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At least 91 records · Page 5Linked to original sources
PubMed1994

[Clinical application of gene technology to monitor bone marrow transplantation].

We assessed the origin of peripheral blood cells and bone marrow cells obtained from 15 patients after allogeneic bone marrow transplantation (allo BMT) by sensitive two-step polymerase chain reaction (PCR) amplification of MCT118, a variable number of tandem repeats regions (VNTR), that can be used to detect the DNA pattern of a minor cell population of only 1% without using radioisotopes. Mixed chimerism(MC) was detected in the haematopoietic cells of 3 patients. Two patients developed relapse of leukaemia after the detection of MC and one patient died of bone marrow hypoplasia 7 months after BMT. These findings indicate the clinical usefulness of this method to monitor patients with MC. Also, we analyzed cytokine gene expression in peripheral blood mononuclear cells during the development of graft-versus-host disease (GVHD) in patients who underwent allo BMT using a semiquantitative reverse-transcriptase polymerase chain reaction (RT-PCR). The expression of interleukin(IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha mRNA was increased during the development of GVHD and the degree of this increment depended on the severity of the disease. These findings suggest that IL-1 beta, IL-6, and TNF-alpha produced by peripheral blood mononuclear cells play an important role in the development of GVHD. Therefore, analysis of MC and cytokine mRNA expression using the PCR technique after allogeneic bone marrow transplantation provide important information for treatment and monitoring of marrow transplant patients.

Adolescent↗
PubMed1996

Reporter gene technology to assess activity of antimycobacterial agents in macrophages.

Reporter strains of Mycobacterium tuberculosis and Mycobacterium bovis BCG endogenously expressing firefly luciferase were used in bioluminescence assays to evaluate the activities of isoniazid and rifampin against mycobacteria sequestered in human macrophages. This methodology allowed the efficacy of antibiotics against intracellular mycobacteria to be assessed without the labor-intensive procedures and protracted incubation requirements associated with conventional CFU determinations.

Anti-Bacterial Agents↗
PubMed1994

[Application of gene technology to clinical pathology].

Recently striking progress has been made in molecular biology. Among the newly developed techniques, PCR (polymerase chain reaction) is making an epoch in DNA diagnosis in the field of clinical pathology and laboratory medicine. In this symposium, we discussed the available PCR methods in DNA diagnosis and also the future prospect of PCR method as a routine laboratory procedure. First, Dr. Kawabata reviewed the PCR technique and analytical procedures of PCR products. Next five symposium presented the data on DNA diagnosis for particular diseases using the PCR method. Dr. Hasebe presented the HCV genotyping data from the patients. Dr. Hirose showed us the difference in accuracy in DNA typing of Chlamydia trachomatis antigen between the DNA probe method and PCR method. He also introduced the newly developed LCR method. Dr. Kondo reviewed the methods of DNA diagnosis for malignant lymphoma. Dr. Yaginuma presented the analyzed data of tumor suppressor gene p53 in some gynecological tumors. Dr. Azuma presented a case of chronic granulomatosis with a point mutation on the gp91-phox gene which had been revealed by RT-PCR. Next Dr. Yagihashi introduced the protocol for DNA typing of the HLA class II. Finally, Dr. Tanaka presented the availability of the PCR method for the convalescent screening of bone marrow transplantation. We hope this symposium is fruitful and motivating for all staffs of laboratory medicine and pathology. In closing my remarks, I express our appreciation to all symposists as well as Prof. Hisami Ikeda, president of the 27th general meeting of the Hokkaido branch, the Society of Clinical Pathology, planned this symposium.

Genetic Techniques↗
PubMed1986

[Viral vaccines produced by gene technology and synthesis].

The gradual evolution of vaccine development from Jenner's smallpox vaccine to recombinant genetechnology has provided the basis for prophylactic control of a lot of important infectious diseases of humans and animals. Modern biotechnology has accomplished antigen identification and isolation of antigen determinants as well as the preparation of proteins and synthetic peptides. The application of such techniques has failed, however, to develop a better vaccine in case of FMD-Virus. Present efforts are directed on the improvement of antigen presentation and immunogenicity of such protein- and peptide vaccines.

Amino Acid Sequence↗
PubMed1989

Receptor binding and biologic activity of biosynthetic human insulin and mini-proinsulin produced by recombinant gene technology.

Human insulin and its precursor, mini-proinsulin, made with a new biosynthetic method, were tested for their receptor binding, biologic action, and antibody binding ability. The structure of mini-proinsulin is similar to that of proinsulin with a shortened C-peptide, B(1-29)-Ala-Ala-Lys-A(1-21) insulin. The ability of biosynthetic human insulin to bind to receptors, to stimulate 2-deoxyglucose uptake in isolated adipocytes, and to bind to insulin antibody was comparable to that of semisynthetic human insulin. The ability of mini-proinsulin to bind to insulin receptors and to stimulate 2-deoxyglucose uptake in adipocytes was 0.5 and 0.2% that of human insulin, whereas the corresponding abilities of proinsulin were 5 and 3%, respectively. Despite having less receptor binding and biologic activity, mini-proinsulin demonstrated higher affinity for the insulin antibody than did proinsulin. These results suggest that biosynthetic human insulin behaves similarly to semisynthetic human insulin in its receptor binding and biologic activity, and that the shortened C-peptide region reduces receptor binding by fixing or covering the N-terminal region of the A chain, which is important for receptor binding.

Adipose Tissue↗
PubMed1989

[Gene technology and biological safety].

A "European Biosafety Workshop on the Uncontained Use of Modified Organisms in the Environment" was held in Berlin on 27-30 November 1988. The author's concluding remarks are given here in a free German translation. The article briefly summarizes the overall conditions for a general evaluation of potential benefits and risks that may be associated with the use of genetically modified organisms.

Accident Prevention↗
PubMed2006

Comparative analysis of the risk-handling procedures for gene technology applications in medical and plant science.

In this paper we analyse how the risks associated with research on transgenic plants are regulated in Sweden. The paper outlines the way in which pilot projects in the plant sciences are overseen in Sweden, and discusses the international and national background to the current regulatory system. The historical, and hitherto unexplored, reasons for the evolution of current administrative and legislative procedures in plant science are of particular interest. Specifically, we discuss similarities and differences in the regulation of medicine and plant science, and we examine the tendency towards dichotomizing risk--focusing on social/ethical risks in medicine and biological risks in plant science. The context of this article is the Synpraxia research project, an inter-disciplinary program combining expertise in sciences and the humanities.

Biomedical Technology↗
PubMed2002

Visualizing selective neural pathways with WGA transgene: combination of neuroanatomy with gene technology.

Functional logic employed by the nervous system for information processing resides mainly in the wiring patterns among specific types of neurons. Therefore, detailed knowledge on neuronal networks is essential for understanding a wide range of brain functions. A powerful and long-awaited method for analyzing the neuronal connectivity patterns would be to deliver tracers selectively to specific types of neurons and at the same time to label transsynaptically their axonal target neurons. For this purpose, we took advantage of a unique property of plant lectin, wheat germ agglutinin (WGA), which has been used as a transsynaptic tracer in classical neuroanatomical studies. We developed a novel genetic strategy that employs WGA cDNA as a transgene, for the visualization of selective and functional neural pathways in the nervous system. In this article, I will introduce several examples of neural pathways visualized with the WGA transgene and discuss about its further refinement and applications.

Animals↗