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At least 937 records · Page 52Linked to original sources
PubMed1994

Type III collagen is a major component of interodontoblastic fibers of the developing mouse molar root.

BACKGROUND: Recently, collagenous interodontoblastic fibers (IOF) were reported in some particular developmental stages and/or locations of the tooth. However, it remained unclear whether these fibers were identical to so-called von Korff fibers. METHODS: To clarify this issue, we examined the developing mouse molar by three-dimensional reconstruction of 8 confocal images within a 6 micron-thick section using laser scanning confocal microscopy, and confirmed our findings using immunoelectron microscopy. RESULTS: In the root pulp during circumpulpal dentin formation, the IOF stained weakly for type I collagen, but stained strongly for type III collagen by a double-staining technique. It could be clearly seen that many immunoreactive fibers ran spirally among the odontoblasts and entered the predentin. This distribution pattern of IOF was similar to that of the classical von Korff fibers. Furthermore, the existence of anti-type III reactive collagen fibrils between odontoblasts was confirmed, whereas IOF were not observed in the coronal pulp during circumpulpal dentin formation. CONCLUSIONS: This study presents for the first time, immunohistochemical observations which demonstrate the presence of IOF at least during root circumpulpal dentin formation and which reveal that type III collagen is a major component of IOF.

Animals↗
PubMed1996

Postnatal development and aging of the rat ventral root L5: electron microscopic and immunohistochemical studies.

The ventral root L5 of neonatal and adult rats has been used in many experimental studies on ganglionic C-fibers. Since the normal presence of such axons in L5 roots from animals of different ages is unknown, the results of these studies cannot be appropriately interpreted. In the present study we examine L5 ventral roots from developing and aging rats in this respect. Electron microscopic examination revealed that C-fibers occur in neonatal roots. The adult proportion has been established at day 30. Immunohistochemical analysis showed that thin ganglionic fibers with substance P/calcitonin gene-related peptide- or tyrosine hydroxylase-like immunoreactivity in the L5 root and the spinal pia mater seem to increase postnatally from low levels at birth. In roots from aged rats, myelinated fibers with a variety of aberrant features occur in normal numbers. The occurrence of unmyelinated axons is elevated. The increased presence of fibers with calcitonin gene-related peptide- or tyrosine hydroxylase-like immunoreactivity in aged roots indicates that the extra unmyelinated fibers may represent motor sprouts and sympathetic fibers, respectively. We conclude that the rat ventral root L5 contains a variable number of putative sensory and sympathetic axons at all ages.

Adrenergic Fibers↗
PubMed2001

Antisense expression of an Arabidopsis ran binding protein renders transgenic roots hypersensitive to auxin and alters auxin-induced root growth and development by arresting mitotic progress.

We cloned a cDNA encoding an Arabidopsis Ran binding protein, AtRanBP1c, and generated transgenic Arabidopsis expressing the antisense strand of the AtRanBP1c gene to understand the in vivo functions of the Ran/RanBP signal pathway. The transgenic plants showed enhanced primary root growth but suppressed growth of lateral roots. Auxin significantly increased lateral root initiation and inhibited primary root growth in the transformants at 10 pM, several orders of magnitude lower than required to induce these responses in wild-type roots. This induction was followed by a blockage of mitosis in both newly emerged lateral roots and in the primary root, ultimately resulting in the selective death of cells in the tips of both lateral and primary roots. Given the established role of Ran binding proteins in the transport of proteins into the nucleus, these findings are consistent with a model in which AtRanBP1c plays a key role in the nuclear delivery of proteins that suppress auxin action and that regulate mitotic progress in root tips.

Amino Acid Sequence↗
PubMed2004

The enl mutants enhance the lrx1 root hair mutant phenotype of Arabidopsis thaliana.

The development of root hairs serves as an excellent model to study cell growth using both cytological and genetic approaches. In the past, we have characterized LRX1, an extracellular protein of Arabidopsis consisting of an LRR-domain and a structural extensin domain. LRX1 is specifically expressed in root hairs and lrx1 mutants show severe deficiencies in root hair development. In this work, we describe the characterization of enl (enhancer of lrx1) mutants that were isolated in a visual screen of an ethylmethanesulfonate -mutagenized lrx1 line for plants exhibiting an enhanced lrx1 phenotype. Four recessive enl mutants were analyzed, three of which define new genetic loci involved in root hair development. The mutations at the enl loci and lrx1 result in additive phenotypes in enl/lrx1 double mutants. One enl mutant is affected in the ACTIN2 gene and encodes a protein with a 22 amino acid deletion at the C-terminus. The comparison of molecular and phenotypic data of different actin2 alleles suggests that the truncated ACTIN2 protein is still partially functional.

Actins↗
PubMed1989

Acetylcholinesterase in the development of chick dorsal root ganglia.

Acetylcholinesterase is expressed in chick dorsal root ganglia neurons very early in development. Since the physiological role of the enzyme in these cells is still obscure, it appeared of interest to investigate its modifications in the course of development. The specific activity of acetylcholinesterase in chick dorsal root ganglia increases, during in ovo development, from day E5 to day E13; after day E13 there is a decrease. Conversely, when acetylcholinesterase activity was expressed on a per ganglion basis, a continuous increase in the level of the enzyme until day E20 was observed. Acetylcholinesterase is a polymorphic enzyme and its molecular forms have different cellular localizations. Two globular forms, a tetramer (G4) and a dimer (G2), are present in the ganglia, as in chick brain. G4 is the major form at day E5, where it represents about 85% of the activity. This form shows a progressive decrease since day E8, and at day E20 exhibits activity levels similar to those of G2. It is known that acetylcholinesterase-producing cells are also able to release the enzyme in the extracellular space. We determined the release of acetylcholinesterase by cultured dorsal root ganglia neurons at various developmental stages: acetylcholinesterase release is significantly increased at day E20, as compared to younger stages, and 90% of the enzyme released is G4.

Acetylcholinesterase↗
PubMed1987

Motoneurone survival and neurite regeneration requirements: the role of dorsal root ganglion cells during development.

The effect of dissociated dorsal root ganglion cells on the survival of motoneurones in vitro from differently aged chick embryos has been studied. Homogeneous cultures of motoneurones were prepared from 5-8-day embryos, using a cell sorter; dorsal root ganglion cells were obtained from 8-day embryos. The survival of motoneurones from 5-day and 6-day embryos was not enhanced above controls by the presence of dorsal root ganglion cells; however, the survival of motoneurones from older embryos was greatly increased, reaching a maximum of over 80% for 8-day embryonic motoneurones. In contrast, the number of motoneurones that had regenerated neurites when co-cultured with dorsal root ganglion cells for 24 h decreased with the motoneurone age at plating, from 51% at 5 days to less than 10% for 7- and 8-day motoneurones. The survival-enhancing effects were probably mediated by cell contact between the motoneurones and processes of the dorsal root ganglion cells: conditioned media from high-density cultures of dorsal root ganglion cells could not be shown to significantly enhance the survival of motoneurones above that of control levels. The possibility that the ganglion cells exert this survival enhancing effect by depolarizing the motoneurones was examined by exposing 8-day sorted motoneurones to 47 mM potassium; this did not effect the survival of the motoneurones relative to control levels. The stage dependency of the survival of motoneurones on different neurotrophic factors and the dorsal root ganglion cell is discussed.

Animals↗
PubMed2006

Molecular cloning and characterization of beta-expansin gene related to root hair formation in barley.

Root hairs are specialized epidermal cells that play a role in the uptake of water and nutrients from the rhizosphere and serve as a site of interaction with soil microorganisms. The process of root hair formation is well characterized in Arabidopsis (Arabidopsis thaliana); however, there is a very little information about the genetic and molecular basis of root hair development in monocots. Here, we report on isolation and cloning of the beta-expansin (EXPB) gene HvEXPB1, tightly related to root hair initiation in barley (Hordeum vulgare). Using root transcriptome differentiation in the wild-type/root-hairless mutant system, a cDNA fragment present in roots of wild-type plants only was identified. After cloning of full-length cDNA and genomic sequences flanking the identified fragment, the subsequent bioinformatics analyses revealed homology of the protein coded by the identified gene to the EXPB family. Reverse transcription-PCR showed that expression of HvEXPB1 cosegregated with the root hair phenotype in F2 progeny of the cross between the hairless mutant rhl1.a and the wild-type Karat parent variety. Expression of the HvEXPB1 gene was root specific; it was expressed in roots of wild-type forms, but not in coleoptiles, leaves, tillers, and spikes. The identified gene was active in roots of two other analyzed root hair mutants: rhp1.a developing root hair primordia only and rhs1.a with very short root hairs. Contrary to this, a complete lack of HvEXPB1 expression was observed in roots of the spontaneous root-hairless mutant bald root barley. All these observations suggest a role of the HvEXPB1 gene in the process of root hair formation in barley.

Base Sequence↗
PubMed1998

Effects of ion channel activity on development of dorsal root ganglion neurons.

Studies of mouse dorsal root ganglion neurons in vitro demonstrate that ion channel function and regulation can influence a wide range of developmental processes. The work suggests that much as exposure to different trophic factors, the pattern of impulse activity a neuron experiences can have significant structural and functional effects during development. Studies concerning effects of ion channel activity on growth cone motility, axon fasciculation, synaptic plasticity, myelination, and intracellular signaling pathways regulating gene expression are presented in the context of changes in endogenous firing patterns during development.

Action Potentials↗
PubMed1997

A paracrine effect for neuron-derived BDNF in development of dorsal root ganglia: stimulation of Schwann cell myelin protein expression by glial cells.

Addition of neurons to cultures of non-neuronal cells derived from quail embryonic dorsal root ganglia causes a 2.5-fold increase in the proportion of cells that express the glial marker Schwann cell myelin protein (SMP) when compared to cultures devoid of neurons. This effect is mediated by BDNF because incubation with a trkB immunoadhesin that sequesters BDNF, but not with trkA or trkC immunoadhesins, abolishes this stimulation. This neuronal activity can be mimicked by treatment with soluble BDNF that stimulates specifically the conversion of SMP-negative glial cells into cells that express this phenotype. That BDNF is the endogenous neuron-derived factor affecting glial development is further supported by the observation that BDNF is extensively expressed in developing sensory neurons of the avian ganglia both in vivo and in vitro, but not by the satellite cells. These results show for the first time a paracrine role for neuronal BDNF on differentiation of peripheral glial cells. This effect of BDNF is likely to be mediated by the p75 neurotrophin receptor because: (1) p75 immunoreactive protein is expressed by a subset of satellite cells; (2) neutralization of p75 abolishes the BDNF-induced stimulation; (3) a treatment of non-neuronal cell cultures with equimolar concentrations of either soluble NGF or NT-3 also affects the proportion of cells that become SMP-positive. Whereas NGF stimulates the acquisition of this glial antigen to a similar extent as BDNF, NT-3 inhibits its expression, suggesting that distinct neurotrophins signal differentially through p75. These findings also suggest that the definitive phenotype of peripheral glia is determined by a balance between positive and inhibitory signals arising in adjacent neurons.

Animals↗
PubMed2026

Habitat-Adapted Fungal Symbionts Promote Salt Stress Tolerance Through Distinct Root Mechanisms and Shared Shoot Regulatory Networks in Arabidopsis thaliana.

Salinity is a major constraint to crop productivity. Beneficial plant-fungus interactions represent a promising strategy to enhance stress resilience. Here, we investigated fungal endophytes isolated from the roots of Oryza sativa cultivated in saline-prone marshlands of the Guadalquivir River, Spain. From a collection of 38 isolates, five salt-tolerant strains exhibiting plant growth-promoting activity were identified, including a previously uncharacterized Reticulascus sp. strain S5. Co-cultivation assays with the non-native host plant Arabidopsis thaliana demonstrated that S5 increased the root and shoot biomass under salt stress. To elucidate the underlying molecular mechanisms, a comprehensive RNA-Seq analysis of the roots and shoots under control and saline conditions was performed. Fungal colonization induced pronounced transcriptomic changes, particularly in the shoots, including rewiring of the auxin- and abscisic acid-related pathways and the induction of genes associated with cell wall remodeling. Concurrently, defense-related processes, including glucosinolate biosynthesis and ethylene signaling, were broadly repressed, suggesting attenuated stress perception in colonized plants. In the roots, S5 inoculation suppressed the expression of genes involved in root hair development and cell wall organization, indicating a fungus-driven reconfiguration of root development. Moreover, comparative analysis with Fusarium sp. K-23, a fungus that has previously been demonstrated to promote plant growth under salinity stress, revealed distinct root-associated mechanisms but convergence on a shared regulatory module in shoots involving ABA-responsive transcription factors and osmotic stress regulators. Collectively, our findings demonstrate that Reticulascus sp. S5 enhances plant salt stress tolerance through the coordinated transcriptional reprogramming of growth, hormone signaling, and stress responses, highlighting a possible potential of habitat-adapted endophytes for sustainable crop improvement.

Arabidopsis↗
PubMedDate not supplied

The development of rat dorsal root ganglia in three experimental media containing Landry-Guillain-Barré, alcoholic peripheral neuropathy or normal adult sera.

Development of rat dorsal root ganglia in vitro mimics the development of rat dorsal root ganglia in vivo when grown in medium containing human cord serum. When serum from patients either in the acute phase of Landry-Guillain-Barré syndrome or with alcoholic peripheral neuropathy is substituted for human cord serum, maturation is inhibited. Development of fetal relationships occurs between the Schwann cells and axons and many axons become enclosed within the processes of a single Schwann cell. Formation of myelin does not occur. The substitution of normal adult blood for cord serum also inhibits maturation, but unlike development with neuropathic serum, the Schwann cells and axons do not become associated and myelination does not occur.

Alcoholism↗
PubMed1994

Nuclear and cytoplasmic Ca2+ signals in developing rat dorsal root ganglion neurons studied in excised tissue.

Confocal microscopy and the Ca(2+)-sensitive fluorescent dye fluo-3 were used to study subcellular Ca2+ signals in embryonic, neonatal, and adult dorsal root ganglion (DRG) neurons in excised dorsal root ganglia. Optical images obtained from isolated whole embryonic and neonatal ganglia revealed a marked variability in the resting Ca2+ signals of different neurons as compared to signals in adult neurons which were uniformly faint. Many of the embryonic and neonatal neurons displayed nuclear Ca2+ signals at rest which were larger than those in the cytoplasm. Embryonic DRG neurons showed a significant increase in nuclear and cytoplasmic fluorescence in response to depolarization with elevated extracellular potassium or electrical stimulation. A single brief electrical stimulus was sufficient to elicit nuclear Ca2+ signals in a subset of the embryonic neurons. The depolarization-induced Ca2+ signals were blocked by removal of extracellular Ca2+, but not by treatment with 2,5-di (tert-butyl)-1,4 benzohydroquinone (DTBHQ), a compound which depletes intracellular Ca2+ stores. The intensity of the depolarization-induced Ca2+ signals declined significantly between the late embryonic (E18-E20) and early postnatal time periods (P0-P1). The nuclear and cytoplasmic Ca2+ signals of the embryonic DRG neurons in the excised tissue preparation occur at a time of intense target innervation, suggesting a role for Ca2+ signals in the development and maturation of rat DRG neurons.

Aniline Compounds↗
PubMed2003

The influence of wisdom tooth impaction on root formation.

UNLABELLED: The development of wisdom teeth occurs within a definite period of time. The correlation of wisdom tooth development and chronological age has been frequently used for several purposes, e. g. the start of orthodontic treatment, estimates of age or, for legal and anthropological purposes. Wisdom teeth are frequently impacted in the bone. It has been argued that the impaction of wisdom teeth can cause a delay in root development. This thesis could have bearing on age estimations in teenagers and young adults and the timing of dental treatment. The aim of this study was to determine whether the impaction of wisdom teeth influences the velocity of root formation. MATERIAL AND METHODS: The dental x-rays (orthopantomograms) of 1053 outpatients were evaluated (age: 14 to 24 years). The condition "impacted" or "not impacted" was registered for each wisdom tooth present. The impaction types were further classified. The data were then calculated using statistical tools. RESULTS: The correlation between the developmental stages of wisdom teeth and the chronological age is high. However, neither any impaction type nor the whole group of retained wisdom teeth showed a statistically significant different course of root development compared to the root development of non-retained wisdom teeth. DISCUSSION: This study provides evidence for the lack of bearing of wisdom teeth topography on the growth stages of the dental roots within definite time intervals, based on the evaluation of orthopantomograms only. Obviously, the variations of root formation in the selected chronological periods are greater than the variations attributable to possible periods of delay in root formation of retained wisdom teeth. These results could have some importance for the fields of dental anthropology and dentistry.

Humans↗
PubMed2005

LeRALF, a plant peptide that regulates root growth and development, specifically binds to 25 and 120 kDa cell surface membrane proteins of Lycopersicon peruvianum.

A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8x10(-9) M, typical of known peptide hormone-receptor interactions in both plants and animals. The (125)I-azido-LeRALF, when exposed to UVB light in the presence of the cells, strongly labeled only two proteins of 25 kDa and 120 kDa, with the 25 kDa protein being more strongly labeled than the 120 kDa protein. The cell-surface localization of the interaction was indicated, as suramin, a known inhibitor of peptide-receptor interactions, and native LeRALF peptide competed with (125)I-azido-LeRALF labeling of both proteins. Two biologically inactive LeRALF analogs were not competitors. Incubation of (125)I-azido-LeRALF with suspension cultured cells in the dark, where it was fully active, could subsequently be totally dissociated from cells by acid washes, indicating that it was interacting at the cell surface and was not internalized. The (125)I-azido-LeRALF-labeled 25 kDa and 120 kDa proteins could not be solubilized from cell membranes by methods that release peripheral proteins, indicating that they are integral membrane components. The cumulative kinetic and biochemical evidence strongly indicates that the two proteins may be components of a LeRALF receptor complex.

Cell Wall↗