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Reaction of the avian respiratory system to intratracheally administered avirulent Salmonella typhimurium.

Chickens were inoculated intratracheally (IT) with the SR-11 Salmonella typhimurium deletion mutant x4062 strain. Data collected for 8 days postinoculation (PI) were: signs of respiratory and gastrointestinal disease; histological lesions; the influx, phagocytic proportion, and phagocytic capacity of avian respiratory phagocytes (ARPs); and the proportion of granulocytes vs. macrophages in the lung tissues and lavage fluids of the lungs and air sacs. S. typhimurium-inoculated chickens had no clinical signs of gastrointestinal or respiratory disease but had various degrees of inflammatory changes in the lungs. At 5 hr PI, S. typhimurium-inoculated chickens had approximately 53-fold more ARPs than mock-inoculated controls. Between 26 hr and 8 days PI, the number of ARPs from S. typhimurium-inoculated birds was not significantly higher than the number from the mock-inoculated controls. Flow cytometric analysis of ARPs demonstrated that the proportion of phagocytic ARPs and the phagocytic capacity of ARPs from S. typhimurium-inoculated chickens were significantly higher between 5 and 26 hr PI than those of the ARPs from mock-inoculated chickens. Kinetic changes over 8 days in the granulocyte/macrophage ratios in the lavage fluids, as compared with kinetic changes in the lung tissues, suggested that the granulocytes generally represent a much higher proportion of the ARPs, and egress earlier and in much larger numbers from the tissues to the lumen of lungs and air sacs than do macrophages.

Animals↗

A checklist of helminths from the respiratory system and gastrointestinal tracts of African Anatidae.

A literature survey revealed that 72 helminth species, including 14 known only to the generic level, had been reported from the digestive or respiratory tracts of 28 species of Anatidae in Africa. Most of the digeneans and nematodes reported, were cosmopolitan species that occur in a range of hosts. However, two groups of cestodes, one consisting of cosmopolitan or Eurasian species and the other consisting of species restricted mainly to sub-Saharan Africa, were apparent. A host-parasite list and a detailed parasite-host list provide the synyonomies related to African records, the host and geographical distribution of each species, and the authority and country of origin for each record.

Animals↗

Cloning and expression analysis of a 5HT7-like serotonin receptor cDNA from mosquito Aedes aegypti female excretory and respiratory systems.

In the mosquito Aedes aegypti, 5-HT changes the endogenous rhythm of contractions in the female hindgut and increases fluid secretion in the larval Malpighian tubule. The role of 5-HT as a diuretic hormone in adults has been questioned. We cloned a cDNA encoding a serotonin receptor from a female A. aegypti Malpighian tubule library that is similar to the 5-HT7 receptor from Drosophila melanogaster. The transcript was localized in the tracheolar cells associated with the female Malpighian tubules but no signal was detectable in the tubule epithelium. Immunohistochemistry with specific antibodies confirmed the receptor expression in tracheolar cells and hindgut, and western blots of these tissues showed the expected 50 kDa band. The results suggest a role for serotonin in respiration and that this receptor may coordinate the tubule-hindgut response to serotonin during diuresis.

Aedes↗