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Prognostic significance of elevated endothelin-1 levels in patients with colorectal cancer.

BACKGROUND: Prognostic factors from clinical, laboratory and pathological data of patients with colorectal cancer are essential to identify high-risk groups to whom beneficial adjuvant therapy could be given. Endothelin-1, a growth factor, has been associated with the development and spread of solid tumours. This prospective study was performed to determine whether preoperative plasma big ET-1 levels might be useful as a prognostic indicator in patients with colorectal carcinoma. METHOD: Sixty-five consecutive patients with colorectal cancer confirmed by biopsy were included prospectively into this study over a 12-month period. Plasma samples from a peripheral vein were obtained prior to surgery. Univariate analysis of survival using age (< or > 70 years), sex, Dukes' stage (A&B versus C), tumour size (< or > 50 mm), vascular invasion and plasma big ET-1 levels was performed and significant factors were then analysed with the Cox regression model. RESULTS: Three variables, age, Dukes' tumour stage and plasma big ET-1 levels, were found to have prognostic significance (p<0.05). Factors associated with a poorer prognosis were age >70 years (p=0.02), Dukes' C tumours (p=0.04) and plasma big ET-1 levels >4.2 pg/mL (p=0.02). The Cox regression model identified the same three variables as having independent prognostic value for overall survival. CONCLUSION: Preoperative plasma big ET-1 levels may be useful in predicting overall survival in patients with colorectal cancer. Plasma big ET-1 levels may be useful in the selection of high-risk lymph node-negative patients with colorectal cancer for adjuvant therapy.

Aged↗

Porosity of resin filling materials.

Four paste-paste types of resin filling materials and three capsulated products were tested with respect to porosity content and size distribution of the pores. The data were obtained by using an automatic image analyzer. The paste-paste products were inserted into the test cavities by a bulk-packing technique and an injection technique. The results showed that the use of a syringe reduced the porosity content, mainly by the elimination of big pores. The capsulated products were not less porous than the paste-paste materials.

Dental Materials↗

[Percutaneous therapy of painful arthritis].

Pain in osteoarthrosis of the big weight bearing joints is either derived from periarticular ligaments, tendons, fasciae, muscles, bursae--peri-arthropathy as sign of decompensation--or from the reactive synovitis with or without effusion. NSAIDs systemically administered have been so far considered as first choice medication together with physical therapy. New pharmacokinetic data on the topical, percutaneous application of NSAIDs (ibuprofen, diclofenac, indomethacin, some salicylates and to a lesser degree for etofenamate and piroxicam) have demonstrated relevant advantages of the percutaneous route over the systemic one in soft tissue rheumatism. NSAIDs, mentioned above, locally administered as cream, gel or spray, quickly penetrate through the corneal layer of the skin at the site of application, reach high effective concentrations in subcutis, fasciae, tendons, ligaments and muscles, lesser in joint-capsule and -fluid indicating direct penetration. The blood levels of topical NSAIDs are extremely low with no systemic side effects, especially no gastric toxicity; however, local skin irritation is observed (1 to 2%). In contrast to this, systemic (oral) NSAIDs lead primarily via high blood levels to a much lesser concentration--only one tenth--in particular soft tissues with a high incidence of side effects. In conclusion the percutaneous application of certain NSAIDs has become a well established therapeutic regimen in painful osteoarthrosis and in all other inflammatory degenerative and posttraumatic alterations of soft tissue structures.

Administration, Topical↗

Is remaking the hospital making money?

Touted as a way to shore up margins and help stabilize hospital finances, reengineering caught hold in a big way. A study released exclusively to H&HN shows hospitals that fared well after reengineering fared really well, while those that performed poorly beforehand did just as poorly afterward.

Data Collection↗

[Scarf osteotomy for the treatment of forefoot deformity].

PURPOSE OF THE STUDY: The outcome of surgical treatment in hallux valgus is sometimes unsatisfactory for both the patient and the surgeon. The valgus position of the big toe in the metatarsophalangeal joint is associated with a deviation to varus of the first metatarsal, resulting in the space between the first and second metatarsals called the intermetatarsal (IMT) angle. In most patients the angle is between 10 and 20 degrees. These patients were indicated for scarf osteotomy as this method has been reported to achieve good outcomes. The results are compared with the relevant literature data on foot osteotomy. MATERIAL: Our group involved 62 scarf osteotomies carried out on 49 patients who were followed up for an average of 18 months (range, 6-36 months). Three patients underwent surgery on both feet in one stage, five had bilateral surgery in two stages. The average pre-operative IMT angle was 16 degrees (range, 9-21 degrees) and the average hallux valgus angle was 37 degrees. METHODS: The patients were indicated for surgery on the basis of subjective complains and weight-bearing radiographs. Scarf osteotomy was performed by the Barouk technique. From a signle incision in the first intermetatarsal space, the lateral articular capsule was released, adductor tendon was dissected and sesamoid bones were reduced. The first metatarsal was exposed from an incision along its medial axis, the bunion was excised and Z-osteotomy of the metatarsal was performed. The distal fragment was shifted laterally, fixed with two 3.5 mm Poldi screws, and the capsule was closed under tension with transosseal suture. If necessary, an additional procedure on the big toe phalanges or osteotomy of the other metatarsals are carried out. From the second post-operative day the patients were allowed to walk on the heel, after removal of sutures they walked wearing a special sandal and, from the third week onwards, full weight-bearing was allowed. The average hospital stay lasted 4 days. The evaluation of post-operative results was based on radiograms, subjective feelings of the patients and clinical assessment of the range of big toe motion. RESULTS: Out of 62 operations carried out on 49 patients (average age, 41.5 years), 23 were performed on the right and 23 on the left foot; bilateral surgery was carried out in three patients in one stage and in five patients in two stages. Simultaneously, the Weil osteotomy was performed on six feet, Akin osteotomy of the big toe phalanges on five feet, Braggard surgery of the second toe on three feet, and scarf osteotomy of the fifth metatarsal on three feet. All feet were indicated for scarf osteotomy because of pain and, in 56 feet, also esthetic reasons were involved. The patients' subjective post-operative assessments were as follows: satisfaction with the outcome in 58 feet, pain associated with tight shoes in two feet, pain while walking in six feet, and dissatisfaction with the big toe shape in one patient.The average IMT angle of 16 degrees and hallux valgus angle of 37 degrees on the pre-operative radiograms showed improvements to 9 degrees and 18 degrees, respectively, on the post-operative X-ray. The sesamoid bones were reduced in all cases. After surgery the average range of motion was restricted as follows: plantar flexion by 7 degrees (to 23 degrees) and dorsal flexion by 6 degrees (to 54 degrees). The complications included one fracture of the head requiring osteosynthesis, one failure of fixation with repeat valgus osteotomy, three cases of insufficient correction of a valgus position that had to be treated by additional osteotomy of the first toe phalanges. DISCUSSION: Out of other types of osteotomy (Funk, Dega, spike osteotomy), outcomes similar to scarf osteotomy have been achieved only by the Austin procedure. However, in this, shifting of the distal fragment is limited and the results show that the Austin method should be preferred in deformities with an IMT angle of about 10 degrees. Scarf osteotomy in addition allows for early weight-bearing, does not produce shortening of the first metatarsal but permits its elongation and elevation by oblique osteotomy, if necessary. It can also be used for the fifth metatarsal. The drawbacks include a more complicated surgical technique and higher risk of complications; shifting of the distal fragment is also limited and, for this reason, scarf osteotomy is not effective in deformities with an IMT angle higher than 20 degrees. CONCLUSIONS: Scarf osteotomy is an effective procedure for a moderate valgus deformity of the big toe with an IMT angle between 10 and 20 degrees. It permits early weight-bearing of the treated extremity. It requires exact pre-operative planning and strict adherence to the operative technique.

Adult↗

Who's afraid of the big bad Wolff?: "Wolff's law" and bone functional adaptation.

"Wolff's law" is a concept that has sometimes been misrepresented, and frequently misunderstood, in the anthropological literature. Although it was originally formulated in a strict mathematical sense that has since been discredited, the more general concept of "bone functional adaptation" to mechanical loading (a designation that should probably replace "Wolff's law") is supported by much experimental and observational data. Objections raised to earlier studies of bone functional adaptation have largely been addressed by more recent and better-controlled studies. While the bone morphological response to mechanical strains is reduced in adults relative to juveniles, claims that adult morphology reflects only juvenile loadings are greatly exaggerated. Similarly, while there are important genetic influences on bone development and on the nature of bone's response to mechanical loading, variations in loadings themselves are equally if not more important in determining variations in morphology, especially in comparisons between closely related individuals or species. The correspondence between bone strain patterns and bone structure is variable, depending on skeletal location and the general mechanical environment (e.g., distal vs. proximal limb elements, cursorial vs. noncursorial animals), so that mechanical/behavioral inferences based on structure alone should be limited to corresponding skeletal regions and animals with similar basic mechanical designs. Within such comparisons, traditional geometric parameters (such as second moments of area and section moduli) still give the best available estimates of in vivo mechanical competence. Thus, when employed with appropriate caution, these features may be used to reconstruct mechanical loadings and behavioral differences within and between past populations.

Adaptation, Physiological↗

Characterization of immunoreactive dynorphin B and beta-endorphin in human plasma.

Dynorphins and beta-endorphin in human plasma were characterized and studied quantitatively using radioimmunoassay, high-performance liquid chromatography (HPLC), and mass spectrometry. Most immunoreactive (ir) dynorphin B and beta-endorphin in human plasma coeluted with authentic peptides in analysis. Dynorphin A was not detected. Added to human plasma it was rapidly converted into Leu-enkephalin-Arg6 followed by elimination of the C-terminal arginine after prolonged incubation. The rate of dynorphin A conversion was estimated at 40 pmol/min/microl plasma. This process was inhibited by the thiol protease inhibitor, PHMB and by EDTA. Dynorphin B, alpha-neoendorphin and big dynorphin were virtually not metabolized by plasma proteases under the same conditions. beta-endorphin was processed into beta-endorphin(1-19) and the corresponding C-terminal counterpart beta-endorphin(20-31) at a rate of about 25 pmol/min/microl of plasma. Based on the above data, a reliable strategy was established to measure dynorphin B- and beta-endorphin-ir in human plasma samples. The basal levels in a male control group were 0.99 +/- 0.11 (n = 11) and 16.3 +/- 1.5 (n = 11) fmol/ml plasma, respectively.

Adult↗

Prognosis of colorectal cancer patients with elevated endothelin-1 concentrations.

AIM: Prognostic indicators from clinical, laboratory and pathological data of patients with colorectal cancer are essential to identify high-risk groups in whom adjuvant therapy could be beneficial. Endothelin-1 (ET-1), a growth factor, has been associated with the development and spread fo solid tumours. This prospective study was performed to determine whiter preoperative plasma big ET-1 concentrations might be useful as a prognostic indicator in patients with colorectal carcinoma. METHODS: Overall, 65 consecutive patients with colorectal cancer confirmed by biopsy were include prospectively in this study from 1998 to 2001. Plasma samples from a peripheral vein were obtained prior to surgery. Univariant analysis of survival used age (less than or more than 70 years), gender, Dukes' stage (A/B vs C), tumour size (less than or more than 50 mm), vascular invasion, and plasma big ET-1 concentrations, and significant factors were then analysed using a Cox regression model. RESULTS: Three variables, age, Dukes' tumour stage and plasma big ET-1 concentration, and prognostic significance (p < 0.05). Factors associated with a poorer prognosis were age more than 70 years (p = 0.02), Dukes' C (p = 0.04) and plasma big ET-1 concentration more than 4.2 pg/mL (p = 0.02). The Cox regression model identified the same three variables as having independent prognostic value for overall survival. CONCLUSION: Preoperative plasma big ET-1 concentration may be useful in predicting overall survival in patients with colorectal cancer. Plasma big ET-1 concentrations may be useful in the selection of high-risk, lymph node-negative patients with colorectal cancer for adjuvant therapy.

Adult↗

Phylogeography of the tidewater goby, Eucyclogobius newberryi (Teleostei, Gobiidae), in coastal California.

The tidewater goby, Eucyclogobius newberryi, inhabits discrete, seasonally closed estuaries and lagoons along approximately 1500 km of California coastline. This species is euryhaline but has no explicit marine stage, yet population extirpation and recolonization data suggest tidewater gobies disperse intermittently via the sea. Analyses of mitochondrial control region and cytochrome b sequences demonstrate a deep evolutionary bifurcation in the vicinity of Los Angeles that separates southern California populations from all more northerly populations. Shallower phylogeographic breaks, in the vicinities of Seacliff, Point Buchon, Big Sur, and Point Arena segregate the northerly populations into five groups in three geographic clusters: the Point Conception and Ventura groups between Los Angeles and Point Buchon, a lone Estero Bay group from central California, and San Francisco and Cape Mendocino groups from northern California. The phylogenetic relationships between and patterns of molecular diversity within the six groups are consistent with repeated, and sometimes rapid, northward and southward range expansions out of central California caused by Quaternary climate change. Plio-Pleistocene tectonism, Quaternary coastal geography and hydrography, and historical human activities probably also influenced the modern geographic and genetic structure of E. newberryi. The phylogeography of E. newberryi is concordant with phylogeographic patterns in several other coastal California taxa, suggesting common extrinsic factors have had similar effects on different species. However, there is no evidence of a phylogeographic break coincident with a biogeographic boundary at Point Conception.

Animals↗

Restoring biodiversity in the Gwydir Wetlands through environmental flows.

As part of the Water Reforms process, environmental flow rules have been progressively implemented in New South Wales rivers. The Integrated Monitoring of Environmental Flows (IMEF) is a major project established to better understand how rivers and associated wetlands respond to environmental water allocations. The results presented here represent the vegetation data collected for the testing of the hypothesis that "protecting or restoring a portion of freshes and high flows and otherwise maintaining natural flow variability will replenish anabranches and riverine wetlands, restoring their biodiversity". The study site is the Ramsar listed Gwydir Wetlands, located on the Gingham and Gwydir (Big Leather) Watercourses in the Lower Gwydir Valley, 100 km west of Moree. The expansion of irrigated agriculture in the lower Gwydir valley has severely altered flow regimes in the wetlands. The spread of the weed Phyla canescens (Lippia) is of major concern to landholders in the Gwydir Wetlands. Results indicate that Paspalum distichum (Water couch) and Eleocharis plana (Ribbed spike-rush) can maintain dominance over Phyla canescens if flooding occurs on a semi-regular basis. Conversely, Eichhornia crassipes (Water hyacinth) is a rampant noxious weed of open water in the Gwydir Wetlands, and has quickly spread in areas that are inundated for long periods. Management of this weed requires periodic drying of the wetlands to cause desiccation and death of the plants. The flooding requirement of individual species and plant associations in the Gwydir Wetlands are currently not fully understood. By providing better information on the consequence of different flows, the IMEF project will help to develop better management strategies to shift the dominance from introduced species such as P. canescens and E. crassipes to more desirable native plant species.

Conservation of Natural Resources↗

[Ultrastructural changes in the cells of the vasopressin-sensitive epithelia during the stimulation of water transport].

The author's own and literary data are reviewed on the ultrastructural changes in cells of tight epithelia at the stimulation of osmotic water flows by vasopressin. The results of the relevant morphofunctional studies proved the idea of the transcellular water transport, being the following: 1) the absence of structural changes of tight junction under big water flows; 2) the appearance of domains with high water permeability (aggregates of IMP) in the apical membranes of cells transporting water; 3) changes in the vacuolar system of cells resulting in the appearance of gigantic vacuoles involved in transcellular water transport and in osmoregulation; 4) the redistribution of microfilaments and the enlargement of microtubules during the water transport. Hypotheses are discussed accounting for the origin of water channels from intracellular sources (aggrephores or/and granules); in addition, structural peculiarities and genesis of gigantic vacuoles are considered.

Animals↗

[Localization of impacted maxillary canines by palpation and orthopantomography].

In orthodontic practice occurred problems associated with labio-palatal localization of impacted permanent maxillary canines. Not always is possible to determinate labio-palatal location of these teeth on the basis of palpation, therefore clinical examination aided by radiographic investigation. Orthopantomogram is the most widely used radiograph in orthodontics. The purpose of present study-test diagnostic value of palpation and orthopantomography in cases of maxillary canines impaction on the basis of evaluation of migration and inclination of impacted teeth. Thirty nine patients of Clinic of Orthodontics, Kaunas University of Medicine, orthopantomograms were available for analysis. Forty two impacted canines were investigated. Only 64% of canines could be palpated. It depended on the vertical position of the tooth. Distribution of the values of canine inclination to the middle of upper jaw was too big for more precise statistical analysis. Canines, which were medial to the 3th horizontal sector (these canines overlapped longitudinal axis of the lateral incisor), were located palatally.

Adolescent↗

State Medicaid officials battle drug firms in war to cut costs.

As state Medicaid agencies struggle to cut costs for prescription drugs, their main tool has been the use of drug formularies--a state-approved list of thousands of drugs that the agencies will reimburse for the treatment of Medicaid patients. Now, states are trying to use a second method that could reap big savings: requiring drug companies to bid to be included in the formulary. However, both cost-containment strategies have attracted the pharmaceutical industry's attention--and their lobbyists.

Competitive Bidding↗

Big houses, big cars, superfleas and the costs of reproduction.

The assumption of costs of reproduction were a logical necessity for much of the early development of life history theory. An unfortunate property of 'logical necessities' is that it is easy to also assume that they must be true. What if this does not turn out to be the case? The existence and universality of costs of reproduction were initially challenged with empirical data of questionable value, but later with increasingly strong theoretical and empirical results. Here, we discuss Ken Spitze's 'superfleas', which represent what we consider to be the strongest empirical challenge to the universality of costs, then offer a possible explanation for their existence.

Journal Article↗

Does emotional intelligence meet traditional standards for an intelligence? Some new data and conclusions.

Performance-based measures of emotional intelligence (EI) are more likely than measures based on self-report to assess EI as a construct distinct from personality. A multivariate investigation was conducted with the performance-based, Multi-Factor Emotional Intelligence Scale (MEIS; J. D. Mayer, D. Caruso, & P. Salovey, 1999). Participants (N = 704) also completed the Trait Self-Description Inventory (TSDI, a measure of the Big Five personality factors; Christal, 1994; R. D. Roberts et al.), and the Armed Services Vocational Aptitude Battery (ASVAB, a measure of intelligence). Results were equivocal. Although the MEIS showed convergent validity (correlating moderately with the ASVAB) and divergent validity (correlating minimally with the TSDI), different scoring protocols (i.e., expert and consensus) yielded contradictory findings. Analyses of factor structure and subscale reliability identified further measurement problems. Overall, it is questionable whether the MEIS operationalizes EI as a reliable and valid construct.

Adolescent↗

[Physicians employed by municipalities--how big are the differences?].

The present paper discusses the geographical distribution of physicians employed in municipal primary care. In Norway, primary medical services are the responsibility of the local public authority (the municipality) and are financed primarily by the general taxation. The allocation of physicians is analysed using a municipal demand model. The model is a synthesis of consumers' demand and allocation of municipal funds. Analyses were performed on a panel data set of all Norwegian municipalities covering the period 1986-92. The results are encouraging, since they indicate that a decentralised system of primary medical services does seem to be fairly effective in securing the municipal population equity of access to the services. In particular, the municipalities seem to respond well to the health care needs of their population. Distribution of physicians depends to only a very small extent on the wealth of the municipality.

Community Health Services↗

Cosmic microwave background snapshots: pre-WMAP and post-WMAP.

We highlight the remarkable evolution in the cosmic microwave background (CMB) power spectrum C(l) as a function of multipole l over the past few years, and in the cosmological parameters for minimal inflation models derived from it: from anisotropy results before 2000; in 2000 and 2001 from Boomerang, Maxima and the Degree Angular Scale Interferometer (DASI), extending l to approximately 1000; and in 2002 from the Cosmic Background Imager (CBI), Very Small Array (VSA), ARCHEOPS and Arcminute Cosmology Bolometer Array Receiver (ACBAR), extending l to approximately 3000, with more from Boomerang and DASI as well. Pre-WMAP (pre-Wilkinson Microwave Anisotropy Probe) optimal band powers are in good agreement with each other and with the exquisite one-year WMAP results, unveiled in February 2003, which now dominate the l less, similar 600 bands. These CMB experiments significantly increased the case for accelerated expansion in the early Universe (the inflationary paradigm) and at the current epoch (dark energy dominance) when they were combined with "prior" probabilities on the parameters. The minimal inflation parameter set, [omega(b), omega(cdm), Omega(tot), Omega(Lambda), n(s), tau(C), sigma(8)], is applied in the same way to the evolving data. C(l) database and Monte Carlo Markov Chain (MCMC) methods are shown to give similar values, which are highly stable over time and for different prior choices, with the increasing precision best characterized by decreasing errors on uncorrelated "parameter eigenmodes". Priors applied range from weak ones to stronger constraints from the expansion rate (HST-h), from cosmic acceleration from supernovae (SN1) and from galaxy clustering, gravitational lensing and local cluster abundance (LSS). After marginalizing over the other cosmic and experimental variables for the weak + LSS prior, the pre-WMAP data of January 2003 compared with the post-WMAP data of March 2003 give Omega(tot) = 1.03(-0.04)(+0.05) compared with 1.02(-0.03)(+0.04), consistent with (non-Baroque) inflation theory. Adding the flat Omega(tot) = 1 prior, we find a nearly scale-invariant spectrum, n(s) = 0.95(-0.04)(+0.07) compared with 0.97(-0.02)(+0.02). The evidence for a logarithmic variation of the spectral tilt is less than or approximately 2sigma. The densities are for: baryons, omega(b) identical with Omega(b)h(2) = 0.0217(-0.002)(+0.002) (compared with 0.0228(-0.001)(+0.001)), near the Big Bang nucleosynthesis (BBN) estimate of 0.0214 +/- 0.002; CDM, omega(cdm) = Omega(cdm)h(2) = 0.126(-0.012)(+0.012) (compared with 0.121(-0.010)(+0.010)); the substantial dark (unclustered) energy, Omega(Lambda) approximately 0.66(-0.09)(+0.07) (compared with 0.70(-0.05)(+0.05)). The dark energy pressure-to-density ratio w(Q) is not well constrained by our weak + LSS prior, but adding SN1 gives w(Q) less than or approximately -0.7 for January 2003 and March 2003, consistent with the w(Q) = -1 cosmological constant case. We find sigma(8) = 0.89(-0.07)(+0.06) (compared with 0.86(-0.04)(+0.04)), implying a sizable Sunyaev-Zel'dovich (SZ) effect from clusters and groups; the high-l power found in the January 2003 data suggest sigma(8) approximately 0.94(-0.16)(+0.08) is needed to be SZ-compatible.

Journal Article↗

Distribution and molecular form of immunoreactive big endothelin-1 in porcine tissue.

In the present study a specific and sensitive radioimmunoassay for big endothelin-1 was developed. Half maximal inhibition of binding of radioiodinated big endothelin-1 was observed at 58 pg/tube and big endothelin-1 was detectable as low as 2 pg/tube. With this assay, the regional distribution of big endothelin-1 was determined in porcine tissue and compared to the distribution of an immunoreactive endothelin. Considerable amount of immunoreactive big endothelin-1 was observed in the aortic intima (0.84 +/- 0.094 pg/mg wet tissue; mean +/- S.D.) and the lung (0.47 +/- 0.055), but there was a low concentration in other tissue including the kidney inner medulla, which has been shown to be abundant in immunoreactive endothelin. Furthermore the molecular form of immunoreactive big endothelin-1 in aortic intima was found to be big ET-1[1-39], but the molecular form of major immunoreactive big endothelin-1 in the lung is big endothelin-1[22-39] with big endothelin-1[1-39] being minor.

Amino Acid Sequence↗