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PubMed2003

The application of a Marine Biotic Index to different impact sources affecting soft-bottom benthic communities along European coasts.

Following the European Water Framework Directive (2000/60/EC), the authors proposed, in a previous contribution, a Marine Biotic Coefficient (BC) to establish the ecological quality of soft-bottom benthos within European estuarine and coastal environments. The present study examines the application of the BC to the Atlantic (North Sea; Bay of Biscay; South of Spain) and Mediterranean (Spain and Greece) European coasts. The investigation assesses also the usefulness of the BC, in relation to different impact sources (e.g. drilling cuts with ester-based mud, submarine outfalls, heavy metals, industrial and mining wastes, jetties and sewerage works). The results obtained are consistent with those obtained using several methods and parameters, such as richness, diversity, evenness, Abundance-Biomass comparison plots and univariate and multivariate statistical analyses. The BC values provide a simple and clearly defined way to establish the ecological quality of soft-bottom benthos, complementary to the above mentioned methods.

Animals↗
PubMed1998

Extraction of incurred sulphamethazine in swine tissue by microwave assisted extraction and quantification without clean up by high performance liquid chromatography following derivatization with dimethylaminobenzaldehyde.

A rapid and reliable method using microwave energy is described for the extraction of spiked and incurred (freeze-dried and fresh) sulphamethazine residues from swine tissues/organs (muscle, liver and kidney). Incurred tissues were obtained from an abattoir and freeze-dried pig tissue reference materials were produced as part of a reference material study for the Community Bureau of References, European Communities. The extraction was achieved by irradiating the sample in methanol for 25 s in a household microwave oven, commonly referred to as microwave-assisted extraction (MAE). The extracts were analysed without clean-up by high performance liquid chromatography (HPLC) using a C18 column and detected at 450 nm after derivatization with 4-dimethylaminobenzaldehyde (DMABA) in a heated rector at 40 degrees C. The limit of quantitation was 2.5 micrograms kg-1 of wet tissues. A comparison of MAE with an homogenization technique indicated that MAE worked extremely well for freeze-dried samples, while it showed significant variation for wet tissues. No sulphamethazine was detected in retail pork meat and liver samples when analysed by the MAE technique.

Animals↗
PubMed1987

The effect of inaccuracies in death certification and coding practices in the European Economic Community (EEC) on international cancer mortality statistics.

An investigation into the effect of national death certification and coding practices on published mortality statistics in eight EEC countries is reported. Doctors in each country were asked to complete specimen death certificates for a bank of written case histories. Certificates from each country were coded by their own offices and then by a WHO reference centre. Within and between countries, discrepancies occurred both in the doctors' diagnoses and in the codes assigned to certificates. At an international level these differences had serious implications for the comparability of mortality data for cancers of the cervix and uterus, and for mesotheliomas.

Cause of Death↗