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Somatosensory evoked potential activity: a measure of interhemispheric transfer in schizophrenia?

Jones and Miller (1981) reported that schizophrenics could be distinguished from healthy controls by latency differences in their ipsilateral and contralateral somatosensory evoked potentials (SEPs), considered a measure of corpus callosum conduction time. Subsequent replication studies failed to follow the method of the original study. The present study represents a precise replication. An additional control for contamination of SEPs by an auditory stimulus generated by the vibrotactile stimulus was used. Ipsilateral SEP activity was identified but poor definition of a positive peak in the range of interest prevented measurement of peak latency. Evidence was advanced to suggest that such ipsilateral potentials result from activity at the vertex. It was concluded that this method is inappropriate for the investigation of interhemispheric transmission.

Adult↗

Individual differences in auditory electric responses: comparisons of between-subject and within-subject variability. II. Amplitude of brainstem Vertex-positive peaks.

Recently, we (Lauter & Loomis, 1986) reported variability measures of the latency of five vertex-positive auditory brainstem response (ABR) peaks collected under a repeated-measures experimental design. Seven subjects were tested, each on eight separate sessions, for brainstem auditory evoked response to monaural right, monaural left, and binaural stimulus presentation. This paper presents variability measures for amplitudes of the same series of responses. Three types of variability measurement were made: 1) amplitude of each peak of the response to monaural right, monaural left, and binaural stimulation; 2) amplitude difference for each peak comparing binaural with right, and binaural with left; and 3) amplitude difference comparing binaural with the sum of the amplitudes of the two monaural responses. As in the previous report, between-subject variability and within-subject variability were expressed using a ratio of mean divided by standard deviation (this is the reciprocal of Pearson's Coefficient of Variation, and will here be referred to as the Coefficient of Stability, or Cs). For all amplitude comparisons, Cs profiles indicate that: 1) within-subject stability (i.e., consistency) is significantly greater than between-subject stability, 2) both within- and between-subject stability measures are sensitive to both peak and ear of presentation, and 3) stability profiles for individual subjects show individual differences and similarities, and are replicable over time. The variability measure also provides evidence of an ear asymmetry at peak III which has been noted in other ABR studies.

Brain Stem↗

Effects of corn processing and supplemental hay on rumen environment and lactation performance of dairy cows grazing grass-legume pasture.

The effect of corn processing (9 kg of dry matter/d of ground dry shelled or 9 kg of dry matter/d of steam rolled) and supplemental hay (0 or 3.2 kg of dry matter/d of alfalfa hay) on milk yield and composition, rumen environment, and starch utilization by lactating cows grazing grass-legume pasture was studied. Twelve rumen cannulated, multiparous Holstein cows in early lactation (95 d in milk), were assigned to a 4 x 4 Latin square design replicated three times. Treatments were ground shelled corn-based concentrate, ground shelled corn-based concentrate plus alfalfa hay, steam-rolled, corn-based concentrate, or steam-rolled, corn-based concentrate plus alfalfa hay. Supplements were fed in equal proportions twice daily. Cows fed steam-rolled corn tended to have higher percentage of milk protein and lower milk urea nitrogen concentrations than cows fed shelled corn. Milk yield was not affected by corn processing or hay supplementation. Intake of pasture forage but not total dry matter intake was reduced by hay supplementation. Starch plus free glucose digestibility in the total tract was not affected by grain processing; however, starch plus free glucose digestibility tended to increase with hay supplementation. Supplemental hay increased starch plus free glucose digestibility through changes in rumen digestion kinetics. Hay supplementation reduced the liquid rate of passage, and tended to reduce particulate turnover. Rumen degradability of pasture forage organic matter tended to be higher for cows fed supplemental hay. Supplemental hay in these diets had a greater impact on starch utilization than corn processing.

Animal Feed↗

Effects of increasing levels of grain supplementation on rumen environment and lactation performance of dairy cows grazing grass-legume pasture.

The impact of supplemental energy on nutrient utilization, fiber digestion, rumen fermentation, and lactation performance was evaluated in dairy cows grazing pastures composed of brome, orchardgrass, red clover, and alfalfa. Three amounts [0, 5, and 10 kg dry matter (DM)/d] of ground dry shelled corn-based concentrate were supplemented to nine rumen cannulated Holstein cows in a 3 x 3 Latin square replicated three times. Cows were on average 84+/-13 d in milk and producing 41.6+/-5.9 kg of milk/d at the beginning of the study. An increase in amounts of concentrate in the diets was associated with an increase in milk production, solids-corrected milk, and concentrations of milk protein and SNF. Milk fat percentage and milk urea nitrogen concentration decreased linearly with supplementation. Milk production and protein percentage were 21.8, 26.8, and 30.4 kg/d, and 2.85, 2.95, and 3.05% for the increasing levels of concentrate, respectively. Intake and digestibility of DM and organic matter (OM) increased as grain supplementation increased. Ruminal pH and total volatile fatty acid concentration (VFA) were not affected by supplementation or the amount of concentrate. Ruminal ammonia concentration was reduced by supplementation, presumably due to a decrease in N intake and greater use of ammonia-N for rumen microbial protein synthesis. Rumen fermentation varied throughout the day, with lower mean pH and higher VFA concentrations at night. Supplementation increased total OM intake, decreased forage OM intake, and increased the proportion of OM that was digested in the intestines. Total DM intake by grazing dairy cows can be increased using ground dry shelled corn-based concentrate without causing negative effects on forage digestion.

Ammonia↗

Effect of milk preacidification on low fat mozzarella cheese: II. Chemical and functional properties during storage.

The effect of milk preacidification on cheese manufacturing, chemical properties, and functional properties of low fat Mozzarella cheese was determined. Four vats of cheese were made in 1 d using no preacidification (control), preacidification to pH 6.0 and pH 5.8 with acetic acid, and preacidification to pH 5.8 with citric acid. This process was replicated four times. Modifications in the typical Mozzarella manufacturing procedures were necessary to accommodate milk preacidification. The chemical composition of the cheeses was similar among the treatments, except the calcium content and calcium as a percentage of protein were lower in the preacidified treatments. During refrigerated storage, the chemical and functional properties of low fat Mozzarella were affected the most by milk preacidification to pH 5.8 with citric acid. The amount of expressible serum, unmelted cheese whiteness, initial unmelted hardness, and initial apparent viscosity were lower with preacidification. The reduction in initial unmelted cheese hardness and initial apparent viscosity in the pH 5.8 citric treatments represents an improvement in the quality of low fat Mozzarella cheese that allows the cheese to have better pizza bake characteristics with shorter time of refrigerated storage.

Acetic Acid↗

Effects of corn particle size and source on performance of lactating cows fed direct-cut grass-legume forage.

We conducted two experiments to evaluate the effects of corn supplementation, source of corn, and corn particle size on performance and nutrient utilization of lactating dairy cows. In experiment 1, treatments were 1) direct-cut grass-legume forage without supplement, 2) direct-cut forage plus 10 kg DM of ground dry shelled corn-based concentrate, and 3) direct-cut forage plus 10 kg DM of coarsely ground high moisture ear corn-based concentrate. In experiment 2, treatments were 1) direct-cut grass-legume forage plus 10 kg DM of ground dry shelled corn-based concentrate, 2) direct-cut forage plus 10 kg DM of coarsely ground high moisture ear corn-based concentrate, and 3) direct-cut forage plus 10 kg of DM finely ground high moisture ear corn-based concentrate. Both experiments were designed as 3 x 3 Latin squares replicated three times. In experiment 1, yields of milk and milk protein increased with concentrate supplementation, but were not affected by source of corn. Solids-corrected milk yield tended to increase with grain supplementation. Dry matter intake increased with concentrate supplementation, but was not affected by source of corn or corn particle size. Corn supplements decreased ruminal pH and acetate to propionate ratio and increased ruminal propionate concentration. Grain supplements reduced ruminal ammonia concentration, increased concentration of urine allantoin, and increased the urinary allantoin to creatinine ratio. In the second study, fine grinding of high moisture corn reduced fecal starch plus free glucose levels and tended to increase its apparent digestibility. In both experiments, starch plus free glucose intake was higher on the diets with dry corn, but its utilization was not affected by source of corn.

Animal Feed↗

Influence of dietary nonfiber carbohydrate concentration and supplementation of sucrose on lactation performance of cows fed fescue silage.

There is interest in knowing if the source of nonfibrous carbohydrates (NFC) influences milk production and composition. Our objective was to determine the effects of source (starch or sugar) and level of NFC in the diet on these parameters. A 4 x 4 Latin square replicated five times using early-lactation (56 +/- 9 DIM) Holstein cows was used; cows were offered one of two levels of NFC and either no added sucrose or sucrose substituting for 10% of the corn. Diets were balanced to meet National Research Council requirements for total protein, energy, and minerals. Tall fescue silage was included at one of two levels (0.95 or 1.25% of BW as forage NDF), resulting in diets with 40 and 30% NFC. The remaining ingredients consisted of high-moisture corn, soybean meal, SoyPlus, minerals, and vitamins. Megalac (0.45 kg) was used in the low NFC diets. High NFC diets were lower (P < 0.01) in neutral detergent fiber (NDF; 31.5%) and crude protein (CP; 19.6%) than the low NFC diet (35.8% NDF and 21.0% CP). Sucrose containing diets were somewhat lower (P < 0.01) in NDF (33.1%) than the no sucrose added diets (34.3%), but diets did not differ in CP%. Cows offered the high NFC level produced more milk (39.6 kg/d; P < 0.05) than those offered the low level (38.3 kg/d), primarily due to higher dry matter intake (P < 0.05). Cows consuming the high NFC diet also had lower (P < 0.05) milk fat (3.25%) and milk urea nitrogen (MUN; 13.7 mg/dl), and higher (P < 0.05) milk protein (2.58%) and milk lactose (4.81%) concentrations than cows offered the low NFC level (3.46% milk fat, 17.5 mg/dl MUN, 2.51% milk protein, and 4.74% milk lactose). Fat yield was higher (P < 0.05) for cows fed low NFC diets than cows fed high NFC diets, whereas protein and fat yield were lower (P < 0.05) for cows fed low NFC diets than those fed high NFC diets. The NFC source did not influence dry matter intake or milk production or milk component yield (P > 0.05). Milk lactose (4.79%) and MUN (16.0 mg/dl) concentrations were higher (P < 0.05) for cows offered sucrose as a portion of the NFC compared with those not offered sucrose (4.76% milk lactose and 15.2 mg/dl MUN). Results suggest that cows fed sucrose may utilize diet nitrogen less efficiently than those not fed sucrose, when sucrose is replacing a portion of the high-moisture corn in the diet.

Animals↗

Quantitative trait loci mapping for dairy cattle production traits using a maximum likelihood method.

A maximum likelihood method was developed for QTL mapping in half-sib designs and compared to the regression method in analyses of both field and simulated data. The field data consisted of milk production evaluations of 433 progeny tested sons of 6 sires and 64 microsatellite markers distributed over 12 chromosomes. Based on permutation tests, 5 significant QTL were detected in the field data by the regression method compared with 10 by the maximum likelihood method (P < 0.05). In field data analysis, the maximum likelihood method detected more significant QTL and had a smaller residual variance than the regression method. The simulation included 9 scenarios differing in number of families, family size, QTL variance, and marker density, each replicated 100 times. The simulation results suggested that, as for the regression method, the precision of estimating QTL from the maximum likelihood method improves with increasing number of sons per sire, increasing the ratio of QTL to phenotypic variance, and decreasing marker interval. The maximum likelihood method had a smaller dispersion of estimated QTL positions than the regression method in 6 of 9 scenarios simulated. Overall, the maximum likelihood method shows potential advantage in QTL detection over the regression method, especially in the situations with less favorable conditions for QTL detection.

Algorithms↗

Effect of feeding space on the inter-cow distance, aggression, and feeding behavior of free-stall housed lactating dairy cows.

The objectives of this study were to determine whether doubling the amount of feeding space from 0.5 to 1.0 m per cow leads to increased spacing between cows at the feeder, fewer aggressive social interactions among cows, and ultimately increased feeding activity. Twenty-four lactating Holstein cows were provided with 0.5 and 1.0 m of feeding space per cow in a 2 x 2 crossover design replicated over time. Time-lapse video was used to quantify the inter-cow distance and incidence of aggressive displacements at the feed alley. An electronic feed alley monitoring system was used to monitor the feeding behavior of the cows. When animals had access to 1.0 m per cow, there was at least 60% more space between animals and 57% fewer aggressive interactions while feeding than with access to 0.5 m of feeding space. These changes in spacing and aggressive behavior in turn allowed cows to increase feeding activity throughout the day, especially during the 90 min after providing fresh feed (an increase of 24%). This increase in feeding activity was particularly evident for subordinate cows. These results indicate that increasing space allowance at the feeder increases feeding activity and reduces competition among lactating dairy cows.

Aggression↗

Impact of milk preacidification with CO2 on cheddar cheese composition and yield.

Preacidification of milk for cheese making may have a beneficial impact on increasing proteolysis during cheese aging. Unlike other acids, CO(2) can easily be removed from whey. The objectives of this work were to determine the effect of milk preacidification on Cheddar cheese composition, the recovery of individual milk components, and yield. Carbon dioxide was injected inline after the cooling section of the pasteurizer. Cheeses with and without added CO(2) were made simultaneously from the same batch of milk. This procedure was replicated 3 times. Carbon dioxide in the cheese milk was about 1600 ppm, which resulted in a milk pH of about 5.9 at 31 degrees C. The starter culture and coagulant addition rates were the same for both the CO(2) treatment and the control. The whey pH at draining of the CO(2) treatment was lower than the control. Total make time was shorter for the CO(2) treatment compared with the control. Cheese manufactured from milk acidified with CO(2) retained less of the total calcium and fat than the control cheese. The higher fat loss was primarily in the whey at draining. Preacidification with CO(2) did not alter the crude protein recovery in the cheese. The CO(2) treatment resulted in a higher added salt recovery in the cheese and produced a cheese that contained too much salt. Considering the higher added salt retention, the salt application rate could be lowered to achieve a typical cheese salt content. Cheese yield efficiency of the CO(2) treated milk was 4.4% lower than the control due to fat loss. Future work will focus on modifying the make procedure to achieve a normal fat loss into the whey when CO(2) is added to milk.

Animals↗

Impact of milk preacidification with CO2 on the aging and proteolysis of cheddar cheese.

To determine the influence of milk preacidification with CO(2) on Cheddar cheese aging and proteolysis, cheese was manufactured from milk with and without added CO(2). The experiment was replicated 3 times. Carbon dioxide (approximately 1600 ppm) was added to the cold milk, resulting in a milk pH of 5.9 at 31 degrees C in the cheese vat. The starter and coagulant usage rates were equal for the control and CO(2) treatment cheeses. The calcium content of the CO(2) treatment cheese was lower, but no difference in moisture content was detected. The higher CO(2) content of the treatment cheeses (337 vs. 124 ppm) was maintained throughout 6 mo of aging. In spite of having almost one and a half times the salt-in-moisture, proteolysis as measured by pH 4.6 and 12% trichloroacetic acid soluble nitrogen expressed as percentages of total nitrogen, was higher in the CO(2) treatment cheeses throughout aging. The ratio of alpha(s)-casein (CN) to para-kappa-CN decreased faster in the CO(2) treatment cheeses than in the control cheeses, especially before refrigerated storage. No difference was detected in the ratio of beta-CN to para-kappa-CN between the control and CO(2) treatment cheeses. Intact alpha(s)- and beta-CN were found in the expressible serum (ES) from the CO(2) treatment cheese as well as alpha(s1)-I-CN, but they were not detected in the ES from the control cheese. No CN was detected in the ES from the curd before the salting of either the control or CO(2) treatment cheese. Higher proteolysis in the cheese made from milk preacidified with CO(2) may have been due to increased substrate availability in the water phase or increased chymosin activity or retention in the cheese.

Animals↗

Heat- and lignosulfonate-treated canola meal as a source of ruminal undegradable protein for lactating dairy cows.

This experiment used 18 lactating Holstein cows in a 3 x 3 Latin square replicated 6 times to determine the effectiveness of processing with moist heat or moist heat combined with lignosulfonate (LSO3) for increasing the ruminal undegradable fraction of canola meal for use as a protein supplement for lactating dairy cows. Diets were formulated to be isonitrogenous and contained one of 3 forms of canola meal; untreated canola meal (UCM), heat-treated canola meal (HTCM) or heat-and LSO3-treated canola meal (LSO3CM). Total collection of urine and feces was taken from each cow during the last 5 d of each 42-d experimental period. Milk production was greater for cows fed the LSO3CM diet (36.6 kg/d) than for cows fed the UCM diet (34.8 kg/d) but did not differ from cows fed the HTCM diet (35.3 kg/d). Digestibility of crude protein was lower for cows supplemented with LSO3CM and they had reduced concentrations of ruminal ammonia N, blood urea N, and milk urea N compared with cows supplemented with UCM or HTCM. Dry matter intake and apparent digestibilities of neutral and acid detergent fiber were increased in cows fed the LSO3CM diet. Urinary N excretion (as % of N intake) was reduced in cows fed the LSO3CM diet. These results indicate that moist heat combined with LSO3 treatment of canola meal was effective in increasing the proportion of crude protein digested in the lower digestive tract of lactating cows and was therefore used more effectively as a source of protein than UCM or HTCM.

Ammonia↗

Yield and aging of Cheddar cheeses manufactured from milks with different milk serum protein contents.

Whey proteins in general and specifically beta-lactoglobulin, alpha-lactalbumin, and immunoglobulins have been thought to decrease proteolysis in cheeses manufactured from concentrated retentates from ultrafiltration. The proteins found in whey are called whey proteins and are called milk serum proteins (SP) when they are in milk. The experiment included 3 treatments; low milk SP (0.18%), control (0.52%), and high milk SP (0.63%), and was replicated 3 times. The standardized milk for cheese making of the low milk SP treatment contained more casein as a percentage of true protein and more calcium as a percentage of crude protein, whereas the nonprotein nitrogen and total calcium content was not different from the control and high SP treatments. The nonprotein nitrogen and total calcium content of the milks did not differ because of the process used to remove the milk SP from skim milk. The low milk SP milk contained less free fatty acids (FFA) than the control and high milk SP treatment; however, no differences in FFA content of the cheeses was detected. Approximately 40 to 45% of the FFA found in the milk before cheese making was lost into the whey during cheese making. Decreasing the milk SP content of milk by 65% and increasing the content by 21% did not significantly influence general Cheddar cheese composition. Higher fat recovery and cheese yield were detected in the low milk SP treatment cheeses. There was more proteolysis in the low milk SP cheese and this may be due to the lower concentration of undenatured beta-lactoglobulin, alpha-lactalbumin, and other high molecular weight SP retained in the cheeses made from milk with low milk SP content.

Animals↗

Influence of calcium and phosphorus, lactose, and salt-to-moisture ratio on Cheddar cheese quality: manufacture and composition.

Eight Cheddar cheeses with 2 levels of calcium (Ca) and phosphorus (P), residual lactose, and salt-to-moisture ratio (S/M) were manufactured. All cheeses were made using a stirred-curd procedure and were replicated 3 times. Treatments with a high level of Ca and P were produced by setting the milk and drawing the whey at a higher pH (6.6 and 6.3, respectively) compared with the treatments with a low level of Ca and P (pH of 6.2 and 5.7, respectively). The lactose content in the cheeses was varied by adding lactose (2.5% by weight of milk) to the milk for high lactose cheeses, and washing the curd for low lactose cheeses. The difference in S/M was obtained by dividing the curds into halves, weighing each half, and salting at 3.5 and 2.25% of the weight of the curd for high and low S/M, respectively. All cheeses were salted at a pH of 5.4. Modifications in cheese-making protocols produced cheeses with desired differences in Ca and P, residual lactose, and S/M. Average Ca and P in the high Ca and P cheeses was 0.68 and 0.48%, respectively, vs. 0.53 and 0.41% for the low Ca and P cheeses. Average lactose content of the high lactose treatments at d 1 was 1.48% compared with 0.30% for the low lactose treatments. The S/M for the high and low S/M cheeses was 6.68 and 4.77%, respectively. Mean moisture, fat, and protein content of the cheeses ranged from 32.07 to 37.57%, 33.32 to 35.93%, and 24.46 to 26.40%, respectively. The moisture content differed among the treatments, whereas fat and protein content on dry basis was similar.

Animals↗

Utilization of Starea, urea, or soybean meal in complete rations for lactating dairy cows.

Three complete rations containing 15.8% of the dry matter as crude protein were compared to a 14.5% crude protein basal ration (dry matter basis) through eight Holstein cows in a replicated 4 times 4 Latin square design. The high protein rations contained supplemental nitrogen totally from soybean meal, urea, or Starea, the latter two providing 16 and 22% of total ration nitrogen, respectively. Concentrations were mixed with wilted alfalfa-bromegrass silage and complete rations fed ad libitum. Data were collected during the last week of each 28 days. Ration had no effect on milk yield, composition of fat or protein, or apparent digestibility of dry matter, crude protein, or acid detergent fiber. Weight gains and dry matter intake were greatest with the soybean meal ration. Intake of the low protein ration was depressed. There were no significant differences between the high protein rations for efficiency of nitrogen utilization.

Animal Feed↗

Effects of a new multielement buffer on production, ruminal environment, and blood minerals of lactating dairy cows.

Twelve multiparous Holstein cows were used to evaluate the capacity of a multielement compound consisting mainly of northupite and sylvite to alleviate low milk fat percent. Possible mechanisms of action were assessed. Cows were arranged in a 4 X 4 Latin square design replicated three times. Basal diet was 55% concentrate:45% forage fed ad libitum. Dietary treatments were control, NaHCO3 at 1% of diet DM, and multielement buffer at 1 and 3% of diet DM. Feed intake and milk production were similar for all treatments. Milk fat percentages for the four treatments were 2.97, 3.21, 3.43, and 3.67%, respectively. A shift toward a higher molar percent of ruminal acetate and a lower molar percent of valerate appeared to coincide with changes in milk fat percentage. Milk protein percentage also was increased by supplemental multielement buffer. Ruminal fluid acidity was reduced by NaHCO3 and multielement buffer. Extent of in situ digestion of forage DM and cellulose was improved when cows consumed a buffering agent. Rate of corn silage digestion tended toward improvement. As mineral buffer was consumed, concentrations of Mg and K increased in ruminal fluid and blood. As dietary Na intake increased, ruminal and plasma Cl concentrations were depressed and plasma S and Ca were elevated.

Animals↗

Effect of high fiber energy supplements on nutrient digestibility and milk production of lactating dairy cows.

Sixteen multiparous Holstein cows past peak lactation were used in a 4 x 4 Latin square design replicated four times to determine the production response and digestibility of diets containing high fiber energy supplements. Corn gluten feed, soybean hulls, or wheat middlings were substituted for a portion of corn, soybean meal, and corn silage in the control diet to provide 22% of the total dietary DM. Intake of DM was decreased when cows consumed wheat middlings compared with control and soybean hulls. Actual milk and SCM yields and milk fat percentage were not different among treatments. Milk protein percentage was greater when cows consumed corn gluten feed compared with soybean hulls. Apparent digestibility of DM was greater when cows consumed soybean hulls compared with wheat middlings. Intake and apparent digestibility of ADF and NDF were greater when cows consumed soybean hulls. Cows consuming wheat middlings had intermediate intake and decreased apparent digestibility of NDF compared with controls. Intake and apparent digestibility of NDF were not different when cows received corn gluten feed compared with control or wheat middlings. High fiber energy supplements supported milk production equally; however, differences in DM and nutrient intake, milk composition, and nutrient apparent digestibility were significant.

Animal Feed↗

Digestion kinetics of fiber: influence of in vitro buffer pH varied within observed physiological range.

In vitro buffer pH reflective of the diurnal variation in ruminal pH was evaluated for its impact on digestion kinetics of NDF from three forage sources. Alfalfa hay, bromegrass hay, and corn silage were incubated in phosphate-bicarbonate buffer solution adjusted to pH 6.8, 6.5, 6.2, 6.0, 5.8, or 5.5 using 1 M citric acid. Ash-free NDF was measured after 0, 6, 12, 18, 24, 48, 72, and 96 h of fermentation. The experiment was replicated three times; kinetic parameters of fiber digestion were estimated by logarithmic transformation, and by linear and nonlinear regression procedures. Lag in NDF digestion increased as pH fell from 6.8 to 6.5 and again when pH decreased below 6.0. Decreasing buffer pH below 6.2 dramatically reduced NDF digestion rate for alfalfa hay and corn silage but had no significant effect on NDF digestion rate of bromegrass hay. Lag in NDF digestion increased below pH 6.0 for both alfalfa and corn silage but increased only when pH fell below 6.2 for bromegrass. Results suggest that lowered pH exerts its negative effect on NDF digestion between pH 6.2 and 5.8, as evidenced by increased lag and decreased rate, and that critical pH and specific, affected component of digestion varied among forages.

Animal Feed↗