Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “INSEMINATION”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 955 records · Page 53Linked to original sources
PubMed2006

Effects of coitus and the artificial insemination of different volumes of fresh semen on uterine contractions in mares.

Uterine contractions may play an important role in the transportation of spermatozoa towards the site of fertilisation in the oviduct of mares. M-mode ultrasound was used to measure the number, amplitude and duration of uterine contractions in each uterine horn and the uterine body of oestrous mares for four minutes before and four minutes after either coitus, or the artificial insemination of either 80.0 ml of fresh semen or 10.0 ml of fresh semen. The direction of the uterine contractions in each uterine horn and the uterine body was measured before and after coitus. Coitus and the insemination of 80.0 ml semen significantly increased the total number, mean amplitude and mean duration of contractions in all parts of the uterus. The insemination of 10.0 ml of semen did not affect the total number or the mean duration of contractions in the uterine horns. Their mean amplitude was increased, but largely owing to the results from one mare; it also did not affect the contractions in the uterine body. There was no significant difference between the percentage of contractions moving in a cervicotubal or tubocervical direction after coitus in any part of the uterus examined.

Animals↗
PubMed1991

[Early ultrasonic diagnosis of twins after insemination and contralateral embryo transfer in cattle and the results of calving].

In order to test the efficacy of ultrasonic diagnosis of early twin pregnancy, 20 dairy cows were inseminated, and 4-8 days later a seven-day old embryo was additionally transferred into the uterine horn contralateral to the site of ovulation. Sixteen of 20 cows became pregnant (80%). In 7 of 16 animals a twin pregnancy resulted (44%). Out of eight single pregnancies five (62.5%) followed insemination, and three (37.5%) followed egg transfer. Sixteen pregnant animals were examined ultrasonically. In 7 cases it was possible to estimate a twin pregnancy on the 41st day. This diagnosis was verified on parturition. The ultrasonically diagnosed side of pregnant horn in the singles was correct in 7 of 8 cases, identified by the genetical different origin of the offspring from artificial insemination or egg transfer.

Animals↗
PubMedDate not supplied

Artificial insemination with fresh donor semen.

One hundred and thirty-four patients received artificial insemination with fresh donor semen (AID). Using the life-table method of analysis, 72 pregnancies occurred, yielding a crude conception rate of 53.7%. The 134 women received an average of three inseminations per cycle for a total of 1,282 inseminations near the day of ovulation. The majority of pregnancies occurred by the seventh cycle. Forty-two pregnancies were achieved using a single donor; 30 pregnancies required changing the donor once, twice, or more. Among 42 patients who subsequently were treated with clomiphene citrate, 16 conceived. Two of these patients became pregnant after receiving hMG and hCG additionally. The life-table analysis of cumulative pregnancy rates following infertility therapy provides a relatively simple, powerful, and reliable tool for evaluating the effectiveness of therapy and its benefits. Patients should be advised that their chances of conception with AID approach that of normal fertile couples, provided that these patients persist in their treatment.

Adult↗
PubMed1991

Intraperitoneal insemination in the treatment of male and unexplained infertility.

For couples with unexplained or male infertility, intraperitoneal (IP) insemination in induced cycles is a method that increases the chance of fertilization. Seventy-seven couples with male subfertility were subjected to 120 IP insemination cycles and 31 with unexplained sterility to 44 cycles. As a consequence of the treatment, 23 pregnancies were obtained, with pregnancy rates of 23% per cycle and 32% per patient for unexplained infertility and 11% and 17% for male subfertility. Pregnancy loss rate was quite elevated: 9 clinical abortions and 1 ectopic. Intraperitoneal insemination appears to be a relatively noninvasive way to deal with unexplained or male factor infertility, well worth trying before moving on to more invasive and costly approaches, such as gamete intrafallopian transfer or in vitro fertilization.

Abortion, Spontaneous↗
PubMed1990

[Experience with intrauterine insemination].

Intrauterine insemination (IUI) was performed on sixty eight couples with a total of 126 cycles between July 1984 and December 1986. 14 pregnancies (20.5% per couple, 11.1% per cycle) were obtained with this therapeutical approach. In 39 couples a male factor was cause of infertility. In this group six pregnancies were obtained in 71 treatment cycles (15.1% per couple, 8.4% per cycle). Sterility due to a cervical factor was treated through IUI in 18 couples during 34 cycles. Six pregnancies (33.3% per couple 17.6% per cycle) were obtained in this group. Unexplained infertility found in 11 couples was treated with IUI in 21 cycles; two pregnancies (18.2% per couple, 9.5% per cycle) were obtained. Most pregnancies in all causes of infertility were obtained in the two first insemination cycles and no pregnancy was obtained after four cycles. Active ovulation induction was performed in the majority of the cases and only one insemination was usually accomplished. The only predictive semen parameter statistically significant in terms of pregnancy in masculine factor, was the total amount of progressive spermatozoa recovered after the washing procedure. In other words, pregnancy in more probable when less spermatozoa are lost during the procedure.

Adult↗
PubMed1989

[Fertility results after insemination of a moderate count of cock sperm, diluted in Blumberger cock sperm diluent and homologous seminal plasma].

Incomplete studies into artificial insemination of White Leghorn hens appear to suggest that the generally common number of cock spermatozoa per insemination can be considerably reduced without adverse consequences for reproduction results. Fertilisation rates between 89.4 and 94.9% were recorded from inseminations of as little as 20 x 10(6) spermatozoa, based on tenfold dilution of ejaculate collections in Blumberg Cock Sperma Diluent (BCSD) or homologous seminal plasma or differentiated combinations of both. Addition of increasing amounts of seminal plasma, however, caused decline in hatching rates as a result of rising embryonic mortality. Best suitability was recorded from a diluent combination of 75% of BCSD with 25% of seminal plasma, with the hatching rate being 85.0%.

Animals↗
PubMed1989

Insemination of beagle bitches with frozen semen.

Behaviour, vaginal cytology and plasma progesterone concentrations were monitored during oestrus in a group of 6 beagle bitches before intrauterine insemination with frozen semen. At intervals after insemination, the reproductive tracts were examined for evidence of conception, which occurred in 5 of the bitches. One pregnant bitch had received treatment with stilboestrol before insemination.

Animals↗
PubMed1989

[Forensic medicine aspects of surrogate mothers and artificial insemination].

Artificial insemination solves the problem of childlessness in a way never before thought possible. There is now often a lack of guiding principles in law, and new ethical questions have arisen. It would appear that homologous insemination is the simplest. This is recognized by the church; by doctors, even for unmarried couples. Heterological insemination, on the other hand, is only rarely acceptable to married couples, and then subject to the limits of generation, the avoidance of half-brothers and sisters, and gamete mistakes, as well as any commercialization. The medical standpoint and other legal developments must be reconciled with these factors; particularly the possibility of cancelling the anonymity of the sperm donor and the right to appeal of the social father. As a general principle, the ruling premise should be to place the burden of risk on the parents. With in-vitro fertilization, the existence of surplus embryos is particularly problematic. It is becoming more and more common to regard life as commencing in the zygote and not in the nidation. Can the scientific experiments permitted under the new professional regulations-even with more complicated requirements-be reconciled with this? Consensus can be reached in rejecting surrogate motherhood, since the danger of commercialization is too great, and propagation possibilities are being opened up which, to our present understanding, are irresponsible.

Expert Testimony↗
PubMed1979

Synchronization of oestus and timed insemination of mares.

Oestrus was synchronized in 116 mares by means of an i.m. injection of prostaglandin F-2 alpha (Day 0) and of fluprostenol (a PG analogue) on Day 16. Mares were then randomly divided into three groups. Group A mares (N = 30) were given 2500 i.u. hCG I.M. ON Day 20 and artificially inseminated on Day 21 without detection of oestrus. Group B mares (N = 32) were given 2500 i.u. hCG i.m. on Day 20 and inseminated on Days 21 and 23, also without oestrus detection. Group C mares (N = 54) were teased on Days 18, 19, 21, 23 and 25 and inseminated on Days 19, 21, 23 and 25 while they were in oestrus. Semen was collected by artificial vagina from 3 stallions. One-third of the mares in each group were assigned to each stallion at random. The gel-free fraction was divided equally among the mares, and used within 1 h of collection. Pregnancy rates at about 60 days of gestation were not significantly different. A high rate of synchronization of oestrus (80%) was attained within 48 h of treatment with fluprostenol.

Animals↗
PubMed1986

Temporal abundance and percent insemination of newly-emerged adult female Psorophora columbiae near the larval habitat.

The temporal abundance and percent insemination of newly-emerged adult female Psorophora columbiae were investigated in riceland sites near Anahuac, TX. Pre- and postmating nights were characterized by low numbers of females and little mating activity (no significant increase in percent insemination) occurring in the study site. Mating nights were characterized by high female abundance in the study site before sunset then steadily decreased until a stable, low level of abundance was reached approximately 2 to 2.5 hr after sunset. On mating nights, percent insemination of female mosquitoes in the study site remained low until sunset, after which it steadily increased until a maximum was reached about 2 hr after sunset. On each mating night, approximately 2 to 2.5 hr after sunset, a mass exodus from the site by primarily mated females with some males was observed.

Agriculture↗
PubMed1985

[Markers of the periovulatory phase with an eye toward insemination: E2/LH correlations].

We are investigating the most practical parameters i.e. reliable and with a rapid response, which allow the time of insemination to be determined in women undergoing stimulation because of anovulation. We have already shown the advantage of rapid radio-immunological determinations of 17 beta-oestradiol (E2) to induce the releasing action of chorionic gonadotrophic hormone (hCG). We have carried out our investigations directed towards the rapid-immunological determination of luteotrophic hormone (LH), and have recorded a good temporal correlation between E2 and LH, the rise of E2 preceding by some hours that of LH, and the two summits succeeding each other. Inseminations are followed by fertilization in women who present with the following: a mean oestradiolaemia of about 500 pg/ml in the second part of the follicular phase, maintained after interruption of HMG administration (alone or after clomiphene citrate), which decreases only slowly after administration of hCG; a level of LH increasing from 9-14 ng/ml (mean) in the last two days preceding the summit of LH; the level of LH multiplied at least by 2 after the administration of hCG, whatever the mode of stimulation. Clomiphene citrate, not provoking a constant ovulatory discharge, does not dispense with the administration of hCG in the majority of cases. These considerations result from the study of a group of 22 anovulatory women, in whom stimulation followed by determination of E2 and LH, under echographic control, have allowed 12 pregnancies to be obtained (7 by clomiphene citrate + HMG + hCG; 5 by HMG + hCG), decreasing the mean number of inseminated cycles to 3 per woman thus studied.

Adult↗
PubMed1985

[Outlook for the programmed insemination of sheep in the anestrual season].

Studied were certain methods for programming the estrus and fixing the optimal term for the insemination of sheep. A record was kept of the changes taking place in the genital apparatus in various periods of time following the use of hormonal preparations during the anestral period. The fertility of sheep was also taken into consideration both after the first treatment with serum gonadotropin and after the two-fold programmed insemination at a 16-day interval with regard to the total fertility. It was shown that the optimal time for programmed insemination of sheep was between the 36th and the 56th hour after the three-fold injection with Prolosan at 12-hour intervals so far as periods other than the breeding season were concerned. Fertility at two-fold injection rated at 121 per cent, and conception--at 78.2 per cent.

Anestrus↗
PubMed1984

[Traumatic insemination of Oxyuroidea of dermoptera and leporidae. Comparative morphological study. Considerations about phylogenesis].

A morphological study of traumatic insemination in the genera Auchenacantha, a parasite of Dermoptera, and Passalurus, a parasite of Rabbits, revealed that the same phenomenon appeared twice in distinct lineages. In the Auchenacantha, adult females are inseminated in the vagina vera modified into a spermatic pouch. In the Passalurus insemination occurs in the ventral hypodermal field. Adult males probably mate with the female larval, stage four.

Animals↗
PubMed1995

The use of zona-free aged unfertilized human oocytes as a predictor for successful subzonal insemination.

OBJECTIVE: To examine the reliability of the zona-free aged (1 day old) human unfertilized oocyte sperm penetration assay for assessing sperm fertilizing ability and to determine the predictive value of this assay for subsequent subzonal insemination (SUZI) outcomes. DESIGN: A total of 253 unfertilized oocytes from total fertilization failure patients and from good fertilization rate (> 70%) patients in standard IVF were inseminated with donors' spermatozoa, and penetration rates were compared in each group. Two hundred seventy-two unfertilized oocytes from total fertilization failure, poor fertilization (< 30%), and normal fertilization (> 30%) were inseminated with husbands' spermatozoa, and penetration rates were compared between the three groups. In 29 patients, the results of the zona-free assay performed in previous IVF were compared with the fertilization rates of subsequent SUZI. RESULTS: In the zona-free assay using donors' spermatozoa, there was no difference in penetration rates between the two groups (109/122, 89.3% versus 114/131, 87.0%). Penetration rates using partners' spermatozoa were positively correlated with fertilization rates in standard IVF (total fertilization failure 34/75, 45.3%; poor fertilization 56/77, 72.7%; normal fertilization 108/120, 90.0%). There was a significant difference in fertilization rates after SUZI between the patients with negative and positive penetration in zona-free assay (4/53, 7.5%, versus 54/174, 31.0%). CONCLUSION: The zona-free human oocyte assay may primarily reflect sperm fertilizing ability. This asssay also could be a reliable predictor for subsequent SUZI outcome.

Cellular Senescence↗
PubMed1995

[Homologous intrauterine insemination as a first choice treatment of infertility].

Intrauterine insemination with husband's Percoll preparated sperm was performed in 179 couples in a total of 440 treatment cycles. A total of 60 pregnancies was obtained. The pregnancy and delivery rate was 13.6% and 9.3% per insemination cycle, respectively. The cumulative probabilities of pregnancy and delivery were respectively 42% and 31% after three cycles. There was a significantly higher pregnancy rate when the number of follicles was more than three and a trend towards more pregnancies when the number of spermatozoa was increased. The study shows that this easy and non-invasive treatment gives acceptable results. We recommend an ideal maximum of three treatment cycles, and at least 1-2 million spermatozoa for each insemination.

Adult↗
PubMed1995

[Family secrets exemplified by heterologous insemination].

Potential consequences of intrafamilial concealment are discussed in the context of coping with the stigmatizing diagnosis of male infertility and its treatment by artificial insemination by donor. First results of a longitudinal study are presented. A 5-year follow-up was obtained from 40 out of 57 couples initially interviewed. 34 of the couples had kept donor insemination secret even towards their families. 10 of them considered themselves oppressed by the secrecy, especially at the time of insemination. Only two out of 26 successful couples intended to reveal details of conception to the child. These findings suggest that information of the children can follow different strategies. Children should be acquainted with details of their conception if friends or relatives of the parents are informed, in order to reduce the risk of the traumatic event of information by others. In all other cases the decision to inform children or not is to be left in the hands of the parents. Even though maximum candidness is considered desirable today, the "cathartic" revelation of family secrets can be as destructive as their conservation.

Adaptation, Psychological↗
PubMed1993

[Familial relations in connection with donor insemination. Psychological effects of secretiveness].

In Norway approximately 150 children are born every year as a result of artificial insemination. In 1987 an Act was passed regulating this practice. Hospital gynaecologists are given the authority to implement this Act, and the responsibility for doing so. With the current practice of artificial insemination, two types of secrets evolve: The anonymity of the donor, as required by law, will prevent the child from ever identifying its biological father. The secrecy of conception, as recommended by experienced gynaecologists as the best method of protecting the child and the parents, will continuously present the family with unresolved questions that may create problems within the family. It is assumed that it would present an undue burden on the child not to be able to identify its biological father. As a family psychiatrist the author has discussed the secrecy issue with nine couples with children conceived by artificial insemination using donor semen, and another eight couples that were offered this treatment. A common denominator for these couples turned out to be the problem of living their entire life not knowing the origin of their child. Most of the couples are afraid that the secret could be divulged during a family quarrel. Invisible alliances could develop between members of the family. In the long run, honesty about the conception reduces the mental strain on the family. Family therapists will generally advise against secrecy in these matters and will help parents choosing alternative ways of coping with the problems.

Adult↗
PubMed1993

The effect of induced standing reflex, cervical stimulation and insemination on intraluminal pressure variations in the isthmus of the oviduct in unrestrained gilts.

Four gilts were each equipped with 2 ultra-miniature pressure sensors, placed at 2 different points along the same isthmus of the oviduct, on the morning of the first day of standing oestrus (Day 1). Intraluminal pressure recordings were started the same afternoon. After an initial recording period, intraluminal pressure was recorded while the gilts showed standing oestrus and during cervical stimulation followed by insemination with either 100 ml saline or 100 ml boar semen. Monitoring of the pressure variations in the isthmus was continued for up to 6.5 h after the last insemination. Blood samples for monitoring oestradiol-17 beta, progesterone and 15-ketodihydroprostaglandin F2 alpha were collected before and after each manipulation of the gilt and every 30 min during the rest of the test period. None of the above manipulations had any consistent effect on the intraluminal pressure in the porcine isthmus, although, a clear 15-ketodihydroprostaglandin F2 alpha peak could be seen after insemination with boar semen.

Animals↗