetic cutoff decreases on dose variations is estimated. The dose variations on Mir space stations due to formation of a new radiation belt of high-energy protons and electrons during a sudden commencement of March 24, 1991 storm are also studied. It was for the first time throughout the ISS and Mir dose measurement period that the counting rates recorded by both R-16 channels on ISS in 2001-2002 were nearly the same during some time intervals. This effect may arise from the decreases of relativistic electron fluxes in the outer radiation belt.

Electrons↗

Trapped antiprotons produced by cosmic rays in the Earth's magnetosphere.

The existence of significant fluxes of antiparticles in the Earth magnetosphere has been predicted on theoretical considerations in this article. These antiparticles (positrons or antiprotons) at several hundred kilometers of altitudes, we believe are not of direct extraterrestrial origin, but are the natural products of nuclear reactions of the high energy primary cosmic rays (CR) and trapped protons (TP) confined in the terrestrial radiation belt, with the constituents of terrestrial atmosphere. Extraterrestrial positrons and antiprotons born in nuclear reactions of the same CR particles passing through only 5-7 g/cm2 of interstellar matter, exhibit lower fluxes compared to the antiprotons born at hundreds of g/cm2 in the atmosphere, which when confined in the magnetic field of the Earth (in any other planet), get accumulated. We present the results of the computations of the antiproton fluxes at 10 MeV to several GeV energies due to CR particle interactions with the matter in the interstellar space, and also with the residual atmosphere at altitudes of approximately 1000 km over the Earth's surface. The estimates show that the magnetospheric antiproton fluxes are greater by two orders of magnitude compared to the extraterrestrial fluxes measured at energies <1-2 GeV.

Atmosphere↗

Absorbed dose of secondary neutrons from galactic cosmic rays inside the International Space Station.

In this paper, we present the results of Monte-Carlo simulations of the flux and energy spectra of neutrons generated as a result of galactic cosmic ray proton interactions with the material of International Space Station (ISS) inside Zvezda Service Module, the Airlock between Russian and USA segments and one of Russian Research Modules for a full configuration of ISS. Calculations were made for ISS orbit for the energy ranges <10 and >10 MeV for both maximum and minimum of solar activity. To test the accuracy of the calculations the same simulations were made for MIR orbital station and for CORONAS-I satellite and compared with the results of measurements. Calculated and measured fluxes are in reasonable agreement.

Cosmic Radiation↗

Pre-biotic stage of life origin under non-photosynthetic conditions.

Spontaneous assembling of a simplest bacterial cell even if all necessary molecules are present in a solution seems to be extremely rare event and from the scientific standpoint has to be considered as impossible. Therefore, a predecessor of a living cell has to be very simple for providing its self-assembling and at the same time it should be able of progressive increase in complexity. Now phase-separated particles, first of all micelles, are put forward as possible predecessors of living cell. According to the offered working concept only phase-separated particles possessing autocatalytic properties can be considered as predecessors of living cells. The first stage of evolution of these phase-separated autocatalytic systems is the appearance of pre-biotic metabolism providing synthesis of amphiphiles for formation of capsules of these systems. This synthesis is maintained by the energy of a base reaction being a component of a planet-chemical cycle. Catalytic system providing functioning of pre-biotic metabolism is based on multivariate oligomeric autocatalyst, which reproduces itself from monomers, penetrating the particles from the outside. Since the autocatalyst realizes random polymerization then a collection of other oligomers possessing different catalytic functions is produced. In the paper the functioning of multivariate oligomeric autocatalyst in flow reactor is analyzed.

Catalysis↗

"Bioplutonism" and the evolutionary implications of beneficial genes from another biosphere.

Could exogenous genes from another biosphere have aided the evolution of life on Earth's surface over the last half-billion years? That possibility was considered by Thomas Gold in 1992, when he hypothesized that a "deep hot biosphere" (DHB) resides independently well below its cooler surface counterpart. And he suggested that "... in the long term ... there may occasionally be beneficial exchanges of genetic material between microbial life at depth and the surface life." Thus, the question: what evidence is there to support Gold's notion that exogenous genes from the DHB--let us call them "bioplutons"--ever bestowed benefits on the evolution of surface life? In pursuit of this question I drafted a null hypothesis: "Nothing beyond our own biosphere, as we know it today, renders any kind of genetic benefits to biological evolution." After objectively analyzing the evidence and arguments pro and con I failed to reject the null hypothesis, given what we know today, especially the fact that no genetic imprint from the DHB has been identified in eukaryotic genomes. But my conclusion is regarded as tentative, because the fundamentals of Gold's argument, collectively referred to herein as "bioplutonism," might be confirmed eventually with successful probes into the DHB, and with the sampling of its alleged genetic material.

Biological Evolution↗

Identification of 2,3-butanedione (diacetyl) as the compound causing odor events at trace levels in the Llobregat River and Barcelona's treated water (Spain).

A study of organic compounds imparting sweet and buttery odor problems in the Llobregat River (northeast Spain) and in treated water was conducted. Solid-phase microextraction (SPME), gas chromatography-olfactometry, and flavor profile analysis (FPA) were used as analytical methodologies to identify the compound responsible for odor incidents. 2,3-Butanedione (diacetyl) with a concentration range of 0.90-26 microg/l in river water samples entering the water treatment plant was identified as the compound causing the odor events. Flavor profile analysis establishes 0.05 microg/l as its odor threshold concentration (OTC) in water, with an odor recognition concentration of 0.20 microg/l. The analyses were carried out with SPME-GC-MS and parameters affecting SPME extraction such as selection of the fiber (carboxen-polydimethylsiloxane), extraction time (30 min), temperature (60 degrees C), and ionic strength were evaluated. Quality parameters of the optimized method gives good linearity (r2 > 0.999), a limit of detection (0.08 microg/l) similar to the OTC of the compound, and good reproducibility (R.S.D. < 20%). The SPME method was applied to identify the compound causing the odor.

Diacetyl↗

Performances under representative pressure and temperature conditions of the gas chromatography-mass spectrometry space experiment to investigate Titan's atmospheric composition.

In the frame of the calibration of the aerosol collector and pyrolyser, and gas chromatography-mass spectrometry experiments of the Huygens probe arrived at Titan, systematic experimental studies were led to estimate the influence of the operating conditions on the analyses that should have been achieved in the Titan's atmosphere. The primary objective of this study was to estimate the influence of operating conditions variations induced by (i) instrumental modifications made shortly before the probe launch which can have changed the operating pressures; (ii) the change of the probe environmental conditions (pressure, temperature) during its descent in the atmosphere; (iii) a possible deviation of pressure and temperature regulations from their nominal values because of the long journey of the instrument in space, or of other external events. The secondary objective of this work was to create an analytical database that can be used as a reference to treat the chromatograms obtained in situ, and help to identify chromatographically the analyzed species, complementary to mass spectrometry. Beyond the application to a specific instrument, this work was also useful to experimentally estimate the fundamental evolution of the separation as a function of the changes of operating conditions with time. The obtained results show (i) the significant influence of inlet and outlet pressure variation on the time of analysis, but not on the separation power. It thus enables to significantly shorten the analysis duration, and thus to analyze more compounds within the fixed time of analysis of the instrument; (ii) the significant influence of temperature on the retention. In this frame, the enthalpies of exchange between the gas phase and the stationary phase of the species were determined to be used to retrieve the analyzed species in case of deviation of the operating temperature; (iii) that the possible aging of the columns does not have influence on the columns efficiency and separation power; (iv) the analytical capabilities of the gas chromatography-mass spectrometry experiment within operating conditions representative of those encountered in situ. Finally, in spite of possible operating condition changes, it is shown that results coming from the gas chromatograph-mass spectrometer experiment, which are currently under analysis, could bring important information on the Titan's atmosphere and its history.

Atmosphere↗

Analysis of Titan tholin pyrolysis products by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry.

The reddish brown haze that surrounds Titan, Saturn's largest moon, is thought to consist of tholin-like organic aerosols. Tholins are complex materials of largely unknown structure. The very high peak capacity and structured chromatograms obtained from comprehensive two-dimensional GC (GC x GC) are attractive attributes for the characterization of tholin pyrolysis products. In this report, GC x GC with time-of-flight MS detection and a flash pyrolysis inlet is used to characterize tholin pyrolysis products. Identified pyrolysis products include low-molecular-weight nitriles, alkyl substituted pyrroles, linear and branched hydrocarbons, alkyl-substituted benzenes and PAH compounds. The pyrolysis of standards found in tholin pyrolysate showed that little alteration occurred and thus these structures are likely present in the tholin material.

Aerosols↗