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[Photosynthetic development of purple sulfur bacteria during illumination with green light].

The photosynthetic purple sulphur bacterium Chr. vinosum grows by assimilating carbon dioxide at the account of the energy of light of different spectral composition. Short wavelengths of physiological radiation, blue and green, the region of carotenoid absorption, as well as white light, are used by the bacterium for assimilation of carbon dioxide, biosynthesis of biomass, protein, and pigments. Therefore, the possibility of utilization of the energy of green light for bacterial photosynthesis was shown for the first time. Blue light is more favourable for growth of the bacterium than green light is, provided the energy (in ergs or incident quanta) is the same. An increase in the intensity both of long and short wavelength radiation activates biomass accumulation and CO2 assimilation. Photosynthetic growth of the bacterium during its illumination with wavelengths of 464, 497, and 535 nm etc. which are similar to the absorption maxima of carotenoid pigments, suggests the participation of the latter in the uptake of energy that is necessary for photosynthesis.

Bacterial Proteins↗

Cellular content of storage inclusions in purple sulfur bacteria determined by ultrathin sections.

Phototrophic sulfur bacteria accumulate storage inclusions as a mechanism to adapt to several types of environmental stress. We compare the content of elemental sulfur and poly-beta-hydroxybutyrate (PHB) found in cultures growing under laboratory conditions with the content found in microorganisms in natural environments. Since natural communities are extremely heterogeneous in composition (they do not contain only phototrophic sulfur bacteria) and physiological state, it was not possible to apply conventional chemical analyses for the determination of the contents of sulfur and PHB. The study was performed by means of transmission electron microscopy, which turned out to be an excellent tool for this purpose. The results indicate that, in natural environments, cells have an extremely high content of storage inclusions, much higher than their laboratory grown counterparts, probably as a consequence of less favorable environmental conditions.

3-Hydroxybutyric Acid↗

[Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins].

Bacillus megaterium accumulates poly-(3-hydroxybutyrate) (PHB) as a reserve material in intracellular granules. In this work we described a method for the preparation of PHB granules from B. megaterium PV447 and the analysis by polyacrylamide gel electrophoresis of the associated proteins. By comparison with another species a function is proposed for some of these proteins.

Acyltransferases↗

[Oxidation-reduction potentials of chlorophyll pigments in photosynthesizing organisms on different levels of evolutionary development].

According to polarographically measured EOred of bacteriochlorophyll "a", chlorobium chlorophyll "660", "b" and "a" chlorophylls (-0.67 V, -0.73 V, -0.79 V, -0.86 V-n.h.e.) and literature data about Eoox of these pigments (+/-0.62 V, + 0.62 V, + 0.78 V, +0.77 V correspondingly) it has been shown that chemical energy stored in the singular photochemical set is increased in the evolution of the photosynthetic apparatus. It has apparently resulted in the possibility of evolutionary approach to utilization of more inert and more accessible (water) electron donors and favoured further development of photosynthesizing organisms.

Bacteria↗