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Clinical application of the forearm island skin and fascia flap with posterior interosseous artery pedicle--report of 15 cases.

The present paper reported 15 cases, in which skin and fascia forearm island flap with posterior interosseous artery pedicle was applied. It was found that this flap has the advantages of stable vessels, abundance in blood supply and good circulation, without sacrificing the main arteries of the forearm. As a result, it is an ideal method for repair and reconstruction of the hand and forearm.

Adolescent↗

Pubovaginal or vicryl mesh rectus fascia sling in intrinsic sphincter deficiency.

The aim of this study was to compare the pubovaginal sling with a new Vicryl mesh rectus fascia (VMRF) sling in the surgical treatment of low urethral pressure genuine stress incontinence. Fifty-one consecutive women who had a VMRF (n = 27) or a pubovaginal sling (n = 24) procedure between March 1995 and December 1997 were evaluated. The patient-determined subjective success rate of the VMRF sling (85%) was significantly higher than that of the pubovaginal sling (58%) in women with low urethral pressure stress incontinence (P=0.03). The objective success rates following the VMRF and the pubovaginal sling were 52% and 50%, respectively. The prevalence of postoperative symptomatic voiding dysfunction and de novo detrusor instability was 7% after the VMRF sling and 25% following the pubovaginal sling (P = 0.08). The VMRF sling had a higher patient-determined success rate and a lower complication rate than the pubovaginal sling, and should be considered in the surgical management of women with low urethral pressure stress incontinence.

Adult↗

A stratigraphic approach to the superficial musculoaponeurotic system and its anatomic correlation with the superficial fascia.

The superficial musculoaponeurotic system (SMAS) is not included in the International Anatomic Terminology, although it is a fundamental anatomical structure in plastic surgeons. In the literature, the concept of the SMAS is not clear, leading to repercussions in the treatment of the SMAS via the various techniques for rhytidoplasty. This review article, in its aim to conceptualize the SMAS, has as a referential parameter the basic stratigraphic pattern of the human body construction. A systematic review of the literature was performed through Medline, ISI-Web of Science, and Lilacs databases as well as through classical treatises of anatomy for analyses of the stratigraphic principle of SMAS and its relationship to the fascia. In fact, SMAS, an anatomic entity, is part of this construction model. The stratigraphic approach followed in this article to describe the SMAS is novel in the literature and allows for a unified anatomic understanding of SMAS for the scientific community.

Adipose Tissue↗

Toldt's fascia flap: a new technique for repairing large diaphragmatic hernias.

The most popular techniques for repairing large diaphragmatic defects involve the use of synthetic patches. We present an alternative approach using living tissue. We reviewed our cases of congenital diaphragmatic hernia (CDH) diagnosed within the first 24 h of life from 1991 to 2003. Toldt's fascia (TF) flap was used to repair defects that were too large to repair primarily even though the anterior rim of the diaphragm was present. After confirming that a small medial muscle remnant of the diaphragm was present, its mesothelial covering was incised, and the incision was extended to the TF far enough to create a flap sufficiently large to repair the defect. The TF flap, consisting of the small medial muscle remnant, TF, peritoneum, and retroperitoneal connective tissue, was mobilized carefully from the ipsilateral kidney and adrenal gland, and the repair completed with interrupted sutures using nonabsorbable material. We used this TF flap approach in seven of 43 patients with CDH. Two had right-sided CDH. Six survived. The mean size of the diaphragmatic defects in the seven TF flap cases was 5.43+/-0.53 x 3.86+/-1.07 cm, which was significantly larger than the defects in direct primary repair cases (3.40+/-0.77 x 2.03+/-0.59 cm) (p<0.01). The six survivors had good outcomes, and none of them have had recurrence of herniation or required any additional surgical intervention (mean follow-up period: 4.7 years). To the best of our knowledge, this is the first report of TF being used to repair large diaphragmatic hernias. Our technique is simple and has proven to be reliable for durable restoration of the diaphragm, suggesting that it could reduce the dependence on synthetic patch repair, which is associated with certain long-term complications.

Abdominal Muscles↗

Successful treatment of an extra-abdominal fibromatosis (desmoid tumor) arising from the prevertebral fascia of the neck.

We describe the successful treatment of a fibromatosis (desmoid tumor) arising from the prevertebral fascia of the neck. Total resection with wide margins is reportedly the best treatment for this kind of tumor. However, the anatomy of the head and neck makes such resection difficult. In this case, we were unable to completely remove the tumor because it was large and located close to the cervical vertebrae, common carotid artery and internal jugular vein. Incomplete resection is known to result in higher tumor recurrence than complete resection. In addition, the recurrence or progression of a tumor in the head or neck region is known to cause mortality by compression of the airway or major blood vessels. On the basis of reports that irradiation is effective treatment for this kind of tumor, we administered 30-Gy irradiation to the affected area. This therapy was very effective and no sign of recurrence was seen for 2 years after irradiation. We found that function-sparing resection plus postoperative radiotherapy is an effective treatment for advanced fibromatosis in the head and neck regions with proximity to or involvement with vital structures.

Desmoid Tumors↗

Combined fascia and mesh repair of incisional hernias.

Incisional hernia is a relatively common complication following abdominal operations. Due to high recurrence rates following primary suture, a prosthetic mesh is now usually used to repair the hernia. Several different types of repair are described. A combined fascia and mesh technique avoids opening the peritoneal cavity and potential damage to bowel. This study describes results of this technique. Consecutive patients undergoing the operation were contacted to assess freedom from recurrence and satisfaction with results. Some 34 patients underwent 36 incisional repairs. Of the 34 patients 21 were men and 13 were women with a median age of 69 years (range 31-86 years). The causative incisions were midline (30), Kochers (2), Pfannesteil (2) and transverse (2). The median time spent in hospital was three nights (range 1-14 days). Two patients developed seromas, another complained of pain soon after operation, one patient developed a superficial wound infection another developed a tender drain site which required exploration. One patient with significant co-morbidity died 13 days postoperatively from multiorgan failure. Freedom from recurrence of the hernia was 91% at 5 years. However patient survival was only 70% at 5 years. This technique is associated with low postoperative morbidity and a low recurrence rate. Patients presenting with incisional hernias have a poor long-term prognosis which needs to be borne in mind when planning intervention.

Adult↗

A periosteal-temporalis fascia pedicle flap for repairing impending ocular perforations and extruding keratoprostheses.

We repaired four eyes in four patients with impending perforation using a vascular periosteal-temporalis fascia pedicle flap continuous to the periorbita of the orbital wall. Two eyes with extruding keratoprostheses, one eye with severe ocular surface dysfunction and a perforated, infected keratoplasty, and one eye with scleral ectasia were successfully reconstructed. Excellent visual acuity (20/25) was attained in one eye and functional visual acuity (20/100) was maintained in another. Structural integrity was maintained in all eyes. A vascularized flap was an excellent source of tissue for reconstructing eyes with impending perforation and scleral melting.

Adolescent↗

Synaptic enhancement in fascia dentata: cooperativity among coactive afferents.

Evidence is presented that long-lasting enhancement of perforant path synapses following high-frequency activity is a cooperative process requiring coactivity of a considerable number of fibers. This was demonstrated in two ways: (1) A threshold stimulus intensity during high-frequency stimulation was required before any enhancement was observed. This threshold was considerably above the stimulus threshold for observing a minimal synaptic response in the fascia dentata. Above the enhancement threshold, the amount of enhancement increased as the intensity of high-frequency stimulation increased. (2) Concurrent high-frequency activation of fibres of the medial and lateral perforant path produced enhancement at intensities where identical but independent stimulation of the two pathways either failed or was less effective. Discharge of the granule cells during the high-frequency stimulation was independently manipulated and found not to be physically coupled to the enhancement generating mechanism.

Afferent Pathways↗

Development of high affinity choline uptake and associated acetylcholine synthesis in the rat fascia dentata.

The ontogenic development of hemicholinium-sensitive, high affinity choline uptake and the synthesis of acetylcholine from exogenous choline have been studied in particulate preparations of the rat fascia dentata. Between 6 days of age and adulthood the rate of high affinity choline uptake increases 3-fold, when expressed with respect to protein, and 125-fold, when expressed independently of protein. This process develops most rapidly during the period around 16-17 days of age, similar to the ontogenesis of choline acetyltransferase activity. This observation supports the idea that cholinergic septohippocampal boutons develop mainly at this time. Unlike choline acetyltransferase activity, the velocity of high affinity choline uptake increases to as much as 161% of the adult value at about 30 days of age. It is suggested that at 25-31 days of age a relatively high endogenous septohippocampal firing rate increases the rate of choline uptake. At 6 days of age we detected no synthesis of acetylcholine from the accumulated choline. Uptake-synthesis coupling develops mainly between 6 and 13 days of age, earlier than any other presynaptic cholinergic property. Acetylcholine synthesis from exogenous choline develops in paralled with high affinity choline uptake, but developmental increases in uptake velocity result in comparable increases in synthesis rate only after a delay of several days. Some limiting factor other than choline acetyltransferase activity appears to link the accumulation of exogenous choline to acetylcholine synthesis during development.

Acetylcholine↗

Evidence for two physiologically distinct perforant pathways to the fascia dentata.

The effects of paired and brief trains of stimuli were compared in the medial and lateral components of the perforant pathway to the rat fascia dentata, both in vivo, and in the vitro transverse hippocampal slice. In the intact preparation a sharp transition in response properties occurred at the midpoint of the medial to lateral range of input. In vitro this transition was found to correspond exactly to the transition in Timm stainability which characterizes the border of the medial and lateral termination zones in the outer 2/3 of the molecular layer. These data indicate that there are two physiologically distinct subdivisions of the perforant path. Evidence is presented that the interpathway differences are at least partly due to differential quantal contents of the EPSP under resting conditions.

Afferent Pathways↗

Septal modulation of the population spike in the fascia dentata produced by perforant path stimulation in the rat.

Electrical stimulation of the perforant path, which originates in the entorhinal cortex, produces a characteristic excitatory postsynaptic field potential (extracellular EPSP) which can be recorded in the fascia dentata. This evoked response may include a population spike, if stimulation is sufficient. In the anaesthetized rat, stimulation of the medial septum, when paired with perforant path stimulation, was found to augment the population spike component of the evoked field potential. Stimulation of the septum alone produced no apparent field potential. The augmentation effect was found to have a rapid onset (4 ms), which is sufficient for the participation of interneurons, and a relatively long time course (150 ms). Presynaptic mechanisms of facilitation were ruled out as there was no concurrent alteration of the extracellular EPSP. A change in population spike threshold, compatible with a postsynaptic mechanism, was observed and some possible models of action discussed. Augmentation survived depletion of hippocampal norepinephrine caused by injections of 6-hydroxydopamine into the dorsal noradrenergic bundle, indicating that the facilitation was not due to an activation of the ascending noradrenergic fibres of passage originating from the locus coeruleus. The cholinergic septo-hippocampal pathway was ruled out as a likely candidate for the modulation as the augmentation survived injections of the muscarinic antagonists atropine and scopolamine and the nicotinic antagonists tubocurarine and dihydro-beta-erythroidine. A relationship between the septal modulation and hippocampal theta was suggested.

Animals↗

Ectopic granule cells of hilus fasciae dentatae projecting to the ipsilateral regio inferior of the rat hippocampus.

Retrograde fluorescent tracing techniques were used in a search of projections from the hilus of fascia dentata to the Ammon's horn. We found that up to 5% of the neurons in the hilus project to the ipsilateral regio inferior. The projection originates from cells that morphologically resemble ectopic granule cells and terminates in the mossy fiber layer. We found no evidence for a projection from the hilus to the ipsilateral regio superior or the contralateral Ammon's horn.

Animals↗

Evidence for differential localization of two binding sites for L-[3H]glutamate in rat fascia dentata.

Two binding sites for L-[3H]glutamate were tentatively localized in rat fascia dentata by determining the effects of selective lesions on specific binding. Both destruction of dentate granule cells with colchicine and ablation of the ipsilateral entorhinal cortex markedly reduced radioligand binding to a quisqualate-sensitive site (GLU A), but only the entorhinal lesion significantly reduced binding to a site that is less sensitive to quisqualate (GLU B). These results suggest that GLU A binding sites are localized mainly on the dentate granule cells, whereas GLU B binding sites are localized, in part, on the perforant path fibers, but not on granule cells.

Animals↗

Calcium distribution in dendritic spines of the dentate fascia varies with age.

Calcium distribution in dendritic spines of the dentate fascia was studied as a function of age with the oxalate-pyroantimonate precipitation technique. In postnatal ages P3, P9, P24 and P30 spines were analyzed as to the presence of the spine apparatus (SA) and as to the presence of Ca2+ deposits within the SA and within the spine cytoplasm. The percentage of spines with SA-containing precipitates declined significantly between P3 and P24. Conversely, the percentage of spines with precipitates in the spine cytoplasm was significantly increased by P24. In the absence of an SA loss, this result suggests an age-related decrease in the Ca2+-sequestering capacity by the SA. These parameters were improved by P30 so that they approximated the values of P3. Such a seeming amelioration could be attributed to the fact that the mortality rate in rats sharply increases by P24, so that animals surviving this age represent a selected population in which a compensatory growth of spines has occurred and has secured functionally valid connections.

Aging↗

Morphometric and dendritic analysis of fascia dentata granule cells in human aging and senile dementia.

In this study the cellular morphology in the human fascia dentata of 5 very old demented cases (4 Alzheimer's disease and 1 multi-infarct dementia patients) was compared with 5 (very) old controls cases. The postmortem delay in fixation was for all cases within 3.5 h. In the demented group, a significant reduction in thickness of the molecular layer, density of dendritic spines in the middle third of the molecular layer and total dendritic length (+/- 30%) was found. The number of dendritic segments, indicative of the branching frequency showed no difference. In both the control and the demented group, three-quarters of all dendritic bifurcations of granule cells occurred in the inner third of the molecular layer in which the commissural and associational fibers terminate. The size of the dendrites in the demented group could be the result of at least 3 independent processes: a regressive change due to partial denervation of the outer two-thirds of the molecular layer as axons from the perforant pathway are lost; a dendritic regrowth in response to sprouting of the commissural-associational fiber systems and septal afferents, which is presumed to occur in response to degeneration of perforant path axons; a dendritic regrowth in response to the loss of the dendrites of neighboring cells which have died. Analysis of our material suggests that dendritic degeneration is the predominant factor in the demented group.

Aged↗

Changes in the frequency of basket cells in the dentate fascia following chronic ethanol administration in mice.

The frequency of basket cells in the granule cell layer of the dentate fascia of Short Sleep (SS) and Long Sleep (LS) mice was determined following 3 months of ethanol exposure. These mice were bred for their differential susceptibility to the narcotic effects of acute doses of ethanol. The ethanol-insensitive SS mice were unaffected by the treatment while the ethanol-sensitive LS mice that received ethanol showed a significant decrease in basket cell frequency over their control group counterparts. These basket cells are thought to control the tonic level of activity of the granule cells. Thus, a decrease in basket cell frequency might lead to higher granule cell activity following chronic ethanol exposure. This effect could counteract the assumed stronger depressant effect of ethanol in the relatively ethanol-sensitive LS mice.

Animals↗

Spreading depression-like hypoxic depolarization in CA1 and fascia dentata of hippocampal slices: relationship to selective vulnerability.

Hippocampal tissue slices were made hypoxic for 4-10 min and then reoxygenated for 60-120 min. Postsynaptic evoked potentials were recorded and extracellular DC potential was monitored continuously in stratum (st.) pyramidale of CA1 and st. granulosum of fascia dentata (FD). In some preparations extracellular potassium ([K+]o) and calcium ([Ca2+]o) were also recorded in both regions. Postsynaptic responses disappeared sooner during hypoxia and were less likely to recover upon reoxygenation in CA1 than in FD. The CA1 region exhibited a spreading depression (SD)-like response to hypoxia more often than did FD. When both regions showed SD-like depolarization, voltage shift and elevation of [K+]o were of greater magnitude and shorter latency in CA1. The probability of posthypoxic recovery of synaptic transmission was inversely related to the time spent in the SD-like state in both CA1 and FD. We conclude that the selective vulnerability of CA1 neurons to hypoxic and ischemic damage may be due, at least in part, to the region's propensity to undergo prolonged and severe SD-like depolarization.

Action Potentials↗

Entorhinal fibers form synaptic contacts on parvalbumin-immunoreactive neurons in the rat fascia dentata.

The entorhinal cortex gives rise to a dense projection to the outer two-thirds of the dentate molecular layer. The main target neurons are the granule cells. This study demonstrates that entorhinal fibers labeled by anterograde degeneration also terminate on parvalbumin-containing non-granule cells in the rat fascia dentata. Since the calcium-binding protein parvalbumin was recently found coexistent with gamma-aminobutyric acid in inhibitory hippocampal neurons, the described connection provides evidence for a role of entorhinal fibers in feed-forward inhibition of the granule cells.

Animals↗