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[Iron deficiency and anemia in female athletes--causes and risks].

Iron deficiency is probably the most common nutrient deficiency in the western world. Low levels of iron in the body are caused by several mechanisms, and become symptomatic with the onset of iron deficiency anemia. Athletes are a special group with additional reasons for iron or blood loss, such as plasma expansion, increase perspiration, 'foot strike hemolysis, and occasionally--malnutrition. Female athletes have yet another source of blood loss--menstruation. However, the most common cause for low hemoglobin levels in an athlete is dilutional pseudoanemia, which is caused by exercise-induced fluid retention. Athletes are more sensitive to the effects of anemia and iron deficiency, as exercise performance depends on maximal oxygen carrying capacity to the active muscle, and efficient oxygen utilization. Iron deficiency without anemia can also reduce athletic performance. Diagnosis is ultimately made by a blood count and red blood cell parameters, with ferritin serving as an index of body iron stores. Treatment requires iron supplements, as it is nearly impossible to refill the iron stores through diet alone.

Anemia, Iron-Deficiency↗

Guidelines for transfusion of erythrocytes to neonates and premature infants. Fetus and Newborn Committee, Canadian Paediatric Society.

The transfusion of erythrocytes to neonates and premature infants is common and should be minimized through a reduction in the number of blood samples taken for laboratory tests. The risks to patients have been minimized with current blood-banking techniques, although neonates and premature infants may require special consideration. The indications for transfusion of erythrocytes to neonates include the presence of shock, a loss of 10% or more of the blood volume within 72 hours when further blood sampling is expected, a hemoglobin level of less than 130 g/L in neonates with cardio-respiratory disease who require increased oxygen-carrying capacity, and a hemoglobin level of less than 80 to 100 g/L in neonates with tachypnea, tachycardia, recurrent apnea, poor feeding or failure to gain weight. The attending physician should consider these indications along with clinical judgement to ensure safe and effective erythrocyte transfusion.

Blood Component Transfusion↗

Proof of homologous blood transfusion through quantification of blood group antigens.

BACKGROUND AND OBJECTIVES: Athletes may illegally enhance endurance performance by transfusing homologous red blood cells (RBCs) and thereby increasing the oxygen carrying capacity of their blood. Detecting this dangerous practice is difficult by currently used methods. The aim of this work was to develop tests capable of detecting a mixed red cell population by flow cytometry, utilizing the likelihood of differences in minor blood group antigens. DESIGN AND METHODS: Twelve antisera directed against blood group antigens, derived from donor plasma, were used in conjunction with a secondary antibody directly conjugated with fluorescein to label IgG-coated RBCs. Optimal concentrations of RBCs and antibodies were determined on panel cells used in blood banking for the identification of specific antibodies. Blood samples from 25 patients purportedly transfused with 1-3 units of RBCs were screened for evidence of transfusion, and the percentages of antigen-positive and antigen-negative red cells were automatically calculated by the software installed in the flow cytometer after setting gates around these populations on histograms of fluorescence. RESULTS: Mixed RBC populations were identified in 22 of 25 patients tested. The three patients with antigenically homogeneous populations of RBCs were subsequently found not to have received their scheduled transfusions. INTERPRETATION AND CONCLUSIONS: This technique can detect small (<5%) populations of cells that are antigenically distinct from an individual's own RBCs. These results show the potential for flow cytometry to identify illicit homologous blood transfusion in athletes, and suggest the risk of false positives may be low.

Adult↗

[Is transfusion of a single dose of concentrated erythrocytes poor clinical practice?].

The aim of the study was to determine whether a single unit of red blood cell (RBC) transfusion is a true criterion of poor patient care. Medical records of 148 patients, 76 (51.4%) males, 72 (48.6%) females, mean age 66.88 +/- 14.56 years who have received a single unit RBC transfusion at the Department of Medicine in the period from 1997 to 2000 were retrospectively studied. Pretransfusion mean hemoglobin (Hb) value (Hb1) was 85.0 +/- 12.0 (mod 78) g/L, increasing to (Hb2) 96.9 +/- 11.8 (mod 90) g/L after transfusion and the last measured value (Hb3) was 98.2 +/- 13.4 (mod 102) g/L. Six patients (66.6%) of 9 with decrease in Hb3 value (group A) died, 13 patients (35.1%) of 37 with raise in Hb3 level < 5 g/L (group B) and 5-10 g/L (group C) died during the hospital stay vs. 7 patients (6.8%) of 102 with Hb3 level raise > 10 g/L (group D). The differences between the Hb2 and Hb1 values were significant considerably from the differences between the Hb3 and Hb1 values (p = 0.001), as well as the difference in the distribution of differences between Hb2 and Hb1 (p 0.028) and Hb3 and Hb1 (p = 0.001) according to the disease outcome and the difference in mortality of men and women (p = 0.021). 38.4% of the deceased patients were from the group of coronary syndrome and/or heart failure. Statistically significant were the differences between the arterial oxygen tension (PaO2) at Hb1 and Hb2 (p = 0.0001), as well as between the arterial oxygen saturation (SaO2) at the Hb1 and Hb2 values (p = 0.0001). There is no strict clinical indication for a single unit of RBC transfusion. Determinants of the need and amount of RBC transfusion except hemoglobin value include the etiology and duration of the anemia, the patient's ability to compensate for decreased oxygen carrying capacity and tissue oxygen requirements. The majority of a single unit RBC transfusion was appropriate for the clinical situation. Our data indicate that the use of smaller volumes of allogenic RBC transfusion was not a bad clinical practice.

Aged↗

Case report: exacerbation of hemolytic anemia requiring multiple incompatible RBC transfusions.

RBC transfusions in a patient with a history of autoimmune hemolytic anemia (AIHA) can represent both a laboratory and a clinical challenge. The development of high-titer low-avidity antibodies and antibodies to high-frequency antigens may further impair the ability to identify compatible donor RBCs. Not infrequently, incompatible RBCs must be used and the desire to increase oxygen carrying capacity conflicts with the desire to avoid exacerbating the autoimmune hemolytic process with RBC transfusions. A 66-year-old Caucasian female with coronary artery disease and a history of refractory AIHA had recently developed anemia and required multiple RBC transfusions. The patient had maintained adequate RBC counts with erythropoietin and prednisone therapy for the previous 16 months. With the recent worsening of her hemolytic anemia, she had developed angina that was treated with RBC transfusions in an outpatient setting. However, her angina increased as her RBC counts decreased, leading to hospital admission for further management of her hemolytic anemia and angina. She subsequently required multiple incompatible RBC transfusions despite increased prednisone therapy and did not improve until after coronary artery stent placement and high dose IVIG therapy. This case demonstrates the usefulness of early patient phenotyping in a case of accelerating hemolytic anemia to aid in donor RBC selection, the value of communicating with clinicians and the patient regarding the use of least-incompatible RBCs, and the importance of optimizing the patient's clinical condition to avoid ischemia. In addition, it demonstrates the value of repeated attempts with IVIG treatment despite previous refractoriness to this treatment.

Aged↗

[Anesthesiologists at the initial stage of postpartum hemorrhage].

Adequate management of postpartum hemorrhage (PPH) requires, after early recognition, four components undertaken simultaneously: communication, resuscitation, monitoring, investigating the cause of bleeding, arresting the bleeding. Early recognition of PPH relies on either a perceived or a quantified blood loss of 500-1000 ml and over (using a collecting bag), or the presence of clinical signs of shock (hypotension, tachycardia, tachypnea, altered mental status). Precise timing of the diagnosis of PPH must be must annotated. Once the diagnosis is established, appropriate senior professionals, including the anesthesiologist at the early stage, must be called in. The cornerstones of initial resuscitation are restoration of blood volume and oxygen carrying capacity, achieved, with adequate intravenous access, initially with cristalloids, followed by colloids, associated with oxygen by face mask. Initial resuscitation is performed and its efficiency is assessed by continuous monitoring, hemoglobin bedside tests (Hemocue), full blood count and clotting screen. Oxytocine (Syntocinon) efficacy and side-effects are evaluated by anesthesiologists. Adequate anesthetic technique must be performed to permit to the obstetricians to perform a diagnostic procedure (manual removal of placenta, uterine revision), and to most frequently obtain arrest of bleeding. Hemodynamic stability is required for loco-regional anesthesia, while on the other hand, general anesthesia with crush induction and intubation will be performed. Anesthesiologists must anticipate in case of failure of initial measures, including in alerting the blood transfusion service and calling for additional help.

Anesthesia, Obstetrical↗

Effect of preirradiation of transplantation site on growth characteristics and hypoxic fractions in human colon tumor xenografts.

The volumetric growth curves and hypoxic fractions of seven different human colon tumor lines (clone A, clone D, WiDR, SW480, SW620, DLD-2, and HCT-8) xenografted into the flank regions of either unirradiated nude mice or mice that had received 17.5 Gy of 250-kVp X-rays 1 day prior to implantation were biomathematically analyzed using the Verhulstian equation. Significant variation was found among tumors with respect to both initial growth rates (r, days-1) and theoretical final volumes (carrying capacities, K, mm3). In radiation-damaged normal tissue, tumors grew relatively well for about the first 2 wk postimplantation, attaining volumes of about 70 to 155 mm3. Then, tumor growth rates altered. This effect varied from relatively minor effects on growth rate (tumors of clones A and D) to inhibition of growth, with actual decreases in tumor volume (e.g., WiDr, SW480, SW620, HCT-8, and DLD-2). After this short-term transience in growth kinetics, neoplasms began to steadily regrow at about 3 wk postimplantation, albeit at a slower rate than that seen in controls. Tumor bed effect values were calculated using the ratio of times at which control tumors and tumors growing in the radiation-injured tissue reached a volume of 7.5% of the K values derived from the respective control growth curves. Values for clone D, clone A, and WiDR, SW480, SW620, DLD-2, and HCT-8 tumors were, respectively, 1.89, 2.41, 3.48, 3.62, 2.82, 3.66, and 3.65, indicating that tumor bed effect responses varied by almost 100%, even for cancers of the same neoplastic class. Also, the hypoxic fractions of all tumors growing in radiation-damaged sites were increased as compared with levels in controls.

Animals↗

[Blood boosting today].

The last Winter Olympics and recent cycling events have once again called attention to the use by endurance sportsmen and women of a variety of approaches designed to increase the blood hemoglobin concentration and oxygen-carrying capacity. An increased hemoglobin concentration and maximal oxygen uptake can be achieved by training at high altitude or by "living high and training low". It can also be procured by "blood boosting" with erythropoietin or darbepoietin, blood infusion, or misuse of hemoglobin-based oxygen carriers. This paper describes methods of increasing oxygen transport, with the accent on blood infusion, which is again relevant today with the development of a test able to detect recombinant human erythropoietins.

Altitude↗

[Bioinformation processing on the developing brain].

Applying a multidimensional autoregressive model to some bio-phenomena (i. e. EEG, EMG, heart rate variability and postural ways, etc.), the dynamic mono- and multivariate higher-order activities, each of which consisted of some first- and second-order component activities, can be described in frequency and time-pattern as the power spectral density or frequency responses and impulse responses. Some of these activities were manifested in the brain and cardio-respiratory systems during ontogenic development. Along with these changes of activities, there was a change in the information-carrying capacity of the brain during ontogeny. This implies that the entropy of EEG activity diminishes during maturation of the brain. Modern mathematical techniques, developed in the area of information theory, are invaluable. It seems reasonable to assume that along these lines we shall achieve further progress in research on normal and impaired brain mechanisms and behavior in the human infant and child.

Adolescent↗

[Artificial blood].

To prepare for shortage of blood components and to avoid side effects such as blood borne infectious disease, blood substitutes such as artificial red cell (artificial oxygen carrier) and artificial platelet are being developed. As for oxygen carriers, there are several candidates such as perfluorochemicals, modified hemoglobins and liposome encapsulated hemoglobins and albuimin heme. Perfluorochemicals have limited oxygen carrying capacity and oxygen inhalation is mandatory when they are used. Modified hemoglobins such as intermolecular or intramolecular cross linked hemoglobins have side effect to cause hypertension by scavenging nitro oxide (NO) which is produced by endothelial cells, because the size of these hemoglobins are small enough to go to the adjacent place near endothelial surface. Hemoglobin vesicles (HbV) in which hemoglobins are encapsulated in liposome is most possible candidate for oxygen carrier. Usefulness and safety of the HbV is evidenced by animal shock model or exchange transfusion model and they are now being prepared for clinical trials as red blood substitutes or oxygen therapeutics. Albumin heme in which recombinant human serum albumin incorporating synthetic heme is thought an ideal resuscitation fluid as this material has colloid oncotic pressure. Short time storage and viral infection are serious concern in platelet transfusion therapy for bleeding thrombocytopenic patients. Adhesion of the platelet to the collagen surface and aggregation at the bleeding sites to plug holes in blood vessels, and to facilitate the function of the remaining platelets is a starting point in developing platelet substitutes and several platelet substitutes have been proposed on this theory.

Albumins↗

Myocardial ischemia as a result of severe benzodiazepine and opioid withdrawal.

UNLABELLED: Long-term infusion of benzodiazepines and opioids is strongly associated with dependence and withdrawal syndromes. We report the first case of severe benzodiazepine and opioid withdrawal resulting in transient myocardial ischemia. CASE REPORT: A 6-month-old female born at 25 weeks gestation with severe opioid and benzodiazepine dependence resulting from multiple operative procedures and chronic ventilatory support was receiving continuous intravenous infusion of fentanyl and midazolam after trials of enteral methadone and diazepam had been unsuccessful due to gastric intolerance. On postoperative day 5 following Nissen fundoplication and gastrostomy tube placement, she acutely developed tachycardia, hypertension, agitation, loose stools, and yawning. Attempts to provide boluses of benzodiazepines and opioids revealed a very sluggish port in her subclavian central venous catheter. Prompt replacement of the catheter occurred without complication. After resuming infusions and providing additional sedatives and opioids, the loose stools, yawning, and agitation resolved. However, the tachycardia persisted. A 12-lead ECG was notable for significant ST depression in anterior leads. Laboratory studies revealed significantly elevated cardiac enzymes. The patient was transfused with packed red blood cells to optimize oxygen-carrying capacity. Echocardiography demonstrated a small region of dyskinetic apical endocardium. Cardiac enzymes normalized within 48 h. The ECG and echocardiographic findings fully resolved after approximately 70 h. DISCUSSION: We believe that the sluggish central venous catheter port limited delivery of the midazolam and fentanyl to our patient. The resultant tachycardia and hypertension limited diastolic filling of the coronary arteries, resulting in myocardial ischemia. As the withdrawal was treated, heart rate and blood pressure returned to baseline, myocardial perfusion normalized, and the ST depression and the cardiac enzyme values normalized. This report underscores the significant morbidity associated with withdrawal syndromes and the need to recognize withdrawal early and to treat it aggressively.

Adjuvants, Anesthesia↗

Effect of acute reduction in oxygen transport on parameters of aerobic function during exercise.

The binding of haemoglobin by carbon monoxide reversibly decreases the blood O2 carrying capacity, providing a useful model of impaired circulatory O2 transport. We evaluated noninvasive parameters of aerobic function during exercise to detect small changes in O2 transport, using carbon monoxide. Ten normal subjects performed both symptom-limited incremental and two levels of constant work rate on a cycle ergometer while breathing air and air with added carbon monoxide to cause carboxyhaemoglobin (COHb) to be approximately 11% (level of heavy cigarette smoker) and 20%. Maximal O2 uptake (VO2 max), the anaerobic threshold (AT) determined from the plot of CO2 output as a function VO2 (V-slope, the ratio of increase in VO2 to work rate increment (delta VO2/delta WR) and the upper slope of the V-slope analysis were measured while progressively increasing work rate. These changed in approximately the same percent as the increase in COHb. For the constant work rate tests, the time constant of VO2 and the difference in VO2 at six minutes as compared to three minutes of exercise (delta VO2 (6-3)) were significantly increased when COHb was increased. These noninvasive parameters of aerobic function, determined from the cardiopulmonary response to incremental and constant work rate exercise, particularly when used in combination, proved to be sufficiently sensitive to objectively detect small changes in O2 transport to the working muscles during exercise.

Adolescent↗

Serum amyloid A changes high density lipoprotein's cellular affinity. A clue to serum amyloid A's principal function.

The affinity of high density lipoproteins (HDL), or HDL carrying serum amyloid A (HDL/SAA), for hepatocytes or peritoneal macrophages was examined, as part of an investigation exploring the principal function of SAA and how this may be related to amyloidogenesis. The binding results in conjunction with SAA's existence primarily on HDL during inflammation, and HDL's known "reverse cholesterol transport" function suggest a clear role for SAA in the afferent arm of the reverse cholesterol transport pathway during the process of inflammation. The presence of SAA reduced HDL's affinity for normal hepatocytes by a factor of 2. In contrast, HDL/SAA had a 3- to 4-fold higher affinity for macrophages than HDL alone. Furthermore, the number of binding sites for HDL/SAA increased on macrophages during inflammation, while decreasing on hepatocytes. The net effect was a significant shift in HDL cholesterol carrying capacity towards the macrophage. Competition experiments demonstrated that HDL/SAA is only half as effective as HDL in inhibiting radiolabeled HDL binding to macrophages. This is in keeping with the reduced apolipoprotein A-1 content in HDL/SAA. Strikingly, although HDL contains twice as much apolipoprotein A-1 as HDL/SAA, it is only one-tenth as effective as HDL/SAA in inhibiting radiolabeled HDL/SAA binding to macrophages. The latter results suggest that there is a specific SAA binding site on macrophages.

Animals↗

Placental umbilical cord blood transfusion in transfusion-dependent beta thalassemic patients: a preliminary communication.

The term blood substitute is actually a misnomer because only a part of the total functions of the blood is replaced by any available so-called substitute, i.e., oxygen delivery and volume expansion only. Therefore, a more accurate term should be red cell substitute. Cord blood, because of its rich mix of fetal and adult hemoglobin, high platelet and WBC counts, and a plasma filled with cytokine and growth factors, as well as its hypoantigenic nature and altered metabolic profile, has all the potential of a real and safe alternative to adult blood during emergencies due to any etiology of blood loss and anemia. Our experience of 92 units of cord blood transfusion in patients with beta thalassemia with severe anemia (hemoglobin concentration varying from 3.5 to 5.9 g/dl with mean hemoglobin 4.6 g/dl) proved to be extremely effective in 14 patients as an emergency substitute of adult conc RBC transfusion (male: female ratio 1:1, age varying from 6 months to 38 years). In the present series, the collection of the blood varied from 57 ml-136 ml mean 84 ml +/- 7.2 ml SD, median 87 ml, mean packed cell volume 45 +/- 3.1 SD, mean hemoglobin concentration 16.4 g/dl +/- 1.6 g/dl SD. After collection the blood was immediately preserved in the refrigerator and transfused within 72 hours of collection from the consenting mother undergoing lower uterine cesarean section. We did not encounter a single case of immunological or non immunological reaction. We suggest that the medical fraternity use this precious gift of nature, which is free from infection, hypoantigenic with an altered metabolic profile, filled with growth factor and cytokine filled plasma with potential higher oxygen carrying capacity than for adult blood, as an emergency source of blood for the management of transfusion-dependent beta thalassemics.

Adolescent↗

Haematological responses to exposure to sublethal concentration of cadmium in air breathing fish, Channa punctatus (Bloch).

The effect of sublethal concentration (29 mg/l) of cadmium on haematological parameters of Channa punctatus after the exposure periods of 7, 15 and 30 days were studied. In treated groups of cadmium exposed fish have been found to significantly decreased in total erythrocyte count, haemoglobin content, packed cell volume, mean corpuscular haemoglobin concentration and oxygen carrying capacity of blood, and at the same time the white blood cell count, mean cell volume and mean corpuscular haemoglobin were found to significantly increased. Both increasing and decreasing of the above mentioned haematological characteristics of blood cells of cadmium intoxicant are mainly with time dependent effects. With regard to the pathological characteristics of blood cells of cadmium intoxicant fish shows fragility and rupture of erythrocytic membrane and leads to haemolysis, which are very severe on 30 day of exposure group.

Animals↗

[Effect of human plasma on preservation of red blood cells].

In order to study whether plasma can affect the structure and function of red blood cells during their storage period, the differences of pH value, concentration of K(+), Na(+), osmotic fragility, plasma hemoglobin, AchE, ATP, 2.3-DPG, P50 in suspended RBC, washed RBC, and RBC with various plasma volume at different storage times were compared. The results showed that plasma helped the blood to keep the RBC at high pH value, low K(+), high Na(+) and maintain RBC-ATP, oxygen carry capacity and deformability, but no effect on maintenance of osmotic fragility, and levels of plasma hemoglobin, AchE, ATP and 2.3-DPG was found in preservated blood. In conclusion, human plasma may be in favour of the preservation of red blood cells.

2,3-Diphosphoglycerate↗

[The investigation of ischemic myocardial damage in patients with carbon monoxide poisoning].

OBJECTIVE: Poisoning with carbon monoxide (CO) due to flash gas heater or stove being an important health problem in our region, causes significant pathologies in the body as well as death by decreasing oxygen-carrying capacity of blood. This study was planned to assess whether or not myocardial damage occurs in patients with CO poisoning. METHODS: Forty consecutive adult patients with CO poisoning (30 females and 10 males) were included in this study. The mean age of the patients was 28.5+/-9.9 years (range: 15-56). The demographic characteristics, vital signs, the origin of CO gas, risk factors for coronary artery disease (CAD) and smoking habit of the patients were recorded. The evaluation of Glasgow Coma Scale score, electrocardiogram (ECG), peripheral arterial blood gases, complete blood count, creatine kinase (CK), creatine kinase-myocardial band (CKMB), troponin-T measurements were performed in all cases. Additionally, myocardial perfusion SPECT was performed in three cases with carboxyhemoglobin (COHb) levels over 50%. RESULTS: Sinus tachycardia, was observed in 9 cases. Ventricular bigeminy was seen in a case with troponin-T positive test. Six of 40 cases (15%) had significantly increased CK and CK-MB levels with normal troponin-T measurements. High troponin-T levels (0.13 ng/ml) were detected only in 1 case with COHb level of 61.3%. Myocardial SPECT was performed in 3 cases with COHb levels higher than 50% and no images compatible with defects could be identified. CONCLUSION: The results suggested that significant myocardial damage and life-threatening cardiac hemodynamic changes do not develop in CO-poisoned patients with COHb level below 60 % and without any known underlying CAD. It is not necessary to routinely measure CK, CK-MB and troponin-T, and perform myocardial perfusion SPECT in acute CO poisoning cases without any ECG abnormality, ischemic cardiac symptoms or known CAD.

Adolescent↗

Methemoglobinemia: nitrate toxicity in rural America.

Nitrates are frequently found in vegetables and ground water. Nitrate levels in ground water have increased over the past two decades because of the heightened use of nitrogenous fertilizers. Following ingestion, nitrates are converted to nitrites by fecal organisms. Nitrites are absorbed and form methemoglobin, which interferes with the oxygen-carrying capacity of hemoglobin. Infants are particularly susceptible to nitrate poisoning because fetal hemoglobin is more readily oxidized to methemoglobin. In infants, the most common source of nitrate exposure is well water, which is mixed with infant formula. Affected infants may present with asymptomatic cyanosis, which can progress to dyspnea and lethargy or coma. Blood methemoglobin concentrations are elevated. Treatment consists of the administration of oxygen and intravenous and oral methylene blue.

Adult↗