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Psychosocial correlates of dietary intake: advancing dietary intervention.

Psychosocial variables that predict dietary behavior become important targets for change in nutrition education programs. Psychosocial variables in models with higher predictability provide more effective levers to promote healthy dietary change. A review of the literature on models with psychosocial variables predicting dietary fat and fruit and vegetable consumption revealed generally low predictiveness, R2 < 0.3 (where R2 is the squared multiple correlation of the statistical model). No single theory provided models that regularly out-predicted others. When models predicted narrower categories of behavior (e.g. milk or salad consumption), predictiveness tended to be higher. Substantial problems were revealed in the psychometrics of both the independent and dependent variables. Little theory-based research has been conducted with adolescents, and the few studies done with children had low predictiveness. In order to increase the predictiveness of models, future research should combine variables from several theories, attend to the psychometrics of all variables, and incorporate variables that moderate the relationship of psychosocial to dietary behavior (e.g. genetics of taste, stage in the life course). Refinements on current research would include longitudinal designs and use of non-self-report methods of dietary behavior to supplement the self-report methods. Improved understanding of dietary behavior should lead to more effective dietary behavior change interventions.

Adolescent↗

Spontaneous long-term changes of corneal power and astigmatism after suture removal after penetrating keratoplasty using a regression model.

PURPOSE: To assess the diagnosis-based spontaneous long-term changes in corneal power and refraction with a regression model in the all-sutures-out time period following non-mechanical penetrating keratoplasty (PK). DESIGN: Retrospective non-randomized clinical trial. METHODS: setting: Clinical practice. study population: 147 eyes [47 Fuchs dystrophy (FD); 100 keratoconus (KC)] were studied after suture removal in this retrospective longitudinal study. main outcome measures: Zeiss keratometry [equivalent power (KEQ) and astigmatism (KAST)], corneal topography analysis [equivalent power (TEQ) and astigmatism (TAST)], and subjective refractometry [spherical equivalent (SEQ) and refractive cylinder (RAST)] were assessed in at least three up to 16 ophthalmologic examinations in the all-sutures-out time period. observation procedure: The time course of each target variable was analyzed in a longitudinal manner (time interval > or = 12 months) separately for each patient with a linear regression model. RESULTS: Post-keratoplasty follow-up ranged from 31 months to 10.3 years. In the linear regression model, the annual change in FD/KC showed an increase/a decrease in KEQ (0.29 +/- 0.50/-0.63 +/- 0.46 diopters, P = .02) and an increase/a decrease in TEQ (0.37 +/- 0.54/-0.69 +/- 0.49 diopters, P = .04) corresponding to a decrease/an increase in SEQ (-0.31 +/- 0.47/0.63 +/- 0.43 diopters, P = .02). KAST/TAST/RAST showed a minimal annual decrease (-0.06 +/- 0.41/-0.05 +/- 0.45/-0.06 +/- 0.41 diopters) in FD but an increase in KC (0.46 +/- 0.41/0.51 +/- 0.43/0.46 +/- 0.38 diopters) (P = .05/0.06/0.12). CONCLUSIONS: In the follow-up after post-keratoplasty suture removal, patients with FD/KC tend to develop a spontaneous myopic shift (steepening of the cornea)/hyperopic shift (flattening of the cornea). In contrast with those with FD, patients with KC should be counseled on the fact that astigmatism may increase again over time after suture removal.

Astigmatism↗

Mouse inbred strain differences in ethanol drinking to intoxication.

Recently, we described a simple procedure, Drinking in the Dark (DID), in which C57BL/6J mice self-administer ethanol to a blood ethanol concentration (BEC) above 1 mg/ml. The test consists of replacing the water with 20% ethanol in the home cage for 4 h early during the dark phase of the light/dark cycle. Three experiments were conducted to explore this high ethanol drinking model further. In experiment 1, a microanalysis of C57BL/6J behavior showed that the pattern of ethanol drinking was different from routine water intake. In experiment 2, drinking impaired performance of C57BL/6J on the accelerating rotarod and balance beam. In experiment 3, 12 inbred strains were screened to estimate genetic influences on DID and correlations with other traits. Large, reliable differences in intake and BEC were detected among the strains, with C57BL/6J showing the highest values. Strain means were positively correlated with intake and BEC in the standard (24 h) and a limited (4 h) two-bottle ethanol vs. water test, but BECs reached higher levels for DID. Strain mean correlations with other traits in the Mouse Phenome Project database supported previously reported genetic relationships of high ethanol drinking with low chronic ethanol withdrawal severity and low ethanol-conditioned taste aversion. We extend these findings by showing that the correlation estimates remain relatively unchanged even after correcting for phylogenetic relatedness among the strains, thus relaxing the assumption that the strain means are statistically independent. We discuss applications of the model for finding genes that predispose pharmacologically significant drinking in mice.

Alcohol Drinking↗

Contextual effects in difference judgments.

Manipulating stimulus spacing, stimulus frequency, or stimulus range usually affects intensity judgments. In six experiments, I investigated the locus of analogues of these contextual effects in a "difference" estimation task. When all stimuli elicited the same taste quality, stimulus distribution affected the scale values only when water was included in the stimulus set (Experiments 1-3). When the subjective ranges of two taste qualities were manipulated, different scale values were obtained for the separate qualities in the two conditions (Experiment 4). Manipulation of the expected response distribution did not affect the scale values or the responses (Experiments 5-6). It is concluded that shifts in stimulus distributions or stimulus ranges result in shifts in subjective scale values. The contextual effects can be interpreted as relative shifts of a number of gustatory continua, with water lying on a separate continuum. Proposed is a model for context-dependent judgments, consisting of four stages: stimulus classification, stimulus placement, continuum placement, and continuum projection.

Adult↗

Sucrose octaacetate tasting in a heterogeneous population of CFW mice.

Three experiments investigated the genetic underpinnings of the sucrose octaacetate (SOA) avoidance-indifference dimorphism that exists among outbred CFW mice. In the first experiment, results from 687 subjects across three generations of segregation were consistent with predictions from a single-autosomal, two-allele model, with dominance for the avoidance (Taster) phenotype. In the second experiment, heterogeneous CFW Tasters and Nontasters were mated with SWR/J (Taster) and C57BL/6J (Nontaster) inbred mice. The SWR and CFW mice are both derived from Swiss mice, and the results were consistent with the possibility that the Taster animals share an allele which is identical by descent. The second and third experiments also investigated sensitivity to SOA across an extended range of concentrations. Nontaster CFWs avoided SOA at the near-saturation 10(-3) M concentration but did not avoid any weaker concentrations. Taster CFWs avoided all concentrations down to approximately 10(-6) M SOA.

Alleles↗

Human physiology in space.

The universality of gravity (1 g) in our daily lives makes it difficult to appreciate its importance in morphology and physiology. Bone and muscle support systems were created, cellular pumps developed, neurons organised and receptors and transducers of gravitational force to biologically relevant signals evolved under 1g gravity. Spaceflight provides the only microgravity environment where systematic experimentation can expand our basic understanding of gravitational physiology and perhaps provide new insights into normal physiology and disease processes. These include the surprising extent of our body's dependence on perceptual information, and understanding the effect and importance of forces generated within the body's weightbearing structures such as muscle and bones. Beyond this exciting prospect is the importance of this work towards opening the solar system for human exploration. Although both appear promising, we are only just beginning to taste what lies ahead.

Astronauts↗

Restrictive dieting vs. "undieting" effects on eating regulation in obese clinic attenders.

This study tested predictions from restraint theory [Herman & Polivy (1984). A boundary model for the regulation of eating. In: A. J. Stunkard, & E. Stellar (Eds.), Eating and its disorders (pp. 141-156) New York: Raven Press.] and the three-factor model of dieting [Psychol. Bull. 114 (1993) 100.] using an eating regulation paradigm. Participants were 42 obese, nonbinge eaters assigned to either a weight loss group (restrictive dieters or RDs) or a group designed to eliminate dieting ("undieters" or UDs). Participants took part in an ostensible ice cream taste test with or without a preload, both before and after the weight control intervention. At pretest, restraint theory's prediction that participants would engage in counter-regulatory eating was not supported. At posttest, after 8 weeks of the dieting interventions, RDs increased and UDs decreased their intake following a preload, a pattern most consistent with the predictions of restraint theory. This counter-regulatory trend was observed in spite of a significant decrease in RDs' Disinhibition scale scores following treatment. Implications of these findings for restraint theory, the three-factor model of dieting, and relapse in obesity treatment were discussed.

Adult↗

Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste.

The detection of sweet-tasting compounds is mediated in large part by a heterodimeric receptor comprised of T1R2+T1R3. Lactisole, a broad-acting sweet antagonist, suppresses the sweet taste of sugars, protein sweeteners, and artificial sweeteners. Lactisole's inhibitory effect is specific to humans and other primates; lactisole does not affect responses to sweet compounds in rodents. By heterologously expressing interspecies combinations of T1R2+T1R3, we have determined that the target for lactisole's action is human T1R3. From studies with mouse/human chimeras of T1R3, we determined that the molecular basis for sensitivity to lactisole depends on only a few residues within the transmembrane region of human T1R3. Alanine substitution of residues in the transmembrane region of human T1R3 revealed 4 key residues required for sensitivity to lactisole. In our model of T1R3's seven transmembrane helices, lactisole is predicted to dock to a binding pocket within the transmembrane region that includes these 4 key residues.

Alanine↗

Changes in adolescents' reasons for drinking in Switzerland and associations with alcohol use from 1994 to 2002.

PURPOSE: To determine changes in the prevalence of reasons for drinking and their associations with alcohol use among adolescents in Switzerland from 1994 to 2002. METHOD: Based on national representative samples of 3792 alcohol-using students in 8th and 9th grades in Switzerland (mean age 15.5 years, SD .7), chi-square tests were used to determine changes in reasons for drinking over time. To determine associations between reasons and alcohol use and changes in these associations over time, multiple regression models were estimated separately for the frequency of alcohol consumption and drunkenness. RESULTS: Most adolescents reported drinking to celebrate special occasions, because they wanted to try alcohol, or because they liked the taste of alcoholic drinks. From 1994 to 2002, an increase in all reasons was observed. In multiple regressions, nearly all reasons were significantly related to alcohol consumption and drunkenness. With two exceptions, no interaction with the study year was found. Furthermore, the number of reasons cited by the adolescents surveyed is important: the more reasons they indicated, the more often they drank. The highest alcohol use in 2002 was found among adolescents who indicated all or nearly all reasons, a group that did not exist in 1994. CONCLUSIONS: There appears to be a new group of adolescents who indicated a high number of different reasons for drinking, who often consumed alcohol, and who were frequently drunk. Prevention approaches should be especially targeted at this new risk group.

Adolescent↗

Purification of an osmotin-like protein from the seeds of Benincasa hispida and cloning of the gene encoding this protein.

A pathogenesis-related (PR) protein was purified from the seeds of Benincasa hispida, which is a medicinal plant and a member of the Cucurbitaceae family. Purification was achieved by using a procedure consisting of an acid treatment step followed by two chromatography steps. The protein is a basic protein with molecular mass of approximately 28 kDa. The sequences of the N-terminal 30 amino acids and four peptides generated from protease digestion were determined. These sequences indicated that the protein is an osmotin-like protein (OLP). Osmotin and OLPs are members of the thaumatin-like, PR-5 family of the PR proteins. A genomic clone of the gene encoding the protein was isolated and sequenced. The predicted protein has a signal peptide of 18 amino acids, and the mature protein has a molecular mass of 24.8 kDa with an isoelectric point of 7.67. The protein has 17 cysteine residues, of which 16 appear in the same positions as those appear in the sweet-tasting protein thaumatin and several other thaumatin-like proteins. Southern hybridization analysis indicated that the gene encoding the protein is a single copy gene. A computer-generated, three-dimensional model of the protein is presented.

Journal Article↗

Traditional topological indices vs electronic, geometrical, and combined molecular descriptors in QSAR/QSPR research.

A comparison of the performance of molecular descriptors of different types was conducted. The study was concentrated on determining which descriptors are included in the best linear multivariate regression models constructed for modeling various physicochemical properties (melting point, boiling point, refractive index, molar volume, and density) and biological activities (anaesthetic activity, narcotic activity, sweetness intensity). A total of 84 molecular descriptors published over the past 2 decades was included in this study, plus some of their normalized and squared forms. It was shown that, for the estimation of physicochemical properties, the best small regression models with one to four parameters are mainly comprised of "classical" topological indices such as the Randić index, Wiener index, and molecular connectivity indices. For the correlation of biological activity, combinations of topological indices with geometrical descriptors produced regression models of the best quality. In-house developed software was used for generation of the molecular descriptors (the GROUND program) and for the statistical QSAR/QSPR analysis (the GROUNDSTAT program).

Anesthetics, Local↗

Molecular similarities in the ligand binding pockets of an odorant receptor and the metabotropic glutamate receptors.

The 5.24 odorant receptor is an amino acid sensing receptor that is expressed in the olfactory epithelium of fish. The 5.24 receptor is a G-protein-coupled receptor that shares amino acid sequence identity to mammalian pheromone receptors, the calcium-sensing receptor, the T1R taste receptors, and the metabotropic glutamate receptors (mGluRs). It is most potently activated by the basic amino acids L-lysine and L-arginine. In this study we generated a homology model of the ligand binding domain of the 5.24 receptor based on the crystal structure of mGluR1 and examined the proposed lysine binding pocket using site-directed mutagenesis. Mutants of truncated glycosylated versions of the receptor containing only the extracellular domain were analyzed in a radioligand binding assay, whereas the analogous full-length membrane-bound mutants were studied using a fluorescence-based functional assay. In silico analysis predicted that aspartate 388 interacts with the terminal amino group on the side chain of the docked lysine molecule. This prediction was supported by experimental observations demonstrating that mutation of this residue caused a 26-fold reduction in the affinity for L-lysine but virtually no change in the affinity for the polar amino acid L-glutamine. In addition, mutations in four highly conserved residues (threonine 175, tyrosine 223, and aspartates 195 and 309) predicted to establish interactions with the alpha amino group of the bound lysine ligand greatly reduced or eliminated binding and receptor activation. These results define the essential features of amino acid selectivity within the 5.24 receptor binding pocket and highlight an evolutionarily conserved motif required for ligand recognition in amino acid activated receptors in the G-protein-coupled receptor superfamily.

Amino Acid Substitution↗

An empirically derived population-response model of the short form of the Oral Health Impact Profile.

OBJECTIVE: The aim of this paper was to model the consequences of dental conditions from an empirical basis and to test the model's ability to predict response combinations. METHODS: The model was derived from responses to the short-form Oral Health Impact Profile (OHIP14) obtained from a UK population sample of 5281 dentate adults. This model was then used to predict OHIP14 response combinations obtained from a sample of 3973 dentate and edentulous adults in Australia. FINDINGS: The empirically derived population-response model accounted for over 98% of response combinations of Australian dentate adults. CONCLUSIONS: The empirically derived model followed a similar hierarchical pattern to the base model underlying the long-form version of the measure (thereby supporting the validity of the OHIP14 measure) and was strongly predictive of the pattern of responses obtained from Australian adults.

Adult↗

Factors influencing food choices, dietary intake, and nutrition-related attitudes among African Americans: application of a culturally sensitive model.

OBJECTIVES: The goals of the project were: (1) to explore how culture and community impact on the nutrition attitudes, food choices, and dietary intake of a selected group of African Americans in north central Florida; and (2) to identify segments of the population and community that should be targeted for education programs, desirable components of nutrition education programs, topics of interest, and health promotion channels to reach the target group. DESIGN: Six focus groups were conducted with African American males and females. The data were analyzed using the PEN-3 model, a theoretical model that centralizes culture as the primary reason for health behavior and the primary consideration for health promotion and diseases prevention programs. RESULTS: There was a general perception that 'eating healthfully' meant giving up part of their cultural heritage and trying to conform to the dominant culture. Friends and relatives usually are not supportive of dietary changes. Barriers to eating a healthful diet also included no sense of urgency, the social and cultural symbolism of certain foods, the poor taste of 'healthy' foods, the expense of 'healthy' foods, and lack of information. Segments of the population that potentially could be motivated to make dietary changes included women, men with health problems, young adults, the elderly, and those diagnosed with a severe, life-threatening disease. CONCLUSION: The findings suggest that the PEN-3 model is an appropriate framework for assessing how community and culture impact dietary habits of African Americans. African Americans will need information on basic nutrition topics such as serving sizes and reading food labels. The findings also suggest that programs and materials should be specifically developed for churches, neighborhood grocery stores, and local restaurants.

Adult↗