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At least 901 records · Page 50Linked to original sources
PubMed1995

Clinical value of an automated granulocyte elastase assay in predicting severity of acute pancreatitis.

OBJECTIVE: Quantification of circulating levels of granulocyte elastase has been shown to be a reliable method to predict severity of acute pancreatitis. The ELISA method until now available is, however, not easily applicable to the clinical routine. MATERIAL AND METHODS: In the present study we analyzed, retrospectively the clinical usefulness of an automated granulocyte elastase assay, the immunoactivation (IMAC) procedure, in predicting the course of acute pancreatitis, and we compared it with the ELISA method. PATIENTS: Plasma samples from 39 patients with acute pancreatitis, 18 with mild episodes and 21 with severe disease, were analyzed for granulocyte elastase concentration by both automated and manual ELISA procedures, on admission and on days 1, 2, 3, 5, 7 and 10. RESULTS: Automated elastase values did not differ statistically (Mann-Whitney test) from manual granuloma elastase values obtained by ELISA. Higher plasma elastase concentration was invariably found in severe pancreatitis when compared to mild cases. The optimal discriminating (severe vs mild illness) cutoff values were 200 micrograms/L on admission and 250 micrograms/L at 24 hours. Both assays showed similar prognostic reliability upper 90 per cent. CONCLUSIONS: Automated procedures enable faster and simpler granulocyte elastase determinations and exploit the benefits of this early and efficient prognostic marker.

Acute Disease↗
PubMed1994

DNA sequencing: modular primers for automated walking.

Here we describe our progress in the development of the technology of DNA sequencing by primer walking based on the modular primer approach, which eliminates the primer synthesis bottleneck. Modular primers are assembled from 5-mers, 6-mers, or 7-mers selected from a presynthesized library of as few as 1000 oligonucleotides. This technology is expected to reduce the time per walk by a factor of 20 to 50, and the cost of DNA sequencing by a factor of 5 to 15. Both time and expense will be saved not only on the primer synthesis per se but, more importantly, as a result of the closed-end automation of the complete cycle of walking sequencing, where no human intervention will be required between the walks. Such closed-end automation has until now been precluded due to the need to synthesize a new primer for each walk. As a part of the closed cycle automation development, this report deals with modular primers used for sequencing with fluorescent dye terminators. The success rate and the quality of automated sequencing with modular primers are found similar to those with the conventional 17-20-base-long primers. One of the advantages of the described technique is simple purification without any proteins that need to be removed.

Base Sequence↗
PubMed1994

Clinical laboratory automation: concepts and designs.

Compared with other industries, automation in the healthcare arena has been slow to evolve. Changes in reimbursement for services provided are forcing hospitals and other healthcare providers to look for more cost effective mechanisms to provide all forms of health related services. Clinical laboratory services are essential to the support of hospital operations and most clinic operations. The current paradigm for clinical laboratory operations is based upon a mix of both batch and random access testing and is highly dependent upon clinical laboratory personnel. Changes in the paradigm for clinical laboratory operations may result in a significant cost savings when fully implemented while maintaining a high level of quality and service for the patients. Several different laboratory organization structures are discussed. The Nebraska automation project is described including the development of a modular conveyor system, the use of automated guided vehicles, software, and network architectures. Future directions for the development of clinical laboratory operations including the creation of a "docking device" to link automation systems to instruments is proposed.

Clinical Laboratory Information Systems↗
PubMed1994

Automated electrophysiologic hearing testing using a threshold-seeking algorithm.

The efficacy of utilizing an automated algorithm to identify auditory brainstem responses (ABR) was studied. A microcomputer-based threshold-seeking algorithm utilizing click-evoked ABR was developed to determine evoked-response thresholds for automated hearing screening. The software consists of an evoked-response recognizer unit, which determines the presence or absence of a response, and a threshold-tracking unit, which controls the click intensity in order to track the threshold. The response recognizer is based upon correlation methods. Threshold tracking is accomplished using a Parameter Estimation by Sequential Testing (PEST) procedure, which is commonly used to study psychophysical properties of the auditory system. Sound level is automatically adjusted, based on the results of the recognizer and the threshold tracker. Test results were generally obtained in less than 15 minutes per ear. The results of the automated procedure correlate very highly with expert judgments of ABR threshold and show good test-retest reliability, suggesting that automated procedures are viable alternatives to traditional testing methods.

Adolescent↗
PubMed1993

Prediction of postoperative visual acuity in patients with total glaucomatous cupping using the Potential Acuity Meter and automated perimetry.

We evaluated the effectiveness of using the Potential Acuity Meter (PAM) and automated perimetry to predict postoperative vision in 30 advanced glaucoma patients following combined cataract extraction and trabeculectomy. Using the binomial distribution at alpha = 0.05, PAM and automated perimetry individually were significantly useful in predicting postoperative vision of 20/40 or better. Automated perimetry, but not PAM, was useful in predicting worse than 20/40 vision. Used together, automated perimetry and PAM testing were significantly useful in predicting vision worse or better than 20/40.

Aged↗
PubMed1993

The Bergmann Foot Scanner for automated orthotic fabrication.

The word automation sometimes can be misleading. I believe that some feel that automation of orthotic manufacturing results in a system in which orthotics are mass produced. Still others believe that the main reason for automation is to make orthotics quicker and cheaper and not individualized. As far as the author's laboratory is concerned, nothing could be further from the truth. We think of automation as the use of computer technology to make orthotics that are more accurate and more specialized as to the doctor's specific requirements. This is a method that takes the expertise of the podiatrist and the laboratory technician and gives them a tool that can be used to achieve the perfection in orthotic fabrication they are trying to attain. In testing out new types of cast correction designs, computer-designed images will give the repeatability needed in scientific research not found with manually applied corrections. The optical scanner has given us more than just a means of sending a cast to the laboratory quickly. It has turned out to be a method to help explain biomechanics of the patient's foot in a clear and concise way. We also have watched it develop into a research tool that podiatry schools and others can use to verify and further biomechanical principles.

Computer-Aided Design↗
PubMed1993

Performance of a fully automated fluorometric enzyme immunoassay for serum myoglobin measurement.

An automated fluorometric enzyme immunoassay system for the determination of serum myoglobin has been recently developed. This method is based on the sandwich immunoassay and uses two mouse monoclonal antimyoglobin antibodies; the first one is complexed onto glass fiber paper and the second is conjugated to an enzyme alkaline phosphatase which reacts with the substrate 4-methylumbelliferyl phosphate to generate a fluorescent product. Using a dedicated automated apparatus the time to the first result is eight minutes, with additional values being produced at one-minute intervals (about 50 samples/hour). We compared the analytical performance of this fluorometric enzyme immunoassay with that of a RIA set up in our laboratory for the routine assay of serum myoglobin. The automated fluorometric enzyme immunoassay showed lower between-assay variability (CV = 4.7% vs 13.8%) and higher sensitivity (0.3 ng/mL vs 7.2 ng/mL) than the manual RIA. Moreover, the two immunoassays gave similar results when serum samples of normal subjects and patients with coronary artery disease with or without acute myocardial infarction (AMI) were assayed (fluorometric immunoassay = -0.7 + 0.851 RIA, r = 0.991, n = 137). In conclusion, the automated fluorometric enzyme immunoassay tested in the present study produces reliable clinical results with a rapid turnaround time and therefore can be recommended for use in the early detection of AMI in a laboratory of Coronary Care Unit.

Evaluation Studies as Topic↗
PubMed1993

Sequence length and error analysis of Sequenase and automated Taq cycle sequencing methods.

We have examined DNA sequence error as a function of length using both a manual method of performing reactions with Sequenase and an automated Taq cycle sequencing method. DNA fragments from both methods were separated and analyzed on a sequencer. To determine the sequence of a cosmid insert (35.3 kb), 379 sequences were obtained from a manual Sequenase method, and 354 sequences were obtained from a Taq cycle sequencing method as performed on an automated robotic workstation and sequenced on an automated fluorescent sequencer. A highly redundant consensus of these sequences was obtained and aligned with the individual sequences to determine sequence error over the length of each sequence. The results of this study indicate that error is about 1% per position over the first 350 nucleotides, but increases thereafter to about 17% at 500 nucleotides. This pattern of accuracy was nearly equivalent for manual Sequenase methods and automated Taq cycle sequencing methods. The potential of these methods in large-scale DNA sequencing projects is discussed.

Cloning, Molecular↗
PubMedDate not supplied

Automated on-line quantification of left ventricular ejection fraction by echo.

An automated border detection system based on the acoustic back scatter property of ultrasound has been recently developed. This provides beat-to-beat values for the left ventricular ejection fraction (EF). This study assesses the reliability of this method when compared with the angiocardiographic EF, and manual 2D-echo-derived EF based on the modified Simpson method. Angiocardiographically-derived EF was used as the gold standard. In a total of 49 patients the angiocardiographic EF was found to be 64.49 +/- 15%. It showed excellent correlation with the automated border detection-derived EF (r = 0.91). The automated border detection-derived EF also showed excellent correlation with the manual 2D-echo method (r = 0.9). In addition, automated border detection requires less time than the manually drawn method (40.9 +/- 7.7 sec versus 58.6 +/- 7.5 sec; p < 0.001). We conclude that the acoustic quantification of the EF correlates well with the angiocardiographically-derived EF and is less time-consuming.

Echocardiography↗
PubMed1995

Synthesis and purification in a single column on a high-throughput automated oligonucleotide production system.

Oligonucleotides are synthesized and purified in a single column filled with a mixture of nucleoside-loaded and underivatized, high-cross link, non-swelling polystryene beads. A new oligonucleotide production system has been developed to completely automate-the entire process of sequence entry, phosphoramidite synthesis, cleavage, deprotection, purification, quantitation, and sample collection. Up to 48 primer-length, high-purity oligonucleotides can be produced in a 24 hour period of unattended automation on the ABI 3948 DNA Synthesizer. Synthesis, cleavage, and purification occur within the unique OneStepTM column. Analyses by MicroGel capillary electrophoresis, anion-exchange HPLC, PAGE, and enzymatic digestion/HPLC routinely exhibit > 90% product purity. Stringent PCR, automated fluorescent sequencing, and genetic analysis experiments show the positive effects of the total automation of purified oligonucleotide production.

Base Sequence↗
PubMed1977

Sensitivity of automated red cell antibody detection.

The principle of detecting red cell antibodies by automated technique using the two-channel Technicon Auto-Analyser with some modification is described. The sensitivity of automated methods in detecting antibodies of different specificities was determined. The study was carried out with 76 diagnostic human sera produced by the Institute of Hematology, Transfusion Centers and commercial firms: anti-RhD, -C, -E, -CW, -c, -e, anti-K, -k, Kpa, anti-Fya, -Fyb, anti-Jka, -Jkb, anti-M, -S, -s, anti-P1, anti-Lua, anti-Lea, -Leb, anti-H, -A1, anti-I, anti-Lua and anti-Vel. A majority of the antibodies were detected by both automated methods with greater sensitivity than by manual methods. Greatest sensitivity was observed in the detection of anti-S, -Vel and antibodies of the Rh system. Anti-Leb and anti A1 antibodies were not detected. The studies are the basis for introduction of the automated technique into routine use for antibodies in blood donors and recipients.

Antibody Specificity↗
PubMedDate not supplied

Robotic automated analysis of foods for aflatoxin.

Immunoaffinity column-based sample preparation procedures for determination of aflatoxins B1, B2, G1, and G2 in several food matrixes and aflatoxin M1 in milk have been automated by using flexible automation, or robotics. Components used to assemble the system were purchased commercially or developed and built in-house. A liquid-level sensor developed in-house to assist elution of the immunoaffinity column is described. After immunoaffinity column cleanup, aflatoxins are separated by reversed-phase liquid chromatography and determined by fluorescence without derivatization. Mean recoveries of aflatoxins B1, B2, and G1 added to corn and nuts at 9-36 ng/g total aflatoxins were > 85% (coefficient of variation [CV] = 16%). Recoveries of aflatoxin G2 averaged 50% (CV = 28%). Recoveries of aflatoxin M1 added to milk at 0.12-0.50 ng/mL averaged 78% (CV = 19%). The ability of the automated system to reproduce its results is demonstrated by the fact that the CV of replicate assays is generally better than 10%. Comparability between the automated procedure and the AOAC official method is demonstrated.

Aflatoxins↗
PubMed1997

[US-guided core biopsy of the breast with an automated biopsy gun: comparison with an aspiration core needle device].

To evaluate the efficacy of an automated Tru-cut type of biopsy gun in US-guided breast biopsy, we performed 162 breast biopsies in 148 patients using an 18 gauge short-throw (1.1cm excursion) Tru-cut-type biopsy gun (Ace-cut needle). The results of the series were compared with another series of aspiration core biopsies, which were performed on 113 breast lesions in 112 patients using an 18 gauge Sure-cut needle. The results of the two series were correlated with the diagnoses made at surgery or clinical follow-up. Sensitivity, specificity, and accuracy for the automated biopsy gun were 89.2%, 94.9%, and 92.7%, and for the Sure-cut needle, 75.0%, 78.6%, and 77.5%, respectively (p < 0.01 for specificity and accuracy, by chi 2 test). There were no serious complications. The diagnostic surgical biopsy to operated cancer ratios in 1991 (without core biopsy), 1993 (with aspiration core biopsy), and 1995 (with automated core biopsy) were 2.9 (46/10), 2.0 (30/17), and 0.5 (12/24), respectively. We concluded that US-guided breast biopsy with an automated biopsy gun is a safe and highly accurate method, and could replace the diagnostic surgical biopsy.

Biopsy, Needle↗
PubMed1997

Home self-monitoring of blood pressure: is fully automated oscillometric technique as good as conventional stethoscopic technique?

Home blood pressure (HBP) measurement is becoming increasingly popular as an additional source of information for the practicing physician. Whether HBP measured with a fully automated oscillometric device (oHBP) is more reliable than HBP measured with an aneroid sphygmomanometer and a stethoscope (sHBP) remains unclear. We compared sHBP with oHBP using as a reference method daytime ambulatory blood pressure (ABP), as this is believed to be a better index of an individual's overall level of pressure. Forty-six hypertensive patients measuring HBP with aneroid devices were retrained by a standard 30 min protocol that included training in the technique of measurement, checking patients' devices, and testing patients' performance in stethoscopic measurement. Patients were randomized to measure for 2 weeks either sHBP using their own calibrated aneroid devices or oHBP using a validated fully automated oscillometric device (Omron HEM-705CP). Then 24 h ABP monitoring was performed (SpaceLabs 90207) and patients crossed over for a second 2 week period by using the alternative HBP measurement technique. Mean sHBP was not different from mean oHBP, and there was a close correlation between them (r = 0.82/0.76 for systolic/diastolic BP, P < .001). Daytime ABP was not different from oHBP or sHBP and was closely related to both of them (oHBP, r = 0.59/0.72 systolic/diastolic BP, P < .001; sHBP, 0.50/0.65, P < .001). Age was significantly related with diastolic ABP-sHBP difference (r = 0.33, P < .05). These results suggest that HBP measured with validated fully automated oscillometric devices is equally reliable in predicting average ABP as that measured with calibrated aneroid sphygmomanometers used by very carefully trained patients. In clinical practice, HBP monitoring by using reliable automated devices is probably more feasible than to achieve a high standard of stethoscopic HBP measuring technique.

Adult↗
PubMedDate not supplied

Use of automated reminders for tuberculin skin test return.

INTRODUCTION: This study assessed the impact of automated telephone reminders on tuberculin skin test returns. METHODS: A total of 701 English-speaking and Spanish-speaking patients of a public health immunization program were randomly assigned to an intervention or a control group. Those in the intervention group received an automated telephone reminder to return for the reading of their skin test. RESULTS: Automated telephone reminders significantly reduced return failures 53% (from 14% to 7%) when the scheduled interval between test administration and reading was three days, but had no impact for a two-day interval. Effectiveness of reminders did not differ significantly by patient age, gender, or language (English versus Spanish). CONCLUSIONS: Results suggest the value of automated reminder calls for intra-appointment intervals as short as three days.

Adolescent↗
PubMedDate not supplied

Preparing to automate the case management process.

In this article, the authors discuss case management automation and the preparation that an organization should be making to install such a system successfully. They begin with a discussion of the goals of case management automation, and then review the steps that all facilities with case management programs can undertake to standardize their process, structure their organization, and strategically plan for case management automation. The current status of case management automation efforts also is discussed. The authors conclude by describing the efforts of one organization to develop an integrated case management documentation system.

Case Management↗
PubMed1998

Use of new automated biopsy device for percutaneous testicular biopsy. A preliminary clinical experience.

Testicular biopsy is indicated in the azoospermic patients to determine if the cause of azoospermia is ductal obstruction or testicular pathology and in certain patients who have severe oligozoospermia. The technique traditionally used is an open testicular biopsy. New Automated Biopsy Device has been used successfully for renal diseases. This instrument is now used by the urologist for transrectal prostate biopsy. The aim of this study was to evaluate whether percutaneous testicular needle biopsy with new automated device can replace open testis biopsy or not. 90 testes of 48 patients between 22 and 43 years olf underwent percutaneous testicular biopsy with a new 18-gauge automated biopsy device. Adequate samples were recovered from 90% cases (average 24.77 tubule sections per testis). When needle biopsy and open biopsy samples were compared in 30 testes of 15 patients, complete agreement was obtained in 93%, whereas slight differences in 7%. These results show that percutaneous needle biopsy with new automated systems is cost-effective, quick, safe, repeatable, relatively painless and effective way to obtain largest possible cores through full cut technique. No serious complications were encountered during or after the procedure. The diagnosis were found to be comparable to those obtained by open biopsy. For these reasons, it is concluded that this new technique can replace the open biopsy.

Adult↗
PubMed1998

Rapid sequencing of the p53 gene with a new automated DNA sequencer.

p53 is the most commonly mutated gene in human cancers. Approximately 90% of the p53 gene mutations are localized between domains encoding exons 5 to 8. Sequencing methods currently available are tedious and time-consuming and are not suitable for routine laboratory testing. In an effort to identify a simple and rapid sequencing method, we analyzed 16 preselected breast tumors and 18 preselected ovarian tumors, using a newly developed automated DNA sequencer. p53 gene mutations had been previously identified in these tumors, using a conventional automated sequencing procedure. Exons 5 to 8 were amplified by PCR, and the PCR products were subsequently subjected to cycle sequencing with the Sanger chain termination method, using Cy5.5-labeled primers. The sequencing mixture was then resolved on a newly developed automated DNA sequencer that can sequence approximately 300 bases of DNA in 30 min. Of these 16 breast tumors, two had mutations in exon 5, four in exon 6, three in exon 7, and three in exon 8. Of the 18 ovarian tumors, two had mutations in exon 5, five in exon 6, two in exon 7, and three in exon 8. In all cases, we identified the same mutations by both the new and the conventional sequencing procedures. Most mutations affected an arginine codon. These data demonstrate that the new method has the capability to provide accurate sequencing information in a fraction of the time and labor in comparison with current automated sequencing techniques. When such procedures are used, DNA sequencing may become a routine tool for identifying clinically important mutations for diagnosis and prognosis of patients with genetic, malignant, infectious, and other diseases.

Autoanalysis↗