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At least 955 records · Page 53Linked to original sources
PubMed1994

[Effect of WS-frequence spectrum physiatrics apparatus on survival of the random flap in rats].

In this experimental study we investigated the effect of WS-frequence spectrum physiatrics apparatus on the survival of the random flap in the back of rats. The flap was radiated vertically with the WS-apparatus for 30 minutes, twice daily for 8 days. It was found that on the ninth postoperative day, the survival areas of the radiated flaps was increased significantly (P < 0.001). The isotope (99mTc) clearance, the diaphanousflap sample and SOD content were examined. The results showed that WS-apparatus can reduce the production of oxygen free radicle in the flap, increase the tolerance for ischemia and blood supply of the flap with the proliferation and vasodilation of capillary vessels.

Animals↗
PubMed1994

Automated blot hybridization and washing apparatus.

An apparatus that automates the hybridization and washing of blots is described. With this apparatus, blots are hybridized and washed inside of open-ended tubes that rotate within stationary bottles. The blots are washed in the bottles by the automatic addition and removal of solutions eliminating contact between researchers and contaminated solutions. The apparatus has been routinely used for blot hybridizations of soybean genomic DNA using single-copy DNA clones as probes. The quality of the autoradiograms from these hybridizations was similar to that obtained with a rotisserie-style hybridization system currently used in many labs.

Nucleic Acid Hybridization↗
PubMedDate not supplied

[Prevention of cross contamination, patient to anesthesia apparatus to patient, using filters].

Concern for cross infections from patient to patient via apparatus is particularly relevant today. There are several ways to prevent patient contamination through anesthetic devices. Although there is no clinical evidence for using one alternative over another and each hospital establishes its own hygienic protocols, we have introduced the systematic use of filters with patients undergoing general anesthesia. We describe the features of filters available on the market and our protocol for their use. The efficacy of a filter depends on whether bacteria or viruses are to be controlled. Filters can be classified into three groups or generations based on mesh quality: 1) heat and humidity exchangers (HHE), with large mesh screens that allow water to pass through; 2) bacterial filters (FHHE), with finer mesh that is permeable to droplets of water and 3) folded membrane filters (FHHE) that are hydrophobic, with very fine mesh that stops water. We describe three basic physical tests (passage of water, passage of smoke and increase of resistance when applied to the patient) for filters to be classified. The ideal filter is hydrophobic and does not increase circuit resistance over the amount specified. Four principles are emphasized in the protocol: 1) the filter forms a part of the patient, not the apparatus; 2) proper placement of the filter is between the patient and the circuit's "Y" piece; 3) the main purpose of the filter is to prevent contamination of the apparatus, and 4) if a hydrophobic filter is used with each patient, the use of a disposable respiratory circuit is not called for.

Adult↗
PubMed1993

[Practical methods used in the system of controlling of x-ray apparatus].

An x-ray apparatus like any other device becomes worn out. In order to avoid x-ray examinations of patients performed using a worn out apparatus a periodical control of such appliances should be carried out. This work presents a methodology based on simple quality tests for a standard x-ray apparatus. These tests measure parameters of x-ray tubes and beams of radiation. A positive feature of these tests is that they replace very expensive specialistic measuring equipment.

Equipment Failure↗
PubMed1995

[Effects of preserving mitral apparatus on left ventricular systolic function and geometry in mitral valve replacement].

We investigated the left ventricular (LV) systolic function and geometry in mitral valve replacement (MVR) with and without preservation of mitral apparatus. Five patients had conventional MVR without mitral annulus papillary muscle continuity (group C) and five patients had MVR with preservation of the continuity (group P). LV cineangiograms were assessed before and one month after surgery. LV volume was calculated by the method of Kennedy. Long axis dimension is defined as the distance between the apex and the center of mitral orifice in right anterior oblique projection. Short axis dimension is defined as the cross-sectional dimension at the middle point of the long axis. Paired Student's t tests were employed to compare the preoperative and postoperative data. LV ejection fraction decreased significantly from 69.4 +/- 9.5 to 51.8 +/- 10.5% in group C (p < 0.05) and did not change significantly in group P (from 51.6 +/- 19.9 to 56.8 +/- 12.9%). Fractional shortening of the long axis increased significantly from 15.0 +/- 11.8 to 21.2 +/- 14.0% in group P (p < 0.02) and did not change significantly in group C (from 16.5 +/- 8.6 to 13.1 +/- 3.3%). Ratio of LV end-systolic long axis to short axis dimension increased significantly from 1.76 +/- 0.31 to 2.12 +/- 0.21 in group C (p < 0.05) and did not change significantly in group P (from 1.90 +/- 0.22 to 1.95 +/- 0.22). We conclude that the ratio of systolic long to short axis increases, i.e., systolic LV shape changes to become longer and slender, and ejection fraction decreases after surgery in MVR without preservation of mitral apparatus. Conversely, preserving the mitral apparatus in MVR results in no significant change in systolic LV function and geometry, because of preserving fractional shortening of long axis.

Aged↗
PubMed1996

[Clinical study of imposed work of breathing caused by the breathing apparatus].

OBJECTIVE: The imposed work of breathing caused by the breathing apparatus (endotracheal tube, breathing circuit tubing, and ventilator's valves) may increase patient's work of breathing. To evaluate the imposed work may be useful to determine weaning. METHODS: Work of breathing was observed by Bicore CP-100 breathing monitor at different levels pressure support (PS), T piece and after extubation, and the PS level to compensate imposed work of breathing was computed. RESULTS: When PS level decreased, patient's work of breathing increased significantly. At PS O kPa, the patient's work of breathing increased 57%, which was caused by imposed work of apparatus. Because of the resistance of endotracheal tube, the patient's work of breathing increased 41% at T piece. The PS level that compensated for the imposed work was 0.7 kPa. CONCLUSIONS: When PS level was too low or T piece was applied, the imposed work of breathing caused by apparatus could markedly increase patient's work of breathing.

Adult↗
PubMed1998

[A comparative analysis of the use of Uzor and Optodan laser apparatus for the prevention and combined treatment of pulpitis and periodontitis].

Clinical research 242 patients for comparison purposes of efficiently of employment of semiconductor laser apparatus "Uzor" and "Optodan" as physiotherapeutic of the factor for preventive maintenance and treatment of complications, arising the ambassador endodontic of treatment is spent. Results of application of semi-conductor laser apparatus are resulted at the various forms pulpitis and periodontitis. Significant reduction of a pain directly after a procedure LSL is marked. The highest efficiency is established by use of the apparatus "Optodan", connected with distinction configurations of a impulse.

Adolescent↗
PubMed1998

[Plastic remodeling of the synaptic apparatus of skeletal muscles under physiological and pathological conditions].

The synaptic apparatus of skeletal muscles undergoes continuous remodelling, by adapting to the functioning level of the peripheral neuromuscular apparatus under normal conditions, compensating and restoring its impaired function in pathology. This reorganization has specific features depending on the factors that influence it, such as increased function, decreased or no function, neuronal and nerve damage, synaptic toxin intoxication, autoantibody action, disturbances of trophic influences, etc. Damage to any link of the neuromuscular apparatus due to the above factors is likely to be an essential condition for remodelling.

Animals↗
PubMed2002

Inheritance of the endoplasmic reticulum and Golgi apparatus.

Yeast and mammalian cells use a variety of different mechanisms to ensure that the endoplasmic reticulum and Golgi apparatus are inherited by both daughter cells on cell division. In yeast, endoplasmic reticulum inheritance involves both active microtubule and passive actin-based mechanisms, while the Golgi is transported into the forming daughter cell by an active actin-based mechanism. Animal cells actively partition the endoplasmic reticulum and Golgi apparatus, but association with the mitotic spindle-rather than the actin cytoskeleton-appears to be the mechanism

Actins↗
PubMed1998

Localization of Tctex-1, a cytoplasmic dynein light chain, to the Golgi apparatus and evidence for dynein complex heterogeneity.

To date, much attention has been focused on the heavy and intermediate chains of the multisubunit cytoplasmic dynein complex; however, little is known about the localization or function of dynein light chains. In this study, we find that Tctex-1, a light chain of cytoplasmic dynein, localizes predominantly to the Golgi apparatus in interphase fibroblasts. Immunofluorescent staining reveals striking juxtanuclear staining characteristic of the Golgi apparatus as well as nuclear envelope and punctate cytoplasmic staining that often decorates microtubules. Tctex-1 colocalization with Golgi compartment markers, its distribution upon treatment with various pharmacological agents, and the cofractionation of Tctex-1-associated membranes with Golgi membranes are all consistent with a Golgi localization. The distribution of Tctex-1 in interphase cells only partially overlaps with the dynein intermediate chain and p150(Glued) upon immunofluorescence, but most of Tctex-1 is redistributed onto mitotic spindles along with other dynein/dynactin subunits. Using sequential immunoprecipitations, we demonstrate that there is a subset of Tctex-1 not associated with the intermediate chain at steady state; the converse also appears to be true. Distinct populations of dynein complexes are likely to exist, and such diversity may occur in part at the level of their light chain compositions.

Amino Acid Sequence↗
PubMed2000

Interaction of casein kinase 1 delta (CK1delta) with post-Golgi structures, microtubules and the spindle apparatus.

Members of the casein kinase 1 family of serine/threonine kinases are highly conserved from yeast to mammals and seem to play an important role in vesicular trafficking, DNA repair, cell cycle progression and cytokinesis. We here report that in interphase cells of various mammalian species casein kinase 1 delta (CK1delta) specifically interacts with the trans Golgi network and cytoplasmic, granular particles that associate with microtubules. Furthermore, at mitosis CK1delta is recruited to the spindle apparatus and the centrosomes in cells, which have been exposed to DNA-damaging agents like etoposide or gammairradiation. In addition, determination of the affinity of CK1delta to different tubulin isoforms in immunoprecipitation-Western analysis revealed a dramatically enhanced complex formation between CK1delta and tubulins from mitotic extracts after introducing DNA damage. The high affinity of CK1delta to the spindle apparatus in DNA-damaged cells and its ability to phosphorylate several microtubule-associated proteins points to a regulatory role of CK1delta at mitosis.

3T3 Cells↗
PubMed1990

Evaluation of the lacrimal apparatus with digital subtraction macrodacryocystography.

Patients who suffer from epiphora can benefit from reconstructive surgery in many cases of obstructive and nonobstructive lesions of the lacrimal apparatus. We describe our technique of digital subtraction macrodacryocystography (DSM) and discuss its efficacy in the evaluation of various abnormalities involving the lacrimal drainage pathway. A variety of pathologic conditions of the lacrimal apparatus are portrayed. DSM is an accurate, easy to perform, but relatively unrecognized method of anatomic localization of obstructive lesions within the lacrimal drainage system.

Dacryocystitis↗
PubMed1985

CT of the inferomedial orbit and the lacrimal drainage apparatus: normal and pathologic anatomy.

The normal and pathologic CT anatomy of the lacrimal drainage apparatus was examined during study of the orbits, nasal cavity, and paranasal sinuses in 100 patients with a variety of clinical complaints related to the inferomedial orbit. The bony lacrimal fossa, the nasolacrimal canal, and the fluid- or air-filled lacrimal sac and nasolacrimal duct were readily recognized in all cases. The lacrimal fossa and sac are found at the inferomedial orbit and are preseptal structures. Cystic expansion of the lacrimal sac (dacryocystitis) may mimic orbital abscess clinically; however, the radiographic recognition of a cystic, peripherally enhancing mass centered at the lacrimal fossa should exclude postseptal abscess and permit more conservative therapy. Obstruction or invasion of the drainage apparatus by tumor, infection, or posttraumatic scarring is readily depicted by CT. Sagittal/coronal images reformatted from thin transverse axial sections are often useful in defining the origin of an inferomedial orbital mass and its relation to the lacrimal sac when clinical studies and axial CT findings are equivocal.

Adult↗
PubMed1979

[Ultrastructural cytochemistry of the mouse juxtaglomerular apparatus (author's transl)].

The ultrastructural cytochemistry of the mouse juxtaglomerular apparatus has been studied. The specific granules of the juxtaglomerular cells were found to be argentaphobic when ultrathin sections of araldite-embedded renal cortex were stained according to the periodic acid-thio-carbohydrazide-silver proteinate technique of Thiery. This technique revealed an abundant glycogen of type beta in the specific granules and in the cytosol of these cells. The rim of specific granules was positive when ultrathin sections of glutaraldehyde-fixed, glycol methacrylate-embedded kidneys were stained with phosphotungstic-hydrochloric acids at a low pH. A reaction was also shown by the cell coat, lysosomes, autophagolysosomes, residual bodies and part of the Golgi complex. These results indicate that the specific granules of the mouse juxtaglomerular apparatus contains glycoproteins that are glycosylated in the Golgi complex.

Animals↗
PubMed1997

Effects of intracellular pH on the mitotic apparatus and mitotic stage in the sand dollar egg.

The effect of change in intracellular pH (pHi) on mitosis was investigated in the sand dollar egg. The pHi in the fertilized egg of Scaphechinus mirabilis and Clypeaster japonicus, which was 7.34 and 7.31, respectively, changed by means of treating the egg at nuclear envelope breakdown with sea water containing acetate and/or ammonia at various values of pH. The mitotic apparatus at pHi 6.70 became larger than that of normal fertilized eggs; that is, the mitotic spindle had the maximal size, especially in length at pHi 6.70. The spindle length linearly decreased when pHi increased from 6.70 to 7.84. By polarization microscopy, the increase in birefringence retardation was detected at slightly acidic pHi, suggesting that the increase in size of the spindle is caused by the increase in the amount of microtubules in the spindle. At pHi 6.30, the organization of the mitotic apparatus was inhibited. Furthermore, slightly acidic pHi caused cleavage retardation or inhibition. By counting the number of the eggs at various mitotic stages with time after treating them with the media, it is found that metaphase was persistent and most of the S. mirabilis eggs were arrested at metaphase under the condition of pHi 6.70. It is concluded that at slightly acidic pH, the microtubules in the spindle are stabilized and more microtubules assembled than those in the normal eggs.

Acids↗
PubMed1998

Detection of P-glycoprotein in the Golgi apparatus of drug-untreated human melanoma cells.

The intracellular location of the MDR1 gene product, known as P-glycoprotein (P-gp), has been detected by flow cytometry in 3 stabilized human melanoma cell lines which had never undergone cytotoxic drug treatment and did not express P-gp on the plasma membrane. In addition, MDR1 mRNA expression was revealed by RT-PCR in the same cell lines. Immunofluorescence microscopy, performed by using the same 2 monoclonal antibodies (MM4.17 and MRK-16) as employed in the flow-cytometric analysis, revealed the presence of P-gp intracytoplasmically, in a well-defined perinuclear region. Double immunofluorescence labelling and immunoelectron microscopy strongly suggested the location of the transporter molecule in the Golgi apparatus. The same observations have been obtained on a primary culture from a metastasis of human melanoma. Analysis of the expression of another membrane transport protein, the multidrug-resistance-related protein (MRP1), showed that it was present in the cytoplasm of all the melanoma cell lines examined. MRP1 also showed Golgi-like localization. The study by laser scanning confocal microscopy on the intracellular localization of the anti-tumoral agent doxorubicin (DOX) during the drug-uptake and -efflux phases, indicated the Golgi apparatus as a preferential accumulation site for the anthracyclinic antibiotic. P-gp function modulators (verapamil and cyclosporin A) were able to modify DOX intracytoplasmic distribution and to increase drug intracellular concentration and cytotoxic effect in melanoma cells. On the contrary, MRP1 modulators (probenecid and genistein) did not significantly influence either DOX efflux and distribution or the sensitivity of melanoma cells to the cytotoxic drug.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1999

Isolation of plasma membranes and Golgi apparatus from a single chicken liver homogenate.

Chicken liver plasma membranes, minimally contaminated with Golgi apparatus-derived vesicles, were prepared from a low-speed (400 g) pellet by means of flotation in isotonic Percoll solution, followed by a hypotonic wash and flotation in a discontinuous sucrose gradient. Based on the analysis of suitable marker enzymes, alkaline phosphatase and alkaline phosphodiesterase, two plasma membrane fractions were isolated with enrichments, depending on the equilibrium density and marker of 28-97 and with a total yield of 4-5%. Golgi apparatus fractions were prepared by flotation of microsomes, obtained from the same homogenate as the low-speed pellet, in a discontinuous sucrose gradient. The trans-Golgi marker galactosyltransferase was 27-fold enriched in a fraction of intermediate density (d=1.077-1.116 g/ml). Approximately 12% of galactosyltransferase was recovered in the membranes equilibrating d=1.031-1.148 g/ml. Contamination with plasma membrane fragments was low in the light (d=1.031-1.077 g/ml) and intermediate density Golgi vesicles. The isolation of purified plasma membranes and Golgi vesicles from one liver homogenate will enable future studies on receptor cycling between these cell organelles.

Alkaline Phosphatase↗