Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Insect Viruses”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 955 records · Page 53Linked to original sources
PubMed1972

Laboratory studies on the transmission of western equine encephalitis virus by Saskatchewan mosquitoes. I. Culex tarsalis.

A Saskatchewan strain of the mosquito Culex tarsalis, transmitted a local strain of western equine encephalitis virus from chick to chick, between four and 44 days after an infective blood meal. At incubation temperatures of 69 and 75 degrees F, 120 transmissions occurred out of a possible 141, and all but seven of these were by single infected mosquitoes. At 75 degrees F virus titers in individual mosquitoes were more uniform and transmission was more efficient, than at 69 degrees F, although infection rates were similar at both temperatures. The minimum concentration of virus required to infect 50% of C.tarsalis was 10(2.5) intracerebral three-week old mouse LD(50) per 0.03 ml of donor blood. These findings provide direct evidence that C. tarsalis of Saskatchewan is a highly efficient vector of western equine encephalitis virus.

Animals↗
PubMed2005

Characterization of dengue-2 virus binding to surfaces of mammalian and insect cells.

The binding of dengue type 2 (DEN-2) virus to mammalian (LLC-MK(2) and Vero) and mosquito (C6/36 and AP61) cell surfaces was investigated by a virus-binding assay using purified (3)H-labeled DEN-2 virus. The DEN-2 virus binding to all four cell types was specific and saturable, indicating the presence of a single class of receptors (ranging from 3.7 x 10(3) to 3.5 x 10(4) receptors/cell) with a high affinity for DEN-2 virus (K(d) ranging from 98 to 171 pM). Treatment of cell surfaces with certain glycosidases significantly reduced virus binding to mammalian cell lines, but not to the insect cell lines examined. Furthermore, heparin was found to compete with mammalian cell receptors for binding to DEN-2 virus and to inhibit viral infection of mammalian cells, but heparin had no effect on viral binding to or infection of insect cells. These results confirm previous reports suggesting that DEN-2 virus receptors on mammalian cell lines are different from those on insect cell lines.

Animals↗
PubMedDate not supplied

In vivo mass production in the cabbage moth (Mamestra brassicae) of a heterologous (Panolis) and a homologous (Mamestra) nuclear polyhedrosis virus.

In preparation for field trials, a nuclear polyhedrosis virus (NPV) of the pine beauty moth, Panolis flammea, was mass produced in vivo in an alternative (heterologous) host, the cabbage moth, Mamestra brassicae. Simultaneously, Mamestra NPV was also produced in M. brassicae. This homologous NPV/host system was a consistently more efficient production system than the heterologous one. Evidence is presented that increased humidity may improve levels of infection and, therefore, yield achieved. Full purification of the resultant product through a sucrose gradient resulted in a loss of virus of about 30%.

Animals↗
PubMed1987

Proteins induced by Boolarra virus in Drosophila melanogaster line 1 cells.

In Boolarra virus infected Drosophila line 1 cells five virus-induced proteins were readily detected 20 h postinfection. These were A (103,500), alpha (46,000), beta (40,000), B (9,600), and gamma (6,600) and are similar in molecular weight to those induced in vivo by other nodaviruses. The addition of actinomycin D for the 1st h before the labeling period resulted in the production of all five proteins; but when actinomycin D was introduced for the entire labeling period, the synthesis of the products of RNA1 was greatly reduced. Host functions inhibited by actinomycin D may play a major role in the replication of this virus.

Animals↗