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Results of artificial insemination of horses in Poland in the post-war period.

Artificial insemination (A.I.) of mares in Poland has not yet been widely applied. Initial attempts were made by research groups between 1945 and 1955 but A.I. of mares was only introduced into the normal practice of A.I. Centres during 1964-67. Intensive research into methods for preserving stallion semen in liquid nitrogen has been undertaken since 1968. Of the total of 3734 mares artificially inseminated in Poland since 1945, 350 were inseminated with frozen semen. The slow progress of A.I. in horses is imputed to the small numbers of people involved in the work, to the conservation of the authorities responsible for breeding, to the wrong choice of stallions for A.I. Centres and, most importantly, to the lack of a simple and reliable method of preservation of stallion semen.

Animals↗

Hysteroscopic insemination of small numbers of spermatozoa at the uterotubal junction of preovulatory mares.

Mares were inseminated with motile spermatozoa suspended in 30-150 microliters Tyrode's medium directly onto the uterotubal papilla at the anterior tip of the uterine horn, ipsilateral to the ovary containing a dominant preovulatory follicle of > or = 35 mm in diameter, by means of a fine gamete intrafallopian transfer (GIFT) catheter passed through the working channel of a strobed light videoendoscope. Insemination of 10, 8, 25, 14, 11 and 10 mares with, respectively, 10.0, 5.0, 1.0, 0.5, 0.1 or 0.001 x 10(6) motile spermatozoa resulted in conception rates of, respectively, 60, 75, 64, 29, 22 and 10%. Deposition of 1.0 x 10(6) motile spermatozoa onto the uterotubal papilla began to approach the limit of successful fertilization. These doses are far lower than the 3-15 x 10(9) spermatozoa normally ejaculated by fertile stallions during mating, and the accepted minimum dose of 500 x 10(6) spermatozoa used for conventional uterine body insemination in mares. The simplicity of the technique offers a practical means of exploiting new breeding technologies that require very small numbers of spermatozoa in horse breeding.

Animals↗

[Psychological problems in heterologous insemination].

Heterologous insemination is being established in increasing numbers in Anglo-Saxon, middle and north European countries, although some medical, legal and theological-ethical aspects are still not settled. Our experiences with this type of sterilisation in regard to marriage stability and psycho-physical development of the children are positive. During the months of insemination, even with psychically balanced couples, conflicts arise such as disturbed vita sexualis (sexual life) emotional stress situations or aggravation from the husband's inferiority complex which can endanger the success of the treatment or even the marriage. The doctor in charge of insemination has the responsibility to discuss and analyse arising problems with the couple so that both husband and wife are emotionally and intellectually prepared when pregnancy occurs.

Affective Symptoms↗

Effect of acrosomal defects on fertility of bulls used in artificial insemination and natural breeding.

Four bulls that produced spermatozoa with a high percentage of abnormal acrosomes were individually placed in pens with females for 21 days. Frozen semen from 2 of the bulls was used for artificial insemination. One of the bulls was placed in a competitive mating situation with normal bulls at pasture. First service pregnancy rates were determined by transrectal ultrasonography 28 days after bull removal from breeding pens, or after the last artificial insemination. The results of competitive mating at pasture were determined from breeding observations, the phenotypic characteristics of calves sired, and blood typing for parentage. The results of these studies suggest that bulls that produce a high percentage of spermatozoa with indented acrosomes may have normal fertility when used in artificial insemination or in single sire mating; however, their fertility may be low when breeding competitively with bulls with normal spermiograms.

Acrosome↗

[Combined artificial insemination and synchronisation of oestrus in sheep (author's transl)].

A brief introduction is followed by a description of artificial insemination in sheep, based on a review of the literature. A number of experiments are then discussed, which were mainly concerned with the possibilities arising when artificial insemination and synchronisation of oestrus are combined. It is concluded that satisfactory results (70-80 per cent pregnant) may be obtained, provided a large number of live spermatozoa (500 x 10(6)) are inseminated at the right time.

Animals↗

[Insemination of female rabbits].

The author's observations revealed that out of 20 female rabbits inseminated with undiluted semen, 18 animals got pregnant (90 per cent of the total). The insemination time ranged from 25 minutes to 5 hours and 30 minutes after the coitus with the sterile male. The volume of the insemination dose indispensable for pregnancy ranged from 0,2-0,6 ml, and the number of the spermatozoa with normal motility in that dose ranged from 27-30 million.

Animals↗

[A prospective randomized trial of the impact of misoprostol (PgE1) on pregnancy rate after intrauterine insemination (IUI) therapy: a preliminary report].

Although the use of prostaglandin plays an important role in the reproductive human physiology, it is still controversial in the reproductive field. Ovarian stimulation as well as intrauterine insemination increased the reproductive goals in certain group of patients. The objective of the present study was to evaluate the prostaglandin effect (misoprostol) in patients under ovarian stimulation and intrauterine insemination and their final outcome in the clinical pregnancy rate. There were a total of 59 ovarian stimulated cycles, the study group (n = 29) received 200 micrograms of prostaglandin E1 (misoprostol) intravaginal after IUI, compared with the control group (n = 30). Demographic characteristics were similar in both groups. There were no differences in age, FSH. LH and E2, hCG day and number of ampoules between groups. However, a significant pregnancy rate was observed between groups (31% study group vs. 20% control group). We concluded that prostaglandin application in stimulated cycles under intrauterine insemination remain a beneficial effect showing in the pregnancy outcome.

Administration, Intravaginal↗

[Adaptation of a cervical cap for human artificial insemination].

The authors have designed a cervical cap in plastic throught which artificial insemination can be practised. The material allows prolonged contact between semen and the cervical mucus. Tests carried out 24 hours after insemination have shown that after 159 inseminations there were mobile spermatozoa in the mucus in 145 cases.

Female↗

Amino acid turnover by elongating cattle blastocysts recovered on days 14-16 after insemination.

Blastocyst elongation from day 14 to day 16 after insemination coincides with a major phase of embryo loss in cattle. Protein synthesis, reflected in protein content, increases markedly over this period but little is known about the amino acid requirement of elongating blastocysts at this time. Cattle blastocysts produced in vivo were recovered on days 14-16 after insemination and cultured individually for up to 8 h in synthetic oviduct fluid containing a physiological mixture of amino acids plus 1 mmol glutamine l(-1) and 0.1% (w/v) polyvinyl alcohol (SOFaaPVA). After 1, 4 and 8 h in culture, an aliquot of culture medium was removed and the rate of amino acid depletion or production was calculated per unit of protein and per hour of culture. Amino acids were depleted or produced at different rates. Arginine was depleted from the medium at a significant rate (P < 0.05) during all culture periods. Alanine and glutamate were produced at a significant rate (P < 0.05) during all culture periods. The rate of alanine production was significantly greater (P < 0.05) in blastocysts recovered on day 14 compared with days 15 or 16 after insemination. Alanine production and arginine depletion tended to be greater in smaller embryos recovered on day 14 compared with larger and later stage embryos, indicating that earlier stage embryos may have higher metabolic activity than later stage embryos. Qualitatively, the pattern of amino acid consumption and production during elongation was similar to that shown from the zygote to early blastocyst stage.

Alanine↗

Frequency of multiple inseminations in field-collected Anopheles gambiae females revealed by DNA analysis of transferred sperm.

We investigated the frequencies of single and multiple matings in field-collected female Anopheles gambiae by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple inseminations. Polyandry was found in six of 239 females examined, or 2.5% of the samples. Previous analyses of the molecular form of the sperm and female extracts using a PCR-based diagnostic procedure showed that two of these multiple inseminations involved cross-mating between two chromosomal/molecular forms of An. gambiae s.s. Thus polyandry occurred within-form in 1.7% of examined females while other multiple inseminations may be linked to processes of reproductive isolation between forms of An. gambiae.

Animals↗

Prognostic factors for successful outcome in patients undergoing controlled ovarian stimulation and intrauterine insemination.

OBJECTIVE: To determine the prognostic factors associated with successful outcome following controlled ovarian stimulation and intrauterine insemination. DESIGN: Retrospective analysis. SETTING: University-based assisted reproductive technology centre, Hong Kong. PATIENTS AND METHODS: Patients included 292 couples undergoing 600 treatment cycles, following a standard protocol of human menopausal gonadotrophin injections. Multiple logistic regression analysis was performed to determine which demographic and sperm parameters gave the maximum discrimination to predict pregnancy. RESULTS: One hundred and eleven pregnancies resulted from treatment. The pregnancy rates were 18.5% per cycle and 37.9% per couple. The age of the women was significantly lower for pregnant cycles, and the serum oestradiol levels and number of follicles greater than 16 mm in diameter were significantly higher, compared with non-pregnant cycles. The sperm concentration and number of motile spermatozoa were also significantly increased in pregnant cycles. Pregnancy rate was significantly increased when the raw semen sample contained 20 million/mL or more spermatozoa, normal forms comprised 7% or more, and when the number of motile spermatozoa in inseminated samples was 1 million or greater. CONCLUSION: Using multiple logistic regression analysis, age of the women and serum oestradiol level had the maximum power to predict pregnancy following ovarian stimulation and intrauterine insemination.

Adult↗

[Superovulation and intrauterine insemination in treatment of idiopathic infertility in 202 cycles].

OBJECTIVE: To evaluate the effect of superovulation with recombinant follicle stimulating hormone (r-FSH) therapy and intrauterine insemination in the treatment of idiopathic infertility. METHODS: Superovulation with r-FSH therapy and intrauterine insemination were used in 202 cycles of 88 couples in the Department of Obstetrics and Gynecology of Monash Medical Centre. RESULTS: The per cycle ovulation rate and in-ovulation rate were 95.7% and 4.3% respectively, and the per cycle pregnancy rate was 11.6% with no cases of hyperstimulation. The cancelling rate was 7.4% because of the development of multiple follicles. The overall cumulative conception rate was 22.7% per patient, with 15% of twin pregnancies. There were no differences between pregnancy group and non-pregnancy group in age, BMI, treatment days, number of mature follicles, endometrial thickness and number of treatment cycles. The only significant parameter observed between the two groups was infertility time (P < 0.05), which was longer in non-pregnancy group [(30.52 +/- 13.08) months] than in pregnancy group [(24.25 +/- 6.45) months]. CONCLUSIONS: Superovulation and intrauterine insemination is a safe and more cost-effective method in treatment of idiopathic infertility.

Adult↗

The socio-legal acceptance of new technologies: a close look at artificial insemination.

Heated debates often surround the introduction of an important new technology into society, as exemplified by current controversies surrounding human cloning and privacy protection on the Internet. Underlying these controversies are disruptions to central socio-legal values caused by these new technologies. Whether new technologies will eventually be accepted by society is often contingent on the reaction of the legal system. This mandates the formulation of a conceptual framework for understanding and structuring the way the law should react in cases surrounding the adoption of new technologies. By using the case study of artificial insemination this Article develops the tools for structuring the legal role in the acceptance process of new technologies. The three-century controversy surrounding the innovation of artificial insemination results from the innovations' disruption of the socio-legal value of the family. Artificial Insemination--although invented in the eighteenth-century--was rarely used until the 1930s, and only legalized in the 1960s. Its application to surrogacy and its use by unmarried women extends the controversy into the twenty-first century. The case study demonstrates the nature of the relationship among the technological, social and legal acceptance processes of new technologies, and analyzes the legal acceptance debate. The conceptual framework produced is useful in understanding and structuring the legal role in current debates surrounding the introduction and acceptance of new technologies.

Attitude↗

Pregnancy by insemination of cryopreserved spermatozoa from a man with retrograde ejaculation: a case report.

BACKGROUND: Owing to the prevalence of diabetes mellitus, spinal injuries and aggressive surgical treatment of cancer, the number of younger patients with retrograde ejaculation is increasing. Since medical treatment to restore antegrade ejaculation often fails, several options for accomplishing insemination by these patients, including the use of sperm-rich urine obtained after masturbation and in vitro fertilization with sperm retrieved from the seminal tract, have been reported. We used the least invasive and most inexpensive procedure in a patient/couple with this condition. CASE: A 23-year-old man suffered from retrograde ejaculation after a spinal injury. He could achieve erection and engage in sexual intercourse but seldom had an orgasm or the sensation of ejaculation. We obtained spermatozoa from urine produced after masturbation at home and froze them. We used these frozen-thawed spermatozoa for intrauterine insemination, leading to the term birth of a healthy infant. CONCLUSION: In selected patient/couples, frozen spermatozoa obtained from postmasturbation urine can be used successfully for intrauterine insemination. This minimally invasive and most inexpensive procedure should be tried before planning in vitro fertilization.

Adult↗

[Investigation of correlative factors affecting successful intrauterine insemination].

OBJECTIVE: To investigate the main correlative factors affecting the pregnancy rate of intrauterine insemination (IUI) by means of analyzing 186 patients and 216 cycles of IUI. METHODS: Comparisons were made between different pregnancy rates from five respects: infertility reasons, stimulation and natural cycles, different protocols for stimulating ovulation (CC group, CC + hMG group, hMG group), two methods for semen treatment (swim-up and gradient centrifugal), and insemination time (endometrial thickness and E2 levels at the day of hCG injection, and times of IUI). RESULTS: The pregnancy rates resulting from male and cervix factors (29.82%, 25.00%) were significantly higher than those from other factors. There were few statistical differences in the pregnancy rates between stimulation and natural cycles (19.54% vs 16.67%), between the three different protocols for stimulating ovulation (CC 6.50%, CC + hMG 14.28%, hMG 22.30%), between gradient centrifugal and swim-up methods (20.13% vs 16.13%) and between one-time IUI and two-time IUI (19.73% vs 17.39%) (P > 0.05). However, the pregnancy rates were statistically correlated with the endometrial thickness at the day of hCG injection and different E2 levels of the follicles over 14 mm in diameter (P < 0.05). CONCLUSION: IUI is of more applied value for infertility caused by male and cervix factors. With these factors in view, to choose the most suitable insemination time is the key to increasing pregnancy rate.

Estradiol↗

[Insemination from HIV-positive males].

INTRODUCTION: Discordant couples, in which the man is HIV positive and the woman is HIV negative, face limited options when they wish to produce healthy children whilst practicing safe sex. In order to conceive they must abandon unprotected sex, which carries high risk of HIV infection to the woman. OBJECTIVE: To develop a method for removing the HIV from semen and then perform intra uterine insemination, following verification of the HIV-negative spermatozoa fraction by PCR. METHODS: Motile spermatozoa were isolated from semen samples by the gradient and "swim-up" techniques. HIV-RNA was tested before and after the procedure in both the semen and the purified spermatozoa fraction. Insemination was performed on ovulation day only when the absence of any viral particles was verified by PCR. RESULTS: Four couples underwent a total of 8 cycles of intra uterine insemination (IUI). Presently, two healthy babies were born. The two mothers and newborn were found to be HIV negative following the delivery. CONCLUSION: We report a safe procedure which enables biological parenthood for HIV-1 discordant couples without the risk of infecting the female partner. Our clinical data corroborate with the collaborative European data on this subject.

Adult↗

Minimal endometriosis and intrauterine insemination: does controlled ovarian hyperstimulation improve pregnancy rates?

Fifty normally menstruating women staged laparoscopically as having minimal endometriosis were given an option to be treated with intrauterine insemination with or without ovarian stimulation. Twenty-five patients had unmedicated natural cycles (ie, no medication for follicular stimulation), and 25 underwent controlled ovarian hyperstimulation. All subjects received hCG to time ovulation, followed 36 hours later with intrauterine insemination. Sixteen pregnancies, eight in each group, resulted from a total of 132 cycles. The cumulative probabilities of conception for the first, second, and third cycles were 0.13, 0.26, and 0.38 in the natural-cycles group and 0.12, 0.25, and 0.34 in the medicated-cycles group, respectively (P greater than .05). Monthly fecundity was 0.14 for the natural-cycles group and 0.13 for the medicated-cycles group (P greater than .05). We conclude that there is no difference in pregnancy rates or monthly fecundity over a 3-month period with controlled ovarian hyperstimulation as opposed to natural cycles combined with intrauterine insemination.

Endometriosis↗

Seminal hyperviscosity is associated with poor outcome of controlled ovarian stimulation and intrauterine insemination: a prospective study.

OBJECTIVE: To determine the relationship between seminal hyperviscosity and pregnancy outcome in patients undergoing ovarian hyperstimulation and intrauterine insemination (IUI). METHODS: Patients were enrolled in the study between October 2002 and December 2003 at the Toronto Centre for Advanced Reproductive Technology. This was a prospective trial that included 37 infertile couples with abnormal seminal viscosity who underwent 57 insemination cycles as treatment for infertility (group I) and 37 couples undergoing 51 IUI cycles during the same time period, with normal semen viscosity, who served as controls (group II). Cycles were stimulated using either gonadotropin (FSH) only or FSH combined with an aromatase inhibitor, and raw semen processed for intrauterine insemination using swim-up or density gradient. Results-The mean number (+/-SD) of IUI cycles was 1.93 +/- 1.42 per patient (range 1 to 7 cycles) in group I and 2.4 +/- 1.05 (range 1 to 4) in group II. The overall pregnancy rate (PR) was 14% (8/57) and 11.8% (6/51) per cycle, and 21.6% (8/37) and 16.2% (6/37) per patient in group I and in group II, respectively. Among patients where the male had seminal hyperviscosity, five (62.5%) pregnancies miscarried in the first trimester; there was no miscarriage in the control group. CONCLUSION: The high miscarriage rate in couples with semen hyperviscosity may be attributed to biophysical alterations or chemical changes of the ejaculate that could impact sperm quality despite normal sperm parameters on semen analysis.

Adult↗