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At least 937 records · Page 52Linked to original sources
PubMed1990

High level expression of the two outer capsid proteins of bluetongue virus serotype 10: their relationship with the neutralization of virus infection.

DNA representing RNA segments 2 and 5 of bluetongue virus (BTV) serotype 10, corresponding to the genes that code for the outer capsid proteins VP2 and VP5, have been inserted into a baculovirus transfer vector in lieu of the coding region of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV). After co-transfection of Spodoptera frugiperda cells with wild-type AcNPV DNA in the presence of the recombinant transfer vector DNAs polyhedrin-negative recombinant baculoviruses were recovered. When S. frugiperda cells were infected with these recombinant viruses proteins of similar size and antigenic properties to BTV VP2 and VP5 were synthesised. The recombinant VP2, but not the recombinant VP5, was shown to be capable of inducing antibodies that neutralized the infectivity of BTV-10 in vitro.

Antibodies, Viral↗
PubMed1992

Promoter influence on baculovirus-mediated gene expression in permissive and nonpermissive insect cell lines.

The activities of viral and insect promoters were examined in a range of insect cell lines permissive and nonpermissive for the replication of the baculovirus Autographa californica nuclear polyhedrosis virus. Recombinant baculoviruses were constructed to place the bacterial chloramphenicol acetyltransferase gene under the control of promoters strongly active in the early, late, or very late stages of virus replication. In fully permissive cells, expression from a very late promoter was 2- to 3-fold higher than expression from a late promoter and 10- to 20-fold higher than expression from an early promoter or from a virus-borne insect promoter. In cell lines that do not support the efficient production of viral progeny, late-promoter-driven expression was similar to or surpassed very late promoter-driven expression. In nonpermissive insect cell lines, expression driven by an insect promoter derived from Drosophila melanogaster was higher than expression from the three viral promoters and was especially high in the Drosophila cell line tested. Surprisingly, late-promoter-driven expression, which is dependent on DNA replication, was higher than early-promoter-driven expression in three of four nonpermissive lines. In contrast, very late promoter-driven expression was quite limited in nonpermissive cell lines. The results indicate that the promoter used to drive foreign-gene expression strongly influences the range of insect cells which can efficiently support the production of the foreign protein during infection with recombinant baculoviruses.

Animals↗
PubMed1990

Dengue 3 virus infection of Aedes albopictus and Aedes aegypti: comparison of parent and progeny candidate vaccine viruses.

DEN-3 parent (strain CH53489) and progeny candidate vaccine (clone 24/28) viruses were compared in their abilities to interact with Aedes aegypti and Ae. albopictus. The parent and progeny virus were equivalent in their ability to infect, to replicate in, and to be transmitted by both species of mosquitoes. The candidate vaccine DEN-3 clone was temperature sensitive and had small plaque morphology. These phenotypic markers remained stable during mosquito passage. Thus, temperature sensitivity and small plaque morphology are not reliable biological markers for attenuation.

Aedes↗