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PubMed2004

[Dynamics of indole alkaloids in wheat varieties with various resistance to wheat aphid Sitobion avenae].

UV spectrometry measurement on the indole alkaloids (IA) contents of seven wheat varieties (KOK1679, My295, Li, Han4564 and Yanda1817, resistant to S. avenae; Shaanximazha and Lovrin10, susceptible to S. avenae) showed that during tillering and stem elongation stages, KOK1679, My295 and Li had a higher IA content (> 0.5 mg.g-1 FW); and during heading and anthesis stages, the penultimate leaves of all five resistant varieties contained higher IA (> 0.5 mg.g-1 FW). The IA content was lower in the flat leaves of both resistant and susceptible varieties, but was significantly higher in the ears of resistant varieties (up to 0.645 mg.g-1 FW in KOK1679) than in those of susceptible ones. It was showed that the IA content in wheat's ear played an important role in the resistance to S. avenae.

Animals↗
PubMed1978

[A new culture medium for growth and sporulation of Entomophthorales species, pathogens of aphids (author's transl)].

No one of the 3 specific egg yolk fractions (neutral lipids, polar lipids and proteins) was necessary for growth and sporulation of species such as Entomophthora aphidis, E. phalloides and E. thaxteriana, normally grown on egg yolk media. Egg yolk could be replaced by a sunflower oil-yeast extract mixture. E. thaxteriana was especially well studied. Various animal and vegetable fats and even chemically pure triglycerides could replace sunflower oil as a carbon source. Yeast extract was the best nitrogen source tested. In liquid culture, sporulation was directly dependent on yeast extract concentration. When yeast extract concentration was held constant, an increase in oil concentration did not produce an increase in sporulation. High dextrose concentrations inhibited resting spore formation. Liquid cultures of E. thaxteriana regularly produced 3 x 10(6) azygospores/ml in a 20 l fermentor.

Animals↗