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Genetic factors regulate processes related to anxiety in mice.

The propensity for anxiety-related behavior has been studied by comparing two highly inbred strains of mice, ABP/Le and C57BL/6ByJ, in two behavioral procedures, open-field and light-dark preference. Their Mendelian F2 population allowed us to evaluate the putative involvement of four easily identifiable loci in anxiogenic processes. In fact, chromosomal regions containing the brown, pink-eyed dilution and short-ear loci on the 4th, 7th and 9th chromosomes respectively are associated with anxiety-related behavior patterns. In addition, binding of [3H]flumazenil to brain GABA(A) receptors was measured as a biochemical index that may be associated with observed behavior patterns.

Animals↗

Manual laterality in haptic and visual reaching tasks by tufted capuchin monkeys (Cebus apella). An association between hand preference and hand accuracy for food discrimination.

Manual laterality was examined in 26 tufted capuchins (Cebus apella) in three tasks differing in their sensorimotor demands and the availability of visual cues. The Haptic discrimination task required the monkeys to discriminate haptically between two pumpkin seeds and two tinfoil items stuck into a tray inside an opaque box. The other two tasks required the monkeys to reach for two pumpkin seeds stuck into the tray within a transparent box with vision (Visually guided reaching task) or without vision (Visual-Tactual reaching task) during reaching. A significant group-level left hand bias was found for food retrieval in both the Haptic discrimination and Visual-Tactual tasks, and a significant group-level right hand bias in the Visually guided reaching task. The strength of hand preferences did not differ among the tasks. It was found that the accuracy of food recognition in the Haptic discrimination task was greater for the left than the right hand. The results suggest that the differences in the manipulo-spatial requirements of the tasks and in the availability of visual cues can variously affect manual laterality in capuchins. The left-hand preferences for the Haptic discrimination and Visual-Tactual tasks as well as the left-hand advantage for food discrimination may reflect a greater involvement of the right hemisphere in processing haptic information.

Animals↗

"Try it. You'll like it." Effects of information on willingness to try novel foods.

Three experiments are reported on the effects of "taste" or nutrition information on willingness to try novel foods. "Taste" information improved responses to four out of the five foods examined. There was a consistent, but not statistically significant, trend for nutritional information to be effective. In experiment I, conducted with 3- to 8-year-old children in a laboratory setting, and in experiment II, conducted with 10- to 20-year-olds in a cafeteria, there were strong age effects. Older subjects responded more positively to novel foods than did younger subjects. There were no significant interactions between information and age and there were no sex differences. It is commonly assumed that novel foods are rejected because they are thought to be dangerous. However, the fact that dangerous foods are good tasting should be irrelevant to willingness to taste them. Our results are consistent with the idea that, in settings like laboratories and cafeterias, culture has already defined foods as being safe. Perhaps rejection in such settings is based on fear of a negative sensory experience.

Adolescent↗

Postnatal development of lateralized motor preference in the African grey parrot (Psittacus erithacus).

The parrot appears to provide a potentially unique animal model of handedness in humans, but few (if any) observational studies of early postnatal development of postural/motor asymmetries have been published. We studied three African Grey hatchlings, raised without human physical contact, for the first 5 months of life. All three animals failed to show consistent postural and/or motor asymmetries until the end of the 4 postnatal week. These results appear to be comparable to data from prior studies with human infants. Delayed development of lateral motor and/or postural preferences may represent an evolutionarily adaptive strategy for altricial animals.

Animals↗

Regional central nervous system densities of delta-opioid receptors in alcohol-preferring P, alcohol-nonpreferring NP, and unselected Wistar rats.

The densities of delta-opioid receptors in the central nervous system of alcohol-naive, adult, male, alcohol-preferring P, alcohol-nonpreferring NP, and Wistar rats were examined with the use of quantitative autoradiography. Slides with coronal 20-microm sections through the regions of interest were incubated in 5 nM [3H]-[D-Pen(2),D-Pen(5)]enkephalin (DPDPE) to label delta(1)-opioid receptor sites. Nonspecific binding was determined in the presence of 10 microM naloxone. Significant differences between the P and the NP rat lines were found in numerous cortical regions, the basolateral amygdala, and the posterior hippocampus, with 10%-20% lower [3H]-DPDPE binding found in the P line. In most regions examined, binding levels in the Wistar rats were intermediate between those of the P and the NP rats. Significantly lower [3H]-DPDPE binding levels in the P rat may indicate fewer delta(1)-opioid receptors or decreased binding affinity. The lower binding in certain limbic regions, such as the basolateral amygdala and posterior hippocampus, as well as cortical differences in the P rat may be associated with the divergent alcohol drinking behaviors found between the P and the NP lines.

Alcohol Drinking↗

Toward a better understanding of the influences on physical activity: the role of determinants, correlates, causal variables, mediators, moderators, and confounders.

BACKGROUND: For research on physical activity interventions to progress systematically, the mechanisms of action must be studied. In doing so, the research methods and their associated concepts and terminology become more complex. It is particularly important to clearly distinguish among determinants, correlates, mediators, moderators, and confounder variables used in physical activity research. This article examines the factors that are correlated with and that may have a causal relationship to physical activity. METHODS AND RESULTS: We propose that the term "correlate" be used, instead of "determinant," to describe statistical associations or correlations between measured variables and physical activity. Studies of the correlates of physical activity are reviewed. The findings of these studies can help to critique existing theories of health behavior change and can provide hypotheses to be tested in intervention studies from which it is possible to draw causal inferences. Mediator, moderator, and confounder variables can act to influence measured changes in physical activity. Intervening causal variables that are necessary to complete a cause-effect pathway between an intervention and physical activity are termed "mediators." The relationship between an intervention and physical activity behaviors may vary for different groups; the strata by which they vary are levels of "moderators" of the relationship. Other factors may distort or affect the observed relationships between program exposure and physical activity, and are known as "confounders." CONCLUSIONS: Consistent use of terms and additional research on mediators and moderators of intervention effects will improve our ability to understand and influence physical activity.

Behavioral Sciences↗

Preferred drinking locations of Scottish adolescents.

A sample of Scottish schoolchildren (N=1240) were asked a series of questions about their consumption of alcoholic beverages. Those who had consumed alcohol were asked about the circumstances of their last drinking occasion. That is, what alcoholic drink(s) they consumed, how they obtained this alcohol and where they were when they drank it. This was done with the intention of quantifying the extent of under-age drinking in different settings. The results presented in this paper indicate a continuum of drinking styles between what might be regarded as low-risk and high-risk circumstances. At one extreme, much drinking tended to take place within the family home, usually under parental supervision. At the opposite extreme, other alcohol consumption took place in a variety of public or 'hidden' outdoor locations. Consumption in these latter location types more often resulted in intoxication. Also, more dangerous, high alcohol, large volume beverages (e.g. white cider) tended to be consumed in more risky locations. The implications of these findings are discussed in terms of the potential harm and delinquency that can be associated with adolescent drinking.

Adolescent↗

The role of contextual and personal factors in children's risk taking.

The primary aims of the present studies were to (a) add to the sparse developmental database on risk taking and (b) conduct initial tests of a self-regulation model (SRM) of risk taking. According to the SRM, inappropriate risk taking is associated with overconfidence, falling prey to dysregulating influences (e.g., impulsivity, peer presence, etc.), and an insensitivity to outcomes. Experiment 1 tested these proposals by assessing the effects of peer presence and 4 personal factors on the risk taking of 3rd, 5th, and 7th graders. Results generally supported the predictions of the SRM. In Experiment 2, the SRM gained further support from the finding that 5 variables correlated with risk taking in 4th, 6th, and 8th graders: ability beliefs, a preference for thrill seeking, peer nomination, competitiveness, and interest. The discussion focuses on the meaning of age and gender differences in risk-taking as well as the interventional implications of the SRM.

Age Factors↗

Children's interactions in triads: behavioral profiles and effects of gender and patterns of friendships among members.

In all, 56 triads of same-sex 3rd-, 4th-, and 5th-grade children were observed during an intimate discussion, a cooperative puzzle task, a competitive game, and free play. Observers coded triadic interaction using a Q sort and individual process ratings. Of particular interest was how gender and the pattern of friendship ties among group members related to children's behavior. Triads of girls were more intimate, exchanged more information, and were less aggressive than were triads of boys. Analyses of within-gender variability revealed 2 prototypical types of triads among boys but only 1 among girls. Girls and boys expressed similar attitudes toward triadic interaction in postsession interviews. However, girls', but not boys', attitudes were closely linked to the quality of interactions during the session. For both sexes, interaction was not strongly influenced by the initial configuration of friendship ties among triad members.

Age Factors↗

Rule selection versus rule execution in preschoolers: an error-detection approach.

In 2 experiments, an error-detection approach was used to determine whether 3-year-olds' perseverative errors on the postswitch phase of the Dimensional Change Card Sort (DCCS) are due to lack of response control or representational inflexibility. In Experiment 1, 3-, 4-, and 5-year-olds watched a puppet sort perseveratively on the postswitch phase and evaluated its responses. Most 4- and 5-year-olds detected the puppet's perseverative errors, whereas most 3-year-olds failed to do so despite detecting errors on a simpler card sort. Experiment 2 revealed that 3-year-olds who failed to correctly evaluate the puppet's behavior tended to fail their own DCCS. Results imply that perseveration on the DCCS cannot be attributed to difficulty inhibiting prepotent motor responses. Instead, changes in rule use between 3 and 5 years of age are interpreted in terms of the development of representational flexibility.

Age Factors↗

Children's use of mental state information in selecting persuasive arguments.

Whether and when children use information about others' mental states to invent or select persuasive strategies were examined. In Study 1, preschoolers, 3rd-graders, and 6th-graders (ns = 11, 12, and 16, respectively; 17 girls) were told about story characters' persuading parents to buy pets or toys. Children were either given or not given information about story parents' beliefs and asked to invent or select appropriate arguments. Older children, but not preschoolers, used belief information to select arguments. Results were replicated in Study 2 (16 kindergartners, 16 3rd-graders; 19 girls). In Study 3, kindergartners and 1st-graders (N = 16; 6 girls) reasoned well on false-belief tasks but not on persuasion tasks, suggesting that failure to consider mental states in persuasion was not due to lack of a belief concept. Findings suggest that mental state understanding may continue to develop after the preschool years; methodological qualifications are also considered.

Child↗

The formation of in-group favoritism and out-group prejudice in young children: are they distinct attitudes?

Although standardized measures of prejudice reveal high levels of ethnocentric bias in the preschool years, it may reflect in-group favoritism or out-group prejudice. A measure that partially decouples the two attitudes was given to White children between 4 and 7 years of age to examine the reciprocal relation between and the acquisition and correlates of in-group and out-group attitudes. The two attitudes were reciprocally correlated in 1 sample from a racially homogeneous school but not in a 2nd sample from a mixed-race school. In-group favoritism did not appear until 5 years of age but then reached significant levels; it was strongly related to developing social cognitions. Out-group prejudice was weaker, but its targets suffer from comparison with the high favoritism accorded in-group members.

Attitude↗

Self-stimulating rats combine subjective reward magnitude and subjective reward rate multiplicatively.

For rats that bar pressed for intracranial electrical stimulation in a 2-lever matching paradigm with concurrent variable interval schedules of reward, the authors found that the time allocation ratio is based on a multiplicative combination of the ratio of subjective reward magnitudes and the ratio of the rates of reward. Multiplicative combining was observed in a range covering approximately 2 orders of magnitude in the ratio of the rates of reward from about 1:10 to 10:1) and an order of magnitude change in the size of rewards. After determining the relation between the pulse frequency of stimulation and subjective reward magnitude, the authors were able to predict from knowledge of the subjective magnitudes of the rewards and the obtained relative rates of reward the subject's time allocation ratio over a range in which it varied by more than 3 orders of magnitude.

Action Potentials↗

Differential effects of postingestive feedback on the reward value of sucrose and lateral hypothalamic stimulation in rats.

The effects of postingestive feedback on the value of intraoral sucrose and lateral hypothalamic (LH) stimulation were compared. Rats chose between a fixed LH stimulation train or a compound reward composed of the same stimulation train and an oral infusion of 1 M sucrose. The rats preferred the compound reward when postingestive feedback was reduced by opening a gastric cannula. However, when the cannula was closed, the compound was preferred only at the beginning of each session, and preference declined or reversed as consumption continued. A second experiment showed that the reduction in preference caused by closing the gastric cannula was not due to a punishing effect of the gastrointestinal load. This study suggests that postingestive signals modulate gustatory reward at a stage of processing before gustatory and brain stimulation rewards are combined.

Animals↗

The development of a conditioned place preference to morphine: effects of microinjections into various CNS sites.

Experiment 1 examined whether microinjections of morphine (1 microg in 0.5 microl over 1 min x 2 pairings) into 13 different CNS sites produced a conditioned place preference (CPP). Injections into the lateral ventricles (LV), ventral tegmental area (VTA), or periaqueductal gray (PAG) produced a CPP; injections 1 mm dorsal to the PAG or VTA, or into the caudate putamen, medial frontal cortex, hippocampus, lateral nucleus of the amygdala, lateral hypothalamus, pedunculopontine tegmental nucleus, posterior hypothalamus, ventral palladium, or nucleus accumbens septi (core or shell) did not. In Experiment 2, morphine 0.2 microg produced a CPP when injected into the VTA but not in the PAG, while 5.0 microg was effective in both sites. The CPP induced by systemic morphine (4 mg/kg x 1 pairing) was blocked by naloxone methiodide (NM) injected (2 nmol in 0.5 microl) into the VTA. PAG injections of 2 nmol reduced, and 5 nmol NM eliminated, the CPP. The results confirm that morphine injections into the VTA or the PAG are rewarding, that blockade of opioid receptors in either site disrupts a morphine-induced CPP, and that the VTA is more sensitive to both effects.

Animals↗

Absence of knowledge attribution and self-recognition in young chimpanzees (Pan troglodytes).

Mirror self-recognition in chimpanzees (Pan troglodytes) is typically delayed until 4 1/2-8 years of age. Also, species capable of mirror self-recognition may be capable of some forms of mental state attribution related to intentions and knowledge. Previous investigations of knowledge attribution by chimpanzees used adolescents and adults but did not explicitly test for self-recognition. We report an investigation of knowledge attribution in 6 young chimpanzees previously tested for self-recognition. Subjects were required to discriminate between a person who had seen where food was hidden and another person who had not. The results are consistent with the proposition that most chimpanzees younger than 4 1/2 years of age show neither mirror self-recognition nor knowledge attribution. The results are also consistent with the idea that, just as in humans, development of self-recognition in chimpanzees may precede development of knowledge attribution.

Aging↗