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At least 955 records · Page 53Linked to original sources
PubMed1989

Adenine nucleotide contents and ATPases activities in porcine deciduous dental pulp during the root formation, in fully formed root and during root resorption phases.

Nucleotide content and activity of certain enzymes were compared in pigs of various ages in order to study the energetic metabolism of deciduous dental pulps in the three phases of the cycle of tooth ontogeny, namely, root formation, fully formed root and root resorption phases. The frozen pulps were removed with the help of a screw vise and analysed for ATP, ADP and AMP contents and Ca2+ and Mg2+-ATPases activities. The highest ATP content in the first deciduous molar pulp was found when the tooth was still in an intrabony position. The calculated energy charge, although low for all groups, at this stage of development, indicated an activation of the consuming processes. In the root resorption phase, lowest ATP content and higher Ca2+ and Mg2+-ATPases activities were observed.

Adenine Nucleotides↗
PubMed2006

Visualisation of human dental pulp vasculature by immunohistochemical and immunofluorescent detection of CD34: a comparative study.

CD34 is considered a pan-endothelial cell marker for paraffin-embedded sections. In this study, both immunohistochemistry and immunofluorescence were applied in human dental pulp specimens of moderate thickness (10 microm) in order to observe the vasculature of this tissue using CD34. Both techniques revealed a homogenous staining pattern with capillaries and larger vessels showing complete and strong membrane staining reflecting the high capacity of the pulp for regeneration and response to different stimuli. A novel approach in the identification of the pulpal vasculature by Cy5-conjugated anti-CD34 is introduced in this study. By this technique the dense capillary plexus of the sub-odontoblastic region, which is responsible for the reaction of the tissue to any physical or chemical stimuli or pathological condition, can be clearly identified, while immunohistochemistry did not reveal such a detailed staining pattern.

Adolescent↗
PubMed2001

Healing process of dog dental pulp after pulpotomy and pulp covering with mineral trioxide aggregate or Portland cement.

Considering several reports about the similarity between the chemical compositions of the mineral trioxide aggregate (MTA) and Portland cement (PC), the subject of this investigation was to analyze the behavior of dog dental pulp after pulpotomy and direct pulp protection with these materials. After pulpotomy, the pulp stumps of 26 roots of dog teeth were protected with MTA or PC. Sixty days after treatment, the animal was sacrificed and the specimens removed and prepared for histomorphological analysis. There was a complete tubular hard tissue bridge in almost all specimens. In conclusion, MTA and PC show similar comparative results when used in direct pulp protection after pulpotomy.

Aluminum Compounds↗
PubMed1992

Activation of cathepsin B involved in enkephalin production by bradykinin and its cleavage products in cultured fibroblasts of the rat dental pulp.

Mechanisms of cathepsin B activation involved in methionine-enkephalin (ME) production induced by bradykinin (BK), des-Arg9-BK or L-arginine (L-Arg) were studied using cultured fibroblasts of the rat dental pulp, especially from a viewpoint of intracellular signal transduction. BK, des-Arg9-BK, L-Arg or cysteine enhanced the release of ME-like peptides from the cells, and the release of ME-like peptides induced by des-Arg9-BK was inhibited by des-Arg9-[Leu8]-BK (BK B1-receptor antagonist) and E-64 (a specific inhibitor of cysteine proteinases). The activation of cathepsin B by BK or des-Arg9-BK was inhibited by des-Arg9-[Leu8]-BK or islet-activating protein (IAP), and the activation of cathepsin B by L-Arg was inhibited by Leu-Arg (kyotorphin-receptor antagonist) or Botulinum C3-enzyme. The activation of cathepsin B by those stimulants was dependent on calcium ion. These results suggest that the ME production by BK or des-Arg9-BK may be mediated by Ca(2+)-dependent cathepsin B activation through B1-receptors and IAP-sensitive G-proteins, whereas the production by L-Arg may be mediated by Ca(2+)-dependent cathepsin B activation through kyotorphin-receptor and Botulinum C3-enzyme-sensitive G-proteins. On the other hand, the activation of cathepsin B was inhibited by neomycin B (phospholipase C inhibitor) and various serine/threonine kinase inhibitors. These results indicate that phospholipase C and serine/threonine kinases are involved in the activation of cathepsin B by BK, des-Arg9-BK or L-Arg. Genistein inhibited the activation of cathepsin B by des-Arg9-BK or L-Arg in a different fashion, suggesting that tyrosine kinase(s) is also involved in the activation. Cathepsin B activation by BK or L-Arg but not des-Arg9-BK was inhibited by L-NMMA (inhibitor of NO synthesis), and the activation by L-Arg was enhanced by beta-glycerophosphate (beta-GP: inhibitor of phosphatases), while the activation by BK or des-Arg9-BK was inhibited by beta-GP. These results suggest that BK-induced cathepsin B activation in the fibroblasts may be due to a combined effect of des-Arg9-BK and L-Arg.

Animals↗
PubMed1996

Nerve degeneration within the dental pulp after segmental osteotomies in the baboon (Papio ursinus).

Following dentofacial surgical procedures, teeth in segments often do not sense thermal or electric stimuli. This study was undertaken to assess changes in the neural component of the dental pulp after posterior maxillary and mandibular segmental osteotomies, with or without interpositional autogenous bone grafting, in 26 Chacma baboons. Innervation was assessed histologically immediately after operation, and at 3, 6, 12 and 18 months. Statistically significant differences were present between the experimental and control groups. Even after 18 months no nerves were present in any of the mandibular teeth. In maxillary teeth, 50 per cent had demonstrable nerves in the graft group and 40 per cent in the no graft group. As nerve degeneration was present in the experimental teeth, patients should be warned of possible change in tooth sensibility, following these operations. Careful post-operative follow up for long periods in humans following dentofacial surgical procedures is thus essential.

Animals↗
PubMed1995

Cytoskeleton of the mesenchymal cells of the rat dental papilla and dental pulp.

This study describes the immunolocalization of actin, cytokeratins and vimentin during differentiation of the dental papilla in the rat. Incisors and first molars were sectioned from mandibles of Wistar rats from embryonic day (E)-14 to (E)-21 and weeks 1, 2, 3, 12 and 104 after birth, fixed in 90% alcohol, decalcified in EDTA, infiltrated with 5% sucrose, frozen in dry ice, and cryosectioned at 10 microns. The sections were immunolabelled using indirect immunofluorescence with a panel of monoclonal antibodies and FITC-phalloidin for F-actin localization. F-actin was present in follicular mesenchyme and odontoblast processes. Vimentin labelled dental papilla fibroblasts, differentiating, functional (secretory) and aged odontoblasts. Vimentin was uniformly localized in the cytoplasm of pre-odontoblasts but was redistributed to the apical pole of these cells during polarization. Of the cytokeratins, only cytokeratin 19 was found in differentiating odontoblasts. It was not present in dental papilla fibroblasts, functional or aged odontoblasts. These results suggest that actin and the redistribution of vimentin may be involved in odontoblast differentiation and odontoblast process formation/support and that these events may be preceded by the expression of cytokeratin 19.

Actins↗