Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “egg quality”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 955 records · Page 53Linked to original sources

Dietary calcium levels in pre-lay and lay diets in Leghorn pullets.

Four experiments over a period of four years with 1350 first-year pullets were designed to determine the influence of the calcium and phosphorus levels in the pre-lay diets on the growing birds and on their subsequent performance. Several dietary calcium levels in the lay period were studied, as well as the interaction between the pre-lay and lay diets. During the growing phase the pullets fed 3.0% calcium and 0.4% phosphorus had significantly (P less than 0.05) lower body weight at 20 weeks of age than thep ulletsin the other pre-lay treatments. Feed consumption and feed efficiency were also adversely affected by this treatment, and sexual maturity was significantly (P less than 0.05) delayed. Early lay mortality from this treatment was evident. On the basis of egg production, egg weight, shell deformation and percent poorly shelled eggs (PSE) at point-of-lay, the pre-lay diets did not influence lay performance nor was there an interaction between any combination of pullet and lay diets. As the calcium levels of the lay diets increased, egg production, feed consumption and shell rigidity increased. The 1.5% calcium level did not maintain bone mineralization as compared to the 3.0 and 4.5% calcium levels. It was noted that shell quality on the basis of PSE and deformation does not begin to deteriorate immediately after the onset of production. In fact there is a short period of time where the egg shells improve and then there is a gradual decline in shell rigidity. The layers fed the lay diet containing 1.5% calcium produced eggs weighing an average of 57.4 g. This was significantly (P less than 0.05) lower than the eggs of the 2.3, 3.0 or 4.5% calcium fed groups which produced eggs weighing 59.1, 58.6 and 59.4 g. respectively.

Animal Feed↗

The impact of hormonal fluctuations on female vocal folds.

PURPOSE OF REVIEW: Sex hormone fluctuations were shown to affect female vocal folds and laryngeal function. Laryngeal changes are evident throughout the span of life, starting at puberty with the arousal of the hormonal system, fluctuating systematically during the reproductive years with the menstrual cycle, and then changing again with the decline of hormonal activity at menopause. This paper reviews recent developments in this field. RECENT FINDINGS: Early studies that explored this relation were based merely on subjective impressions of voice quality, recent studies have used more objective tools for examining this relation, including histologic observations, stroboscope, electroglottography (EGG), and computerized acoustic analyses. In these studies, the larynx was shown to be a hormonal target organ and, as such, sex hormones affect its morphology, histology, and function, similar to their effect on the genitals and other organs. SUMMARY: Examining the relation between sex hormones and the larynx could assist in understanding the mechanisms of voice production, and it could provide the clinician with supplemental diagnostic information on different medical conditions.

Adolescent↗

Transcriptomic analysis of eggs, rediae and cercariae reveal stage-specific adaptations in the rumen fluke Calicophoron daubneyi.

Rumen flukes, particularly the trematode Calicophoron daubneyi, are emerging parasites of livestock in Europe, yet transcriptomic insights into their environmental and intermediate host stages remain limited. Here, we present a comprehensive transcriptomic analysis of eggs at three distinct developmental stages (freshly excreted, early developmental and eye-spot stages), as well as rediae and cercariae, of C. daubneyi. High-quality RNA-sequencing (RNA-seq) datasets revealed both shared and stage-specific transcriptional profiles with each developmental stage exhibiting its own distinct expression pattern. Subsequent GO-Term enrichment analyses revealed that fully embryonated eggs in eye-spot-stage especially upregulated genes related to cilia assembly, movement and motility, reflecting preparation for miracidial hatching and host-seeking behavior. Rediae showed enhanced transcription of genes involved in diverse metabolic and biosynthetic processes, supporting rapid asexual proliferation within the snail intermediate host. Cercariae exhibited predominant upregulation of genes associated with signal transduction and energy metabolism, indicating the adaptation to its changing environmental conditions. These findings provide the first transcriptomic insights into the biology of C. daubneyi outside the definitive host, reveal molecular mechanisms underlying development, transmission and adaptation to a changing environment and identify stage-specific genes as potential targets for interventions aimed at disrupting the parasites life cycle and controlling rumen flukes in the future.

Animals↗

Reproductive performance of medium white breeder toms fed diets with varying metabolizable energy levels and 7% crude protein.

An experiment was conducted to study the effects of feeding diets composed of triticale (var: Flora) with varying ME levels and 7% CP on fertilizing capacity and semen quality of Medium White breeder toms. Thirty-nine breeder toms were fed ad libitum from 30 to 54 wk of age one of four dietary treatments: 14% CP with 2,892 kcal ME/kg; 7% CP with 2,919 kcal ME/kg; 7% CP with 2,719 kcal ME/kg; and 7% CP with 2,539 kcal ME/kg. Semen volume (SV), packed spermatozoa volume (PSCV), BW, testicular weight (TW), and fertilizing capacity of semen were measured. No differences for SV, PSCV, TW, calculated daily feed, and estimated energy consumption were observed among the dietary energy levels fed. The estimated daily protein intake was higher (P less than .05) for toms fed the 14% 2,892-ME diet than 2,539, 2,719, and 2,919-ME diets containing 7% CP. The 7% 2,919-ME diet reduced (P less than .05) the fertilizing capacity of spermatozoa, whereas the 7% 2,539-ME diet decreased the percentage of fertile eggs hatched. Under the conditions of this experiment, Medium White breeder toms maintained fertilizing capacity and semen quality when provided a diet containing 7% CP with 2,719 kcal ME/kg.

Animals↗

Effect of initial product temperature and initial pH on foaming time during vacuum evaporation of liquid whole eggs.

During earlier studies on vacuum concentration of liquid egg white, the phenomena of foaming during the initial stages of the process were reported. In these studies, it was also shown that no concentration took place during the foaming period that varied from test to test. To minimize the total process time, this present study was undertaken to investigate what variables contributed to foaming, how they could be controlled, and what effect they had on product quality and functional properties. This study investigated the relationships among initial product temperature, initial pH, and foaming of liquid whole eggs. Two temperatures (9 and 20 C) and three pH levels (6.5, 7.3, and 8.5) were studied using a vacuum evaporation system with a maximum vacuum of 5 kPa. Tests showed that higher initial pH levels had decreased foaming times. At the end of foaming experiments, the liquid whole egg was evaluated to determine the extent of functional property change during foaming. A decrease in foaming time resulted in a decrease in whip time. The cakes made from the processed liquid whole egg had larger volumes than those from the unprocessed control. Furthermore, the liquid whole egg, which foamed the longest, had higher (P < 0.05) cake volumes. Our current experiments also verified our earlier findings that no product concentration takes place during the foaming process.

Animals↗

Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting.

The goal of the study was to investigate the effect of antioxidant supplementation on the quality of frozen-thawed flow cytometrically sorted bull spermatozoa. Twelve ejaculates from two Holstein Friesian bulls were sorted according to the Beltsville Sperm Sexing Technology. Each ejaculate was divided into three parts and processed as (i) unsorted controls, (ii) according to a standard sorting protocol and (iii) in the presence of different antioxidants (S-AO). Cooling and freezing of the samples were performed in the same way for all three groups, except that antioxidants were added to the TRIS-egg-yolk freezing extender for those semen samples that were already sorted in the presence of antioxidants. The semen quality in frozen-thawed samples was determined by morphology analysis immediately after thawing, motility estimation in a thermo-resistance test after 0, 6, 12 and 24 h incubation at 37 degrees C and Fluorescein isothiocyanate conjugated PNA/propidium iodide (FITC-PNA/PI) staining after 0, 12 and 24 h of incubation at 37 degrees C. There was a significantly higher (p < 0.05) percentage of motile spermatozoa in S-AO samples in comparison to unsorted frozen-thawed control at 0, 6 and 24 h after thawing and compared with normally sorted samples at all times after thawing. The percentage of damaged acrosomes was significantly lower (p < 0.05) in S-AO samples than in the unsorted controls (20.8 +/- 6.9% vs 30.3 +/- 12.0%). The percentage of morphologically abnormal spermatozoa in this group was significantly lower (p < 0.05) than in the unsorted controls and normally sorted samples (25.8 +/- 5.2%, 36.0 +/- 12.5% and 35.1 +/- 7.4%, respectively). Analysis of frozen-thawed spermatozoa with FITC/PI revealed no significant difference in membrane integrity at 0 and 12 h after sorting, but after 24 h of incubation the S-AO samples had a significantly higher (p < 0.001) percentage of spermatozoa with intact membranes in comparison to unsorted controls and normally sorted semen (40.7 +/- 6.3%, 7.8 +/- 4.7% and 7.4 +/- 4.6%, respectively). The percentage of acrosome-reacted spermatozoa was significantly lower (p < 0.05) in the S-AO samples than in the unsorted controls (14.1 +/- 7.5%, 23.4 +/- 5.4% and 28.8 +/- 6.3% vs 25.9 +/- 14.4%, 38.5 +/- 16.7% and 79.8 +/- 4.1%, for 0, 12 and 24 h after thawing, respectively) and in comparison to normally sorted semen 24 h after thawing (67.3 +/- 10.0%). This study demonstrates the highly protective effects of antioxidants on the quality of flow cytometrically sorted frozen-thawed bull spermatozoa.

Acrosome↗

Common practices of voice therapists in the evaluation of patients.

This study surveys voice therapists regarding common diagnostic practices in patients referred for therapy with the diagnosis of muscle tension dysphonia (broadly defined as the "hyperfunctional" component of the dysphonia). Through postings on the e-mail list of the ASHA special interest division on voice, speech pathologists with at least 3 years' experience in stroboscopy and acoustic instrumentation were invited to complete the survey. Results from 53 completed surveys demonstrated that voice quality and patient self-perception are the sole assessments performed by all therapists. Voice quality, observation of body posture and movement, and probing the patient's ability to alter voice production are each significantly more likely to be performed than the more objective stroboscopic, acoustic, aerodynamic, and EGG assessments. Further, the tasks of defining specific therapy session goals and helping the patient to achieve a particular target skill are considered best served by measures of vocal quality, observation of body position and movement, and judging the patient's ability to alter voice production. For definition of the overall therapy goal, stroboscopy and patient perception scales are added to all of the subjective assessment measures as being important. Acoustic data are considered most important for patient reinforcement and outcomes assessment. Implications of these findings are discussed, and topics for further exploration are identified.

Data Collection↗

Statistical analysis of factors affecting fertilization rates and clinical outcome associated with intracytoplasmic sperm injection.

OBJECTIVE: To identify and evaluate the statistically significant predictors of intracytoplasmic sperm injection (ICSI) fertilization rates and clinical pregnancy in a single population using appropriate statistical techniques. DESIGN: Retrospective study. SETTING: Fertility and Endocrinology Center, University of Washington Medical Center, Seattle, Washington. PATIENT(S): Four hundred forty-one patients undergoing their first attempt at IVF-ICSI from January 1, 1999, to May 21, 2001. INTERVENTION(S): Each ICSI procedure for an individual patient was performed by a single operator. Sperm parameters, oocyte age, culture condition, ICSI technique, and ICSI operator were assessed as variables influencing the fertilization rate. We also assessed the impact of patient age, serum E(2) concentration on the day of hCG administration, embryo quality, and number of embryos transferred on the probability of achieving a clinical pregnancy. MAIN OUTCOME MEASURE(S): Fertilization rate and clinical pregnancy. RESULT(S): The 2 pronuclei (2PN) rate was significantly correlated with sperm motility, and there were significant differences in the 2PN rates among the ICSI operators. There was no difference in the 2PN rate among different sperm types or among the eight laboratory incubators or whether the eggs were cultured individually or in groups. Patient age, serum E(2) concentration on the day of hCG administration, embryo quality, and number of embryos transferred were all statistically significant predictors of clinical pregnancy. CONCLUSION(S): In our program, sperm motility and ICSI operator are the two most important predictors for the ICSI fertilization rate in vitro. Patient age, serum E(2) concentration on the day of hCG administration, embryo quality, and number of embryos transferred were all statistically significant predictors of clinical pregnancy.

Adult↗

Role of N-acetylglutamate concentration and ornithine transport into mitochondria in urea synthesis of rats given proteins of different quality.

The purpose of this study was to find whether the concentration of N-acetylglutamate and ornithine transport into mitochondria would regulate urea synthesis when the dietary protein quality was manipulated. Experiments were done on three groups of rats given diets containing 10 g of gluten, 10 g of casein, or 10 g of whole egg protein/100 g for 10 days. The plasma concentration and urinary excretion of urea, the liver concentration and synthesis of N-acetylglutamate, the liver concentrations of glutamate and lysine, and the liver ornithine transport into mitochondria increased with the decrease in quality of dietary protein. A reverse correlation was observed between the activities of urea cycle enzymes, the plasma concentration of arginine, and urinary excretion of urea under these conditions. N-Acetylglutamate concentration and ornithine transport into mitochondria in the liver were closely correlated with the excretion of urea. These results suggest that greater N-acetylglutamate concentration and ornithine transport into isolated mitochondria in the liver of rats, given the lower quality of protein, stimulate urea synthesis and that the concentrations of glutamate and lysine in the liver are at least partly related to the hepatic N-acetylglutamate synthesis and ornithine transport, respectively.

Animals↗

The effect of cooling rate before freezing and the temperature of the semen upon addition of DMSO on the fertilizing capacity of chicken semen stored at - 196 degrees C.

Semen was collected from Single Comb White Leghorn roosters, diluted 1:4 with Beltsville Poultry Semen Extender at 35 degrees C and cooled at various rates to 5 degrees C. DMSO was added to the semen between 10 and 120 min after ejaculation at temperatures between 15 and 5 degrees C, respectively. Following the addition of DMSO, the semen was allowed to equilibrate for 2 h in a 5 degrees C environment. The semen was then frozen at 1 degree C per min from 5 degrees C to - 20 degrees C, transferred into liquid nitrogen vapour for 4 to 10 min and then immersed in liquid nitrogen for 4 to 60 days. The thawed semen was inseminated on two consecutive days and fertility was calculated during 5 and 7 days commencing on the second day after the last insemination. In general, fertility was unaffected by the rate of cooling and the temperature at which the DMSO was added. In one trial, however, the fertilizing capacity was significantly greater if the DMSO was added within 45 min when the temperature of the semen was 15 degrees C. Approximately 42 to 71 p. 100 of eggs laid by hens during the 5 day period after the second insemination were fertile regardless of the method of cooling or the temperature of the semen when the DMSO was added. The motility of the ejaculates after thawing and before insemination varied between 15 and 55 p. 100 and this measure of physiological quality was a poor indicator of fertilizing capacity. Embryonic mortality was unaffected by the cooling and freezing procedure and hatchability of fertile eggs was not significantly different for the hens inseminated with either fresh or frozen semen.

Animals↗

Relationship between fluctuating asymmetry and fitness within and between stressed and unstressed populations of the wolf spider Pirata piraticus.

Although developmental instability, measured as fluctuating asymmetry (FA), is expected to be positively related to stress and negatively to fitness, empirical evidence is often lacking or contradictory when patterns are compared at the population level. We demonstrate that two important properties of stressed populations may mask such relationships: (i) a stronger relationship between FA and fitness, resulting in stronger selection against low quality (i.e. developmental unstable) individuals and (ii) the evolution of adaptive responses to environmental stress. In an earlier study, we found female wolf spiders Pirata piraticus from metal exposed populations to be characterized by both reduced clutch masses and increased egg sizes, the latter indicating an adaptive response to stress. By studying the relationship between these two fitness related traits and levels of FA at individual level, we here show a significant negative correlation between FA and clutch mass in metal stressed populations but not in unstressed reference populations. As a result, levels of population FA may be biased downward under stressful conditions because of the selective removal of developmentally unstable (low quality) individuals. We further show that females that produced larger eggs in stressed populations exhibited lower individual FA levels. Such interaction between individual FA and fitness with stress may confound the effect of metal stress on FA, resulting in an absence of relationships between FA, fitness and stress at the population level.

Adaptation, Physiological↗

[Evaluation of the effect of enriching rice protein with lysine and egg protein].

The experimental biological value (BVe) of rice enriched with lysine and egg protein was studied in growing Wistar male rats with the use of new parameters for the evaluation of the new food protein quality: potential biological value (BVp) and compensation coefficient (C). Pure protein utilization and body weight of rats receiving the lysine-enriched diet were significantly higher than those in the animals given the rice ration. Similar values were recorded in the animals kept on the diet containing rice with egg protein. The new parameters (BVp and C) enable the evaluation of the food protein aminogram and estimation of the effect of rice enrichment with lysine and egg protein.

Animals↗

Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity.

The effects of mannan oligosaccharide (MOS) supplementation in broiler breeder diets on egg production, hatchability, fertility, and immunity were examined by randomly allocating 60-wk-old breeders to eight replicates (four replicates of two diet treatments) in a cage trial, and 42-wk-old broiler breeders to 12 replicates (six replicates of two dietary treatments) in a deep litter trial. Breeders were fed corn-soybean meal based diets with (MOS group) or without MOS or antibiotics (control group). Egg production, hatchability, and related parameters were measured for 8 and 12 wk in the cage trial and deep litter trial, respectively. In the cage trial, semen quality traits and antibody responses were recorded after 6 wk of supplementation. Antibody responses in the parents and progeny were measured after 4 wk of supplementation in the deep litter trial. Dietary MOS had no consistent influence on egg production in either trial. In the cage trial, higher (P < or = 0.05) hatchability, concomitant with consistently lower infertile and dead-in-shells (DIS), was evident in the MOS group. Furthermore, sperm density increased in the MOS group. Conversely, no difference was observed in terms of proportion of live sperm. In the deep litter trial hatchability on total eggs set and DIS were not affected by MOS inclusion in the diet, but hatchability on fertile eggs set and fertility was higher (P < or = 0.05) in the MOS group during the second, third, and fourth periods. Antibody responses against infectious bursal disease virus (IBDV) were higher (P < or = 0.05) in the MOS group in both trials. Maternal antibody titers in progeny were also influenced (P < or = 0.05) by MOS supplementation. These data show that supplemental MOS improved sperm density, antibody titers, and all the production traits excluding egg production in the broiler breeders.

Animals↗

Wild intersex roach (Rutilus rutilus) have reduced fertility.

Endocrine-disrupting chemicals, known to be present in the environment, have great potential for interfering with reproductive health in wildlife and humans. There is, however, little direct evidence that endocrine disruption has adversely affected fertility in any organism. In freshwater and estuarine fish species, for example, although a widespread incidence of intersex has been reported, it is not yet known if intersexuality influences reproductive success. The purpose of this study was, therefore, to determine gamete quality in wild intersex roach (Rutilus rutilus) by assessing sperm characteristics, fertilization success, and ability to produce viable offspring. The results clearly demonstrate that gamete production is reduced in intersex roach. A significantly lower proportion of moderately or severely feminized fish (17.4% and 33.3%, respectively) were able to release milt compared with normal male fish from contaminated rivers (in which 97.6% of the males were able to release milt), reference male fish (97.7%), or less severely feminized intersex fish (experiment 1: 85.8%, experiment 2: 97%). Intersex fish that did produce milt produced up to 50% less (in terms of volume per gram of testis weight) than did histologically normal male fish. Moreover, sperm motility (percentage of motile sperm and curvilinear velocity) and the ability of sperm to successfully fertilize eggs and produce viable offspring were all reduced in intersex fish compared with normal male fish. Male gamete quality (assessed using sperm motility, sperm density, and fertilization success) was negatively correlated with the degree of feminization in intersex fish (r = -0.603; P < 0.001) and was markedly reduced in severely feminized intersex fish by as much as 50% in terms of motility and 75% in terms of fertilization success when compared with either less severely feminized intersex fish or unaffected male fish. This is the first evidence documenting a relationship between the morphological effects (e.g., intersex) of endocrine disruption and the reproductive capabilities of any wild vertebrate. The results suggest that mixtures of endocrine-disrupting substances discharged into the aquatic environment could pose a threat to male reproductive health.

Animals↗

Quality of transgenic laboratory strains of Cochliomyia hominivorax (Diptera: Calliphoridae).

Genetically modified, mass reared insects present novel possibilities for the future of insect control. One concern about manipulation of insects is a possible loss of strain quality due to the introduction of a foreign gene of any sort into the insect genome. Eight transgenic strains of screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), were compared with the wild-type parental laboratory strain in laboratory culture. Measurements of average fertility, fecundity, larval productivity, and longevity were analyzed. Two transgenic strains had significantly lower larval productivity than controls, one of which was explained by a homozygous lethal insertion of the transgene. Another strain produced significantly fewer eggs than controls. Overall strain characteristics, including measurements from egg, larva, pupa, and adult stages, were compared. Transgenic colonies did not consistently show significantly lower individual or aggregate strain quality characteristics than the control parental colony; hence, the presence of the transgene used to produce the strains tested did not incur a discrete cost to the colonies of laboratory-reared C. hominivorax.

Animals↗

Stress reduces the quality of gametes produced by rainbow trout.

In this study we have used the rainbow trout as a model animal to study the biological consequences of stress in terms of gamete quality and quantity. Groups of 30 mature male and female rainbow trout were subjected to repeated acute stress during the 9 mo prior to spawning. Time of ovulation, fecundity, and egg size were recorded in mature females, and sperm counts were carried out on the milt from the male fish, from both the stressed and control groups. Eggs from ovulated females were fertilized with milt from males subjected to the same treatment regime. Approximately 300 eggs from each female were fertilized with a sperm dilution of 10(-3) in diluent. Subsequent development of the fertilized eggs was then monitored. There were no differences in somatic weight or length between the two groups at the end of the experiment, but exposure of rainbow trout to repeated acute stress during reproductive development resulted in a significant delay in ovulation and reduced egg size in females, significantly lower sperm counts in males, and, perhaps most importantly, significantly lower survival rates for progeny from stressed fish compared to progeny from unstressed control fish. Hence, stress reduces the quality of gametes produced by rainbow trout.

Animals↗

The supramolecular organization of ovomucin. Biophysical and morphological studies.

Ovomucin participates in the ovomucin-gel-forming properties because of its shape and its ability to interact in a specific spatial organization. Purified from chicken egg-white by exclusion chromatography with Sephacryl S-300 and Sepharose CL-2B and analysed by light-scattering, it exhibited an Mr of about 40 x 10(6). This large Mr can be explained by the aggregation of polymers that can be degraded into 3 x 10(6)-Mr fragments by reduction with dithiothreitol. The values for hydrodynamic parameters such as Mr, radius of gyration, hydrodynamic radius, mass per unit length and combinations of them suggested that ovomucin is a linear and highly flexible molecule conferring upon it a random-coil-like structure in 0.2 M-NaCl solution. Analysis of the ovomucin molecules by electron microscopy revealed its linear character but also indicated a lower Mr than that obtained in the light-scattering experiments. By temperature-induced non-specific aggregation of an ovomucin solution containing other globular egg proteins, an attempt was made to find out what conditions are required for gel formation and to examine the quality of aggregation that is obtained under these conditions. Results show that the viscosity of the solution did not increase after heat treatment. Apparently, in the ovomucin gel, specific spatial organization of the ovomucin molecules is required for hydrogel formation.

Animals↗