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Results for “Optical imaging”

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At least 919 records · Page 51Linked to original sources
PubMed2003

Novel bioactive and stable neurotensin peptide analogues capable of delivering radiopharmaceuticals and molecular beacons to tumors.

The prevalence of neurotensin receptor (NTR) in several human tumors makes it an attractive target for the delivery of cytotoxic drugs and imaging agents. Native neurotensin (NT) is a tridecapeptide that binds to NTR and induces tumor growth. Unfortunately, NT has a short plasma half-life, which hinders its use for in vivo biomedical applications. Numerous reports suggest that Arg(8)-Arg(9) and Tyr(11)-Ile(12) amide bonds are particularly susceptible to degradation by proteolytic enzymes. Predicated on this observation, we substituted Arg(8), Arg(9), and Ile(12) amino acids with the corresponding commercially available mimics. These surrogate amino acids are amenable to standard Fmoc peptide synthesis strategy, and the resulting compounds are stable in biological media for >4 h and bind to NTR with high affinity. Furthermore, conjugating DTPA to the new peptides and subsequent labeling with (111)In-DTPA for nuclear imaging or fluorescein for optical imaging did not diminish the NTR binding affinities of the peptides. In vivo biodistribution of a representative (111)In-DTPA-NT peptide analogue in SCID mice bearing NTR-positive human adenocarcinoma (HT29) xenograft shows that the compound was primarily retained in tumor tissue (2.2% ID/g) and the kidneys (4.8% ID/g) at 4 h postinjection. Coinjection of cold NT and the radiolabeled NT peptide analogue inhibited the tumor but not the kidney uptake, demonstrating that retention of the radiolabeled compound in tumor tissue was mediated by NTR specific uptake while it accumulates in the kidneys by a nonspecific mechanism. These findings show that the new NT peptide analogues are robust and can deliver imaging agents to NTR-positive tumors such as pancreatic cancer.

Adenocarcinoma↗
PubMed1998

Application of a video-optical beam imaging system for quality assurance of medical accelerators.

Method validation techniques were developed and experiments were carried out using a beam imaging system (BIS, Wellhöfer Dosimetrie, Schwarzenbruck, Germany) for routine quality assurance of medical accelerators. The routine quality assurance tasks include x-ray beam flatness and symmetry check, light/radiation field congruence test, beam energy constancy for electrons and mechanical checks for couch and collimator rotations. Comparisons were made between the BIS application and conventional quality assurance methods that use radiographic films or detector arrays. In this work, we have demonstrated efficiency and accuracy of the BIS to perform some of the routine quality assurance tasks for medical linear accelerators.

Electrons↗
PubMed2005

Characterization of female breast lesions from multi-wavelength time-resolved optical mammography.

Characterization of both malignant and benign lesions in the female breast is presented as the result of a clinical study that involved more than 190 subjects in the framework of the OPTIMAMM European project. All the subjects underwent optical mammography, by means of a multi-wavelength time-resolved mammograph, in the range 637-985 nm. Optical images were processed by applying a perturbation model, relying on a nonlinear approximation of time-resolved transmittance curves in the presence of an inclusion, with the aim of estimating the major tissue constituents (i.e. oxy- and deoxy-haemoglobin, lipid and water) and structural parameters (linked to dimension and density of the scatterer centres) for both the lesion area and the surrounding tissue. The critical factors for the application of the perturbation model on in vivo data are also discussed. Forty-six malignant and 68 benign lesions were analysed. A subset of 32 cancers, 40 cysts and 14 fibroadenomas were found reliable for the perturbation analysis. For cancers, we show a higher blood content with respect to the surrounding tissue, while cysts are characterized by a lower concentration of scattering centres with respect to the surrounding tissue. For fibroadenomas, the low number of cases does not allow any definite conclusions.

Breast Cyst↗
PubMed2006

Texture analysis of speckle in optical coherence tomography images of tissue phantoms.

Optical coherence tomography (OCT) is an imaging modality capable of acquiring cross-sectional images of tissue using back-reflected light. Conventional OCT images have a resolution of 10-15 microm, and are thus best suited for visualizing tissue layers and structures. OCT images of collagen (with and without endothelial cells) have no resolvable features and may appear to simply show an exponential decrease in intensity with depth. However, examination of these images reveals that they display a characteristic repetitive structure due to speckle. The purpose of this study is to evaluate the application of statistical and spectral texture analysis techniques for differentiating living and non-living tissue phantoms containing various sizes and distributions of scatterers based on speckle content in OCT images. Statistically significant differences between texture parameters and excellent classification rates were obtained when comparing various endothelial cell concentrations ranging from 0 cells/ml to 25 million cells/ml. Statistically significant results and excellent classification rates were also obtained using various sizes of microspheres with concentrations ranging from 0 microspheres/ml to 500 million microspheres/ml. This study has shown that texture analysis of OCT images may be capable of differentiating tissue phantoms containing various sizes and distributions of scatterers.

Algorithms↗
PubMed2001

Addressing and imaging high optical index dielectric ridges in the optical near field.

Experimental observation of light coupling between TiO(2) integrated waveguides of subwavelength cross section and pure three-dimensional evanescent light fields is reported. This near-field optical phenomenon is produced by controlling the location of the focusing of a laser beam totally reflected at the surface of the sample. The phenomenon is observed in direct space with a photon scanning tunneling microscope. Dielectric ridges several tens of micrometers long have been efficiently excited with this technique. Upon excitation, the extremities of the linear dielectric wires display intense light spots localized both inside and around the ridge. For ridge lengths up to 30 microm, the observed phenomenon has been reproduced numerically with a parallel implementation based on the three-dimensional Green dyadic method.

Journal Article↗
PubMed2005

Filtering blebs imaging by optical coherence tomography.

PURPOSE: To assess the ability of standard optical coherence tomography to visualize filtering blebs after glaucoma surgery. METHODS: A prospective interventional case series was conducted in a private practice. Twenty-nine eyes of 24 patients (21 with good, 2 with fair and 6 with poor intraocular pressure [IOP] control) were investigated. After the focus was manually adjusted on the conjunctiva, blebs were scanned perpendicularly to the limbus. RESULTS: Hyporeflective fluid-filled spaces were detected in 19 out of the 21 eyes with good IOP. Within this group, blebs were classified into three different categories according to their optical coherence tomography pattern: type A (featuring a thick wall and a single large fluid-filled space), type B (featuring a thin wall and multiple large fluid-filled spaces) and type C (featuring multiple, irregular and flattened fluid-filled spaces). Fluid-filled spaces were not observed in three out of the six eyes with poor IOP control. Trabeculectomy without antimetabolites was associated with type A blebs (P = 0.015, Fisher's exact test), mitomycin-C trabeculectomy with type B blebs (P = 0.0025) and mitomycin-C phacotrabeculectomy with type C blebs (P = 0.0173). CONCLUSIONS: Although it was not developed to evaluate the anterior segment of the eye, standard optical coherence tomography can visualize filtering blebs and reveal interesting details of their morphology. Clinicians using optical coherence tomography to diagnose glaucoma can take advantage of this ability of the instrument to obtain more information about their patients in the postoperative course of trabeculectomy and phacotrabeculectomy.

Adolescent↗
PubMed2001

New neuroretinal rim blood flow evaluation method combining Heidelberg retina flowmetry and tomography.

AIM: Accurate Heidelberg retina flowmeter (HRF) measurements require correct manual setting of the HRF photodetector sensitivity. The neuroretinal rim produces a weak signal relative to the peripapillary retina. A newly developed HRF alignment and sensitivity protocol, capable of accurate rim measurement, was investigated. METHODS: 18 eyes of nine healthy volunteers were examined by HRF. Three images of each eye were taken using three different imaging methods. Method 1: a conventional image (optic nerve head centred image with photodetector sensitivity optimised for the strong signal from the peripapillary retina); method 2: the setting of method 1 with photodetector sensitivity optimised for the weak signal from the rim; and method 3: the setting of method 2 with the temporal rim margin tangent to the lateral image border to remove the overpowering signal from the temporal peripapillary retina. The neuroretinal rim was defined by the Heidelberg retina tomograph (HRT). Blood flow and reflectivity values (DC component) in the rim area were compared for the three methods using pointwise analysis. Coefficients of variation of repeated measurements in 12 subjects have been calculated for method 3. RESULTS: The neuroretinal rim area measured by method 1 had a significantly lower brightness compared with method 2 and 3 (p=0.0002 and p=0.0002, respectively). Method 2 provided proper sensitivity for the weak signals of the rim area based on rim tissue DC values; however, this sensitivity setting was too high for the strong signal from the peripapillary retina. Method 3 avoided the strong peripapillary signal with the proper signal from the rim and provided significantly higher flow values of the rim area at 75 and 90 percentile pixels (p=0.0065 and p=0.0038 respectively) compared with method 2. Interobserver repeatability ranged from 16.85% to 21.96% for the different parameters. CONCLUSIONS: Method 3 provides an accurate and reproducible flow measurement of the neuroretinal rim area through proper sensitivity for the weak rim signal, alignment, and removal of the strong temporal signal from the image. This new method is recommended to improve accuracy of blood flow measurement in the neuroretinal rim.

Adolescent↗
PubMed2004

Three-dimensional optical tomographic imaging of supersonic jets through inversion of phase data obtained through the transport-of-intensity equation.

We report experimental results of quantitative imaging in supersonic circular jets by using a monochromatic light probe. An expanding cone of light interrogates a three-dimensional volume of a supersonic steady-state flow from a circular jet. The distortion caused to the spherical wave by the presence of the jet is determined through our measuring normal intensity transport. A cone-beam tomographic algorithm is used to invert wave-front distortion to changes in refractive index introduced by the flow. The refractive index is converted into density whose cross sections reveal shock and other characteristics of the flow.

Journal Article↗