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Results for “biostimulation”

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PubMed2001

Signal transduction pathways involved in low intensity He-Ne laser-induced respiratory burst in bovine neutrophils: a potential mechanism of low intensity laser biostimulation.

BACKGROUND AND OBJECTIVE: Low intensity He-Ne laser irradiation has been reported to induce respiratory burst of neutrophils for a long time, but the mechanism remains obscure. We speculated that it is mediated by some signal transduction pathways. STUDY DESIGN/MATERIALS AND METHODS: The protein tyrosine kinases (PTKs) inhibitor, genistein, the phospholipase C (PLC) inhibitor, U-73122, and the protein kinase C (PKC) inhibitor, calphostin C, were used to probe signal transduction pathways of respiratory burst of bovine neutrophils which were induced by He-Ne laser at a dose of 300 J/m(2), respectively. RESULTS: The inhibitor of PTKs can completely inhibit the He-Ne laser-induced respiratory burst of neutrophils. PLC and PKC inhibitors can obviously reduce it, but not fully inhibit it. CONCLUSION: These results suggest that PTKs play a key role in the He-Ne laser-induced respiratory burst of neutrophils and [PTK-PLC-PKC-NADPH oxidase] signal transduction pathways may be involved in this process.

Animals↗
PubMed2001

Monitoring the Size and Metabolic Activity of the Bacterial Community during Biostimulation of Fuel-Contaminated Soil using Competitive PCR and RT-PCR.

Efforts to understand and improve soil bioremediation are limited by our ability to determine how treatment variables affect microbial communities. A method was developed to monitor the density and metabolic activity of the total bacterial community in soil. This method was used to monitor the bacterial community in microcosms of Arctic soil after addition of N plus P to stimulate biodegradation of hydrocarbon contaminants. During 29 days of incubation, the total petroleum hydrocarbon level in the soil was reduced from 850 to 360 mg/g of soil. DNA and RNA were extracted from soil using a bead beating method, purified by ammonium acetate precipitation, and assayed by competitive PCR and RT-PCR assays with universal bacterial primers. The copy number of 16S rDNA in the soil microbial community was relatively stable and ranged from 1.7 x 109 to 4.5 x 109/g of soil throughout the incubation. The copy number of 16S rRNA changed substantially and ranged from 5.6 x 1010 to 1.0 x 1012/g of soil. The rRNA:rDNA ratio was highest during the phase of fastest hydrocarbon biodegradation. These results suggest that the treatment to stimulate hydrocarbon biodegradation did not substantially change the density of the bacterial community but did transiently increase its overall metabolic activity.

Journal Article↗
PubMed2003

Biostimulation of natural microbial assemblages in oil-amended vegetated and desert sub-Antarctic soils.

A field study was initiated in December 2000 in two selected soils of The Grande Terre (Kerguelen Archipelago) with the objective of determining the long-term effects of fertilizer addition on the biodegradation rate and the toxicity of oil residues under severe sub-Antarctic conditions. Two soils were selected. The first site supports an abundant vegetal cover; the second one was desert soil, devoid of plant material. These two soils were located in the vicinity of the permanent station of Port-aux-Français (69 degrees 42'E; 49 degrees 19'S). A series of five experimental plots (0.75 x 0.75 m) were settled firmly into each of the studied soils. Each plot received 500 mL of diesel or Arabian light crude oil, and some of them were treated with a bioremediation agent: slow-release fertilizer Inipol EAP-22 (Elf Atochem). All the plots were sampled on a regular basis over a 1 year period. Heterotrophic and hydrocarbon-degrading microorganisms increased by two orders of magnitude during the first month of the experimentation in all treated enclosures, but differences appeared between the different plots. The microbial response was improved by bioremediation treatments. However, fertilizer addition had a greater impact on the desert soil when compared to the vegetated one. All chemical indices show a reduction of alkanes and light aromatics. Toxicity results show a high variability between treatments and environmental conditions. As a conclusion, it is clear that the microbial response was rapid and efficient in spite of the severe weather conditions, and the rate of degradation was improved by bioremediation treatments. However, after 1 year of treatment, the signal of a relatively high toxicity of oiled residues remained present in the two studied soils.

Acridine Orange↗
PubMed1990

Low power laser biostimulation of chronic oro-facial pain. A double-blind placebo controlled cross-over study in 40 patients.

The efficacy of low power laser stimulation in the treatment of chronic oro-facial pain conditions was investigated in a double-blind placebo controlled modified cross-over study in 40 patients. The laser was an invisible infrared (IR) diode laser with an emission at 904 nanometer (nm). Treatment effect was evaluated by means of VAS-scales and global assessment of pain. Outcome of treatment was correlated to changes in urinary excretion of 5-hydroxyindoleacetic acid (5-HIAA). The clinical impression was that placebo was superior to laser stimulation. No statistically significant difference between the analgesic effect of the laser and placebo irradiation was found on VAS-scales. A significant (P = 0.05) increase in 5-HIAA excretion was found in the placebo group. It is concluded that the possibility of a substantial placebo response should be taken into consideration using 904 nm (IR) lasers for pain treatment in patients with this type of chronic oro-facial pain.

Adult↗
PubMed2007

Effects of temperature and biostimulation on oil-degrading microbial communities in temperate estuarine waters.

Improved strategies for oil-spill remediation will follow a better understanding of the nature, activities and regulating parameters of petroleum hydrocarbon-degrading microbial communities in temperate marine environments. The addition of crude oil to estuarine water resulted in an immediate change in bacterial community structure, increased abundance of hydrocarbon-degrading microorganisms and a rapid rate of oil degradation, suggesting the presence of a pre-adapted oil-degrading microbial community and sufficient supply of nutrients. Relatively rapid degradation was found at 4 degrees C, the lowest temperature tested; and it was temperature rather than nutrient addition that most influenced the community structure. A detailed phylogenetic analysis of oil-degrading microcosms showed that known hydrocarbonoclastic organisms like Thalassolituus and Cycloclasticus, as well as proposed oil degraders like Roseobacter, were present at both 4 degrees C and 20 degrees C, demonstrating the thermo-versatility of such organisms. Clones related to Oleispira antarctica (98% 16S rRNA similarity), a psychrophilic alkane degrader, were dominant in the 4 degrees C oil-degrading community, whereas other clones constituting a different clade and showing 94% similarity 16S rRNA with O. antarctica were found in situ. These findings demonstrate the potential for intrinsic bioremediation throughout the course of the year in temperate estuarine waters, and highlight the importance of both versatile psychrotolerant and specialized psychrophilic hydrocarbon-degrading microbes in effecting this process at low temperatures.

Bacteria↗
PubMed1986

Biostimulation.

Explore the source record for details and available documents.

Electric Stimulation Therapy↗
PubMed1986

Cerebral (cortical) biostimulation.

Presently, stimulation of the surface of the brain in order to provide artificial vision or hearing for the blind and deaf is in its infant stages. Those observations which are accumulating now have as their aim the investigation of the feasibility of such prosthetic devices. Whether such devices will ever be of real therapeutic benefit remains uncertain and can only be determined by this type of very labor-intensive, expensive and interdisciplinary investigation. When one considers, on the other hand, that there are between one-quarter and one-half million individuals in the United States and Canada who are profoundly deaf and a similar number who are legally blind, the value of the pursuit of such investigation requires little defense.

Cerebral Cortex↗
PubMed1993

Attempted biostimulation of division in Saccharomyces cerevisiae using red coherent light.

Replicate cultures of the yeast Saccharomyces cerevisiae were irradiated with 632.8 nm coherent light from He-Ne lasers at irradiances of 6.5 x 10(15) and 1.0 x 10(16) photons s-1 cm-2. Irradiation periods ranged from 0 to 652 min, and cultures were grown until well into the exponential phase. Unirradiated control cultures were grown alongside the irradiated cultures under otherwise identical conditions. The extents of growth in the control and irradiated cultures were compared spectrophotometrically at the end of each experiment. Contrary to the expectations of Karu et al. (e.g. Karu, 1988, Lasers Life Sci. 2, 53-74) no growth enhancement was found in the irradiated cultures, but a mild inhibitory effect was observed.

Light↗
PubMed2004

Molecular characterization of a dechlorinating community resulting from in situ biostimulation in a trichloroethene-contaminated deep, fractured basalt aquifer and comparison to a derivative laboratory culture.

Sodium lactate additions to a trichloroethene (TCE) residual source area in deep, fractured basalt at a U.S. Department of Energy site have resulted in the enrichment of the indigenous microbial community, the complete dechlorination of nearly all aqueous-phase TCE to ethene, and the continued depletion of the residual source since 1999. The bacterial and archaeal consortia in groundwater obtained from the residual source were assessed by using PCR-amplified 16S rRNA genes. A clone library of bacterial amplicons was predominated by those from members of the class Clostridia (57 of 93 clones), of which a phylotype most similar to that of the homoacetogen Acetobacterium sp. strain HAAP-1 was most abundant (32 of 93 clones). The remaining Bacteria consisted of phylotypes affiliated with Sphingobacteria, Bacteroides, Spirochaetes, Mollicutes, and Proteobacteria and candidate divisions OP11 and OP3. The two proteobacterial phylotypes were most similar to those of the known dechlorinators Trichlorobacter thiogenes and Sulfurospirillum multivorans. Although not represented by the bacterial clones generated with broad-specificity bacterial primers, a Dehalococcoides-like phylotype was identified with genus-specific primers. Only four distinct phylotypes were detected in the groundwater archaeal library, including predominantly a clone affiliated with the strictly acetoclastic methanogen Methanosaeta concilii (24 of 43 clones). A mixed culture that completely dechlorinates TCE to ethene was enriched from this groundwater, and both communities were characterized by terminal restriction fragment length polymorphism (T-RFLP). According to T-RFLP, the laboratory enrichment community was less diverse overall than the groundwater community, with 22 unique phylotypes as opposed to 43 and a higher percentage of Clostridia, including the Acetobacterium population. Bioreactor archaeal structure was very similar to that of the groundwater community, suggesting that methane is generated primarily via the acetoclastic pathway, using acetate generated by lactate fermentation and acetogenesis in both systems.

Archaea↗
PubMed1990

[Biostimulation of wound healing in rats by combined soft and middle power lasers].

For some time now, the combined soft- and middle-power laser has been used for treating wounds and various skin diseases, but its effect has not yet been confirmed in experiments on animals. For this reason, a controlled test on the effect of this laser on wound healing in rats was conducted. In 16 rats, 3-cm long skin incisions were made on the wall of the abdomen and then sutured using standard surgical technique with 5 stitches. Eight rats were then treated with laser light, the other 8 receiving a control treatment. The wounds were photographed on the 1st, 2nd, 3rd, 5th, 8th and 15th postoperative day. The photographs were shown to 10 doctors who rated the wounds from 1 to 4 on the basis of the criteria inflammation and adaptation. While the wounds of the laser group were not judged to be significantly better than those of the control group in terms of inflammation, adaptation was judged to be significantly better in the laser-treated group. This effect was particularly evident at the beginning of treatment (3rd day: average rating of laser group: 1.68; S = 0.32; control group: 2.41; S = 0.53; analysis of variance parameters for repeated measurements: p = 0.027 less than 0.05). These results demonstrate that in all probability the combined soft- and middle-power laser promotes the closing of wounds in rats, especially in the first postoperative days, while there is no positive effect on inflammation.

Animals↗