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The significance of the introduction of Aedes albopictus into the southeastern United States with implications for the Caribbean, and perspectives of the Pan American Health Organization.

The introduction and apparent infestation of Aedes albopictus into the Gulf States of Texas and Louisiana in the United States is viewed as the most singular medical entomological happening of this decade in the Americas. The implications for the Caribbean and other countries southward are serious, as the habitat described for this Stegomyia cousin of Ae. aegypti, is amply available in the Windward and Leeward islands of the Antilles. Studies from the Pacific and regions of Southeast Asia indicate that Ae. albopictus competes for the same peridomestic niches as Ae. aegypti and it has been found to be a competent vector of several dengue serotypes. In this presentation, a call is issued to the mosquito control and abatement programs in the Gulf States to take stringent measures to eliminate this new dengue vector before it becomes thoroughly established and spreads to other states as well as into countries of the Americas and into the West Indies.

Aedes↗

[Pest control].

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Insect Control↗

Field trials of Bromophos and Schering 34615 residual sprays and of cheesecloth impregnated with Bayer 39007 for control of Anopheles gambiae and A. funestus in Nigeria.

In a continuation of the World Health Organization's collaborative scheme for testing new insecticides against malaria vectors, field trials were carried out during 1965 in the forest belt of Western Nigeria near Lagos and in the Guinea savanna belt of Northern Nigeria near Kaduna, using bromophos residual spray and cheesecloth impregnated with Bayer 39007; Schering 34615 was applied as a residual spray in Lagos. Although bromophos appeared to be effective for at least five months after spraying in the Lagos area, the desired control was obtained for only one month in the Kaduna area; these differences appear to be due to differences in environmental conditions and to the ecology of Anopheles gambiae. Schering 34615 was also effective for up to five months in Lagos and was definitely superior to bromophos. Cheesecloth impregnated with Bayer 39007 gave good control of mosquitos for two and for three months in Kaduna and Lagos, respectively, but because the walls of the treated huts were only partly covered, some mosquitos avoided contact with the cloth. It is suggested that, because of the reduced toxic hazards, cheesecloth treatments may be feasible with potentially effective insecticides that are too toxic to spray.

Anopheles↗

Chemosterilization of Culex pipiens fatigans Wiedemann by exposure of aquatic stages. I. Sterilization potential of certain aziridinyl compounds.

The sterile-male technique has been considered to be a promising tool for the control of mosquito populations. Many chemosterilants are known to sterilize insects effectively in the same way as ionizing radiations. The sterilizing activity of 8 alkylating aziridinyl compounds has been assessed on the tropical house-mosquito, Culex pipiens fatigans Wiedemann. The chemosterilants employed for larval and pupal treatments were apholate, metepa, tepa and 5 diaziridinyl alkoxyphosphine oxides. In general, metepa was least toxic to the various life stages of C. p. fatigans during the treatment. Apholate was toxic only in pupal treatments while tepa was less toxic in pupal treatments and more toxic in larval treatments. Diaziridinyl compounds were moderately toxic in both larval and pupal treatments. The tepa and apholate treatments caused certain structural and moulting abnormalities in pupae and adults and also induced abnormalities in eggs and larvae.Among the polyaziridinyl compounds, non-toxic doses of apholate could produce about 100% sterility with larval treatment. Tepa was inferior to apholate, and metepa was least effective in larval treatment. Among the diaziridinyl compounds, the methyl ester was most promising. For pupal treatment, tepa was more effective than apholate and metepa at non-toxic doses, inducing almost complete sterility. The propyl and isopropyl esters of the diaziridinyl compounds also induced very high sterility in pupal treatments.

Animals↗

Target organisms.

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California↗

Residual toxicity of deltamethrin and permethrin on various surfaces for mosquito species culex pipiens molestul Forskal.

The culicine mosquito species Culex pipiens molestus Forskal were exposed by tarsal contact to various surfaces sprayed with the photostable synthetic pyrethroid preparations deltamethrin (NRDC 161) and permethrin (NRDC 143) used in the form of emulsifiable concentrates (EC) and wettable powders (WP) at doses 0.001, 0.005, 0.02, 0.1 and 0.5 g/m2. The time to appearance of the knock down effect and the residual toxicity were tested for each preparation and type of surface studied. The onset of deltamethrin and permethrin knock down effects was found to be very rapid at higher insecticide doses. The mean KT 100 value for permethrin EC at 0.5 g/m2 and for deltamethrin EC and WP at 0.1 g/m2 measured within the first month after insecticide application were almost invariably below 60 min on all the surfaces tested. Taking the 2-month interval as a sufficiently long period of operationally effective residual activity, the following insecticide doses can be recommended as optimal for the Culex mosquito control inside the dwelling space: deltamethrin EC and WP doses of 0.001 g/m2 for plywood, 0.005 g/m2 for whitewashed surface, 0.02 g/m2 for ceramics, and 0.02 (WP) and 0.1 (EC) g/m2 for limewashed surface; permethrin EC doses of 0.1 g/m2 for all types of surfaces and permethrin WP doses of 0.005 g/m2 for plywood and 0.02 g/m2 for the remaining types of surfaces.

Animals↗

Resistance to temephos, an organophosphorous insecticide, in Culex pipiens from Tunisia, North Africa.

Resistance to temephos, an organophosphorous insecticide (OP), was found to be low (2-fold) in 2 Culex pipiens populations collected in Sayada (mid-eastern Tunisia). This resistance was synergized by an esterase inhibitor (DEF). Two sets of over-produced esterases (A2-B2 and A4-B4), known to be involved in resistance, were identified in almost 50% of the examined insects. In addition, 3% of insects had an insensitive acetylcholinesterase. After selecting larvae of one of the samples (ES) with temephos for 6 generations, a 9-fold increase in resistance was observed, and all mosquitoes were found to carry esterases A2-B2 and an insensitive acetylcholinesterase. These results must be considered in future mosquito control programs, since 2 of the identified genes can lead to high resistance to several organophosphorous insecticides.

Animals↗

Resistance in some Caribbean populations of Aedes aegypti to several insecticides.

Thirty-four strains of Aedes aegypti larvae from 17 Caribbean countries were bioassayed for sensitivity to temephos, malathion, fenitrothion, fenthion, and chlorpyrifos. There were fairly high levels of resistance in Tortola (10-12-fold resistance) and Antigua (6-9-fold resistance) strains to temephos and to fenthion (Tortola, 7-10-fold; Antigua, 6-10-fold resistance). Most other strains showed some resistance to malathion, fenitrothion, and chlorpyrifos, but only moderate levels. Adult populations of Ae. aegypti--Aruba, Jamaica, Trinidad, Puerto Rico, St. Lucia, and Antigua strains--also showed moderate resistance to malathion. Mosquito control field data supported the laboratory findings. Doubling the diagnostic dosage of temephos for larval Ae. aegypti was only partially effective against a more resistant strain, and even so, the chemical lost its limited efficacy over a short period of time. Integrated strategies for Ae. aegypti control to mitigate the negative effects of insecticide resistance in the Caribbean strains are suggested.

Aedes↗