Microfilariae in bone marrow aspiration smears.
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The brain tissues of microfilaraemic animals, Mastomys natalensis, which were earlier inoculated (s.c.) with Brugia malayi infective larvae (100 each) were examined for the occurrence of Mf. This was done by staining squash preparations of the brain tissues which were cleared off from the vascular piamater. Animals with blood Mf count of 50 >/per 20 cu. mm were found to harbour Mf in the brain tissues. The Mf count in the brain varied from 5-86/81 cu. mm (sum of Mf detected in 3 tissue pieces, each of 27 cu. mm collected from 3 parts of the brain, viz., the cerebral hemispheres, cerebellum and medulla oblongata). Teh presence of Mf in the brain was confirmed by its detection in 20-micrometers-thick cryosections of the tissue. Also, fine needle aspirates of cerebral hemispheres of an animal showed live Mf.