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At least 973 records · Page 54Linked to original sources
PubMed2001

A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis.

Dynamin, a high-molecular-weight GTPase, plays a critical role in vesicle formation at the plasma membrane during endocytosis in animal cells. Here we report the identification of a new dynamin homolog in Arabidopsis named Arabidopsis dynamin-like 6 (ADL6). ADL6 is quite similar to dynamin I in its structural organization: a conserved GTPase domain at the N terminus, a pleckstrin homology domain at the center, and a Pro-rich motif at the C terminus. In the cell, a majority of ADL6 is associated with membranes. Immunohistochemistry and in vivo targeting experiments revealed that ADL6 is localized to the Golgi apparatus. Expression of the dominant negative mutant ADL6[K51E] in Arabidopsis protoplasts inhibited trafficking of cargo proteins destined for the lytic vacuole and caused them to accumulate at the trans-Golgi network. In contrast, expression of ADL6[K51E] did not affect trafficking of a cargo protein, H(+)-ATPase:green fluorescent protein, destined for the plasma membrane. These results suggest that ADL6 is involved in vesicle formation for vacuolar trafficking at the trans-Golgi network but not for trafficking to the plasma membrane in plant cells.

Amino Acid Sequence↗
PubMed2005

Golgi-mediated vacuolar sorting of the endoplasmic reticulum chaperone BiP may play an active role in quality control within the secretory pathway.

Quality control in the endoplasmic reticulum (ER) prevents the arrival of incorrectly or incompletely folded proteins at their final destinations and targets permanently misfolded proteins for degradation. Such proteins have a high affinity for the ER chaperone BiP and are finally degraded via retrograde translocation from the ER lumen back to the cytosol. This ER-associated protein degradation (ERAD) is currently thought to constitute the main disposal route, but there is growing evidence for a vacuolar role in quality control. We show that BiP is transported to the vacuole in a wortmannin-sensitive manner in tobacco (Nicotiana tabacum) and that it could play an active role in this second disposal route. ER export of BiP occurs via COPII-dependent transport to the Golgi apparatus, where it competes with other HDEL receptor ligands. When HDEL-mediated retrieval from the Golgi fails, BiP is transported to the lytic vacuole via multivesicular bodies, which represent the plant prevacuolar compartment. We also demonstrate that a subset of BiP-ligand complexes is destined to the vacuole and differs from those likely to be disposed of via the ERAD pathway. Vacuolar disposal could act in addition to ERAD to maximize the efficiency of quality control in the secretory pathway.

Androstadienes↗
PubMed2000

14-3-3 proteins form a guidance complex with chloroplast precursor proteins in plants.

Transit sequences of chloroplast-destined precursor proteins are phosphorylated on a serine or threonine residue. The amino acid motif around the phosphorylation site is related to the phosphopeptide binding motif for 14-3-3 proteins. Plant 14-3-3 proteins interact specifically with wheat germ lysate-synthesized chloroplast precursor proteins and require an intact phosphorylation motif within the transit sequence. Chloroplast precursor proteins do not interact with 14-3-3 when synthesized in the heterologous reticulocyte lysate. In contrast, a precursor protein destined for plant mitochondria was found to be associated with 14-3-3 proteins present in the reticulocyte lysate but not with 14-3-3 from wheat germ lysate. This indicates an unrecognized selectivity of 14-3-3 proteins for precursors from mitochondria and plastids in plants in comparison to fungi and animals. The heterooligomeric complex has an apparent size of 200 kD. In addition to the precursor protein, it contains 14-3-3 (probably as a dimer) and a heat shock protein Hsp70 isoform. Dissociation of the precursor complex requires ATP. Protein import experiments of precursor from the oligomeric complex into intact pea chloroplasts reveal three- to fourfold higher translocation rates compared with the free precursor, which is not complexed. We conclude that the 14-3-3-Hsp70-precursor protein complex is a bona fide intermediate in the in vivo protein import pathway in plants.

14-3-3 Proteins↗
PubMed1991

Targeting of proteins to the outer envelope membrane uses a different pathway than transport into chloroplasts.

The chloroplastic envelope is composed of two membranes, inner and outer, each with a distinct set of polypeptides. Like proteins in other chloroplastic compartments, most envelope proteins are synthesized in the cytosol and post-translationally imported into chloroplasts. Considerable knowledge has been obtained concerning protein import proteins. We isolated a cDNA clone from pea that encodes a 14-kilodalton outer envelope membrane protein. The precursor form of this protein does not possess a cleavable transit peptide and its import into isolated chloroplasts does not require either ATP or a thermolysin-sensitive component on the chloroplastic surface. These findings, together with similar observations made with a spinach chloroplastic outer membrane protein, led us to propose that proteins destined for the outer membrane of the chloroplastic envelope follow an import pathway distinct from that followed by proteins destined for other chloroplastic compartments.

Adenosine Triphosphate↗
PubMed2001

A GIS-based framework for hazardous materials transport risk assessment.

This article presents a methodology for assessment of the hazardous materials transport risk in a multicommodity, multiple origin-destination setting. The proposed risk assessment methodology was integrated with a Geographical Information System (GIS), which made large-scale implementation possible. A GIS-based model of the truck shipments of dangerous goods via the highway network of Quebec and Ontario was developed. Based on the origin and destination of each shipment, the risk associated with the routes that minimize (1) the transport distance, (2) the population exposure, (3) the expected number of people to be evacuated in case of an incident, and (4) the probability of an incident during transportation was evaluated. Using these assessments, a government agency can estimate the impact of alternative policies that could alter the carriers' route choices. A related issue is the spatial distribution of transport risk, because an unfair distribution is likely to cause public concern. Thus, an analysis of transport risk equity in the provinces of Quebec and Ontario is also provided.

Geography↗
PubMed1999

Does social mobility affect the size of the socioeconomic mortality differential?: evidence from the Office for National Statistics Longitudinal Study.

"The effect of social mobility on the socioeconomic differential in mortality is examined with data from the Office for National Statistics Longitudinal Study. The analyses involve 46,980 men aged 45-64 years in 1981. The mortality risk of the socially mobile is compared with the mortality risk of the socially stable after adjustment for their class of origin (their social class in 1971) and class of destination (their social class in 1981) separately. Among those in employment there is some evidence that movement out of their class of origin is in the direction predicted by the idea of health-related social mobility. This evidence, however, seems strongest for causes of death which are least likely to have been preceded by prolonged incapacity. Movement into the class of destination, however, shows the opposite relationship with mortality."

Age Factors↗
PubMed1990

Receptor-ligand complexes are cleared to the open canalicular system of surface-activated platelets.

Human platelets were incubated with gold particles coupled to fibrinogen to label the glycoprotein IIb-IIIa (GPIIb-IIIa) receptor after initial activation of the cells by contact with formvar-coated grid and glass surfaces. Fibrinogen-gold (Fgn-Au) markers were absent on discoid platelets, but diffusely spread over the surface and extended pseudopods of early dendritic cells. Conversion to spread platelets resulted in movement of ligand-receptor complexes away from the cell margin toward cell centres. However, Fgn-Au gold did not concentrate in the central region. Rather, the Fgn-Au, GPIIb-IIIa complexes in the middle of spread platelets appeared to move toward a belt-like, intermediate zone, as did the ligand receptor complexes from the cell margin and pseudopods. The ultimate destination of the mobile receptor-ligand complexes, however, appeared to be channels of the surface-connected open canalicular system (OCS). Fgn-Au was concentrated in OCS channels of most dendritic and a small proportion of spread platelets. The decreased frequency of Fgn-Au filled channels in more transformed platelets may have been due to collapse or evagination of the OCS. Examination of platelets exposed to Fgn-Au after spreading on glass and then prepared for thin sections confirmed that the OCS was the final destination for mobile ligand receptor complexes on surface-activated platelets. Findings of this study are consistent with previous work showing clearance of mobile receptor-ligand complexes to the OCS of platelets activated in suspension.

Blood Platelets↗
PubMed1991

Using a manpower database to model nurse turnaround and return to service.

A manpower database for nurses employed by the Department of Health and Social Services in Northern Ireland has been developed. This database contains information on all nurses employed between March 1977 and July 1988 and consists of data on all posts held in this period, including breaks in service. The authors show how these data may be used to analyse the durations of spells in post and spells out of service prior to departure to a number of destinations for the sister and staff nurse grades. The results are presented in a graphical format which enables us to evaluate the relative proportion of departures to each destination and at what stage in service these departures are likely to occur. Such a methodology provides a valuable tool for the nurse manpower planner, and utilizes personnel data which are routinely collected by most health authorities.

Career Mobility↗
PubMed1993

Clinical electives: setting up an overseas programme in a new medical school.

This paper describes the methods used to establish an overseas elective programme in a new medical school, the objectives and the perceived results. The aims and objectives of the programme are listed. The students who participated in the programme were from the charter class and had completed the second year of a 3-year clinical programme. The methods used to establish the programme are described. Destinations were organized through academic staff contacts. Systematic preparation of students was an important feature of the programme. Comparative costs and methods of financing the programme are described in detail. The results of the programme are analysed together with the form of assessment used and feedback from both the host institution and the student. The importance of monitoring progress while students are abroad is described. The discussion focuses on the benefits derived from an overseas elective programme for both students and teachers. The advantages and disadvantages of different destinations are compared. Further refinements to the programme are discussed including alternative methods of obtaining funding. The importance of developing reciprocal arrangements with other medical schools and institutions throughout the world is emphasized.

Costs and Cost Analysis↗
PubMed2005

A potential novel mechanism for the insertion of a membrane protein revealed by a biochemical analysis of the Plasmodium falciparum cytoadherence molecule PfEMP-1.

Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is exposed on the surface of infected erythrocytes where it both acts as an important pathogenicity factor in malaria and undergoes antigenic variation as a means of immune evasion. Because the mammalian erythrocyte lacks a protein secretory machinery there has been much interest in elucidating the mechanism whereby this protein is transferred from its site of synthesis within the parasite to its final destination. Current opinion favours a mechanism whereby PfEMP-1 becomes cotranslationally inserted into the endoplasmic reticulum of the parasite and is subsequently transported as an integral part of an erythrocyte cytoplasmic membrane system derived from the parasite. Here we show that the solubility characteristics of this protein during several stages of its transport pathway are inconsistent with this view. Instead we propose that the protein is synthesized as a peripheral membrane protein which only when it arrives at its final destination assumes a transmembrane topology. Even in this state, the extractability of the protein with urea suggest that it is anchored in the membrane by protein-protein rather than by protein-lipid interaction.

Amino Acid Sequence↗
PubMed1987

Regulation of the developmental modes in Dictyostelium mucoroides by cAMP and ethylene.

The cellular slime mold Dictyostelium mucoroides-7 (Dm7) and a mutant (MF1) derived from it exhibit clear dimorphism in development depending upon environmental conditions: macrocyst formation occurs during the sexual cycle, and sorocarp formation during the asexual process. As previously reported, exposure of cells to ethylene gas is favorable to macrocyst formation, while exogenously added 3',5'-cyclic adenosine monophosphate (cAMP) induces sorocarp formation. The significance of ethylene and cAMP for the mechanism involved in selection of the developmental pathways was further confirmed by determining the amounts of these substances in macrocyst- or sorocarp-forming cells. Aminooxy-acetic acid (AOA), an inhibitor of ethylene synthesis, was found to switch development of Dm7 and MF1 cells from macrocyst to sorocarp formation by decreasing ethylene production. The cAMP content was shown to be always higher in cells destined for sorocarp formation than in those destined for macrocyst formation, particularly at the aggregation stage. All of the results obtained strongly suggested that the amounts of cAMP and ethylene present, and possibly the ratio between them, may be of great importance for determining which mode of development will be realized.

Aminooxyacetic Acid↗
PubMed1995

Import and insertion of proteins into the mitochondrial outer membrane.

Nuclear-encoded proteins destined for insertion into the mitochondrial outer membrane, follow the same general pathway for import as proteins that are translocated to interior compartments within the organelle. This observation is true both for beta-barrel-type proteins and for proteins that contain hydrophobic alpha-helical transmembrane segments. In this review, we describe what is known about the various steps leading to protein insertion into the outer membrane, and discuss the energetics that favor vectorial translocation into and across this membrane. The selection of the outer membrane during import may involve a lateral release of the translocating polypeptide from the import machinery so that the appropriate domains of the protein become embedded in the lipid bilayer. One type of topogenic domain that can guarantee such selection of the outer membrane is a signal-anchor sequence of the type characterized for the bitopic protein Mas70p. It is suggested that a signal-anchor sequence selective for the mitochondrial outer membrane causes abrogation of polypeptide translocation and triggers the release of the transmembrane segment into the surrounding lipid bilayer, prior to any possibility for the commitment of translocation to the interior of the organelle. Specific structural features of the signal-anchor sequence specify its orientation in the membrane, and can confer on this sequence the ability to form homo-oligomers and hetero-oligomers. Strategies other than a signal-anchor sequence may be employed by other classes of proteins for selection of the outer-membrane. Of note is the ability of the outer-membrane import machinery to catalyze integration of the correct set of proteins into the outer-membrane bilayer, while allowing proteins that are destined for integration into the bilayer of the inner membrane to pass through unimpeded. Again, however, different proteins may employ different strategies. One model proposes that this can be accomplished by a combination of a matrix-targeting signal and a distal stop-transfer sequence. In this model, the formation of contact sites, which is triggered when the matrix-targeting signal engages the import machinery of the inner membrane, may prevent the outer-membrane translocon from recognizing and responding to the downstream stop-transfer domain. This allows the transmembrane segment to pass across the outer-membrane, and subsequently integrate into the inner membrane.

Animals↗
PubMed1996

Barriers to the use of urban medical services by rural and remote area households.

People from rural and remote areas commonly need to attend provincial and metropolitan cities for specialist care. There decisions to make such trips 'away' involve a number of non-medical considerations that include economic, emotional and social factors. This paper reports the results of two surveys that sought information about the types and importance of non-medical considerations taken into account by rural and remote Queensland householders when faced with a trip away. In addition, the problems encountered by respondents on their last trip away are reported and discussed. The data revealed that important considerations taken into account when planning the trip were predominantly related to urgency, household organisation and the costs likely to be incurred where away. A number of avoidable problems occurring at destination were also described. Generally, remote area respondents saw these impediments as more serious barriers to seeking care than did rural area respondents. When respondents were further asked to identify major problems associated with their last trip to an urban facility, problems at the destination figured more prominently, particularly problems directly related to the lack of understanding of the transport and distance needs of rural people. With one exception, these problems were reported by similar proportions of rural and remote area respondents. These are matters that merit high priority attention in any programs to enhance access to specialist medical services by people in rural and remote areas.

Emergencies↗
PubMed1987

Fiber type differentiation and myosin expression in regenerating rat muscles.

The relation between fiber type differentiation and the expression of slow and fast myosin isoforms was examined in regenerating rat muscles after injection of a myotoxic agent, bupivacaine. The histochemical myosin ATPase reaction for fiber typing demonstrated that immature type 2C fibers differentiated into type 1, 2A and 2B fibers. Slow and fast myosin isoforms were demonstrated immunohistochemically using antibodies raised against myosins extracted from the slow-twitch soleus and fast-twitch tensor fasciae latae muscles of mature guinea pigs (anti-SOL, anti-TFL). The results showed that immature type 2C fibers destined to differentiate into type 1 fibers first reacted with anti-TFL only, and later reacted with both anti-TFL and anti-SOL, whereas those destined to differentiate into type 2A and 2B fibers reacted with anti-TFL only throughout regeneration. The significance of the myosin isoforms that react with anti-TFL in immature type 2C fibers was discussed.

Adenosine Triphosphatases↗
PubMed2004

Patterns of active transport in 11-12 year old Australian children.

OBJECTIVES: To describe the habitual transport patterns of 11 to 12-year-old children in Australia, to determine the personal and environmental factors associated with active transport (AT), and to quantify how much AT contributes to overall daily energy expenditure (EE). METHODS: The participants in this study were 136 children aged 11-12 year olds from eight randomly chosen primary schools in Adelaide, South Australia. Each child recalled their trips on two school days and a non-school day. Mass and stature were measured, and children completed a computerised activity recall and a neighbourhood satisfaction questionnaire. Trips were categorised according to their destination, child and parent dissatisfaction with the neighbourhood, and the gender, socio-economic status (SES), BMI and activity levels of the children undertaking them. These categories, along with the distance to the destination, were used as independent variables in a logistic regression model, with trip mode (passive versus active) as the dependent variable. RESULTS: Children made an average of 1.0 active trips per day, with a median trip length of 0.63 km, while the median total distance covered actively per child per day was 0.61 km. Twenty-six per cent of children did no AT over the three days, and 67% did no AT on a weekend day. Distance was by far the strongest predictor of the likelihood that a trip would be active. Trips made by girls were less likely to be active compared with boys. Trips to the shops were less likely to be active than trips to school. Children's AT accounted for 1.3% of their daily EE. CONCLUSIONS AND IMPLICATIONS: The active transport levels of children were very low. Interventions should focus on making neighbourhoods safer and more accessible to children and should promote bicycle use.

Child↗
PubMed1982

Moving back vs. moving on: the concept of home in the decision to remigrate.

"This study examines the effect of information and psychic costs on the remigration propensity of the U.S. labor force. Specifically, the study investigates how the proximity of a potential migration destination to a previous residence, and familiarity with this residence, affect information and psychic costs, and thus, remigration propensity. In this respect it is hypothesized that familiarity with, and location of, a prior residence are significant determinants of both the migration destination and the allocative efficiency of the remigration process." Several specific hypotheses are developed and tested using data on interstate, nonreturn, repeat migration of the white male labor force over the period 1965-1970.

Americas↗
PubMed2006

Religion, social mobility and education in Scotland.

The relationship among religion, education and social mobility in Scotland is analysed statistically using the Scottish Household Survey of 2001. The large sample size allows much greater statistical power for this purpose than any previous source, and thus allows a more reliable assessment of claims that the stratifying effect of religion in Scotland may have declined. The questions investigated are as follows. What are the religious differences in the distributions of class origins and class destinations, in the movement between these (absolute mobility), and in the association of these (relative mobility, or social fluidity)? Do changes in social fluidity across cohorts vary among people with different religious affiliation? Are there religious differences in the association of origins and education, in the association of education and destinations, or in the role of education in social fluidity, and do any of these vary over cohorts? The conclusions are that, in younger cohorts, there is no religious difference in social status, and that in older cohorts Catholics are generally of lower status than Protestants and the non-religious. Social fluidity does not, however, vary among religious groups, even for older cohorts, and does not change over time. The reason for convergence in social status of religious groups over time is probably the equalizing of educational attainment among the groups: there is no evidence for any of the cohorts that the labour-market rewards to education differ by religion.

Education↗
PubMed1999

Differential plasma membrane targeting of voltage-dependent calcium channel subunits expressed in a polarized epithelial cell line.

1. Voltage-dependent calcium channels (VDCCs) show a highly non-uniform distribution in many cell types, including neurons and other polarized secretory cells. We have examined whether this can be mimicked in a polarized epithelial cell line (Madin-Darby canine kidney), which has been used extensively to study the targeting of proteins. 2. We expressed the VDCC alpha1A, alpha1B or alpha1C subunits either alone or in combination with accessory subunits alpha2-delta and the different beta subunits, and examined their localization immunocytochemically. An alpha1 subunit was only targeted to the plasma membrane if co-expressed with the accessory subunits. 3. The combination alpha1C/alpha2-delta and all beta subunits was always localized predominantly to the basolateral membrane. It has been suggested that this is equivalent to somatodendritic targeting in neurons. 4. In contrast, the alpha1B subunit was expressed at the apical membrane with all the accessory subunit combinations, by 24 h after microinjection. This membrane destination shows some parallels with axonal targeting in neurons. 5. The alpha1A subunit was consistently observed at the apical membrane in the combinations alpha1A/alpha2-delta/beta1b or beta4. In contrast, when co-expressed with alpha2-delta/beta2a, alpha1A was clearly targeted to the basolateral membrane. 6. In conclusion, the VDCC alpha1 subunit appears to be the primary determinant for targeting the VDCC complex, but the beta subunit can modify this destination, particularly for alpha1A.

Animals↗