The pentose cycle. Control and essential function in HeLa cell nucleic acid synthesis.
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Collisional-induced dissociation (CID) mass spectra of the [M + H](+) and [M - H](-) ions obtained under fast atom bombardment conditions of a number of methyl glycoside di-, tri- and tetrasaccharides, containing D-xylopyranosyl and/or L-arabinofuranosyl residues at the non-reducing terminus, do not provide information about their ring size. This information could only be obtained from a careful comparison of the intensity ratio of the [M + Na - 90](+) and [M + Na - 104](+) ions ((0,2)X(t)/(1,5)X(t)) in the high-energy CID spectra of the sodium-cationized di-, tri- and probably also tetrasaccharide compounds.
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The activity of glucose-6-phosphate dehydrogenase changes when the mycelium is grown on different carbon sources. In the presence of ribose, the activity of the enzyme is approximately 3 times higher than when the mycelium is grow in a medium containing glucose. This increase in the enzyme activity is developed progressively when the concentration of ribose increases from 1% to 6% and it is blocked if cycloheximide is added to the medium. This results suggest an increase in "de novo" synthesis of the enzyme rather than an specific activation of the latent enzyme. Three isoenzymes of glucose-6-phosphate dehydrogenase are isolated from mycelium grown on ribose, whereas only two isoenzymes are isolated from mycelium grown on glucose.
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