The correspondence between the orthopantomographic and the clinical apperance of an erupting tooth (first molar).
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The aim of this study was to determine the eruption times of permanent teeth, excluding third molars, in a defined area population of a country district in Lower Saxony. The authors investigated 2495 oral findings of 664 patients (male 315, female 349) in a longitudinal study. The minimum age was 3.00 years and the maximum age 24.00 years. The dental findings were collected over a period of about 20 years (1980-2002). The oral findings per child/adolescent were assessed between one and 16 times. The eruption times of teeth in females are earlier than those for the same teeth in males. Further, the permanent dentition in females is completed earlier than in males. The tooth eruption occurs symmetrically in both jaws. The comparison of both jaws revealed a slightly advanced eruption of the lower jaw teeth for both sexes. There is a noteworthy change in the eruption sequence of the teeth. In contrast to other reports we determined that the eruption of the canine proceeds the eruption of the second premolar. We found no acceleration of the dentition when compared to other reports and confirmed the rules of tooth eruption in man. Oral examination of teeth is a simple tool to calculate tooth eruption intervals. This first investigation on a population of Lower Saxony revealed a change in the eruption sequence of permanent teeth. The findings are relevant for dental treatment planning and should be reconfirmed at certain intervals.
A case of a mesiodens in the vault of the palate of a skull estimated to be approximately 13,000 years old is presented. The currently cited theories of tooth eruption were considered, and it was concluded that only the newly formulated BVT (blood vessel thrust) theory of tooth eruption can account for the migration of this mesiodens into that position.
A survey was carried out on the timing of deciduous tooth eruption based upon 3600 Caucasian children in three heterogeneous samples from Birmingham, Sheffield and Gloucester and a small more or less homogeneous sample from Biddulph (North Staffordshire). These data were compared with the eruption times derived from a sample of 600 British Negro children. Generally, the deciduous teeth of Negroes erupted before those of Caucasians, although the actual eruption times in each sample exhibited a greater degree of variability than is appreciated by most dental workers.
The aim of the present paper is to determine the beginning, order, median time, termination, dynamics, continuity and intensity of the primary tooth eruption, as well as its calendar, in a sample of children from Zagreb, Croatia. The study included 1288 children. All subjects were classified in one-year age groups. In the examined group, the continuity of tooth eruption occurs from 4.2 months to 41.5 months, i.e., for a period of 37.3 months. Each particular tooth has its own dynamics of eruption (V5-V95) which on the average amounts to 14.35 months (14.92 months in boys and 13.79 months in girls). The continuity of eruption is 35.6 months in boys and 38.9 months in girls. The dynamics of eruption is 14.82 months in boys and 13.79 in girls. The termination of tooth eruption is similar for both sexes.
Previous studies from our laboratories have shown that premolar eruption in dogs depends upon the presence of the dental follicle and is independent of root or crown growth or attachment to the oral epithelium. The present study is an analysis of the cellular composition of the dental follicle and the cellular investment of the adjacent walls of the bony crypt before and during eruption of the third and fourth mandibular permanent premolars in young beagle dogs. Four premolar follicles and their adjacent bony crypts were examined at 2-week intervals over 12 weeks before and during eruption of these teeth. Tissues were removed, fixed, processed, and oriented so that each follicle and the adjacent crypt wall could be reproducibly examined in vertical and horizontal planes. Mononuclear cells with abundant cytoplasm, euchromatic nuclei, and prominent nucleoli were present in juxtavascular location in the coronal part of the dental follicle; and these cells increased in number immediately preceding and during tooth eruption in parallel with an increase in osteoclasts on the adjacent crypt wall. These data are interpreted to mean that the coronal part of the dental follicle may coordinate the alveolar bone resorption required for tooth eruption by attracting and directing to the crypt wall a population of mononuclear cells, which either become osteoclasts and/or direct osteoclastic activity during tooth eruption.
The tooth eruption patterns of two large groups of young living marmosets were compared. The eruption sequence was essentially similar and was consistent with previous studies based on smaller numbers of dead animals. At nine mo, however, one group was more advanced than the other, although their craniofacial growth patterns were similar.
An in-vitro model that simulates tooth eruption was devised to determine the possible role of periodontal ligament fibroblasts (PLF) in providing a major force for eruption. The model consisted of agarose-coated wells in multiwell tissue-culture plates to which wire mesh was attached at the rim and a slice of demineralized root was attached to the floor. When the wells were filled with a collagen lattice containing PLF, the lattice contracted and elevated the root slice. The root slice in models that were unattached at the rim was not elevated. Cultures without cells did not contract and root slices were not elevated. Elevation was inhibited by cytochalasin D and Colcemid. Histologically, cultures that had elevated showed orientated cells and collagen fibres. The results suggest that PLF may be capable of generating forces that are sufficient to produce tooth eruption.
The purpose of this study was to examine the vascular network beneath the reduced enamel epithelium (REE) and oral epithelium (OE) during tooth eruption. Using the corrosive-resin casting method, vascular resin casts were prepared from premolar and molar teeth of the dog and examined by scanning electron microscopy. The vascular network beneath the REE is arranged in two layers, an inner layer consisting of a dense capillary network and an outer layer composed primarily of arterioles and venules. Throughout tooth eruption, the configuration of the vessels of the inner layer is continually changing from a network of capillary loops to a fishnet pattern, whereas the arrangement of the vessels of the outer layer remains fairly constant. When the crown emerges into the oral cavity, leakage of resin from the vessels provides evidence of increased vascular permeability, suggesting the presence of inflammation within the marginal gingiva. When root development is nearly complete, the vascular network adjacent to the REE becomes continuous with that of the OE. The inner margin of the free gingiva receives its blood supply from this network, and the vessels of this network also give off branches which anastomose with the capillary network of the periodontal ligament.
Cleidocranial dysplasia (CCD) is an autosomal dominant human disorder that affects development of bones and teeth. The dental disorders in CCD patients include formation of supernumerary teeth, delayed tooth eruption, and lack of formation of cellular cementum in permanent teeth. This disorder involves a mutation in the osteoblast-specific transcription factor Runx2/Cbfa1, leading to haploinsufficiency of the Runx2/Cbfa1 protein. Here, we examined if Runx2/Cbfa1 heterozygous mice (with one functional allele for Runx2/Cbfa1) exhibit similar changes in tooth eruption, and dental cementum formation as in CCD patients. Heads of Runx2/Cbfa1 heterogeneous and wildtype mice aged days 16-35 postnatally were serially sectioned and stained with hematoxylin-eosin or for tartrate resistant acid phosphatase (TRAP) to identify osteoclasts. The results showed that the eruption pattern of the first and second molars in maxilla and mandible in Runx2/Cbfa1 +/- mice was the same as in wildtype animals. No clear difference in distribution or in the (estimated) number of osteoclasts was found. Cellular cement at the apical portions of the molar roots was present in both groups. The data suggests that in the mouse one allele for Runx2/Cbfa1 is sufficient for an undisturbed tooth eruption and an apparently normal formation of the periodontium.
This experiment investigated the reason for disturbances of tooth eruption through hydroxylapatite (HA) granules. HA granules of compact and porous structure were implanted in the pathway of erupting teeth in 14 five-day-old rats. The results demonstrate that the use of this material can result in tooth retention and malformed tooth crowns. Tooth crown malformation can be traced to the formation of ceramo-dentinous complexes which promote bacterial invasion of the pulp cavity, resulting in arrest of the tooth eruption process.
It has been shown that a specific inhibitor of vacuolar H(+)-ATPases, bafilomycin A1, inhibits bone resorption by isolated chicken osteoclasts by blocking the proton pump in the ruffled border membrane. We report here the effects of bafilomycin A1 on bone resorption in vivo. Using a cannulated osmotic minipump delivery system, we infused bafilomycin locally to the eruption pathway of permanent premolars of beagle dogs. We used pit formation by osteoclasts in vitro to estimate the concentrations and heat stability of bafilomycin to be used in vivo. In this model, osteoclasts were cultured on thin bone slices, in which they form pits indicative of resorption. After 2 weeks preincubation at 37 degrees C, bafilomycin concentrations of 10(-6) and 10(-7) M but not 10(-8) M completely inhibited the resorptive activity of cultured osteoclasts, and the two larger doses were chosen for use in vivo. Local delivery of 10(-6) M bafilomycin to the eruption pathway of the fourth permanent mandibular premolar during mideruption inhibited tooth eruption by blocking bone resorption as assayed by radiography, light microscopy, and scanning electron microscopy. Bafilomycin at 10(-7) M had similar but less intensive effects. Moreover, osteoclasts in the alveolar bone of crypts treated with 10(-7) M bafilomycin A1 stained very weakly for tartrate-resistant acid phosphatase. The effect of bafilomycin on bone resorption was shown to be very local, and no side effects of treatment with bafilomycin were observed in adjacent teeth or the behavior of dogs. We report here, for the first time, inhibition of tooth eruption caused by inhibited bone resorption using bafilomycin A1 in vivo.
How the dentition erupts, and what controls it, is fundamental to almost all aspects of clinical dentistry, yet the mechanisms behind this have not yet been fully elucidated. When the process continues into space that has been created through toothwear or tooth loss, problems in placing restorations can be encountered. This review examines the possible mechanisms of tooth eruption. Differences between processes in animals and humans are highlighted. The limitations of conclusions drawn from animal studies are then discussed with reference to human dental conditions. The differing forms of overeruption in humans are described and the treatment options for overerupted teeth, including prevention of the situation arising, are provided with a discussion of the quality of the evidence base behind these.
Fibroblast cell population kinetics in the developing molar periodontal ligament was investigated in 10, 12, 16 and 20 days old mice by autoradiography after the administration of [3H]thymidine. Labelled mitoses, in number per unit area, were counted over the apical zones of the sections and percentage labelled mitoses (PLM) curves were drawn. Median values for some phases durations were read off at the 0.5 level of mitotic labelling. In determining other kinetic parameters the periodontal fibroblast population was considered separately as (1) a steady state system, (2) an exponentially growing system. An attempt was made to estimate mean values for these parameters using the Gilbert Computer programme. The programme generated the original data points and the fitted curve in graphical and numerical form together with the mean values and standard errors. The fact that the Gilbert programme assumes a stationary population in its theoretical PLM curve analysis was used to establish the kinetic type of the periodontal fibroblast population. The present study has demonstrated that in the growing periodontal ligament where cell specialisation and migration occurs steady state system is the kinetic model applicable. Failure of the computed PLM curves to fit the data adequately confirmed the fibroblast migration (apico-occlusal migration) from the apical zone to other zones. A definite cytokinetic basis for the periodontal ligament fibroblast proliferation and migration was established. Accordingly, fibroblast proliferation and migration takes place by a reduction in the DNA synthesis time (T8), cell generation tie (Tc) and possibly by a similar reduction in other parameters. Maximum reduction in these parameters is associated with the peak proliferative and migratory activity in the 12 days old group which is also the time that tooth eruption takes place in the mouse. Thus fibroblast proliferation and migration are major causative factors responsible for tooth eruption. Based on these results, a mechanism for tooth eruption is proposed.
The grey lethal mouse is an osteopetrotic mutant in which there is diffuse sclerosis of the entire skeleton and the teeth remain trapped in the jaws. The condition was treated with daily subcutaneous injections of PTE beginning at birth. PTE induced molar tooth eruption in some mutants and improved root formation (without eruption) in others. However, the zone of predentine in the crown of the tooth remained abnormally wide and dentinogenesis remained irregular. Resorption of alveolar bone was adequate to permit complete tooth eruption in some animals and increased root formation in others.
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