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Filarid parasites of South Kalimantan (Borneo) Indonesia. Wuchereria kalimantani sp. n. (Nematoda: Filarioidea) from the silvered leaf monkey, Presbytis cristatus Eschscholtz 1921.

On the basis of morphological characteristics of adult and microfilarial stages, Wuchereria kalimantani sp. n. (Nematoda: Filarioidea) is described. The parasites were recovered from the inguinal lymph nodes and testes of silvered leaf monkeys (Presbytis cristatus) from a rubber estate, Banjar Regency, South Kalimantan (Borneo), Indonesia. Adult males are characterized by having more than 24 caudal papillae, a left spicule with a simple lamina, four pairs of sessile caudal papillae, a blunt tail, a mean overall length of 41 mm, and sheathed microfilariae with a nude tail tip; accordingly, the parasite is placed in the genus Wuchereria. This is the second species of Wuchereria described on the basis of both adult and embryonic forms, and is the first such species that has been found to infect primates naturally.

Animals↗

An epizootic of measles in captive silvered leaf-monkeys (Presbytis cristatus) in Malaysia.

An epizootic of measles occurred in a group of 31 silvered leaf-monkeys (Presbytis cristatus) that had been in captivity for 4-12 months. Twenty-four of the monkeys exhibited a maculopapular rash that persisted for 6-9 days. A serous to mucopurulent nasal discharge and conjunctivitis were seen in some animals. Eight monkeys died during the epizootic; however, their deaths could not be directly attributed to measles. Serum samples from the surviving monkeys collected 1-2 months prior to, and 5 weeks after, the epizootic were examined by the complement-fixation and hemagglutination-inhibition tests for antibodies to measles virus. The preepizootic complement-fixation titers were all less than 1:4 and hemagglutination-inhibition titers, less than 1:10. The postepizootic complement-fixation titers in 21 of 23 surviving monkeys ranged from 1:8 to 1:128, and hemagglutination-inhibition titers in 22 of 23 monkeys ranged from 1:40 to 1:80 or greater.

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On palmar and plantar pattern variability of the two baboon genera Papio and Theropithecus.

In general the results of the investigators Biegert (1961) and Gladkova (1958), concerning the palm and sole skin patterns in baboons can be confirmed, and some features can serve to distinguish some of the species in a very characteristic manner. The findings of our investigations of the papillary system substantiate the proposed taxonomic classification for baboons by Buettner-Janusch in 1966. The value and significance of the inter- and intra-species differences in baboons, with regard to morphological and quantified pattern analysis, could be fully evaluated when further comparisons of the presented data are accomplished and compared with those of other primate species and families.

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[Phylogeny of lice (Insecta: Anoplura) of the Old World Monkey (Catarrhina)].

The genera Pediculus and Pthirus were studied cladistically, although the genus Pedicinus was also taken into account. Morphological characters from the literature, and some established through direct study were analyzed. Using five methods of cladistic analysis, one most parsimonious tree with a c.i. = 0.84 and a length of 38 was obtained ((Pedicinus)+(Paenipediculus+(Parapediculus+(Pedicu lus humanus capitis+Pediculus humanus humanus). A novelty of this study is the inclusion of the subgenus.

Animals↗

Chemotherapeutic effect of CGI 18041 against subperiodic Brugia malayi infection in Presbytis cristata.

CGI 18041, an adduct of benzothiazol isothiocyanate N-methyl piperazine, was evaluated for its antifilarial properties in subperiodic Brugia malayi infected Presbytis cristata. Animals experimentally infected with 200-400 subperiodic Brugia malayi infective larvae, were matched according to microfilaria density, infective dose, and duration of infection. They were then randomly assigned to various treatment and control groups. The compound was suspended in 1% Tween 20 in distilled water, sonicated, and then fed to monkeys using a stomach tube. Control animals received an equivalent volume of drug diluent. CGI 18041 at a single oral dose of 50 mg/kg had complete adulticidal and microfilaricidal activities against subperiodic B. malayi in P. cristata. It was also extremely effective at a single dose of 25 mg/kg, the final geometric mean microfilaria count being 1.6% of initial level, and only 1.0% of the infective dose was recovered as live adult worms at autopsy 6 weeks post-treatment. In control animals, these were 226.9% and 5.56% respectively.

Administration, Oral↗

Innervation of the ligament of the femoral head in the formosan rock monkey: a retrograde HRP tracing and ultrastructural study.

The innervation of the ligament attached to the femoral head of the Formosan rock monkey (Macaca cyclopis, Swinhoe) was investigated by means of the retrograde HRP tracing method and by electron microscopy. WGA/HRP was injected into the ligament of the left femoral head. Numerous neurons labelled with HRP were found in the ipsilateral lumbar sympathetic ganglia, from L1 to L7, with a peak in the L3 to L5 region. Labelled neurons were also found in the ipsilateral spinal dorsal root ganglia, from L1 to L7 and concentrated at L4. Ultrastructurally, the nerve fascicles to the ligament had an average diameter at 32.8 microns. They contained both myelinated and unmyelinated axons and accompanied the blood vessels. Sensory receptors were not found in the ligamentum proprium but free nerve endings were observed in the walls of the blood vessels. The present results indicate that the nerve fibres to the ligament of the femoral head may be involved in regulating blood vessel calibre in addition to having a sensory function.

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Transmission of human spongiform encephalopathies to experimental animals: comparison of the chimpanzee and squirrel monkey.

The agents of kuru and Creutzfeldt-Jakob disease have been consistently transmitted from patients with those diseases to chimpanzees and squirrel monkeys, as well as to other new-world primates, with average incubation periods of two or three years. No other animals have been found so consistently susceptible to the agents in human tissues. More rapid and convenient assays for the infectious agents would greatly facilitate research on the spongiform encephalopathies of humans.

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Presbytis entellus, a new experimental host for Plasmodium fragile.

The course of blood-induced Plasmodium fragile infection in 3 simian hosts, Macaca radiata (bonnet monkey), Macaca mulatta (rhesus monkey), and Presbytis entellus (Indian langur), was studied. The infection in 8 bonnet monkeys was very mild and nonfatal. In M. mulatta, P. fragile produced acute initial infection followed by anemia and death in 3 of 6 monkeys. The parasite has a relatively high level of virulence for P. entellus, a new experimental host for P. fragile, in which peak parasitemia ranging between 15 and 75% was recorded, and the infection was uniformly fatal. Plasmodium fragile infection in P. entellus can serve as an ideal biological counterpart to human Plasmodium falciparum.

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Tuberculosis in imported nonhuman primates--United States, June 1990-May 1993.

Nonhuman primates (NHPs) shipped to the United States must be quarantined and monitored for evidence of infectious diseases transmissible to humans. During May-October 1992, CDC investigated reports from NHP importers of mycobacterial infections in NHPs recently imported from Mauritius. This report describes a review of tuberculosis (TB) in all 249 imported shipments of CDC-permitted NHP species in the United States from June 1990 through May 1993 and updates recommendations regarding the identification and control of TB in imported NHPs and in workers exposed to such animals.

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Parallel pathways in the retina of Old and New World primates.

Old-world simians are all trichromats, but in most new-world primates there is a polymorphism; males are dichromats but most females are trichromats. In the old world simian, luminance and red-green chromatic channels defined by psychophysical experiments have as a basis parasol ganglion cells of the magnocellular (MC) pathway and midget ganglion cells of the parvocellular (PC) pathway respectively. Small bistratified ganglion cells provide a basis for a blue-yellow chromatic channel, which should probably be considered a separate entity. In both dichromatic and trichromatic new-world animals, the MC pathway and the small bistratified, blue-yellow system seem anatomically and physiologically similar to those in their old-world relatives. The midget ganglion cells of the parvocellular pathway in trichromats are anatomically and physiologically similar to the old-world pattern. In dichromatic animals, they are anatomically similar and physiologically resemble those of trichromatic animals, except for the lack of chromatic opponency. We conclude that these three systems may from a basic pattern for the visual pathway of primates. However, the results from dichromats indicate that the evolution of trichromacy may be found to be more complex than presently supposed.

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The nasopalatine duct and the nasal floor cartilages in catarrhine primates.

The nasal floor structures in catarrhine primates have been studied on the basis of histological serial sections of older fetuses of Presbytis, Hylobates, Gorilla, Pan, Pongo and Homo. For outgroup comparisons, sections of the strepsirhine Daubentonia and the platyrrhine Callimico are presented; in these taxa the nasal floor cartilages are intimately connected not only with the nasopalatine duct (STENO) but also with the persisting vomeronasal organ (JACOBSON). Whereas the vomeronasal organ has disappeared as a functioning sensory organ in catarrhines (occasional embryonic vestiges are reported), the nasopalatine duct is retained in cercopithecoids, hylobatids and in Gorilla; in Pan, Pongo and Homo at least remnants of the duct and of the associated cartilages are preserved. The systematic meanings of these findings have been discussed.

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Plasmodium inui is not closely related to other quartan Plasmodium species.

Plasmodium inui (Halberstaedter and von Prowazek, 1907), a malarial parasite of Old World monkeys that occurs in isolated pockets throughout the Celebes, Indonesia, Malaysia, and the Philippines, has traditionally been considered to be related more closely to Plasmodium malariae of humans (and its primate counterpart Plasmodium brasilianum), than to other primate Plasmodium species. This inference was made in part because of the similarities in the periodicities or duration of the asexual cycle in the blood, the extended sporogonic cycle, and the longer period of time for development of the pre-erythrocytic stages in the liver. Both P. inui and P. malariae have quartan (72 hr) periodicities associated with their asexual cycle, whereas other primate malarias, such as Plasmodium fragile and Plasmodium cynomolgi, are associated with tertian periodicities (48 hr), and Plasmodiumn knowlesi, with a quotidian (24 hr) periodicity. Phylogenetic analyses of portions of orthologous small subunit ribosomal genes reveal that P. inui is actually more closely related to the Plasmodium species of the "vivax-type" lineage than to P. malariae. Ribosomal sequence analysis of many different, geographically isolated, antigenically distinct P. inui isolates reveals that the isolates are nearly identical in sequence and thus members of the same species.

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