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Liquid chromatographic determination of cholecalciferol in rodent baits.

Cholecalciferol (vitamin D3) is extracted in acetonitrile on a Goldfisch apparatus, diluted to volume, and determined by reverse phase liquid chromatography (LC). The sum of the peak heights of pre-vitamin D3 and cis-vitamin D3 is used for quantitation. The method was tested for precision, linearity, and recovery. Quadruplicate analyses of 5 formulation samples gave relative standard deviations of 1.56-2.65%. Linearity was excellent with regression and correlation coefficients of 0.9997 and 0.9998, respectively. Recovery was 98.0 +/- 2.7%. The method is applicable to 0.075% cholecalciferol rodent baits.

Cholecalciferol↗

Diaphragmatic paralysis after organophosphate poisoning. A case report.

Peripheral neuropathy has been described as a rare complication of organophosphate toxicity in man. A case of diaphragmatic paralysis occurring after ingestion of malathion in a suicide attempt is reported. The patient required ventilatory support for 3 months during which time there was slow improvement in the diaphragmatic weakness, which by 6 months had resolved completely.

4-Hydroxycoumarins↗

[Control of harmful mammals].

In the Netherlands, several millions of injurious small mammals are killed yearly. This concerns not only mice and rats but also moles, musk-rats and several other species of rodent. At the request of the Netherlands Society for the Protection of Animals, studies were done on the methods in killing these animals, the effectiveness of these methods and the possibilities of reducing the harm done to the animals. The most important results of these investigations are reported in the present paper.

Animal Welfare↗