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[Forensic medical examination in cases of sexual abuse].

The article presents the analysis of forensic medical expert practice in the field of sexual abuse. Drawbacks in expert examinations, official decisions on such examinations, low expertise of forensic medical personnel in sexual crime, lack of tools for genital and anal examination are analysed and methods of correction of this situation are proposed.

Anal Canal↗

[Detection and investigation of microparticles of biological tissues in gunshot wounds].

We studied feasibility of expert purposes solution in examination of gunshot bullet injuries by investigation traces of microparticles of tissues expulsed from the wounds and settled near the victim. Laws of such traces formation are established, feasibility of localization of the place of wounding, of the shot direction finding, definition of the gender, group, species of bioobject is shown.

Firearms↗

[Fatal pulmonary thromoembolism].

INTRODUCTION: Fatal pulmonary thromboembolism is a relatively common autopsy finding. The aim of this investigation is to describe the magnitude of the problem in an autopsy material and factors of prophylactic importance and to discuss the importance of the autopsy. MATERIAL: Deceased with lethal pulmonary thromboembolism diagnosed by forensic autopsy at the Department of Forensic Medicine at the University of Southern Denmark in 1992-2005, in total 58 individuals. Data were collected from autopsy- plus police reports and medical records. METHOD: Descriptive case-only investigation. RESULTS: The pulmonary embolism had in none of the cases been diagnosed before death. Two of the individuals in this investigation were healthy young females who used oral contraceptives, and one was a psychiatric patient who died from pulmonary embolism after five days of physical restraint. CONCLUSION: The diagnosis is sometimes not made before the autopsy. The decreasing autopsy frequency may result in a lack of diagnosis of pulmonary embolism as cause of death.

Adolescent↗

[Analysis of deaths caused by rail-vehicles in the materials collected by the Department of Forensic Medicine in Bydgoszcz in the years 1992-2002].

The analysis focused on cases of death following accidents involving rail-vehicles, in which autopsies were performed at the Department of Forensic Medicine in Bydgoszcz in the years 1992-2002. Sixty-five individuals died in train accidents (80%), 16 people (20%) were victims of tram accidents (20%). Males constituted 86% of the material, while females accounted for 14%. The highest number of rail-vehicle accidents was observed in 1992. The most common reason of death was multiorgan injury. In the subgroup of tram accidents, the authors noted no injuries of the highest severity, such as limb amputations, amputations or crushing of the head, or fragmentation or rupturing of the trunk. On the other hand, in railroad accidents, the percentage of the above injuries was 26%, 44% and 24.5%, respectively. The most severe injuries were noted in the group of people hit by a train while in prone position.

Academic Medical Centers↗

[Phylogeographic approach in the interpretation of mitochondrial DNA sequencing results in forensics].

In recent years, forensic mitochondrial DNA analysis has been undertaken from an evolutionary perspective. In particular, the phylogeographic approach based on a phylogenetic analysis of the spatial distribution of mitochondrial haplotypes and haplogroups appears to be a useful tool in the interpretation of identification cases. In this study, the phylogeographic approach has been employed in the analysis of three difficult forensic cases, where single nucleotide, homoplasmic differences were found between the reference and evidentiary haplotypes. mtDNA sequence variation has been examined by the control region (HVS I and HVS II) direct sequencing. Additionally, in order to clarify the subhaplogroup status of the selected haplotypes, DNA sequences of entire mitochondrial genomes obtained from two samples representing J1b subclade have been analyzed.

DNA Fingerprinting↗

[Medico-legal autopsy case of an infant suffering anaphylactic shock during dental treatment. Potential hazards in the use of a rubber-dam-sheet for infants].

We report a medico-legal autopsy of a 4-year-old girl without any history of allergies, who died following anaphylactic shock due to local anesthesia with lidocaine during dental treatment. The shock symptoms were overlooked due to a rubber-dam-sheet covering her face. The autopsy revealed laryngeal edema, secretory fluid filling the trachea, severe pulmonary congestion and edema. An immunohistochemical investigation showed numerous mast cells releasing histamine in lungs, and the fluids in the pulmonary alveoli and veins also showed positive staining. In addition, the plasma concentration of histamine in the heart blood showed a high value. From these findings, the cause of death was determined to be respiratory failure due to anaphylactic shock induced by lidocaine. In this case, her death was considered to be due to medical malpractice; adequate life support had not been performed, because anaphylactic shock was overlooked until the rubber-dam-sheet removed. In addition, due to two previous autopsy case reports that described infantile fatalities involved with the misusage of a rubber-dam-sheet for the patients under local anesthesia, we therefore recommend immediate improvements in rubber-dam-sheets and/or better applications of them in pediatric dentistry.

Anaphylaxis↗

[Spontaneous ruptures of the urinary bladder in the routine forensic examination].

This article analyses three cases of death following a spontaneous rupture of the urinary bladder. One case is based on an extensive tamponade of the bladder eight days after a transurethral resection of the prostate gland. Two other cases of death by spontaneous rupture resulted from increased alcohol consumption. The paper presents an overview of pathomorphological findings such as the typical intraperitoneal rupture localisation at the posterior wall of the urinary bladder or bladder dome and the subsequent diagnosed causes of death. In addition predisposing (anamnestic) influencing factors such as chronic alcoholism are highlighted and their relevance for the clinical urologist and the forensic pathologist are discussed.

Adult↗

[Microscopic myocardial changes and their significance for forensic medical diagnosis].

Morphological myocardial changes essential for diagnosis in forensic medical medicine are listed as well as alterations in the vessels of the myocardium, cardiac muscular fibers and cardiomyocytes important for diagnosis of death of ischemic heart disease, acute alcohol poisoning, alcohol cardiomyopathy, closed cardiac lesions. Changes induced by reflex impacts on the heart and postmortem alterations are also shown.

Cardiomyopathies↗

[The role of histological examination in timing of the splenic injury].

Basing on her findings at forensic-histological investigations of splenic injuries, the author describes histological inflammatory-reparative alterations in splenic tissue in the rupture regions regarding time parameters. The investigations allow prescription of splenic damage.

Abdominal Injuries↗

[Assessment of accumulation of the shot products on the targets in shooting from a short-barreled gun with a muffler for estimation of shooting distance].

A practical expert task--to estimate shot distance and order of shots made in two victims from a gun with muffler--is described as illustration of opportunities of the complex investigation with experimental shots and emission spectral analysis of the targets. Distribution of the shot soot on the targets in shooting from the distance up to 1 m is analyzed.

Firearms↗

[Identification of tsunami victims in Thailand: international experience in organization and conduction of forensic-medical expert investigations in emergency situations with many victims].

Russians were among the victims of tsunami in Thailand. The authors participated in the activity of Thai Tsunami Victim Identification Center as experts for identification of tsunami victims. The article outlines management and course of identification, some results. The unique experience with international cooperation in conduction of forensic-medical expert investigations in emergency situations with many victims is discussed.

DNA↗

Characteristics of urinary tract tumours in the area of Balkan endemicnephropathy in Croatia.

There is a general consensus that the incidence of urothelial tumours is much higher among the inhabitants of areas of Balkan endemic nephropathy than in populations in nonendemic areas. Data from different authors on tumour incidences in unrelated endemic areas in various countries vary widely, however. Lack of understanding of the possible etiological relationship between Balkan endemic nephropathy and the increased incidence of urothelial tumours justifies efforts to conduct retrospective studies using unique diagnostic criteria. In order to investigate the occurrence of urinary tract tumours in Slavonski Brod, a part of which has a high incidence of nephropathy, a retrospective study was carried out using data and tissue samples collected at the Department of Pathology and Forensic Medicine of the Slavonski Brod Medical Centre. During the last 16 years, 193 urinary tumours from the county were examined; the material available consisted of autopsy reports and surgical and biopsy specimens. Tumours were diagnosed histologically and coded according to guidelines of the WHO and US registries, as papillomas, papillary and solid carcinomas of the transitional-cell type, squamous-cell carcinomas and carcinomas in situ. A 5.1 times higher incidence of urothelial tumours was seen among inhabitants of the nephropathic area of the county than in nonendemic areas. Tumours from cases in the endemic region also tended more often to be malignant and multiple; they were not infrequently bilateral.

Adult↗

Release of tissue paraquat into formalin solution during fixation.

Formalin-fixed tissues and formalin solutions are among the most frequently found materials in pathology and forensic science laboratories. However, these materials are seldom used for the identification of poisons for forensic toxicology purposes. In this study, the possibility that paraquat may be released from formalin-fixed tissues during the fixation process was investigated. However, because of the interference of formaldehyde on the reduction of paraquat with dithionite reagent, paraquat in formalin solutions was treated with ion-pair column chromatography and then determined by measuring the derivative spectrum of reduced paraquat. The results show that the interference of formalin on paraquat determination has been eliminated by the proposed method. Furthermore, a study on the formalin solutions of fixed organs in cases with suspected paraquat intoxication revealed that portions of tissue paraquat had been released into formalin during the fixation process. Moreover, the paraquat levels in formalin increased with increased storage time. Therefore, these data suggest that the combined concentrations of paraquat in the formalin-fixed tissues and formalin solutions might reflect more reliably the total paraquat in the postmortem tissues. This investigation could be of value to the forensic toxicologist, especially in cases in which no fresh tissue samples are available for analysis.

Adult↗