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Axial skeleton and pituitary gland in human fetuses with spina bifida and cranial encephalocele.

The purpose of this study was to investigate the axial skeleton and the pituitary gland in fetuses with spina bifida or cranial encephalocele in order to elucidate the pathogenesis of the conditions. The findings were related to former investigations performed on normal fetuses and on fetuses with anencephaly and rachischisis. Eight human fetuses from spontaneous or therapeutic abortions, 11-28 weeks of gestational age, were investigated. Radiographs were taken of the axial skeleton and histological investigation, including immunohistochemical marking for thyroid-stimulating hormone was performed on tissue blocks of the cranial base, including the sella turcica and the pituitary gland. Radiography revealed only minor malformations in the axial skeleton and not in all cases. The types of malformations resembled those seen in anencephaly and rachischisis. Histological investigations revealed severe malformations in the sella turcica region in spina bifida and minor ones in cranial encephalocele. Pharyngeally located adenopituitary gland tissue occurred in all fetuses. Anencephaly and cranial encephalocele seemingly are conditions resulting from different expressivity of the same multifactorial process of maldevelopment involving mesoderm (skeleton), neurectoderm (spinal cord and brain), and surface ectoderm (adenopituitary gland tissue). It is suggested that the molecular biological signaling between the notochord, the scleroderm, and the surface ectoderm is disturbed in spina bifida and cranial encephalocele.

Bone and Bones↗
PubMed1989

Vasoactive intestinal peptide in the human pituitary gland and adenomas. An immunocytochemical study.

Recent evidence indicates that vasoactive intestinal peptide (VIP) may be involved in normal pituitary function. Immunocytochemistry was used to localize VIP in human biopsied pituitary adenomas and postmortem anterior pituitary glands. Paraffin sections were immunostained for VIP with the avidin-biotin-peroxidase technique. Strong VIP-like immunoreactivity (VIP-LI) was observed in 16 of 17 prolactinomas, 12 of 14 growth hormone-secreting tumors associated with acromegaly, four of 12 ACTH-secreting tumors, and 14 of 18 nonfunctioning pituitary adenomas. In most cases, VIP was colocalized with the classical pituitary hormones. Six of the 18 nonfunctioning tumors had no demonstrable hormone immunoreactivity; five of these stained strongly for VIP, whereas one was negative. Of 18 normal anterior pituitaries, 12 showed strong diffuse staining for VIP throughout the gland. One pituitary with VIP-LI came from an individual who had undergone pituitary stalk transection. Double-immunoenzyme labeling and immunoelectron microscopy demonstrated VIP-LI in many lactotrophs, scattered thyrotrophs, corticotrophs, and in an occasional gonadotroph. These results suggest the following: 1) VIP is present in more than one cell type in normal and adenomatous human pituitaries; and 2) VIP may be involved in the function and development of pituitary tumors.

Adenoma↗
PubMed1976

Seasonal variations in the histology of the pituitary gland of Cirrhinus mrigala (Ham.) an Indian freshwater major carp, in relation to gonadal activity.

1. Seasonal variations in the histology of the pituitary gland of Cirrhinus mrigala (Ham.), an Indian freshwater major carp, in relation to gonadal activity has been studied during a one year period commencing, January, 1971. 2. The proximal pars distalis undergoes a great deal of seasonal variation in size, weight and nature of pituicytes. 3. The percentage composition of acidophils and cyanophils in the proximal pars distalis show a reciprocal relationship during the year. The acidophils predominate during the restitution phase of the gonads while the cyanophils preponderate among the different cell types during the breeding season. 4. The chromophobes do not show any significant fluctuation in numerical abundance in relation to gonadal activity. 5. Cyanophils of the proximal pars distalis undergo changes with change in gonadal activity Concentration of glycoproteinaceous material is extremely low during the restitution phase but high during the maturation phase. The cyanophils also undergo hypertrophy and hyperplasia during the spawning period. The latter discharge their contents and become vacuolated during the post spawning period. 6. The number and size of the globules in the cyanophils are related to gonadal activity. 7. Acidophils present in the rostral pars distalis and pars intermedia do not appear to be related to gonadal activity in C. mrigala. 8. The average weight of the gland is directly related to the state of maturation of the gonads, an observation being recorded here for the first time.

Animals↗
PubMed1978

Differences in the release of melanocyte-stimulating hormone in vitro by rat pituitary glands collected at various times during the oestrous cycle.

The release of melanocyte-stimulating hormone (MSH) into the medium during incubation and the pituitary tissue content of MSH were measured separately using pituitary glands collected from rats at various stages of the oestrous cycle. The MSH was measured by a biological assay using a synthetic alpha-MSH as standard. The release of MSH was maximal during thepro-oestrous phase and MSH content of the pituitary gland was highest during dioestrus. The influences of the tripeptide Pro-Leu-Gly-NH2, which inhibits MSH secretion in vivo, and of progesterone on the release of MSH in vitro were studied with tissue collected at various phases of the oestrous cycle. Pro-Leu-Gly-NH2 was effective in inhibiting MSH release both at pro-oestrus and oestrus but not at dioestrus. Progesterone overcame this inhibition.

Animals↗
PubMed1988

Effects of growth hormone-releasing hormone on somatotrophs in anterior pituitary gland allografts in hypophysectomized, orchidectomized hamsters.

We investigated the influences of growth hormone-releasing hormone (GHRH) on the percentage, size, and shape of somatotrophs in ectopic anterior pituitary tissue. Entire pituitary glands removed from 7-week-old male hamsters were placed beneath the renal capsules of 12-week-old hamsters that had been hypophysectomized and castrated 3 weeks previously. Beginning 6 days after each host had received a single allograft, each was injected subcutaneously twice daily with 4 micrograms GHRH in 100 microliter of vehicle or 100 microliter of vehicle for 16 days. Six hosts in each group were killed by decapitation on day 17, 16 h after the last injection. Nine normal male hamsters were also decapitated and their pituitary glands were removed. Sections of anterior pituitary tissue were stained for GH and with hematoxylin. The percentage of anterior pituitary cells that stained for growth hormone was similar in the 3 groups. In contrast, somatotrophs in grafts had a smaller mean cross-sectional area than those observed in glands in situ. This effect was reversed by GHRH. Analysis of the shape of somatotrophs in both groups of grafts disclosed that they were less circular in cross-section than those in glands in situ. The results suggest that GHRH may not play a role in maintaining the percentage of somatotrophs among anterior pituitary cells, but that it does play a role in maintaining their size.

Animals↗
PubMed1993

Osmotic stimuli attenuate vasoactive intestinal peptide gene expression in the rat anterior pituitary gland.

Studies on vasoactive intestinal peptide (VIP) in the anterior pituitary gland have shown that it is synthesized locally, physiologically regulated, and may act as a paracrine/autocrine factor. We have now investigated the regulation of anterior pituitary VIP gene expression in rats during osmotic stimulation. Both salt-loading and dehydration resulted in a progressive and marked reduction in VIP mRNA levels as determined by Northern analysis, to 10% of control levels at 14 days of salt-loading. The 1.7 and 1.0 kb VIP RNA transcripts were equally affected. Since anterior pituitary VIP is partially localized in lactotrophs we also measured prolactin (PRL) mRNA levels. In contrast to VIP, PRL mRNA levels were increased during both osmotic paradigms, the mRNA levels being significantly raised after 5 days of salt-loading to 130% of controls. Further experiments, conducted to examine the mechanism by which VIP gene expression is down-regulated during osmotic stimulation, demonstrated that dopamine and angiotensin II do not appear to be involved. The results show dissociated regulation of VIP and PRL during osmotic stimulation and provide suggestive evidence of a role for anterior pituitary VIP in the animal's osmoregulatory responses. VIP may therefore be a paracrine factor with diverse functional roles.

Angiotensin II↗
PubMed1994

Immunoreactive substance P and neurokinin A in the hypothalamus and anterior pituitary gland of Siberian and Syrian hamsters and of rats.

In this investigation the concentrations of immunoreactive substance P and neurokinin A in the hypothalamus and anterior pituitary of the Siberian hamster were compared with those in the rat and Syrian hamster. The concentrations of immunoreactive neurokinin A in the hypothalamus of Siberian hamsters were significantly higher than those of rats and Syrian hamsters, while male Siberian hamsters had similar amounts of substance P in the hypothalamus to those of male Syrian hamsters, but had higher amounts than those in male rats. However, female Siberian hamsters and significantly higher hypothalamic concentrations of both substance P and neurokinin A than did female Syrian hamsters and rats. In the anterior pituitary glands of Siberian hamsters, concentrations of substance P and neurokinin A were markedly higher than they were in rats and even more so more in Syrian hamsters. Ovariectomy further increased tachykinin concentrations in the anterior pituitary gland of female Siberian hamsters, and this was completely prevented by oestradiol replacement. Female Siberian hamsters kept under conditions of reduced photoperiod had significantly higher tachykinin concentrations in the anterior pituitary than did animals kept under daily photoperiods of 16 h light:8 h dark. The incubation of anterior pituitaries from female Siberian hamsters with a neurokinin A receptor antagonist resulted in a partial blockade of the LH and FSH release in response to LHRH. Thus, the high concentration of tachykinins present in the anterior pituitary of the Siberian hamster may have a local role in modulating the secretion or release of gonadotrophins.

Animals↗
PubMed1976

A rapid and simple micromethod to measure picograms of norepinephrine (NE) and dopamine (DA) in small samples of brain tissue and pituitary gland.

A rapid and simple procedure to determine Dopamine (DA) and Norepinephrine (NE) in small tissue samples (mug quantities) is described. The radioenzymatic assay sensitivity, defined as twice the blank, is about 0.1 ng for NE and DA. Drugs known to modify brain catecholamine metabolism were shown to alter differentially DA and NE concentration in the medial basal hypothalamus (MBH) of male rats. Different areas of the brain and the pituitary gland, namely the parietal cortex, the pre-optic region (POA), the median eminence (ME), the medial basal hypothalamus without the median eminence and the posterior pituitary contain different concentrations of DA and NE, emphasizing the importance of regional analyses in tissue amines determination.

Animals↗
PubMed1988

Ultrastructural evidence for oxytocin in the rat anterior pituitary gland.

OT is synthetized in the hypothalamus. These neurons project to the posterior lobe of the pituitary and the external zone of the median eminence. In order to localize OT in the male rat anterior pituitary we have used immunocytochemistry on ultra-thin sections in target cell(s) obtained by cryoultramicrotomy. OT-like immunoreactivity was observed in lactotropes only. No immunoreactivity was observed in gonadotropes, somatotropes, corticotropes or thyrotropes. In lactotropes, immunoreactivity was localized at the plasma membrane level, in the cytoplasmic matrix and around the secretory granules, but not in the other organelles, and in the nucleus. No reaction was observed by using either non-immune serum or anti-OT serum incubated with OT. No modification of OT-like immunoreactivity was observed by using anti-OT serum incubated with heterologous peptides. These results 1) provide immunocytological evidence for the presence of OT in the anterior pituitary gland; 2) indicate the presence of this peptide in one particular cell type, and 3) support the hypothesis that OT could have a direct participation in the regulation of the PRL release.

Animals↗