Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “VACATIONS”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 919 records · Page 51Linked to original sources
PubMed1999

Anatomy of a world cruise.

BACKGROUND AND METHOD: We registered the patient-related activities of a large cruise ship's medical center during a 103-day worldwide voyage and compared data from 694 passengers (53% women) with median age 66 years with data from 540 crew (27% women) with median age 30 years of age. RESULTS: The medical staff had 3033 (1537 crew, 1496 passenger) consultations (=206 consultations per week) and performed 982 diagnostic procedures. Skin disorders dominated in the crew and were more frequent than in the passengers (29% versus 13%, p<.01), respiratory problems dominated in the passengers and were more frequent than in the crew (26% versus 17%, p<.01), while cardiovascular disorders were more common in the passengers (7% versus 1%, p<.01). Forty-six injuries (35 passengers, 11 crew) were recorded. One passenger died aboard. Seventy-six crew (14%) were not able to work for a total of 110 days (1.1 crew sick per day), while five crew were signed off for medical reasons. CONCLUSIONS: To be a doctor or nurse on a large cruise ship during a long sea voyage is no vacation. The medical staff should expect a varied general practice with a higher consultation frequency rate than shoreside, even higher in crew

Adolescent↗
PubMedDate not supplied

Factors associated with injuries occurring aboard vessels in Alaska: differences between residents and nonresidents.

BACKGROUND: Over the past decade, visitors to Alaska have spent increasing amounts of time engaged in water-based recreational activities aboard vessels of various sizes. Serious vessel-related injuries to travelers in Alaska involve not only medical care from unfamiliar health care providers and facilities but also entail the loss of vacation time and the need for injured travelers to return to their homes under less than optimal traveling conditions. METHODS: This study employed a retrospective, case-comparison analysis to identify differences in factors associated with recreational injuries acquired aboard watercraft that resulted in hospitalizations of residents and nonresidents of Alaska during 1991 to 2000. Tests of proportions were conducted to elucidate differences in demographic characteristics and injury precursors between the two subgroups. Specific injury outcomes were then tested for significance using odds ratios. RESULTS: Alaska residents and nonresidents demonstrated significant differences for both demographic factors and factors describing events leading to injuries, and for injury outcomes. Nonresidents were more likely to be 65 years or older, female, and aboard cruise ships when injuries occurred. Nonresidents were more likely to suffer fracture injuries, to suffer injuries with Abbreviated Injury Scores greater than 2, to experience posthospital discharges to sites other than their homes, and to experience postinjury disabilities. CONCLUSIONS: Alaska residents and nonresidents in this study showed significant differences in demographics, precipitating events, and injury outcomes. The findings lend support for targeted safety promotion programs.

Accidents↗
PubMed1990

Comprehensive pediculosis screening programs for elementary schools.

Pediculosis (head lice) is a major school and community health problem with an estimated 6-12 million cases annually resulting in $367 million a year in consumer costs, lost parental wages, and school system expenses. The greatest incidence is seen in children ages 5-12; however, the incidence in the 24-36 year old group is increasing due to their exposure to infested children. Developing comprehensive pediculosis screening programs in elementary schools provides an effective method for preventing epidemics from occurring by accomplishing early detection. These programs also promote primary health care and education among elementary schoolchildren, school officials, educators, and parents. An effective program includes screening the entire school three times a year: mid-September, December, and near spring vacation, and enforcing a "No Nit" policy. Establishing a successful approach to control pediculosis results in decreased incidence and transmission, reduced absenteeism, and financial savings for parents and school districts.

Child↗
PubMed1987

Interaction of transport phenomena and surface reactions.

Phenomena that occur when blood contacts a foreign surface are controlled by transport processes in the bloodstream immediately above the surface and by reactions at the surface itself. All constituents of blood that attach to a surface may leave the surface and be replaced by other constituents. Some of the constituents may attach in superposition. This turnover of constituents at the surface is influenced by the nature of the substrate and the shear stress between the blood and the surface. The turnover process is easy to demonstrate with platelets. When platelets depart from a surface, the site they vacate has a higher attractiveness for adhesion of other platelets than the surrounding surface that has not had a cell attached to it, but this enhanced attractiveness diminishes in a few minutes if a new occupant does not occupy the site. As data accumulate, it is beginning to appear that platelet turnover differs between different blood donors but remains relatively constant for each individual unless dietary or pharmacological changes that affect the turnover rate are introduced.

Blood Circulation↗
PubMed1976

Effects of supervised daily dental plaque removal by children: first-year results.

In September 1973, a three-year school-based study was initiated in East Hampton, Connecticut, a rural nonfluoridated community. The purpose of the study is to determine the effect on dental decay, gingival inflammation and oral hygiene of removing dental plaque through supervised daily toothbrushing and flossing in school during a three-year period. To establish baseline data, three indexes were used: the DHC Index to evaluate gingivitis; the DMF Surface Index to quantify dental caries and the PHP Index to measure dental plaque. The examinations for dental caries, including radiographs, are scheduled annually in September, whereas the plaque and gingival examinations are done biannually, in September and June. After the baseline examinations the 481 children were blocked according to grade and sex and then were randomly assigned to either a treatment group or to a control group. In November, the treatment group was provided 10 sessions of instruction in plaque removal. For the remainder of the year (six months) they practiced daily plaque removal, supervised by a dental hygienist and a nurse. The control group was not instructed in oral hygiene procedures. In the treatment group, mean plaque and gingival scores at the June (first follow-up) examination were 14 percent and 29 percent lower, respectively, than at baseline. No change was seen in the control group. At the second follow-up examination in September (after summer vacation), the same indexes were nearly at baseline levels in the treatment group. There was no treatment effect on dental caries increments after one year of study. Considering the nature of the treatment regimen and the few treatments during the first year of the study, it is not surprising that there were no caries-preventive benefits demonstrated among children in the treatment group after one year. The total number of the plaque removal sessions will be greater during the second year of the study. Therefore, the possibility of detecting a caries preventive effect at the twenty-four month examinations, should one exist, will be enhanced.

Adolescent↗
PubMed1977

Effects of supervised daily dental plaque removal by children: II. 24 months' results.

After two school years of a supervised daily plaque-removal program in school, children in the treatment group showed a significant reduction in plaque and gingival inflammation scores compared with children in the control group. However, differences between groups in terms of plaque and gingivitis scores virtually disappeared during the summer vacation. The increment of dental caries was lower in the treatment group than in the control group, but the reduction, which averaged about 20 percent, was not statistically significant either for teeth or for surfaces. Although the 24-month findings of the present study indicate some degree of success in reducing plaque and gingivitis and a marginal effect in reducing the incidence of dental caries among children who engaged in daily, supervised plaque-removal in school, obvious drawbacks limit the value of this regimen.

Adolescent↗
PubMed1990

Parental awareness of the emergency management of avulsed teeth in children.

Although public awareness campaigns on avulsed teeth have been mounted in the past, no study has investigated the knowledge parents possess of the protocol for the management of this dental emergency. The purpose of this investigation was to evaluate, by means of a questionnaire, the parental awareness of the emergency management of avulsed teeth in children. Over 2000 parents were surveyed during a four-week period at 20 suburban vacation swimming centres. The results indicated that almost two-thirds of respondents would attempt replantation of an avulsed tooth but further questioning showed they did not know the correct procedures. Thirty-three per cent of respondents were unaware of any after-hours emergency dental services. Ninety-two per cent felt they should seek professional help urgently following an avulsion injury, but their knowledge of transport media for the tooth was poor. Only 5 per cent knew that milk was the medium of choice for both washing and transporting an avulsed tooth. Ninety per cent of parents surveyed had never received advice on what to do in the event of an accident where a permanent tooth was avulsed. This study revealed the need for educational campaigns aimed at parents to increase their knowledge of the emergency procedures required when the tooth is avulsed.

Adult↗
PubMed1972

A study of frog muscle maintained in organ culture.

1. Frog muscles are isolated and maintained in organ culture conditions for periods of up to 2 months. During the first 2 weeks, muscle fibres have normal resting membrane and action potentials. Subsequently the potentials decline in amplitude.2. Slow muscle fibres also survive in culture and retain their ability to give maintained contractures.3. Muscle sensory receptors continue to function in culture until the axon terminals degenerate at about 2 weeks.4. Neuromuscular transmission is normal during the first few days of culture, after which the motor endings degenerate. Transmission persists longer (up to 17 days) if a long segment of nerve is left attached to the muscle. With short-nerve preparations failure of transmission in vivo occurs at about the same time as in culture. With long-nerve preparations failure of transmission is delayed even further in culture.5. In short-nerve preparations miniature end-plate potentials disappear, in general, at about the time that transmission fails. In long-nerve preparations some end-plates continue to have miniature end-plate potential activity for a short time after nerve impulses cease to evoke any response; but eventually miniature potential activity disappears from all end-plates.6. After a few days of electrical silence, miniature end-plate potentials reappear at some of the denervated end-plates. The proportion of denervated end-plates which show miniature end-plate potentials in culture is smaller than in muscles denervated in situ.7. Electron microscopy shows that muscle structure is well preserved in culture, that the axons degenerate and that the Schwann cells move to occupy the space vacated by the axons. The Schwann cells are very probably the source of the acetylcholine which evokes miniature potentials in the denervated end-plates.

Acetylcholine↗
PubMed1982

Denervation changes in normal and myasthenia gravis human muscle fibres during organ culture.

1. Human intercostal nerve-muscle obtained from normal and myasthenia gravis affected patients has been organ cultured for up to 5 weeks at 23 degrees C. In addition normal nerve-muscle has been cultured for up to 2 weeks at 36 degrees C. Muscle fibres had normal resting and overshooting action potentials. Input resistances dropped markedly after 21 days at 23 degrees C or 10 days at 36 degrees C.2. Muscle fibre action potentials became partially resistant to tetrodotoxin (10(-7) g/ml.) after culturing for 8 days at 36 degrees C.3. Extrajunctional acetylcholine (ACh) sensitivity was compared in fresh and cultured muscle. Fresh normal fibres possessed extrajunctional ACh sensitivity covering several hundred micrometres around the end-plate and at the muscle-tendon junction. Myasthenia gravis affected fibres had reduced extrajunctional sensitivity at the end-plate and no detectable ACh sensitivity near the tendon.4. Less than one third of normal muscle bundles showed an increased area of ACh sensitivity after several days in organ culture. Under the same conditions myasthenic muscle bundles did not show an increase in extrajunctional ACh sensitivity,5. M.e.p.p.s were present for 2-4 days in normal fibres cultured at 36 degrees C. In normal and myasthenic muscles cultured at 23 degrees C m.e.p.p.s disappeared after 6-8 days and re-appeared in some fibres (50%) after approximately two weeks in organ culture. These m.e.p.p.s were abolished by curare and increased in frequency by hypotonic solution suggesting they are due to the release of ACh-packets from Schwann cells.6. Electron microscopic examination of cultured human muscle indicates that the disappearance of m.e.p.p.s corresponds with degeneration of nerve terminals.7. In muscle bundles shown to possess m.e.p.p.s after 13-14 days, the synaptic gutter, which had been vacated by the nerve terminal, was usually occupied by a Schwann cell or projections of Schwann cell cytoplasm. This indicates that the Schwann cell at denervated human end-plates may be capable of releasing packets of ACh.8. It is concluded that the organ culture system described here is suitable for studying normal and diseased human muscle fibres. Using this system we find that the denervation changes which follow nerve transection appear to be similar in most respects at normal and myasthenic end-plates.

Action Potentials↗
PubMed1998

Photoaffinity labeling of the benzodiazepine binding site of alpha1beta3gamma2 gamma-aminobutyric acidA receptors with flunitrazepam identifies a subset of ligands that interact directly with His102 of the alpha subunit and predicts orientation of these within the benzodiazepine pharmacophore.

Photoincorporation of ligands into the benzodiazepine site of native gamma-aminobutyric acidA (GABAA) receptors provides useful information about the nature of the benzodiazepine (BZ) binding site. Photoincorporation of flunitrazepam into a single population of GABAA receptors, recombinant human alpha1beta3gamma2, was investigated to probe further the mechanism and orientation of flunitrazepam and other ligands in the BZ binding site. It was concluded that the receptor is primarily derivatized with the entire, unfragmented, flunitrazepam molecule, which undergoes a conformational change during photolysis and largely vacates the benzodiazepine binding site. Investigation of the BZ site after photoincorporation of [3H]flunitrazepam confirmed that binding of other radioligands was unaffected by incorporation of flunitrazepam. This did not correlate with their efficacy but depended on the presence of particular structural features in the molecule. It was observed that affected compounds have a pendant phenyl moiety, analogous to the 5-phenyl group of flunitrazepam, which are proposed to overlap and interact with the same residue or residues in the BZ binding site. Because the major site of flunitrazepam photoincorporation has been shown to be His102, we propose that this group of compounds interacts directly with His 102, whereas compounds of other structural types have no direct interaction with this amino acid. The orientation of ligands within the BZ binding site and their specific interaction with identified amino acids are not well understood. The data in the current study indicate that His102 interacts directly with the pendant phenyl group of diazepam, and further implications for the pharmacophore of the BZ binding site are discussed.

Azides↗
PubMed1992

Response of a protein structure to cavity-creating mutations and its relation to the hydrophobic effect.

Six "cavity-creating" mutants, Leu46----Ala (L46A), L99A, L118A, L121A, L133A, and Phe153----Ala (F153A), were constructed within the hydrophobic core of phage T4 lysozyme. The substitutions decreased the stability of the protein at pH 3.0 by different amounts, ranging from 2.7 kilocalories per mole (kcal mol-1) for L46A and L121A to 5.0 kcal mol-1 for L99A. The double mutant L99A/F153A was also constructed and decreased in stability by 8.3 kcal mol-1. The x-ray structures of all of the variants were determined at high resolution. In every case, removal of the wild-type side chain allowed some of the surrounding atoms to move toward the vacated space but a cavity always remained, which ranged in volume from 24 cubic angstroms (A3) for L46A to 150 A3 for L99A. No solvent molecules were observed in any of these cavities. The destabilization of the mutant Leu----Ala proteins relative to wild type can be approximated by a constant term (approximately 2.0 kcal mol-1) plus a term that increases in proportion to the size of the cavity. The constant term is approximately equal to the transfer free energy of leucine relative to alanine as determined from partitioning between aqueous and organic solvents. The energy term that increases with the size of the cavity can be expressed either in terms of the cavity volume (24 to 33 cal mol-1 A-3) or in terms of the cavity surface area (20 cal mol-1 A-2). The results suggest how to reconcile a number of conflicting reports concerning the strength of the hydrophobic effect in proteins.

Amino Acid Sequence↗
PubMed1971

Synaptic adjustment after deafferentation of the superior colliculus of the rat.

Eyes were removed from rats shortly after birth, when there are few formed synapses in the colliculus. It was found that synaptogenesis continues to give a near-normal ratio of terminals containing either spheroidal or flattened vesicles. After eye removal in adult rats, however, reinvasion of synaptic sites vacated by degenerate optic terminals occurs, with an incomplete return toward a normal proportion of synaptic types.

Animals↗
PubMed1982

Effect of Dietary Extremes on Impala (Aepyceros melampus) Rumen Epimural Flora.

The impala, classified as an intermediate feeder among wild ruminants, grazes exclusively on green grasses during the summer rains but turns to an essentially browse habit during the winter, when a diet of leaves, pods, and twigs is supplemented with dried grasses. Scanning electron microscope studies of bacteria attached to the rumen epithelia of animals feeding on digestible, carbohydrate-rich grazing diet showed a high level of surface colonization, with pockets of mixed morphological types occupying spaces vacated by desquamated epithelial cells. Rumen wall colonization in browsing animals on an indigestible, abrasive, low-plane diet showed denudation of distal papillar surfaces with sparse homogeneous adherent flora in the microprotected areas towards the bases. Forage characteristics associated with raised volatile fatty acid concentrations in ingesta, higher rate of epithelial sloughing, and minimum abrasion were interrelated with density and complexity of the adherent bacterial flora.

Journal Article↗
PubMed1968

Terminal disinfection in hospitals wihh quaternary ammonium compounds by use of a spray-fog technique.

Spray-fogging of hospital rooms with a quarternary ammonium disinfectant was found to be an effective means of reducing the number of detectable airborne and surface bacteria. The level of bacterial contamination in hospital rooms was determined before and after fogging by means of the gravitational fallout method, the petri dish swab technique, and volumetric air-sampling procedures. Rooms vacated by patients infected with staphylococci, streptococci, pseudomonads, and salmonellae were tested and found to be effectively decontaminated of most of the detectable organisms by the fogging procedure.

Air Microbiology↗
PubMed1990

An investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against Streptococcus mutans.

Local oral passive immunization with Streptococcus mutans-specific monoclonal antibody (MAb) (Guy's 13) prevented recolonization by indigenous S. mutans in human volunteers who had first been treated with a conventional antibacterial agent (chlorhexidine). The F(ab')2 fragment of the MAb was as protective as the intact immunoglobulin G, but the Fab fragment of the molecule failed to prevent recolonization of S. mutans. In subjects receiving the MAb Fab fragment, S. mutans levels in dental plaque and saliva reappeared at a similar rate to that found in sham-immunized subjects who received either saline or a nonprotective MAb. In vitro, MAb had no bacteriostatic or bacteriocidal effect on S. mutans. However, S. mutans grown in the presence of either intact immunoglobulin G MAb or the F(ab')2 fragment formed very long chains, which resulted in clumping of the cells. S. mutans grown with either saline or the MAb Fab fragment formed significantly shorter chains, more characteristic of streptococcal growth in liquid media. The results suggest that the two binding sites of the MAb molecule may be an essential feature for preventing streptococcal colonization but that the ability to bind to phagocytes and activate complement which resides in the Fc fragment is not essential. Protection against colonization by S. mutans lasting up to 2 years was observed in immunized subjects, although MAb was applied over a period of only 3 weeks. Furthermore, functional MAb was detected up to 3 days following application of MAb to the teeth. The long-term protection could not be accounted for by a persistence of MAb on the tooth surface, and we have suggested that it may be due to a shift in the balance of the oral flora which discouraged recolonization by S. mutans. However, examination of the proportions of Streptococcus sanguis and veillonella species in the recolonization experiments failed to reveal a significant change in the proportions of either organism, which returned to approximately the preexperimental levels in both the immunized and control groups. These findings confirm the in vivo functional specificity of the MAb to S. mutans but are not consistent with the suggestion that S. sanguis or veillonella take over the niche vacated by S. mutans, unless the shift in the proportion of these organisms cannot be detected by the method used.

Adult↗
PubMed2001

Structures of bacterial flagellar motors from two FliF-FliG gene fusion mutants.

Flagella purified from Salmonella enterica serovar Typhimurium contain FliG, FliM, and FliN, cytoplasmic proteins that are important in torque generation and switching, and FliF, a transmembrane structural protein. The motor portion of the flagellum (the basal body complex) has a cytoplasmic C ring and a transmembrane M ring. Incubation of purified basal bodies at pH 4.5 removed FliM and FliN but not FliG or FliF. These basal bodies lacked C rings but had intact M rings, suggesting that FliM and FliN are part of the C ring but not a detectable part of the M ring. Incubation of basal bodies at pH 2.5 removed FliG, FliM, and FliN but not FliF. These basal bodies lacked the C ring, and the cytoplasmic face of the M ring was altered, suggesting that FliG makes up at least part of the cytoplasmic face of the M ring. Further insights into FliG were obtained from cells expressing a fusion protein of FliF and FliG. Flagella from these mutants still rotated but cells were not chemotactic. One mutant is a full-length fusion of FliF and FliG; the second mutant has a deletion lacking the last 56 residues of FliF and the first 94 residues of FliG. In the former, C rings appeared complete, but a portion of the M ring was shifted to higher radius. The C-ring-M-ring interaction appeared to be altered. In basal bodies with the fusion-deletion protein, the C ring was smaller in diameter, and one of its domains occupied space vacated by missing portions of FliF and FliG.

Artificial Gene Fusion↗
PubMed2005

Sun exposure and risk of melanoma.

BACKGROUND: As skin cancer education programmes directed to children and adolescents continue to expand, an epidemiological basis for these programmes is necessary to target efforts and plan for further evaluation. AIMS: To summarise the epidemiological evidence on sun exposure during childhood and adolescence and melanoma risk. METHODS: A literature review was conducted using Medline (1966 to December 2004) to identify articles relating to sun exposure and melanoma. The review was restricted to studies that included sun exposure information on subjects 18 years of age or younger. RESULTS: Migrant studies generally indicate an increased melanoma risk in individuals who spent childhood in sunny geographical locations, and decreasing melanoma risk with older age at arrival. Individuals who resided in geographical locations close to the equator or close to the coast during childhood and/or adolescence have an increased melanoma risk compared to those who lived at higher latitudes or never lived near the coast. The intermittent exposure hypothesis remains controversial; some studies indicate that children and adolescents who received intermittent sun exposure during vacation, recreation, or occupation are at increased melanoma risk as adults, but more recent studies suggest intermittent exposure to have a protective effect. The majority of sunburn studies suggest a positive association between early age sunburn and subsequent risk of melanoma. CONCLUSION: Future research efforts should focus on: (1) clarifying the relation between sun exposure and melanoma; (2) conducting prospective studies; (3) assessing sun exposure during different time periods of life using a reliable and quantitative method; (4) obtaining information on protective measures; and (5) examining the interrelations between ability to tan, propensity to burn, skin type, history of sunburns, timing and pattern of sun exposure, number of nevi, and other host factors in the child and adolescent populations.

Adolescent↗
PubMed1971

Medical staffing and training in chest diseases.

A survey of 164 area departments of chest diseases in England and Wales presents details of 321 consultants and 176 chest physicians in other career grades. During the past 10 years there have been 99 consultant and 76 senior hospital medical officer vacancies, with 61 and 23 replacements, respectively, of which only 22 and 8 were whole-time in chest diseases. Of the total, 119 consultants and 45 in other career grades are now aged 55 and over.In most chest clinics the slowly diminishing work load from tuberculosis has been more than offset by increasing admission of other chest diseases, usually to fewer beds. Many areas need an increase in senior establishment and many require improved clinical facilities; some need both to bring them into line with official policy based on the recommendations of the Scadding report.During the next five years there will be 52 consultant and 11 other senior career posts in chest diseases vacated through retirement. At least eight consultant appointments a year, in terms of whole-time equivalents, will be mandatory for at least five years and probably for 10 years to maintain existing standards without allowance for essential improvements or the development of regional thoracic centres.Present training schemes in the specialty cannot produce more than two or three trained candidates a year for these eight vacancies. The description of prospects in the specialty as "uncertain" deters young doctors from entering it and discovering how broad a spectrum of medicine it embraces. It is urgently necessary for schemes of training to be set up immediately in conjunction with a considerable increase in training posts at senior registrar level embracing both general and chest medicine.

Adult↗