Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Complement Fixation Tests”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 901 records · Page 50Linked to original sources
PubMedDate not supplied

[The rate constant K37 in complement fixation tests: kinetic studies on the influenza virus-immune complex model (author's transl)].

A kinetic study of the fixation of complement by the influenza immune complex was performed, using an automatic method originally described by Vargues for use in immunobacteriology. The autoanalyser provided curves which could be transformed into a simple equation. The experimental graph of the complement fixation reaction is superposable on a model curve obtained by exponential dilurion of complement. The methematical formula of this exponential complement dilution fits results of complement fixation by the immunecomplex. The rate constant can be estimated from the tangent at the orgin of the curve; it is a function of the nature of the immunesystem, the quantity of immune-complex and the temperature. These results show that the reaction behaves according to the equations of first order kinetics. This law is therefore the same as that described for other immunological systems, such as the seroneutralization of bacteriophage or the fixation of isoagglutinins on red cells. The kinetic study leads to the proposal of the k37 value for the titration of an anti-influenza serum. It is the rate constant of the reaction at 37 degrees C and is independant of the time and temperature of the fixation system as it is of the initial quantity of complement. Increased sensitivity of the reaction does not yield by higher values of k37 but affords greater precision in its calculation. This approach is identical to the one proposed by Adams for the titration of anti-phage sera.

Antigen-Antibody Complex↗
PubMed1986

Comparison between magnetic enzyme-linked immunosorbent assay (MELISA) and complement fixation test (CF) in the diagnosis of paracoccidioidomycosis.

MELISA and CF were compared using sera from paracoccidioidomycosis patients before treatment and patients undergoing antimycotic treatment. With MELISA it was possible to distinguish different antibody levels in both groups of patients whereas such distinction was not observed by using CF tests. MELISA is thus an advantageous alternative to CF in the diagnosis of paracoccidioidomycosis, including the possibility of testing sera with anticomplementary activity.

Complement Fixation Tests↗
PubMed1976

Aggregation and anticomplementary activity of an antigen used in the complement fixation test for Johne's disease.

Exhaustive lipid extraction of the Johne's bacilli before preparing the Maltaner-Wadsworth antigen removed both the anticomplementary activity of the preparation and its ability to fix complement. Gel filtration temporarily removed anticomplementary activity but tended to reduce the antigenic activity of the extract. Sonication significantly reduced the anticomplementary activity of the antigen without affecting its capacity to fix complement but the effects were only temporary. It is suggested that the antigen exists as micelles which gradually aggregate and re-arrange thereby exposing anticomplementary sites hitherto masked in the dispersed micelles.

Animals↗