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PubMed1992

[Informativeness of the methods of early clinical laboratory diagnosis of acute pancreatitis].

In 92 patients with acute pancreatitis, the informative value of the available methods for laboratory investigations (general blood analysis, biochemical blood analysis with measurement of the level of total protein, bilirubin, glucose, ALAT, ASAT, amylase activity, amylase activity in the urine) was analysed. The authors consider that the indices studied are rather informative for independent reliable diagnosis of acute pancreatitis and differentiation of its forms.

Acute Disease↗
PubMed1981

[Laboratory diagnosis of viral diseases of the skin (author's transl)].

In order to establish the diagnosis of viral diseases with clinical manifestations in the skin, frequently the help of a virus laboratory is needed. Not even classical childhood diseases such as measles and rubella can safely be diagnosed by the clinician. Today, in many cases diagnosis is possible within hours after the arrival of clinical specimens in the laboratory. Only in cases of enterovirus infection and tropical diseases has the virologist to resort to the classical time-consuming methods of direct (virus isolation) or indirect (serologic) diagnostic virology.

Antibodies, Viral↗
PubMed1999

Chronic fatigue syndrome and/or fibromyalgia as a variation of antiphospholipid antibody syndrome: an explanatory model and approach to laboratory diagnosis.

Chronic Fatigue and/or Fibromyalgia have long been diseases without definition. An explanatory model of coagulation activation has been demonstrated through use of the ISAC panel of five tests, including, Fibrinogen, Prothrombin Fragment 1+2, Thrombin/ AntiThrombin Complexes, Soluble Fibrin Monomer, and Platelet Activation by flow cytometry. These tests show low level coagulation activation from immunoglobulins (Igs) as demonstrated by Anti-B2GPI antibodies, which allows classification of these diseases as a type of antiphospholipid antibody syndrome. The ISAC panel allows testing for diagnosis as well as monitoring for anticoagulation protocols in these patients.

Antiphospholipid Syndrome↗
PubMed2004

Respiratory virus infections in Stockholm during seven seasons: a retrospective study of laboratory diagnosis.

A retrospective analysis of the virological findings in all respiratory samples (7303) analysed at the laboratory of Karolinska Hospital between 1993 and 2000 was performed. The findings were studied according to age and seasonal variation, and the methods were evaluated. Most samples were from children. RSV was the dominant agent, found in 34% of all samples from children 0-1 y of age. Influenza A was found in 13% of samples from the age group 2-5 y. Influenza A dominated among adults and the elderly. RSV was found only in 2% of samples from patients 81 y or older. Adenovirus was found among children and adults, but not at all among the elderly. Both antigen detection and virus isolation were performed on 79% (5776) of the samples. For diagnosis of influenza A, virus isolation was more sensitive than immunofluorescence, but for diagnosis of RSV immunofluorescence was more sensitive than virus isolation. Thus, the analysis verified that influenza A is common not only among adults and the elderly, but also among small children. RSV was an uncommon finding among the elderly. Immunofluorescence is sensitive and rapid for the diagnosis of particularly RSV among small children and influenza in all age groups.

Adolescent↗
PubMed2003

Laboratory diagnosis of amebiasis.

The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of E. histolytica, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal E. histolytica. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples.

Animals↗