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PubMed1990

[A rapid diagnosis for periodontitis based on enzymatic activity of periodontopathic bacteria. Correlation between enzymatic activity, clinical periodontal parameters, and subgingival level of microorganisms].

There have been various laboratory methods for the microbiological diagnosis of periodontal disease. However, there have been some disadvantages in these methods. In this study of the application of a chair-side test for microbiological diagnosis, the activity of peptidases in periodontal pockets of patients was examined by using the assay system SK-013. SK-013 consists of synthetic substrates and is capable of rapidly (in 15 min) evaluating the activity of specific peptidase from Treponema denticola and Bacteroides species. Using SK-013, we evaluate the correlation between the enzymatic activity, clinical periodontal parameters and subgingival level of microorganisms, including phase contrast microscopy. We calculated the sensitivity and efficacy of SK-013 as a diagnostic indicator in the presence of Spirochetes and in periodontitis. A positive correlation were demonstrated between enzymatic activity, clinical periodontal parameters, the numbers of total cell count, Spirochetes, and M & S ratio. SK-013 was highly sensitive and efficacious (sensitivity: 92%, efficacy: 96%). We concluded that the assay system SK-013 is a useful chair-side method for diagnosing periodontal disease.

Bacteroides↗
PubMed1991

Effect of systemic doxycycline administration on the subgingival microbial flora: a dark-field microscopy study.

The effect of systemically administered doxycycline hydrochloride on subgingival microbial plaque morphotypes was evaluated using dark-field microscopy. After 1 week of drug administration, while the percentage of coccoid bacteria showed a significant increase, the percentages of spirochetes, motile rods and non-motile rods tended to decrease. Since spirochetes and/or motile rods are considered to be associated with the pathogenesis of periodontal disease, the significant decrease in the proportions of these bacteria are considered to be important. Therefore, it is suggested that doxycycline may be beneficial for the treatment of periodontal disease as an adjunct to traditional therapeutic methods.

Adult↗
PubMed2005

Immune evasion of the Lyme disease spirochetes.

The Lyme disease spirochetes, Borrelia burgdorferi sensu lato, have adapted very well to both surviving and persisting in the mammalian host despite a strong host antibody response. It appears that both temporal and spatial regulation of outer surface proteins have contributed to this persistence. The spirochetes are able to bind fH and FHL-1 to their surface, resulting in decreased complement activation. In addition, the organisms have taken advantage of components of tick saliva to aid in their initial immune evasion and dissemination. Studies leading to these conclusions are reviewed here.

Animals↗
PubMed1991

Surface hydrophobicity of small oral spirochetes.

The purpose of the present study was to determine the surface hydrophobicity of small oral spirochetes and to receive an impression of the molecular organization of the cell surfaces. Nine spirochete strains with one endoflagellum from each cell-end (1:2:1 spirochetes) and eight with two endoflagella from each cell-end (2:4:2 spirochetes) from subgingival plaque were examined. Two hydrophobicity assay systems were used: 1) two-phase partitioning and 2) salting-out aggregation by ammonium sulfate. The influence of heat, enzymes, saliva, and serum were examined. The 2:4:2 spirochetes were significantly more hydrophobic than the 1:2:1 spirochetes. For all strains heat treatment increased surface hydrophobicity, whereas incubation with proteases decreased surface hydrophobicity for the 1:2:1 spirochetes. The 2:4:2 spirochetes were unaffected by the proteases. Lipase and hyaluronidase affected the two morphotypes in opposite directions. Saliva did not affect the surface hydrophobicity of any of the strains, whereas rabbit serum decreased this property for the 2:4:2 strains.

Bacterial Adhesion↗
PubMed1986

Erythema chronicum migrans in Sweden: clinical manifestations and antibodies to Ixodes ricinus spirochete measured by indirect immunofluorescence and enzyme-linked immunosorbent assay.

26 Swedish patients with erythema chronicum migrans (ECM) were studied regarding associated clinical symptoms and antibodies to Swedish Ixodes ricinus spirochete. 11/26 (42%) of the patients had associated symptoms, compared to more than 90% of 314 American patients with ECM, as described by Steere et al. Only 2/26 (8%) had multiple skin lesions, compared to 48% of the American patients. Elevated erythrocyte sedimentation rate and circulating immune complexes were demonstrated in 6/25 (24%) and 8/25 patients (32%), respectively, as against in 53% and 84%, respectively, of the American patients. The antibody response to Ixodes ricinus spirochete was measured by indirect immunofluorescence (IFA) and enzyme-linked immunosorbent assay (ELISA). Compared to the 95% percentile of controls, significantly high antibody titers were demonstrated in 3/25 (12%) by IFA, and 7/25 (28%) by ELISA. The ELISA antibody titers differed significantly (p less than 0.05) between ECM-patients and controls. The spirochetal antibody response in ECM was also compared with that in spirochete-associated disease of the central nervous system.

Adolescent↗
PubMedDate not supplied

Correlative light and scanning electron microscopy of intestinal giardiasis, cryptosporidiosis, and spirochetosis.

Cases of intestinal giardiasis, spirochetosis, and cryptosporidiosis were examined by scanning electron microscopy (SEM) using hematoxylin and eosin (H&E)-stained tissue sections from which the coverslips were removed and the sections coated with gold. The technique is simple and reliably provides excellent morphologic detail that is preserved in the deparaffinized 4 microsections. We present examples of nine intestinal parasites examined in this manner and compare this technique with standard H&E staining and special stains with regard to relative costs, turnaround time, labor input, and morphologic preservation. Scanning electron microscopy is a useful adjunct in providing confirmatory evidence in the diagnosis of intestinal giardiasis, spirochetosis, and cryptosporidiosis.

Biopsy↗
PubMed1985

Bacterial chemotaxis: biochemistry of behavior in a single cell.

Bacterial chemotaxis is a primitive behavioral system that shows great promise for being amenable to a description of its molecular mechanism. In Gram-negatives like Escherichia coli, addition of amino acid attractant begins a series of events, starting with binding to certain intrinsic membrane proteins, the MCPs, and ending with a period of smooth swimming. Immediately, methyl-esterification of these MCPs begins and continues during this period. By contrast in the Gram-positive Bacillus subtilis, demethylation of MCPs occurs during the same period. At least two other mechanisms for mediating chemotaxis toward the attractants oxygen and phosphotransferase sugars exist in E. coli, and in these, changes in methylation of MCPs plays no role. Moreover, chemotaxis away from many repellents by B. subtilis appears to involve different mechanisms. Many of the repellents include drugs and toxicants, many of them man-made, so that chemoreceptors could not have specifically evolved; yet the bacteria are often exquisitely sensitive to them. Indeed, the B. subtilis membrane seems to act like a generalized antenna for noxious membrane-active substances.

Adenosine Triphosphate↗
PubMedDate not supplied

Nonsyphilitic spirochetosis in second-trimester fetuses.

Four female fetuses (17-23 weeks) spontaneously aborted by young women (15-19 years old) showed spirochetal microorganisms predominantly in the intestinal lumen and mucosa and to a much lesser extent in other organs. Fetal tissues showed a brisk lymphocytic-plasmacytic response in intestinal mucosa, lungs, and meninges in some cases. In all instances the placenta had chorioamnionitis and severe chronic villitis, with villous vasculitis in some. One fetus had a concomitant cytomegalovirus infection. The observed lesions were reminiscent of Treponema pallidum infections; however, the spirochetes were morphologically different by light and ultrastructural microscopy from T. pallidum and did not react with a silver-enhanced, gold-labeled anti-T. pallidum antibody. In addition, serologic tests for syphilis of the women before or after the abortions were nonreactive. On the basis of clinical pathologic considerations as well as the absence of immunostaining, it is possible also to rule out infections caused by Lyme and relapsing fever Borrelia, Leptospira, and Campylobacter. The spirochetes' prominent tropism for the intestinal tract raises the possibility of a congenital infection with gastrointestinal spirochetal species described in recent years. The placental findings suggest an ascending transamniotic infection, with initial colonization of the intestinal tract and systemic dissemination of the organisms in the fetus and placental villi.

Adolescent↗
PubMed1985

Isolation of the Lyme disease spirochete from mammals in Minnesota.

Lyme disease spirochetes were isolated from the kidneys of two Peromyscus spp. trapped in Minnesota in September and October 1983. No spirochetes were isolated from white-tailed deer (Odocoileus virginianus), red backed voles (Clethrionomys gapperi), or shrews (Sorexy cinereus and Blarina brevicauda). This is the first report of the isolation of the Lyme disease spirochete from the midwestern United States and isolations from these animals, which were free of ticks, suggest that the Lyme disease spirochete may persist in animal organs for months.

Animals↗
PubMed2003

Relapsing fever-like spirochetes infecting European vector tick of Lyme disease agent.

To determine whether relapsing fever-like spirochetes associated with hard ticks may infect Ixodes ricinus ticks in central Europe, we screened questing ticks for 16S rDNA similar to that of Asian and American relapsing fever-like spirochetes. We compared the prevalence of these spirochetes to that of Lyme disease spirochetes transmitted by the same vector. Relapsing fever-like spirochetes infect 3.5% of questing vector ticks in our three central European sites near the Rhein Valley. These spirochetes differ genetically from their American and Asian analogs while being relatively homogeneous in the region we sampled. The Lyme disease genospecies most commonly detected in central Europe are distributed broadly, whereas those that are less frequently found appear to be place-specific. The absence of co-infected ticks suggests that relapsing fever-like and Lyme disease spirochetes may not share hosts. Exposure risk for relapsing fever-like spirochetes is similar to that of certain Lyme disease genospecies. Although many persons may be bitten by ticks infected by relapsing fever-like spirochetes, health implications remain unknown.

Animals↗
PubMed2004

Borrelia burgdorferi persists in the brain in chronic lyme neuroborreliosis and may be associated with Alzheimer disease.

The cause, or causes, of the vast majority of Alzheimer's disease cases are unknown. A number of contributing factors have been postulated, including infection. It has long been known that the spirochete Treponema pallidum, which is the infective agent for syphilis, can in its late stages cause dementia, chronic inflammation, cortical atrophy and amyloid deposition. Spirochetes of unidentified types and strains have previously been observed in the blood, CSF and brain of 14 AD patients tested and absent in 13 controls. In three of these AD cases spirochetes were grown in a medium selective for Borrelia burgdorferi. In the present study, the phylogenetic analysis of these spirochetes was made. Positive identification of the agent as Borrelia burgdorferi sensu stricto was based on genetic and molecular analyses. Borrelia antigens and genes were co-localized with beta-amyloid deposits in these AD cases. The data indicate that Borrelia burgdorferi may persist in the brain and be associated with amyloid plaques in AD. They suggest that these spirochetes, perhaps in an analogous fashion to Treponema pallidum, may contribute to dementia, cortical atrophy and amyloid deposition. Further in vitro and in vivo studies may bring more insight into the potential role of spirochetes in AD.

Aged↗