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PubMedDate not supplied

Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen: negative effect of disaccharides in vitrification solution.

Given that it has been possible to successfully cryopreserve human ovarian tissue by direct plunging into liquid nitrogen, this study was designed to establish the future direction to be taken in this line of research. Bovine oviductal epithelial fragments (as a tissue model) and large biopsy fragments (approximately 2.0 cubic mm) of human ovarian tissue were used for cryopreservation. Two protocols were tested: with permeable cryoprotectants (dimethyl sulphoxide, propylene glycol, glycerol) + egg yolk + sucrose or trehalose + a synthetic blocker of ice nucleation, Supercool X-1000; and using only permeable cryoprotectants (glycerol and ethylene glycol) + egg yolk + Supercool X-1000. The cryopreserved tissue specimens were subsequently thawed and the cryoprotectants removed by dilution in graded sucrose solutions. Both the dynamic growth and hormonal activity of the ovarian tissue pieces, vitrified using only permeable cryoprotectants, were greater than after vitrification in a mixture of permeable cryoprotectants and sucrose. Unlike the case for other reproductive tissue (spermatozoa, oocytes, embryos), these findings suggest that the cryopreservation of ovarian tissue by direct plunging into liquid nitrogen must be achieved by vitrification using only permeable cryoprotectants and agents that prevention ice formation.

Adult↗
PubMed2000

[The FAR-IR study of several mono and disaccharides and metal-sugar complexes in solid state].

FIR spectra of saccharides have many peaks. Each saccharide has its characteristic spectrum. The first, the second, the third, the fourth strongest peak appear at different regions(a, b, c, d) and the sequence (the first in a, the second in b, the third in c, the fourth in d) is characteristic. Due to the sequence in relative intensity is different, we use a simple method encoding four strong peaks to distinguish between different saccharides. We divide 500-100 cm-1 into ten ranges and encode each region as follows: 1(100-140 cm-1); 2(141-180 cm-1); 3(181-220 cm-1); 4(221-260 cm-1); 5(261-300 cm-1); 6(301-340 cm-1); 7(341-380 cm-1); 8(381-420 cm-1); 9(421-460 cm-1); 0(461-500 cm-1). For example, the codes of xylose are 6,538 and the codes of d-arabinose are 7 486. Each saccharide has a number. In this way, we can easily differentiate between saccharides. When the complexes have formed, there are broad peaks of M-O. The phenomena illustrate that FIR technique is an useful mean to determine the formation of the complexes and possibly detect their structures.

Disaccharides↗
PubMed1987

[Elimination of saccharose from the blood and its renal excretion following transfusion of buffy coat-free erythrocyte concentrates containing disaccharides].

The elimination of sucrose from the blood and its renal excretion was analysed in 108 patients after applying a total of 394 transfusion units (TE), resuspended, buffy-coat-free erythrocyte concentrates (EK) containing 23 mmol of sucrose per TE. In transfusing 3 TE even 90% of the sucrose were eliminated from the blood during the application time and up to 99% within 3 h, nearly 80% were excreted through the kidneys within 12 h. Elimination and excretion were delayed with impaired kidney function. With respect to intravasal elimination of sucrose bilaterally nephrectomized patients have to rely on hemodialysis. Side-effects of sucrose due to extended intravasal and interstitial duration could not be observed in those patients affected with decreased kidney efficiency and after massive transfusions.

Aged↗