[Penetration of I-131-labelled bacterial antigens through intact enamel and dentin in vivo and in vitro].
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The breakthrough times and permeation rates of two commonly used allergenic components in dentin bonding agents or resins, HEMA and TEGDMA, were measured for 5 types of latex gloves and 5 types of nitrile gloves. In addition, the breakthrough times and permeation rates for the gloves were measured for HEMA and TEGDMA when diluted with either ethanol or acetone-solvents often appearing in dentin bonding agents. The mean breakthrough times for the 5 latex gloves for HEMA and TEGDMA, concentrated, diluted in ethanol, or diluted in acetone, were 4.9, 4.8, and 2.8 min, respectively. For the 5 nitrile gloves the equivalent breakthrough times were 15.7, 9.9, and 2.8 min, respectively. There were great variations between the various gloves, and 1 nitrile glove showed a breakthrough time of 28-30 min when tested with concentrated HEMA and TEGDMA. Compared to latex gloves, nitrile gloves have a longer-lasting protection against skin contamination with methacrylates in the absence of solvents. The longer protection was reduced or not present for methacrylates diluted in organic solvents, especially acetone. In addition, the nitrile gloves showed fairly high permeation rates in the presence of this solvent. The results indicate that latex and nitrile gloves only give a limited protection against allergenic methacrylates in dentin bonding agents when they contain acetone.
OBJECTIVE: Dentine hypersensitivity may occur due to loss of covering enamel and/or loss of cementum after gingival recession, resulting in exposure of cervical dentine and patency of dentinal tubules. The effect of thermally cooled pulsed neodymium:yttrium-aluminium-garnet (Nd:YAG) laser on the permeability and structural appearance of the root canal wall was investigated in vitro. The aim of this study was to compare the occluding effect of Nd:YAG laser with that of sensodyne on exposed dentine. MATERIAL AND METHODS: Forty freshly extracted human maxillary anterior teeth were collected at random. Root canal obturations were performed using master apical file no. 60 k-type for obturated teeth. All teeth were stored in 100% humidity at 37 degrees C for 48h to allow time for the sealer to set. Dentine of 35 teeth was exposed by removing the cementum, and the remaining five teeth represented a negative control group. Specimens were examined using stereomicroscopy to show the surface topography of the dentine substrate before and after treatment with Nd:YAG laser or sensodyne. RESULTS: A double-blind technique was used when taking measurements of ink penetration (in mm) for each group. Teeth treated by Nd:YAG laser showed the least ink penetration, followed by teeth treated by sensodyne. The positive control group demonstrated complete ink penetration and the negative control group showed no ink penetration. Stereomicroscopic examination of specimens treated with Nd:YAG laser showed deposition on the dentinal surface, and specimens treated with sensodyne showed partial deposition. Untreated specimens were free of deposition and the tubules remained patent. CONCLUSION: There is no significant difference in the occluding effect of Nd:YAG laser and sensodyne toothpaste. Both treatments have a promising effect on the reduction of permeation through exposed dentinal tubules. However, the occluding effect of Nd:YAG laser occurs within seconds whereas that of sensodyne takes at least 3 weeks.
This study tested the null hypothesis that there no difference between two-step and one-step self-etch adhesives in their compatibility with these composites. The microtensile bond strengths (microTBS) of two two-step systems (Clearfil SE Bond, Kuraray and Tyrian SPE/One-Step Plus, BISCO) were compared with two one-step systems (Xeno III, Dentsply DeTrey and Brush&Bond, Parkell) for their coupling to a dual-cured composite. Silver tracer penetration of the four adhesives bonded to a light-cured or a chemical-cured composite was examined using TEM. Significant differences in microTBSs between composite curing modes were seen only in the one-step adhesives. For one-step self-etch adhesives bonded to the chemical-cured composite, TEM revealed signs of frank composite uncoupling along the adhesive-composite interface, which may be attributed to the adverse chemical interaction between the acidic adhesive and the composite. In addition, "water trees" that represent channels of increased permeability with the polymerized adhesive layer were also observed in the one-step adhesives. Both features were absent along the resin-dentin interfaces when chemical-cured composites were coupled to the two-step self-etch adhesives.
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PURPOSE: To test the hypotheses that microtensile bond strengths of single-step, self-etch adhesives to hydrated dentin are adversely affected by delayed-activation of a light-cured composite, as well as the use of a chemical-cured composite. METHODS: Seven commercially available and one experimental single-step, self-etch adhesives were bonded to hydrated human dentin. A dual-cured composite was used and activated using: [1] the light-cured mode (base paste only-control), [2] the chemical-cured mode (base and catalyst pastes, in the dark); and [3] delayed light-activation (base syringe only, left on top of cured adhesives in the dark for 20 minutes before activation). After microtensile bond strength evaluation, fractured samples were examined using SEM. Intact, unstressed interfaces of [1] and [3] were examined with TEM. Microtensile bond strengths were also evaluated for selected adhesives bonded to dehydrated dentin and coupled with the composite activated by [2] or [3]. RESULTS: For all adhesives, bond strengths decreased significantly to bonded hydrated dentin when the composites were activated with [2] or [3] (P< 0.001). Whereas the hybrid layer-adhesive interface was intact, water-blisters that developed along the adhesive-composite interface in these two modes were responsible for the decline in bond strength. When bonded to dehydrated dentin, delayed light-activation of the light-cured composite did not result in compromised bond strength. The results of chemical-cured composites improved but were more variable and system-dependent.
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PURPOSE: The purpose of this study was to evaluate the microleakage and penetration ability of sealing materials applied under different conditions of contamination. METHODS: One hundred twenty extracted human molar teeth were randomly assigned to 12 groups. The treatment groups were defined by the combination of sealing materials (Concise; Optibond system; Optibond system plus Concise) and 4 surface conditions (no moisture and no saliva contamination; moisture contamination; dried saliva contamination; wet saliva contamination). Each tooth was subjected to thermal cycling (5,000 cycles at 5 degrees C-55 degrees C) with a dwell time of 30 seconds and dye immersion (5% methylene blue for 24 hours). Microleakage, penetration ability, and fissure types were examined after sectioning. Multiple regression analyses and the Tukey test were used for statistical analysis. RESULTS: Concise showed significantly less microleakage than the Optibond system (P<.031) when the procedures were performed under no contamination or moisture contamination. However, when Concise was applied on the wet saliva-contaminated surfaces, considerably higher microleakage and unfilled areas were found compared to the use of Optibond alone or Optibond with Concise (P<.001). CONCLUSIONS: When there is saliva contamination, the use of Optibond alone or with Concise is beneficial for decreasing microleakage and increasing the penetration ability of sealants.
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Intercellular junctions in the odontoblastic layer have been studied with a freeze-fracture technique. Children's tooth germs were fixed, sliced and demineralized. Samples of the pulpodentinal border were routinely prepared for freeze-fracture. Three kinds of intercellular junctions were detected between human odontoblast cell bodies: gap junctions, desmosomes and tight junctions. Numerous gap junctions are responsible for intercellular communication at different levels of the cell bodies. Focal tight junctions, parallel to the axis of the cell, and desmosomes are sites of cell-to-cell adhesion between lateral plasma membranes. At the distal end of the cell bodies, junctional complexes consist of zonular tight junctions and gap junctions. These zonular tight junctions, never before described between odontoblasts, contribute to the pseudo-epithelial organization of the odontoblastic layer. They constitute a predentin-pulp barrier, the permeability of which must be studied to establish their role in relation to dentin formation.