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PubMed1999

Drinking water disinfection byproducts: review and approach to toxicity evaluation.

There is widespread potential for human exposure to disinfection byproducts (DBPs) in drinking water because everyone drinks, bathes, cooks, and cleans with water. The need for clean and safe water led the U.S. Congress to pass the Safe Drinking Water Act more than 20 years ago in 1974. In 1976, chloroform, a trihalomethane (THM) and a principal DBP, was shown to be carcinogenic in rodents. This prompted the U.S. Environmental Protection Agency (U.S. EPA) in 1979 to develop a drinking water rule that would provide guidance on the levels of THMs allowed in drinking water. Further concern was raised by epidemiology studies suggesting a weak association between the consumption of chlorinated drinking water and the occurrence of bladder, colon, and rectal cancer. In 1992 the U.S. EPA initiated a negotiated rulemaking to evaluate the need for additional controls for microbial pathogens and DBPs. The goal was to develop an approach that would reduce the level of exposure from disinfectants and DBPs without undermining the control of microbial pathogens. The product of these deliberations was a proposed stage 1 DBP rule. It was agreed that additional information was necessary on how to optimize the use of disinfectants while maintaining control of pathogens before further controls to reduce exposure beyond stage 1 were warranted. In response to this need, the U.S. EPA developed a 5-year research plan to support the development of the longer term rules to control microbial pathogens and DBPs. A considerable body of toxicologic data has been developed on DBPs that occur in the drinking water, but the main emphasis has been on THMs. Given the complexity of the problem and the need for additional data to support the drinking water DBP rules, the U.S. EPA, the National Institute of Environmental Health Sciences, and the U.S. Army are working together to develop a comprehensive biologic and mechanistic DBP database. Selected DBPs will be tested using 2-year toxicity and carcinogenicity studies in standard rodent models; transgenic mouse models and small fish models; in vitro mechanistic and toxicokinetic studies; and reproductive, immunotoxicity, and developmental studies. The goal is to create a toxicity database that reflects a wide range of DBPs resulting from different disinfection practices. This paper describes the approach developed by these agencies to provide the information needed to make scientifically based regulatory decisions.

Acetates↗

PubMed2000

Effects of chlorine, iodine, and quaternary ammonium compound disinfectants on several exotic disease viruses.

The effects of three representative disinfectants, chlorine (sodium hypochlorite), iodine (potassium tetraglicine triiodide), and quaternary ammonium compound (didecyldimethylammonium chloride), on several exotic disease viruses were examined. The viruses used were four enveloped viruses (vesicular stomatitis virus, African swine fever virus, equine viral arteritis virus, and porcine reproductive and respiratory syndrome virus) and two non-enveloped viruses (swine vesicular disease virus (SVDV) and African horse sickness virus (AHSV)). Chlorine was effective against all viruses except SVDV at concentrations of 0.03% to 0.0075%, and a dose response was observed. Iodine was very effective against all viruses at concentrations of 0.015% to 0.0075%, but a dose response was not observed. Quaternary ammonium compound was very effective in low concentration of 0.003% against four enveloped viruses and AHSV, but it was only effective against SVDV with 0.05% NaOH. Electron microscopic observation revealed the probable mechanism of each disinfectant. Chlorine caused complete degeneration of the viral particles and also destroyed the nucleic acid of the viruses. Iodine destroyed mainly the inner components including nucleic acid of the viruses. Quaternary ammonium compound induced detachment of the envelope of the enveloped viruses and formation of micelle in non-enveloped viruses. According to these results, chlorine and iodine disinfectants were quite effective against most of the viruses used at adequately high concentration. The effective concentration of quaternary ammonium compound was the lowest among the disinfectants examined.

African Horse Sickness↗

PubMed2000

[Evaluation of bacterial contamination in disinfectants for domestic use].

OBJECTIVE: To evaluate disinfectants for domestic use for the presence of bacteria, identify them, and determine their tolerance level to benzalkonium chloride. METHODS: Fifty-two samples of commercially available disinfectants for domestic use were acquired at random in the metropolitan area of São Paulo, Brazil, and analyzed to detect the presence of bacterial contaminants. The isolated organisms were identified and their tolerance level to benzalkonium chloride was determined by broth macrodilution method. RESULTS: Sixteen (30.77%) of fifty-two disinfectants sampled were contaminated by Gram-negative bacteria, with counts varying between 10(4) and 10(6) UFC/ml. Alcaligenes xylosoxidans, Burkholderia cepacia and Serratia marcescens were the predominant organisms found. The minimum inhibitory concentration (MIC: mg/ml) of benzalkonium chloride for these bacteria were 2.48, 1.23 and 0.30 to S. marcescens, A. xylosoxidans and B. cepacia, respectively. CONCLUSIONS: The disinfectant formulation containing quaternary ammonium compounds (QACs) may be exposed to contamination by Gram-negative bacteria. The MICs of benzalkonium chloride against the isolated bacteria were low, indicating that the bacteria grown in culture media without QACs lost their tolerance to this biocide.

Disinfectants↗

PubMed2005

Evaluation of the protection conferred by a disinfectant against clinical disease caused by Avibacterium paragallinarum serovars A, B, and C from Argentina.

This work evaluates the efficiency of the administration of the disinfectant N-alkyl dimethyl benzyl ammonium chloride (TIMSEN) in the prevention of the horizontal transmission of serovars A, B, and C of Avibacterium paragallinarum, the causative agent of avian infectious coryza. This disinfectant was administered in drinking water (50 ppm) and once or twice per day by coarse spray (800 ppm, 8 ml per m3 during 3 seconds). In three trials conducted with vaccinated birds, the disinfectant reduced the clinical signs of infectious coryza significantly (P < 0.05). There was no significant effect when the product was used in a fourth trial with unvaccinated birds. Furthermore, the application of only one daily environmental spraying was sufficient to significantly reduce clinical signs. According to these results, in order to diminish the clinical signs of infectious coryza in birds vaccinated against A. paragallinarum, it is recommended to administer this disinfectant in drinking water and by environmental spraying.

Aerosols↗

PubMed1995

Modes of action of disinfectants.

The exact mechanism of action of a disinfectant is not easy to elucidate. The notion of 'target' in the bacterial cell, frequently evoked for the antibiotics, is not clear for disinfectants (except for some, e.g. chlorhexidine). In understanding the mode of action of a disinfectant, it can be difficult to distinguish the primary stage (characteristic of the mode of action) and the secondary stage (consequence of the action). The author describes the actions of disinfectants on the external membrane, cytoplasmic membrane and energy metabolism of cells; these actions include rupture of the membrane, loss of permeability and coagulation of the cytoplasm.

Bacteria↗

PubMed1995

Disinfection in the dairy parlour.

The dairy farmer has the responsibility of producing milk under clean and hygienic conditions, employing appropriate techniques to clean and disinfect the milking equipment and the milking parlour. The ability of raw milk to retain its quality under storage, and the safety of the product for the consumer, can both be directly related to the bacterial content of the milk. In most countries, bacterial content is one of the factors considered in determining the level of payment for milk. Cleaning and disinfection are complementary processes: neither process alone will achieve the desired end result. Milk with low bacterial and somatic cell counts cannot be produced unless milking equipment is effectively cleaned and disinfected between milkings and the cows are kept healthy. The author considers the various sources of bacterial contamination and increased somatic cell counts in raw milk, and describes the cleaning and disinfection practices recommended for the production of milk of good microbiological quality.

Animals↗

PubMed1995

Disinfection in aquaculture.

The authors present information on the susceptibility to various disinfectants of some important pathogens in aquaculture, with special reference to the farming of salmonids. Practical disinfection procedures for use in the aquaculture industry are then described, on the basis of experience obtained in Norway and in other countries with a large salmon-farming industry. In addition to routine disinfection at aquaculture sites, the authors also describe disinfection procedures for use in outbreaks of notifiable diseases, as well as the treatment/disinfection of waste water and offal from the slaughtering and processing of aquaculture products.

Abattoirs↗

PubMed1995

Disinfection of farrowing pens.

The author presents details on the cleaning and disinfection of specialised farrowing accommodation within an intensive pig unit. Procedures are described for use in two quite different sets of circumstances, as follows: in the event of the occurrence of one of the major notifiable epizootic diseases; routine cleaning and disinfection as part of normal management procedures. In the former case, herd slaughter and the cleaning and disinfection of premises are required by law, in accordance with the protocol adopted by international agreement with reference to the specific disease concerned. In the latter case, cleaning and disinfection are not governed by statutes, and the protocol is established to suit the requirements of the individual unit, under the supervision of the farmer and the veterinary adviser.

Animals↗

PubMed1995

Disinfection of food production areas.

Disinfection, other than by heat, is ineffective unless all surfaces have previously been thoroughly cleaned to remove interfering materials. Cleaning is therefore extremely important as part of a two-stage cleaning and disinfection (sanitation) programme. The author describes the principles of sanitation, the chemicals and equipment involved, and the programme of events to be followed. For food products of 'low risk' (in terms of stable shelf life and safety), traditional sanitation programmes are adequate and in some cases disinfection may not be required. However, disinfection is essential for 'high-risk' food products, but this cannot be effectively undertaken without due consideration of hygienic design and possible cross-contamination. To ensure continued satisfactory performance of a sanitation programme, routine assessments should be undertaken.

Animals↗

PubMedDate not supplied

High-performance, low-cost solar collectors for disinfection of contaminated water.

Although the germicidal action of sunlight has long been recognized, its potential for practical applications has to be researched more thoroughly. This paper summarizes the progress made toward a commercially practical collector for solar disinfection applications. Nontracking compound parabolic collectors (CPCs), developed originally for capturing solar photons for thermal energy applications, were examined as potential solar photoreactors. A field demonstration of solar disinfection treatment using commercially manufactured solar reactors was conducted. Field tests showed successful destruction of Escherichia coli and Enterococcus faecalis and have provided data for full-scale design of water treatment systems. From above observations, a throughput value of 50 L/m2 h for the low-cost CPC reactor tested was estimated. For a 190 m3/d (0.05 MGD) facility, the estimated total costs for disinfection using UV-A is U.S. $0.19/m3 ($0.70/1000 gal). The use of near-UV sunlight to disinfect water supplies seems promising in rural communities of developing countries where treated water is unavailable.

Bacillus subtilis↗

PubMed2005

Efficacy of an iodophore teat disinfectant against Staphylococcus aureus and Streptococcus agalactiae in experimental challenge.

A 1.0% iodophore teat disinfectant (Full-Bac) was evaluated in comparison with a positive control (Bovadine), a commercially available 1.0% iodophore teat disinfectant. The study was conducted under conditions of experimental challenge, following the guidelines recommended by the National Mastitis Council. The test product and a positive control were compared in 41 cows, with 82 teats receiving each product at milking, during a 10-wk study period. There were no differences between the test product and the positive control in new intramammary infections due to Staphylococcus aureus, which averaged 13.4% in each of the 2 treatment groups. Additionally, no statistical difference was seen between the test product and positive control in new intramammary infections by Streptococcus agalactiae, which averaged 8.5 and 6.1% for the Full-Bac and Bovadine groups, respectively. Teat skin and teat end condition scores were statistically evaluated at wk 1, 5, and 9 of the study, and no significant differences were observed between the treatment groups. The test teat disinfectant provided similar germicidal activity to that of the positive control teat disinfectant with no adverse effects on teat skin or teat end condition during the warm-season study period.

Animals↗

PubMed2005

Disinfection of dairy and animal farm wastewater with radiofrequency power.

Radiofrequency (RF) power was investigated as a new, physical (nonchemical), thermal process to disinfect wastewater from dairy and animal facilities. Samples (n = 38) from 8 dairy, 2 calf, and 3 swine facilities in California were collected over a 3-yr period and characterized for their dielectric properties, chemical composition, and suitability for thermal processing using RF power. To establish efficacy for disinfection, selected samples were inoculated with high levels (10(6) to 10(9) cfu/mL) of bacterial pathogens such as Salmonella sp., Escherichia coli O157:H7, and Mycobacterium avium ssp. paratuberculosis and processed with an RF prototype system. The capabilities of RF power as a method for thermal disinfection of wastewater were demonstrated when bacteria pathogens were completely and rapidly (<1 min) inactivated when temperatures of 60 to 65 degrees C were achieved. Furthermore, RF technology can be used for large-scale, batch or continuous and portable applications, allowing significant improvements in energy-use efficiencies compared with conventional thermal (surface heating) technologies. Therefore, RF power has potential as an alternative to disinfect dairy/animal farm wastewater before recycling.

Agriculture↗

PubMed2004

Safety and protective effect of a disinfectant (STEL water) for white spot syndrome viral infection in shrimp.

The efficacy of STEL water for protection against white spot syndrome virus (WSSV) infection was evaluated using shrimp. The LC50 of residual chlorine (Cl-) in STEL water for brood-stock and 2-mo-old shrimp were 2.3 and 3.2 ppm, respectively. All 2-month-old shrimp raised in seawater containing more than 40 microl 2l(-1) of a WSSV-infected tissue homogenate died within 3 d post-exposure (dpe). Thus, a 10-fold dose of 400 microl 2 l(-1) was used in the disinfection tests. Low concentrations of STEL water effectively prevented mortality of shrimp at this challenge dose. All 2-month-old shrimp exposed to seawater with 400 microl of viral homogenate disinfected with STEL water at Cl- concentrations over 0.125 ppm for 1 and 10 min, lived until 5 dpe. With 5-mo-old shrimp, all positive control shrimps died within 3 dpe, whereas most shrimp reared in seawater disinfected with STEL water for 1 h before addition of homogenate lived until 5 dpe. Results suggested that continuous disinfection of seawater with STEL water may be effective for preventing WSSV infection in shrimp.

Animals↗

PubMed1985

Evaluation of four disinfectants under poultry grow-out conditions using contact agar sampling technique.

Four commercial disinfectants used in poultry house sanitation procedures were evaluated for efficacy using a specialized petri dish (Rodac plate) for contact sampling. The samples were taken from a variety of surface materials commonly found in poultry rearing facilities. Two media were used, one for bacteria and one for mold. Predisinfection samples were taken following removal of chickens and litter. Postdisinfection samples were taken 4 hr after room treatment with a high pressure spray apparatus. The most effective disinfectant for reducing bacteria colony counts was a product that contained glutaraldehyde. The other products, ranking in order of efficacy for bacteria, contained cresylic acid, iodophors, and a combination of quaternary ammonium compound and formaldehyde. The most effective disinfectant for reducing mold colony counts was the product containing cresylic acid. The other products, ranking in order of efficacy for mold, contained iodophors, the combination of quaternary ammonium compound and formaldehyde, and glutaraldehyde. The most effective overall disinfectant was the cresylic acid product.

Animals↗

PubMed1987

Efficacies of mixtures of disinfectants and insecticides.

Efficacies of mixtures of diluted commercial formulations of selected insecticides and disinfectants were evaluated. Insecticides tested included representative pyrethroids (fenvalerate [Ectrin WDL and WD] and permethrin [Ectiban EC]), organophosphates (dichlorvos [Vapona EC], tetrachlorvinphos [Rabon WP] and dichlorvos/tetrachlorvinphos [RaVap EC], and a carbamate (carbaryl [Sevin S]). Disinfectants tested included representatives of cresylic acid (Biolene), cresylic acid/phenol (BioGuard X-185), phenol (1-Stroke Environ), quaternary ammonium (BioGuard S-3 and PFP-4), quaternary ammonium/formalin (DC & R), and formalin classes of disinfectants. Mixtures were tested for toxicity to two target insects (Musca domestica on plywood, Alphitobius diaperinus in litter) and two bacteria (Pseudomonas aeruginosa and Staphylococcus aureus). Of 56 mixtures evaluated, 24 showed reduced insecticidal toxicity and 35 showed reduced bactericidal activity compared with insecticides or disinfectants alone.

Animal Husbandry↗

PubMed2000

Microbial testing methods for detection of residual cleaning agents and disinfectants-prevention of ATP bioluminescence measurement errors in the food industry.

The ATP luminescence measurement is based on the presence of an enzymatic reaction and may significantly be affected by cleaning agents and disinfectants. In addition, disinfectants can also reduce the activity of the luciferase enzyme and also act as ATP-releasing agents. The agents disrupt the cell walls but preserve ATP in measurable form, and therefore correlation with culture methods can be poor. Therefore, if a rapid method is used to detect ATP, a control must be used for reliable results. The possible effect of disinfectants can be eliminated with a rapid test to minimize sources of error. In the present study a microbiological residue testing method that is nonspecific for residues was developed. The effects of a total of 38 commercial cleaning agents and disinfectants of various types were assessed using two microbiological methods, the Vibrio fischeri photobacteria test and Micrococcus luteus inhibition zone technique. The results show that the V. fischeri photobacteria test is very sensitive. This test can therefore be used for testing cleaning agent residues on surfaces in very small amounts. A small study was also carried out in a food factory to show applicability in processing facilities. The study showed, that a need for this type of method exists in food processing.

Adenosine Triphosphate↗

PubMed2003

Selection of fluorescent probes for flow cytometric viability assessment of Listeria monocytogenes exposed to membrane-active and oxidizing disinfectants.

The aim of this study was to select fluorescence methods for use as alternatives to plate counting to assess the viability of Listeria monocytogenes cells exposed to benzalkonium chloride (BAC) and hydrogen peroxide, two disinfectants with different mechanisms of action. A further aim of this study was to determine whether growth phase influences fluorescence labeling and whether it is possible to predict whether a probe will be a good viability indicator for cells exposed to a certain disinfectant on the basis of the mechanism of action of the disinfectant and the target of the fluorescent probe. The fluorescence methods used were labeling with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC; dehydrogenase activity), labeling with TOTO-1 iodide (TOTO; membrane-impermeant probe), and assessment of pH gradient maintenance in a low-pH buffer after labeling with the pH-sensitive probe 5-(and 6)-carboxyfluorescein succinimidyl ester (CFSE) (the pH(in) method). Growth phase influenced fluorescent labeling. However, the cutoff value for distinction between viable and nonviable cells was the same for both growth phases. The viability (determined by plate counts) of BAC-exposed cells correlated well with CTC labeling and TOTO exclusion. For both BAC-exposed and hydrogen peroxide-exposed cells, the pH(in) method gave a good qualitative indication of viability, sublethal damage, and cell death. CTC labeling and TOTO exclusion did not correlate with the viability of hydrogen peroxide-exposed cells. Our results demonstrate that even if the mechanism of action of a disinfectant is known, in some cases it is still difficult to predict whether a certain fluorescent probe is suitable for viability assessment. Thus, the proper selection of fluorescent probes for the assessment of the efficacy of antimicrobial agents is essential.

Benzalkonium Compounds↗

PubMed2006

Development of pulsed UV light processes for surface fungal disinfection of fresh fruits.

Pulsed UV (PUV) power techniques were studied as a nonthermal, residue-free alternative to contact pesticides and to evaluate the surface disinfection of fresh fruits using this type of extremely rapid, high-peak power UV beams. Coherent 248-nm beams from excimer lasers were used to simulate a variety of pulsed light sources now commercially available. Surface disinfection on a series of fresh fruits (i.e., apples, kiwi, lemon, nectarines, oranges, peaches, pears, raspberries, and grapes), representing economically important commodities, were studied and evaluated. Plant (fungal) pathogens were rapidly (<10 s), efficiently (>5 log), and reproducibly killed on fruit surfaces. However, in naturally infected or inoculated (sprayed) fruits, a fraction of the inoculum may penetrate into the epidermis or locate in injured tissue in crevices or in surface irregularities. Under these conditions, only partial disinfection was obtained due to UV shielding (shadowing) effects, which prevent the highly directional, coherent PUV beam from reaching its target. For maximum disinfection efficiency, coherent PUV sources must be combined with dispersing reflectors, and fruits must be handled to ensure uniform exposure to multidirectional incident beams. New, existing, noncoherent, broadband, pulsed light beams (high in UV emission) from arc lamps appear to provide adequate PUV light sources capable of meeting the conditions for commercial applications in slight-modified conveyorized operations. Therefore, PUV techniques may provide effective, commercial-scale, reliable, and viable residue-free alternatives to chemical (contact) pesticides.

Colony Count, Microbial↗