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PubMed1976

Identification of Propionibacterium acnes and related organisms by precipitin tests with trichloroacetic acid extracts.

The serological identification of Propionibacterium acnes, P. granulosum, and P. avidum, using trichloroacetic acid extracts, is described. With antisera prepared against reference strains, the method has been tested on 142 strains recently isolated from human skin. All except two of the strains could be identified serologically, and there was excellent agreement between the serological results and the fermentation pattern of the strains. Two serological types of P. acnes and two of P. avidum were identified, but only one of P. granulosum.

Antigens, Bacterial↗
PubMed1976

A precipitin-inhibition test on denatured bloodstains for the determination of human origin.

A new method has been developed for determination of the species of origin of denatured bloodstains. Antiserum with known titer is incubated with the denatured bloodstain. If the bloodstain has the same origin as the antiserum, a substantial amount of antibody will be absorbed by the stain, resulting in an attenuation of the antiserum titer. By reacting the supernatant with a known serum by using immunodiffusion it is possible to detect any appreciable attenuation of the antiserum titer. We have been able to determine the species of origin of various denatured bloodstains using this inhibition method.

Blood Stains↗