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PubMed2003

Proximal tibial fracture stability with intramedullary nail fixation using oblique interlocking screws.

OBJECTIVES: The purpose of this study was to evaluate the mechanical stability of oblique interlocking screws in supplementing intramedullary nail fixation of high proximal tibial fractures. DESIGN: In vitro experimental testing. SETTING Orthopaedic biomechanics laboratory, Sunnybrook and Women's College Health Sciences Center. PARTICIPANTS: Ten paired fresh-frozen human cadaver tibiae. INTERVENTION: One tibia of each pair was randomized to be instrumented with an intramedullary nail (M/DN; Zimmer, Warsaw, Indiana), while the other was stabilized with a 13-hole stainless steel lateral tibial head plate (Synthes AO/ASIF). Specimens were tested in varus-valgus (v/v), flexion-extension (f/e) and torsion, before and after a 2-cm gap osteotomy was performed in the proximal segment. Testing of the nailed tibiae was performed with and without oblique proximal screws. Bone density was physically determined by removing a core of trabecular bone from the distal end of each tibia following testing. MAIN OUTCOME MEASUREMENT: Biomechanical construct stability. RESULTS: The addition of the proximally placed oblique screws increased the stability of the nail construct in v/v by 50% (6.8 mm, P < 0.05), in f/e by 47% (7.2 mm, P < 0.05), and in torsion by 18% (3.0 degrees, P < 0.05). There was no significant difference observed between the stability of the intramedullary nail construct with oblique screws and the plated construct. Trabecular bone density had a significant effect in reducing stability (P < 0.05) in nail and plate fixation. CONCLUSION: The addition of oblique interlocking screws significantly improves the stability of a nailed proximal tibia fracture and provides comparable stability to a plate osteosynthesis.

Aged↗
PubMed1999

Prophylactic ankle stabilizers affect ankle joint kinematics during drop landings.

PURPOSE: Ankle joint dorsiflexion contributes to energy absorption during landing, but wearing ankle stabilizers is known to restrict passive measures of dorsiflexion. This study compared the effects of various ankle stabilizers on ankle joint kinematics during soft and stiff landings. METHODS: Subjects (N = 14) performed two-legged landings off a 0.59-m platform. Kinematics of the right ankle were calculated from a sagittal plane video recording (120 Hz). Five soft and five stiff landings were performed in five ankle stabilizer conditions (no stabilizer, taping, Swede-O, AirCast, and Active Ankle), a total of 50 trials per subject. Style and stabilizer conditions were randomized across subjects. Each subject's five-trial mean value of selected kinematic variables for each landing style/stabilizer condition was entered into a two-way repeated MANOVA (alpha = 0.05). RESULTS: Differences between soft and stiff landing conditions were similar to those reported in the literature. Compared with the No stabilizer condition, most stabilizer conditions significantly reduced ankle dorsiflexion ROM and angular velocity during landing. CONCLUSIONS: The results indicate that some ankle stabilizers adversely affect ankle joint kinematics during landing.

Adult↗
PubMed1994

Biomechanical analysis of cervical stabilization systems. An assessment of transpedicular screw fixation in the cervical spine.

STUDY DESIGN: The biomechanical stability of seven cervical reconstruction methods including the transpedicular screw fixation was evaluated under four instability patterns. These four modalities, based on the range and grade of instability, allowed a reproducible biomechanical assessment to establish the in vitro role of internal fixation in the cervical spine. OBJECTIVES: This study biomechanically investigated the stability of seven reconstruction methods in the cervical spine as influenced by four instability patterns and assessed whether three-column fixation for the cervical spine using transpedicular screw fixation would provide increased stability over that of conventional cervical fixation systems. METHODS: A total of 24 calf cervical spine specimens were divided into four experimental groups. The spinal constructs including seven reconstruction techniques--the posterior AO titanium reconstruction plate, Bohlman's posterior triple-wiring, transpedicular screw fixation, anterior iliac bone graft, anterior AcroMed plate, anterior AO titanium locking plate, and combined fixation with the AO anterior plate and posterior triple-wiring--were tested under four loading modes. RESULTS: Anterior plating methods provided less stability than that of posterior constructs under axial, torsional, and flexural loading conditions. Exclusive posterior procedures provided increased stability compared with the intact spine in one level fixation, however, did not sustain the torsional stability when the anterior and middle column was eliminated in two-level fixation. The stabilizing capabilities of both the combined fixation and transpedicular screw fixation were clearly demonstrated in all loading modes, however, those of the latter were superior in multilevel fixation. CONCLUSION: Front and back approaches, employing the anterior plate and posterior triple-wiring, and transpedicular screw fixation demonstrated clear biomechanical advantages when the extent of instability increased to three-column or multilevel. Three-column fixation for the cervical spine using transpedicular screw fixation offers increased stability over that of conventional cervical fixation systems, particularly in multiple level constructs.

Animals↗
PubMed2002

Lumbosacral stability of consolidated anteroposterior fusion after instrumentation removal determined by roentgen stereophotogrammetric analysis and direct surgical exploration.

STUDY DESIGN: The intervertebral stability of bony consolidated anteroposterior lumbosacral spondylodesis is evaluated by roentgen stereophotogrammetric analysis and direct surgical exploration before and after removal of the internal fixator. OBJECTIVES: To determine the remaining in vivo stability of spinal arthrodesis solely retained by a bony integrated carbon fiber cage. SUMMARY OF BACKGROUND DATA: Roentgen stereophotogrammetric analysis studies on posterolateral lumbar fusions demonstrate primary spinal stability after additional dorsal instrumentation, which is retained during bony fusion healing. Animal models show a persistent stabilizing effect of the fixator despite the presence of bony fusion. Although direct surgical inspection is the most reliable method to evaluate fused vertebrae, roentgen stereophotogrammetric analysis has also proven to be a highly accurate method to evaluate spinal stability. METHODS: In 10 patients lumbosacral fusion was performed using carbon interbody implants and an internal fixator. Ten months after initial surgery (range 7-15 months) the internal fixation was removed to reduce local soft tissue impingement as soon as bony fusion was achieved. Fusion site exploration in the course of instrumentation removal was performed by applying distraction, compression, and torque to the grafted area under fluoroscopic control. Any motion indicated a pseudarthrosis. Lumbosacral stability was evaluated by serial roentgen stereophotogrammetric analysis after fusion and after instrumentation removal. RESULTS: During instrumentation removal the mechanical stress test under fluoroscopic control did not indicate pseudarthrosis. After instrumentation removal, roentgen stereophotogrammetric analysis measurements revealed a nonsignificant increase in lumbosacral micromotions within the fused segment with 0.14, 0.31, and 0.44 mm in the transverse, vertical, and sagittal axes, respectively. CONCLUSIONS: The internal fixator could be removed without endangering the stability of the fusion. Direct surgical exploration confirmed the adequacy of roentgen stereophotogrammetric analysis as a reliable in vivo method to evaluate lumbosacral stability after anteroposterior fusion.

Adult↗
PubMed2003

Dynamic stabilization of the lumbar spine and its effects on adjacent segments: an in vitro experiment.

In recent years, nonfusion stabilization of the lumbar spine has gained more and more popularity. These nonfusion systems intend to maintain or restore the intersegmental motions to magnitudes of the intact spine and have no negative effects on the segments adjacent to the stabilized one. This study investigated the DYNESYS, a dynamic nonfusion system, which is designed to stabilize the bridged segments while maintaining the disc and the facet joints. To determine the magnitude of stabilization and the effect of the stabilization on the adjacent segment, six lumbar cadaver spines were fixed in a spine tester and loaded with pure moments in the three main motion planes. For each spine, four different stages were tested: intact, defect of the middle segment, fixation with the DYNESYS, and fixation with the internal fixator. Intersegmental motions were measured at all levels. For the bridged segment, the DYNESYS stabilized the spine and was more flexible than the internal fixator. This difference between the internal fixator and the DYNESYS was most pronounced in extension (P < 0.05), with the DYNESYS restoring the motion back to the level of the intact spine. The motion in the adjacent segments was not influenced by either stabilization method. Our results suggest that the DYNESYS provides substantial stability in case of degenerative spinal pathologies and can therefore be considered as an alternative method to fusion surgery in these indications while the motion segment is preserved.

Adult↗
PubMed2004

Effect of supplemental translaminar facet screw fixation on the stability of stand-alone anterior lumbar interbody fusion cages under physiologic compressive preloads.

STUDY DESIGN: A biomechanical study of lumbar threaded interbody cage construct under varying compressive preloads of similar magnitudes to those experienced in vivo during daily activities. OBJECTIVES: To test the hypothesis that supplemental translaminar facet screws would enhance the stability (ability to reduce segmental angular motion) of threaded interbody cages in flexion-extension during activities in which the spine is subjected to low compressive preloads, and therefore the stand-alone interbody cage construct is least stable. SUMMARY OF BACKGROUND DATA: Controversy exists over whether threaded anteriorly placed interbody cages can be routinely used as "stand-alone" devices or whether they require supplemental posterior stabilization to achieve successful fusion. Biomechanical studies suggest that under conditions of low preloads, the motion segment treated with stand-alone cages might be less stable, particularly in extension. METHODS.: Eight human lumbar spine specimens (from L1 to sacrum) were tested intact, after insertion of 2 threaded cylindrical cages (BAK) at L5-S1 and after supplemental translaminar facet screw fixation. They were subjected to flexion and extension moments under progressively increasing magnitude of externally applied compressive follower preload from 0 to 1200 N. The range of angular motion in flexion-extension at L5-S1 was analyzed to assess the effect of translaminar facet screws on the stability of the cage construct for different compressive preloads. RESULTS: In flexion, over 0 to 400 N preload, the supplemental translaminar facet screw fixation reduced the L5-S1 angular motion relative to intact by 71% to 74% as compared to 40% to 44% for the cages alone. This difference was statistically significant (P < 0.05). In extension at 0 N preload, the cages allowed more angular motion than the intact segment, whereas with translaminar facet screw fixation, the motion was reduced to the level of the intact segment. At 400 N preload, supplemental TLFS fixation significantly increased the stability of the cages, reducing the extension angular motion by 60% of intact (P = 0.04). Supplemental translaminar facet screw fixation did not significantly increase the stability provided by the cages in flexion or extension at the 1200 N preload magnitude. CONCLUSIONS: In vivo during activities of daily living, interbody cage constructs are subject to varying compressive preloads due to external loads generated by paraspinal musculature, and our results suggest that the stability created by the cage (reduction in segmental angular motion) is not constant. The cage construct is likely to be least stable in extension during activities that impart low compressive preloads to the lumbar spine. Supplemental translaminar facet screw fixation will enhance stability of the motion segment treated with threaded cages, particularly during conditions of low compressive preloads, the very condition in which the cage alone is least effective in providing stability.

Adult↗
PubMed2006

Effects of abdominal muscle coactivation on the externally preloaded trunk: variations in motor control and its effect on spine stability.

STUDY DESIGN: A repeated measures biomechanical analysis of the effects of abdominal bracing in preparation for a quick release of the loaded trunk. OBJECTIVES: To quantify the ability of individuals to abdominally brace the externally loaded trunk, and assess their success in achieving and enhancing appropriate spine stability. SUMMARY OF BACKGROUND DATA: Spine stability requires trunk muscle coactivation, which demands motor control skill that differs across people and situations. The quick release protocol may offer insight into the motor control scheme and subsequent effect on spine stability. METHODS: There were 10 individuals who sat, torso upright, in an apparatus designed to foster a neutral spine position. They were instructed to support a posteriorly directed load to the trunk in either their naturally chosen manner, or by activating the abdominal muscles to 10%, 20%, or 30% of maximum ability. The externally applied load was then quickly released, thereby unloading the participant. Muscle pre-activation patterns, spine stability, and kinematic measures of trunk stiffness were quantified. RESULTS: Participants were able to stabilize their spine effectively by supporting the load in a naturally selected manner. Conscious, voluntary overdriving of this natural pattern often resulted in unbalanced muscular activation schemes and corresponding decreases in stability levels. CONCLUSIONS: Individuals in an externally loaded state appear to select a natural muscular activation pattern appropriate to maintain spine stability sufficiently. Conscious adjustments in individual muscles around this natural level may actually decrease the stability margin of safety.

Abdominal Muscles↗
PubMed2006

Effect of lumbar interbody cage geometry on construct stability: a cadaveric study.

STUDY DESIGN: Biomechanical study to investigate three-dimensional motion behavior of cadaveric spines in various surgical simulations. OBJECTIVES: To determine the effect of cage geometry on the construct stability. SUMMARY OF BACKGROUND DATA: There is a wide variety of cage/spacer designs available for lumbar interbody fusion surgery. These range from circular, tapered, and rectangular with and without curvature. However, the effectiveness of cages with different designs and materials to stabilize a decompressed intervertebral space has not been fully studied. METHODS: Six fresh ligamentous lumbar spine specimens (L1-S2) were subjected to pure moments in the six loading directions. The resulting spatial orientations of the vertebrae were recorded using Optotrak Motion Measurement System. Measurements were made sequentially for intact, bilateral spacer placements across L4-L5 using a posterior approach, supplemented with pedicle screw-rod system fixation, and after the cyclic loading in flexion-extension mode. RESULTS: The stability tended to decrease after the bilateral cage placement as compared with the intact for all loading cases except flexion. In flexion, the angular displacement decreased to 80% of the intact. However, there was no significant statistical difference seen in stability between intact and after bilateral spacer placement. Following the addition of posterior fixation using pedicle screw-rod system, the stability significantly increased in all directions. Cyclic loading did not have any significant effect on the stability. CONCLUSIONS: Stand-alone cages restore motion to near-intact levels at best, and supplement instrumentation is essential for significantly increasing the stability of the decompressed segment. The effects of cage geometry and Young's modulus of the cage material do not seem to influence the stability, as compared with the other cagedesigns, especially after supplemental fixation with a posterior system.

Aged↗
PubMed1999

The control of foot placement during compensatory stepping reactions: does speed of response take precedence over stability?

Rapid, reflex-like stepping movements are a prevalent and functional compensatory reaction to destabilization, however, little is known about the underlying control. In this paper, a model is developed to examine how speed and stability demands affect control of foot placement during forward and backward compensatory stepping reactions. The concept of the velocity stability margin (VSM) is introduced to characterize the degree to which the horizontal velocity of the falling body approaches biomechanical limits on the capacity to decelerate the center of mass; analogous limits on center-of-mass displacement are quantified in terms of the displacement stability margin (DSM). The model is used to predict, for any initial step characteristics, the variation in DSM and VSM that would occur as a function of changes in timing of foot placement. The VSM was found to prevail over the DSM in establishing limits of stability. Model simulations demonstrated that there typically exists a minimum swing duration that maximizes speed of response while meeting minimum requirements for stability (VSM > or = 0), as well as a slower speed of response (longer swing duration) at which stability (VSM) is maximized. Experimental data from platform-perturbation tests in 20 healthy young (22-28) and older (65-81) adults were used, in conjunction with the model, to investigate whether speed or stability takes precedence during natural behavior. Control of single-step reactions appeared to favor stability; although the model predicted that a minimally stable step (VSM = 0) could be attained by swing durations as short as 30 ms, the observed swing durations were, on average, 135 ms longer than this, and the average VSM was nearly as large (80%) as the optimally stable value predicted by the model. Control of the initial step of multiple-step reactions was distinctly different. The average swing duration was only 55 ms greater than the minimally stable value and the average VSM was 81% smaller than in the single-step reactions. This reduction in VSM is consistent with a need to execute additional steps and appears to support the validity of the model. This model may help to provide insight into the biomechanical factors that govern the neural control of compensatory stepping reactions.

Adult↗
PubMed2004

Analysis of the stability of multimeric proteins by effective DeltaG and effective m-values.

Analyzing the stability of a multimeric protein is challenging because of the intrinsic difficulty in handling the mathematical model for the folded multimer-unfolded monomer equilibrium. To circumvent this problem, we introduce the concept of effective stability, DeltaGeff (= -RTlnKeff), where Keff is the equilibrium constant expressed in monomer units. Analysis of the denaturant effect on DeltaGeff gives new insight into the stability of multimeric proteins. When a multimeric protein is mostly folded, the dependence of effective stability on denaturant concentration (effective m-value) is simply the m-value of its monomeric unit. However, when the protein is mostly unfolded, its stability depends on denaturant concentration with the m-value of its multimeric form. We also find that the effective m-value at the Cm is a good approximation of the apparent m-value determined by fitting the equilibrium unfolding data from multimeric proteins with a two-state monomer model. Moreover, when the m-value of a monomeric unit is estimated from its size, the effective stability of a multimeric protein can be determined simply from Cm and this estimated m-value. These simple and intuitive approaches will allow a facile analysis of the stability of multimeric proteins. These analyses are also applicable for high-throughput analysis of protein stability on a proteomic scale.

Models, Chemical↗
PubMed2000

Interaction with magnesium and ADP stabilizes both components of nitrogenase from Klebsiella pneumoniae against urea denaturation.

The nitrogenase enzyme of Klebsiella pneumoniae consists of two separable proteins, each with multiple subunits and one or more oxygen sensitive metallocenters. The wild-type nitrogenase proteins are stable to electrophoresis in high concentrations of urea under anaerobic conditions. Addition of Mg+2 and ADP greatly increases the stability of the smaller Fe protein (from <4 to >6 M for full unfolding), an effect directly analogous to stabilization in p21ras induced by Mg+2 and GDP. Stabilization by Mg+2 is slight for the holo MoFe protein (from approximately 1.5 to approximately 2.4 M) but more dramatic for the apo protein form of the MoFe protein accumulated by certain Fe protein (nifH gene) mutants. The potent product inhibitor of nitrogenase function, MgADP, increases stability of the MoFe protein more than Mg+2 alone, to approximately 3.6 M, showing that nucleotides interact with the MoFe protein. Mutations of the nifM gene result in slower accumulation of less stable Fe protein, indicating that NifM is involved in correct folding of the Fe protein. Mutationally altered proteins are often difficult to purify for study because of their inherent instability, low expression level, or oxygen lability. Crude extracts of 11 different mutants of Fe protein (nifH gene) were examined by transverse urea gradient gels to rapidly screen for stabilizing interactions in the presence or absence of substrate or inhibitor analogs. Amino acid alterations D44N and R188C, at the interface of the dimer, in the vicinity of the nucleotide binding site(s), have significantly lower stability than the wild-type enzyme in the absence of Mg+2 but comparable stability in its presence, showing the importance of Mg+2 in the subunit interactions. Mutations N163S and E266K, in which residues normally involved in hydrogen bonding far from the active site were altered, are more labile than the wild-type even with Mg+2 added. Seven other mutants, though nonfunctional, did not appear altered in stability compared to the wild-type.

Adenosine Diphosphate↗
PubMed1999

Lateral stability, sensorimotor function and falls in older people.

AIMS: To design simple tests of lateral stability for assessing balance in older people and to determine whether poor performances in these tests are associated with impaired vision, lower limb sensation, quadriceps strength, simple reaction time, and falling in this group. DESIGN: A cross-sectional and retrospective study. SETTING: Falls and Balance Laboratory, Prince of Wales Medical Research Institute. PARTICIPANTS: One hundred fifty-six community-dwelling men and women aged 63-90 years (mean age 76.5, SD = 5.1). OUTCOME MEASURES: The maximal lateral sway in a near-tandem stability test with eyes open and closed and the necessity of taking a protective step in the near-tandem stability test with eyes closed. RESULTS: All 156 subjects could complete the near-tandem stability test with eyes open, but only 99 subjects (63.5%) could undertake the test with eyes closed without taking a protective step. Subjects with a history of falls had increased lateral sway both with eyes open and eyes closed as well as poorer visual acuity, proprioception, and quadriceps strength. Fallers were also significantly more likely to take a protective step when undertaking the near-tandem stability test with eyes closed. Multiple regression analysis revealed that impaired lower limb proprioception, quadriceps strength, and reaction time were the best predictors of increased maximal sway in the near-tandem stability test with eyes open. Reduced proprioception and quadriceps strength, in addition to age, were also found to be the best determinants of the necessity of taking a protective step in the near-tandem stability test with eyes closed. CONCLUSIONS: The study findings identify simple new tests that are associated with falling in older people and elucidate the relative importance of specific physiological systems in the maintenance of lateral stability.

Accidental Falls↗
PubMed1995

Stability: an ICD detection criterion for discriminating atrial fibrillation from ventricular tachycardia.

INTRODUCTION: The purpose of this study was to review a new implantable cardioverter defibrillator (ICD) detection criterion, stability, to determine if it can effectively discriminate rapid rhythms of atrial fibrillation from ventricular tachycardia. Inappropriate shocks for rapid atrial fibrillation limit the acceptance of ICDs. The advent of an additional detection criterion, stability, has been postulated to be of value in discriminating rapid atrial fibrillation, which may not warrant treatment, from ventricular tachycardia, which obviously does warrant therapy deliver. METHODS AND RESULTS: Twenty-six patients were studied during 32 episodes of rapid atrial fibrillation and 24 episodes of monomorphic ventricular tachycardia below 220 beats/min. Each rhythm was repeatedly evaluated by the device at each of the seven stability values available (8, 16, 23, 31, 39, 47, and 55 msec) and then classified as stable or unstable. Upon completion of this acute study, 32 ICD patients had the stability feature activated and were followed for proper arrhythmia treatment by the device. Using stability windows from 8 to 47 msec, all atrial fibrillation rhythms were appropriately classified as unstable. Three of 6 were classified correctly for the 55-msec window. All ventricular tachycardia rhythms were appropriately classified as stable from all stability windows from 8 to 55 msec. Clinical follow-up confirmed appropriate therapy delivery when coupled with sustained rate duration (SRD). Thirty-two patients followed for 292 patient-months had no episodes of untreated ventricular tachycardia with 428 successfully classified as stable and treated. Only three episodes of suspected atrial fibrillation resulted in therapy delivery as the rhythm duration exceeded the SRD of 30 seconds. CONCLUSIONS: The CPI Ventak PRx ICD is highly reliable in appropriately classifying atrial fibrillation as unstable and monomorphic ventricular tachycardia as stable for most stability windows evaluation tachycardias below 220 beats/min. As a result, when testing of atrial fibrillation is not possible, we recommend the routing programming of this stability feature at the 31-msec window with an SRD of 30 seconds. The reliability of this device in discriminating atrial fibrillation from monomorphic ventricular tachycardia may have important clinical implications for other tiered therapy ICDs with this feature as well as for future ICDs in development.

Adult↗
PubMed1994

Stabilization of the tumor suppressor p53 during cellular transformation by simian virus 40: influence of viral and cellular factors and biological consequences.

To understand the process and biological significance of metabolic stabilization of p53 during simian virus 40 (SV40)-induced cellular transformation, we analyzed cellular and viral parameters involved in this process. We demonstrate that neither large T expression as such nor the cellular phenotype (normal versus transformed) markedly influence the stability of p53 complexed to large T in SV40 abortively infected BALB/c mouse fibroblasts. In contrast, metabolic stabilization of p53 is an active cellular event, specifically induced by SV40. The ability of SV40 to induce a cellular response leading to stabilization of p53 complexed to large T is independent from the cellular phenotype and greatly varies between different cells. However, metabolic stability was conferred only to p53 in complex with large T, whereas the free p53 in these cells remained metabolically unstable. Comparative analyses of cellular transformation in various cells differing in stability of p53 complexed to large T upon abortive infection with SV40 revealed a strong correlation between the ability of SV40 to induce metabolic stabilization and its transformation efficiency. Our data suggest that metabolic stabilization and the ensuing enhanced levels of p53 are important for initiation and/or maintenance of SV40 transformation.

3T3 Cells↗
PubMed1975

Surgical stabilization of traumatic flail chest.

Since 1970 we have stabilized the ribs to correct paradoxical movement of the chest wall in chest injuries, using an original technique, in order to avoid as far as possible the need for long-term chest wall stabilization by intermittent positive pressure respiration (IPPR). The technical details of surgical stabilization are described, and the different types of stainless steel struts are shown. Type I was originally used either as an intramedullary nail or as an external brace. Types II and III were designed for external fixation of the strut to the rib. Treatment of 29 patients with severe flail chest, classified into four groups is shown: group I was treated by IPPR, group II by IPPR plus surgical stabilization, group III by surgical stabilization only, and group IV by surgical stabilization after exploratory thoracotomy. The clinical results are discussed. We conclude that surgical stabilization of the paradoxial movement of the chest wall can avoid the use of the respirator or at least reduce the interval of IPPR to a short period during the initial recovery from trauma. Using type III struts, we have obtained stabilization of the flail chest in all cases even in patients with severe anterior paradoxical movement. The patients' tolerance of surgical stainless steel struts was good.

Humans↗
PubMed2005

Atomic force microscopy investigation of chemically stabilized pericardium tissue.

Native and chemically stabilized porcine pericardium tissue was imaged by the contact mode atomic force microscopy (AFM), in air. Chemically stabilized pericardium is used as a tissue-derived biomaterial in various fields of the reconstructive and replacement surgery. Collagen type I is the main component of the fibrous layer of the pericardium tissue. In this study, the surface topography of collagen fibrils in their native state in tissue and after chemical stabilization with different cross-linking reagents: glutaraldehyde (GA), dimethyl suberimidate (DMS) and tannic acid (TA) was investigated. It has been found that chemical stabilization causes considerable changes in the surface topography of collagen fibrils as well as in the spatial organization of the fibrils within the tissue. The observed changes in the D-spacing pattern of the collagen fibril correspond to the formation of intrafibrilar cross-links, whereas formation of interfibrilar cross-links is mainly responsible for the observed tangled spatial arrangement of fibrils and crimp structure of the tissue surface. The crimp structure was distinctly seen for the GA cross-linked tissue. Surface heterogeneity of the cross-linking process was observed for the DMS-stabilized tissue. SDS-PAGE electrophoresis was performed in order to evaluate the stabilization effect of the tissues treated with the cross-linking reagents. It has been found that stabilization with DMS, GA or TA enhances significantly the tissue resistance to SDS/NaCl extraction. The relation between the tissue stability and changes in the topography of the tissue surface was interpreted in terms of different nature of cross-links formed by DMS, GA and TA with collagen.

Animals↗
PubMed2002

Hypoxic but not anoxic stabilization of HIF-1alpha requires mitochondrial reactive oxygen species.

The molecular mechanisms by which cells detect hypoxia (1.5% O2), resulting in the stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) protein remain unclear. One model proposes that mitochondrial generation of reactive oxygen species is required to stabilize HIF-1alpha protein. Primary evidence for this model comes from the observation that cells treated with complex I inhibitors, such as rotenone, or cells that lack mitochondrial DNA (rho(0)-cells) fail to generate reactive oxygen species or stabilize HIF-1alpha protein in response to hypoxia. In the present study, we investigated the role of mitochondria in regulating HIF-1alpha protein stabilization under anoxia (0% O2). Wild-type A549 and HT1080 cells stabilized HIF-1alpha protein in response to hypoxia and anoxia. The rho(0)-A549 cells and rho(0)-HT1080 cells failed to accumulate HIF-1alpha protein in response to hypoxia. However, both rho(0)-A549 and rho(0)-HT1080 were able to stabilize HIF-1alpha protein levels in response to anoxia. Rotenone inhibited hypoxic, but not anoxic, stabilization of HIF-1alpha protein. These results indicate that a functional electron transport chain is required for hypoxic but not anoxic stabilization of HIF-1alpha protein.

Adenosine Triphosphate↗
PubMed2001

Mechanisms underlying stabilization of temporally summated muscle contractions in the lobster (Panulirus) pyloric system.

Muscles are the final effectors of behavior. The neural basis of behavior therefore cannot be completely understood without a description of the transfer function between neural output and muscle contraction. To this end, we have been studying muscle contraction in the well-investigated lobster pyloric system. We report here the mechanisms underlying stabilization of temporally summating contractions of the very slow dorsal dilator muscle in response to motor nerve stimulation with trains of rhythmic shock bursts at a physiological intraburst spike frequency (60 Hz), physiological cycle periods (0.5-2 s), and duty cycles from 0.1 to 0.8. For temporal summation to stabilize, the rise and relaxation amplitudes of the phasic contractions each burst induces must equalize as the rhythmic train continues. Stabilization could occur by changes in rise duration, rise slope, plateau duration, and/or relaxation slope. We demonstrate a generally applicable method for quantifying the relative contribution changes in these characteristics make to contraction stabilization. Our data show that all characteristics change as contractions stabilize, but their relative contribution differs depending on stimulation cycle period and duty cycle. The contribution of changes in rise duration did not depend on period or duty cycle for the 1-, 1.5-, and 2-s period regimes, contributing approximately 30% in all cases; but for the 0.5-s period regime, changes in rise duration increased from contributing 25% to contributing 50% as duty cycle increased from 0.1 to 0.8. At all cycle periods decreases in rise slope contributed little to stabilization at small duty cycles but increased to contributing approximately 80% at high duty cycles. The contribution of changes in plateau duration decreased in all cases as duty cycle increased; but this decrease was greater in long cycle period regimes. The contribution of changes in relaxation slope also decreased in all cases as duty cycle increased; but for this characteristic, the decrease was greatest in fast cycle period regimes, and in these regimes at high duty cycles these changes opposed contraction stabilization. Exponential fits to contraction relaxations showed that relaxation time constant increased with total contraction amplitude; this increase presumably underlies the decreased relaxation slope magnitude seen in high duty cycle, fast cycle period regimes. These data show that changes in no single contraction characteristic can account for contraction stabilization in this muscle and suggest that predicting muscle response in other systems in which slow muscles are driven by rapidly varying neuronal inputs may be similarly complex.

Animals↗